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The map label for this gene is phaZ1 [H]

Identifier: 150398188

GI number: 150398188

Start: 3137036

End: 3138295

Strand: Direct

Name: phaZ1 [H]

Synonym: Smed_2991

Alternate gene names: 150398188

Gene position: 3137036-3138295 (Clockwise)

Preceding gene: 150398187

Following gene: 150398196

Centisome position: 82.95

GC content: 63.65

Gene sequence:

>1260_bases
ATGAATGACGATTTCGAAACGGCCATGCGCCGTGCGTTACGGTCCACGCGCGCCTTGAACATTGCCGAGGCGACAAGGGT
GATCCAGGATGCGCTCGCCAGGCGATCGGTCTCCGTTACTCGCTCCTCGTCACCGGACATCACGCCACCGCCGCCAGTTC
AGCGTTCTGCCCCCTTCCCGGTCGATCCGCTTGCCGAGATCGTTGAGCCAGCGACATGGTCTGAGGCCGAAGAGTCCCCC
CAAAAAGTTGGCGATGGAACGTCGTCGCCAAGGTTGCGGAAGCCCTTGCGAGAAACGGTACGGATCTTGCGCGAGAGTCG
GTTGGCGCGCGGCGTTTCTGGATCGCTACGCGGCGTCGGCCTGCCGGGCACGGCAAGACAGGCCCAAGAGCCCGTCATCC
CGGATGGGGCACAGTTCCTGGCACGATCCTTCACCTGCCCCGCGGGTTCGCGAAGCTACAGGCTCTACGTTCCGGCGTCC
GCATCCGATCGGCCGCGCGGCCTTGTAGTCATGCTCCATGGCTGCAAGCAGGATCCCGAAGATTTTGCTGCAGGAACCGG
CATGAACGCGGTTGCCGAGGCACACGGTCTCATGGTTGCGTATCCCCGTCAAAGCGGTGCTGACAATGCGTCGTACTGCT
GGAACTGGTTCAGGCCGGCTGACCAGATGCGCGACGCGGGAGAACCTTCGATCATTGCCGGGATCACCAAGGAGATCATG
TCGGAATCCGGCCTCGATCGCAGCCGAGTCTTCGTGGCAGGCCTATCGGCAGGGGGGGCCATGGCGGCGGTCATGGGCGA
GACCTATCCCGATCTCTATTCAGCCGCGGGAATCCATTCCGGCCTGGCCTACGGATCGGCCAACGACGTCATGTCGGCCT
TCTCCGCGATGCGCGGCGACGGAGGTCTTGCATCCCGTCCGAAGCGGCTCGCTAATGGTCACCGTCTCCGGACGATCGTC
TTCCAGGGAAGTGAGGATCGGACGGTCCATCCCTCCAACGCCGATCGGATCGTCGCCGCGGCAGCGCCTACCGGCCCGGG
ATGTGCGCTCCGTAAGGAATCCGGCCGCTCCGCGAGCGGGCGGATCTATACAAGAACCATCGTAGAGGATTCCGGGGGAA
GCCCGGCGGTCGAATATTGGCTGGTGGAAGGCGCCGGCCATGCCTGGTCCGGCGGCCATCCCTCGGGGACGTATACGGAT
TCGCATGGTCCGGATGCCTCTCGTCAAATGGTGCGCTTCTTCCTCGATGAGCAGGAATGA

Upstream 100 bases:

>100_bases
CATCACCGTGCTCGGCGCCCTCCAAGCCAGCACCGAGCTGAAATCAGCTCTGGCCGAACGCATCCGATAGAACTGCAACC
GGACCAGAAAGGACAACTCC

Downstream 100 bases:

>100_bases
AGGCGCTCTACCGCCGGACGAATGCACCCGTGCCGGTGCTGGCCCGTCAGTCATAAGGGCTTGCGACGCCACCGCAGCTT
CTGCTTCAAGTCTTGTCGAC

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNDDFETAMRRALRSTRALNIAEATRVIQDALARRSVSVTRSSSPDITPPPPVQRSAPFPVDPLAEIVEPATWSEAEESP
QKVGDGTSSPRLRKPLRETVRILRESRLARGVSGSLRGVGLPGTARQAQEPVIPDGAQFLARSFTCPAGSRSYRLYVPAS
ASDRPRGLVVMLHGCKQDPEDFAAGTGMNAVAEAHGLMVAYPRQSGADNASYCWNWFRPADQMRDAGEPSIIAGITKEIM
SESGLDRSRVFVAGLSAGGAMAAVMGETYPDLYSAAGIHSGLAYGSANDVMSAFSAMRGDGGLASRPKRLANGHRLRTIV
FQGSEDRTVHPSNADRIVAAAAPTGPGCALRKESGRSASGRIYTRTIVEDSGGSPAVEYWLVEGAGHAWSGGHPSGTYTD
SHGPDASRQMVRFFLDEQE

Sequences:

>Translated_419_residues
MNDDFETAMRRALRSTRALNIAEATRVIQDALARRSVSVTRSSSPDITPPPPVQRSAPFPVDPLAEIVEPATWSEAEESP
QKVGDGTSSPRLRKPLRETVRILRESRLARGVSGSLRGVGLPGTARQAQEPVIPDGAQFLARSFTCPAGSRSYRLYVPAS
ASDRPRGLVVMLHGCKQDPEDFAAGTGMNAVAEAHGLMVAYPRQSGADNASYCWNWFRPADQMRDAGEPSIIAGITKEIM
SESGLDRSRVFVAGLSAGGAMAAVMGETYPDLYSAAGIHSGLAYGSANDVMSAFSAMRGDGGLASRPKRLANGHRLRTIV
FQGSEDRTVHPSNADRIVAAAAPTGPGCALRKESGRSASGRIYTRTIVEDSGGSPAVEYWLVEGAGHAWSGGHPSGTYTD
SHGPDASRQMVRFFLDEQE
>Mature_419_residues
MNDDFETAMRRALRSTRALNIAEATRVIQDALARRSVSVTRSSSPDITPPPPVQRSAPFPVDPLAEIVEPATWSEAEESP
QKVGDGTSSPRLRKPLRETVRILRESRLARGVSGSLRGVGLPGTARQAQEPVIPDGAQFLARSFTCPAGSRSYRLYVPAS
ASDRPRGLVVMLHGCKQDPEDFAAGTGMNAVAEAHGLMVAYPRQSGADNASYCWNWFRPADQMRDAGEPSIIAGITKEIM
SESGLDRSRVFVAGLSAGGAMAAVMGETYPDLYSAAGIHSGLAYGSANDVMSAFSAMRGDGGLASRPKRLANGHRLRTIV
FQGSEDRTVHPSNADRIVAAAAPTGPGCALRKESGRSASGRIYTRTIVEDSGGSPAVEYWLVEGAGHAWSGGHPSGTYTD
SHGPDASRQMVRFFLDEQE

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 44641; Mature: 44641

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDDFETAMRRALRSTRALNIAEATRVIQDALARRSVSVTRSSSPDITPPPPVQRSAPFP
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCC
VDPLAEIVEPATWSEAEESPQKVGDGTSSPRLRKPLRETVRILRESRLARGVSGSLRGVG
CCHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LPGTARQAQEPVIPDGAQFLARSFTCPAGSRSYRLYVPASASDRPRGLVVMLHGCKQDPE
CCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCHH
DFAAGTGMNAVAEAHGLMVAYPRQSGADNASYCWNWFRPADQMRDAGEPSIIAGITKEIM
HHHCCCCCHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
SESGLDRSRVFVAGLSAGGAMAAVMGETYPDLYSAAGIHSGLAYGSANDVMSAFSAMRGD
HHCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GGLASRPKRLANGHRLRTIVFQGSEDRTVHPSNADRIVAAAAPTGPGCALRKESGRSASG
CCCCCCCHHHHCCCEEEEEEEECCCCCEECCCCCCEEEEEECCCCCCCEEECCCCCCCCC
RIYTRTIVEDSGGSPAVEYWLVEGAGHAWSGGHPSGTYTDSHGPDASRQMVRFFLDEQE
EEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDDFETAMRRALRSTRALNIAEATRVIQDALARRSVSVTRSSSPDITPPPPVQRSAPFP
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCC
VDPLAEIVEPATWSEAEESPQKVGDGTSSPRLRKPLRETVRILRESRLARGVSGSLRGVG
CCHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LPGTARQAQEPVIPDGAQFLARSFTCPAGSRSYRLYVPASASDRPRGLVVMLHGCKQDPE
CCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCHH
DFAAGTGMNAVAEAHGLMVAYPRQSGADNASYCWNWFRPADQMRDAGEPSIIAGITKEIM
HHHCCCCCHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
SESGLDRSRVFVAGLSAGGAMAAVMGETYPDLYSAAGIHSGLAYGSANDVMSAFSAMRGD
HHCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GGLASRPKRLANGHRLRTIVFQGSEDRTVHPSNADRIVAAAAPTGPGCALRKESGRSASG
CCCCCCCHHHHCCCEEEEEEEECCCCCEECCCCCCEEEEEECCCCCCCEEECCCCCCCCC
RIYTRTIVEDSGGSPAVEYWLVEGAGHAWSGGHPSGTYTDSHGPDASRQMVRFFLDEQE
EEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]