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The map label for this gene is rppH [H]

Identifier: 150398213

GI number: 150398213

Start: 3167408

End: 3167935

Strand: Direct

Name: rppH [H]

Synonym: Smed_3018

Alternate gene names: 150398213

Gene position: 3167408-3167935 (Clockwise)

Preceding gene: 150398212

Following gene: 150398219

Centisome position: 83.75

GC content: 62.69

Gene sequence:

>528_bases
ATGGGCAACGAAAAGGGGCGCGTCATGAAGGCAGAGGACCTGCCCTACCGGCCATGCGTGGGCGCCATGGTTCTCAACCA
GCAGGGACTGGTCTGGGTCGGGCATCGGCTCGCCGTCGGCAATTCGGAATATGACGGGTCGCCGCAGCTCTGGCAGATGC
CGCAAGGGGGCATCGACGAGGGAGAGGACCCGCTCGAGGCCGCCTACCGCGAACTTTACGAAGAGACCGGCATCCGCTCG
GTTTCGCTCATCGCCGAGGCGCCGGACTGGATCAATTACGACCTTCCTTCGCACCTGATCGGGATCGGACTCAAGGGCAA
GTATCGCGGGCAGAGGCAGCGCTGGTACGCCTTCCGCTTCGAGGGCGACGAAAGCGAGATCGCCATCAACCCGCCGCCCG
GCGGTCATGAGCCGGAATTCGACGCCTGGGAATGGAAGCCCATGCATGCGCTGCCAGGTTTGATCGTTCCCTTCAAACGC
AAAGCCTATGAAGAGATCGTCGCGACGTTCTCGCATCTGGTGCGTTGA

Upstream 100 bases:

>100_bases
AGAATCGTTTCCATTTAACCAGGAAAGGCTCTAAGCAGCGGCCTTGCGCGCCTGATGAGGCGCCGCAGCAGACGAACATG
CAAAGGAAGGATGACACGAC

Downstream 100 bases:

>100_bases
GAAGAAAAGGGCGGCACGGCCCCTCATCCGGCCACCGGCCACCTTCTTCCCTCAAGGCGGTTCCTTCTCCCCGTATAACG
GGGGAGAAGGTGGCGGCAGC

Product: dinucleoside polyphosphate hydrolase

Products: NA

Alternate protein names: (Di)nucleoside polyphosphate hydrolase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MGNEKGRVMKAEDLPYRPCVGAMVLNQQGLVWVGHRLAVGNSEYDGSPQLWQMPQGGIDEGEDPLEAAYRELYEETGIRS
VSLIAEAPDWINYDLPSHLIGIGLKGKYRGQRQRWYAFRFEGDESEIAINPPPGGHEPEFDAWEWKPMHALPGLIVPFKR
KAYEEIVATFSHLVR

Sequences:

>Translated_175_residues
MGNEKGRVMKAEDLPYRPCVGAMVLNQQGLVWVGHRLAVGNSEYDGSPQLWQMPQGGIDEGEDPLEAAYRELYEETGIRS
VSLIAEAPDWINYDLPSHLIGIGLKGKYRGQRQRWYAFRFEGDESEIAINPPPGGHEPEFDAWEWKPMHALPGLIVPFKR
KAYEEIVATFSHLVR
>Mature_174_residues
GNEKGRVMKAEDLPYRPCVGAMVLNQQGLVWVGHRLAVGNSEYDGSPQLWQMPQGGIDEGEDPLEAAYRELYEETGIRSV
SLIAEAPDWINYDLPSHLIGIGLKGKYRGQRQRWYAFRFEGDESEIAINPPPGGHEPEFDAWEWKPMHALPGLIVPFKRK
AYEEIVATFSHLVR

Specific function: Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789194, Length=165, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- InterPro:   IPR022927 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 19836; Mature: 19704

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNEKGRVMKAEDLPYRPCVGAMVLNQQGLVWVGHRLAVGNSEYDGSPQLWQMPQGGIDE
CCCCCCCEEECCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCC
GEDPLEAAYRELYEETGIRSVSLIAEAPDWINYDLPSHLIGIGLKGKYRGQRQRWYAFRF
CCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHEEECCCCCCCCCCCEEEEEEE
EGDESEIAINPPPGGHEPEFDAWEWKPMHALPGLIVPFKRKAYEEIVATFSHLVR
ECCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNEKGRVMKAEDLPYRPCVGAMVLNQQGLVWVGHRLAVGNSEYDGSPQLWQMPQGGIDE
CCCCCCEEECCCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCC
GEDPLEAAYRELYEETGIRSVSLIAEAPDWINYDLPSHLIGIGLKGKYRGQRQRWYAFRF
CCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHEEECCCCCCCCCCCEEEEEEE
EGDESEIAINPPPGGHEPEFDAWEWKPMHALPGLIVPFKRKAYEEIVATFSHLVR
ECCCCEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]