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The map label for this gene is dhaAF [H]

Identifier: 150398084

GI number: 150398084

Start: 3022256

End: 3023128

Strand: Direct

Name: dhaAF [H]

Synonym: Smed_2886

Alternate gene names: 150398084

Gene position: 3022256-3023128 (Clockwise)

Preceding gene: 150398082

Following gene: 150398085

Centisome position: 79.91

GC content: 60.6

Gene sequence:

>873_bases
ATGAAGTCAGAAATTTCCGTAAAACACCGTTTCGCCGATTTGGACGGCCTACGCGTCTTCTATCGCGAAGCCGGACCGAA
GGACGCGCCGGTAGTGCTCCTCCCGCACGGCTATCCCTGCTCGTCATACGAGTTCCGCAATTTCATGCCGCGCCTGGCCG
ACCGCTGGAGGCTCCTCGCTCCGGATTTTCCCGGGTCGGGATATAGCGACACGCCGGGCGGGTTCGCCTACGACTTCGAC
GGGTTCACCGATTTGCTCGATGCCTTCATCCGGCGCCTGGGCCTGGAGAGATTCGCACTTTATTTGCATGATTTCGGATC
GCAGATCGGGCTTCGGCTGGCGATGCGCCGTCCTGAGCGGATTACGGCGCTTATCATTCAAAATGGCGACATCTACGAAG
ATGAGCTTGGTCCGAAATACGCGCCGTTGCAGGCCTATTTCCGCAATCCTACGCCGGAAGGCCGATCCATGCTCGGTAAA
GCCGTCAGCGAGGAGGGATACCGCGACGAGTTTCTCAACGATGTCCGGCCCGAACTTGCCGAACGGATTTCTCCCGACCT
CTGGAAACTTCATTGGTCGCTGACGACGGCCCGGCGGCGGGAGATCGCGATAGACGTCATCGCCGGGTTGCGGGAAAATC
TCGCATGGTTCCCCCGCTATCAGGCCTATCTGCATGAGCATCGGCCGCCTACGCTGATCGTCTGGGGTCCACAGGACGGC
TACATGCCCGAGGGCTCTGCTCGAGCCTATCTCCGCGATCTGCCCGACGCCGAATTGCATCTGCTCGACGGAGGCCATTG
GCTGCTGGAGACGAACCTGTCCGAGGTCGTTTCCCTCTCACGGGATTTCCTCACGCGCGCGGCACCGGGGTGA

Upstream 100 bases:

>100_bases
TTGTAAAGGAGCCGACACGGTACTTCTATGTCAACGGCCACGGAACCATCGCTTGCATACAGTGTTCGACCTCGTCGAAC
CATTACGAGGTGCCGCGACG

Downstream 100 bases:

>100_bases
GACACCCCTCCTCCGATCTCAGCTGGCTTCCCGCCCTTACGAGGTTTGCGCTTATGCTGCATAAGCGCAAACTTCGGCCT
TGTGCCTGATGCATCACAGA

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKSEISVKHRFADLDGLRVFYREAGPKDAPVVLLPHGYPCSSYEFRNFMPRLADRWRLLAPDFPGSGYSDTPGGFAYDFD
GFTDLLDAFIRRLGLERFALYLHDFGSQIGLRLAMRRPERITALIIQNGDIYEDELGPKYAPLQAYFRNPTPEGRSMLGK
AVSEEGYRDEFLNDVRPELAERISPDLWKLHWSLTTARRREIAIDVIAGLRENLAWFPRYQAYLHEHRPPTLIVWGPQDG
YMPEGSARAYLRDLPDAELHLLDGGHWLLETNLSEVVSLSRDFLTRAAPG

Sequences:

>Translated_290_residues
MKSEISVKHRFADLDGLRVFYREAGPKDAPVVLLPHGYPCSSYEFRNFMPRLADRWRLLAPDFPGSGYSDTPGGFAYDFD
GFTDLLDAFIRRLGLERFALYLHDFGSQIGLRLAMRRPERITALIIQNGDIYEDELGPKYAPLQAYFRNPTPEGRSMLGK
AVSEEGYRDEFLNDVRPELAERISPDLWKLHWSLTTARRREIAIDVIAGLRENLAWFPRYQAYLHEHRPPTLIVWGPQDG
YMPEGSARAYLRDLPDAELHLLDGGHWLLETNLSEVVSLSRDFLTRAAPG
>Mature_290_residues
MKSEISVKHRFADLDGLRVFYREAGPKDAPVVLLPHGYPCSSYEFRNFMPRLADRWRLLAPDFPGSGYSDTPGGFAYDFD
GFTDLLDAFIRRLGLERFALYLHDFGSQIGLRLAMRRPERITALIIQNGDIYEDELGPKYAPLQAYFRNPTPEGRSMLGK
AVSEEGYRDEFLNDVRPELAERISPDLWKLHWSLTTARRREIAIDVIAGLRENLAWFPRYQAYLHEHRPPTLIVWGPQDG
YMPEGSARAYLRDLPDAELHLLDGGHWLLETNLSEVVSLSRDFLTRAAPG

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI29294639, Length=270, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI29294637, Length=270, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI29294635, Length=270, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562298, Length=287, Percent_Identity=24.0418118466899, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324392, Length=292, Percent_Identity=31.1643835616438, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 33242; Mature: 33242

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEISVKHRFADLDGLRVFYREAGPKDAPVVLLPHGYPCSSYEFRNFMPRLADRWRLLA
CCCCCCHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEC
PDFPGSGYSDTPGGFAYDFDGFTDLLDAFIRRLGLERFALYLHDFGSQIGLRLAMRRPER
CCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCE
ITALIIQNGDIYEDELGPKYAPLQAYFRNPTPEGRSMLGKAVSEEGYRDEFLNDVRPELA
EEEEEEECCCEEHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ERISPDLWKLHWSLTTARRREIAIDVIAGLRENLAWFPRYQAYLHEHRPPTLIVWGPQDG
HHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
YMPEGSARAYLRDLPDAELHLLDGGHWLLETNLSEVVSLSRDFLTRAAPG
CCCCCCHHHHHHCCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSEISVKHRFADLDGLRVFYREAGPKDAPVVLLPHGYPCSSYEFRNFMPRLADRWRLLA
CCCCCCHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEC
PDFPGSGYSDTPGGFAYDFDGFTDLLDAFIRRLGLERFALYLHDFGSQIGLRLAMRRPER
CCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCE
ITALIIQNGDIYEDELGPKYAPLQAYFRNPTPEGRSMLGKAVSEEGYRDEFLNDVRPELA
EEEEEEECCCEEHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ERISPDLWKLHWSLTTARRREIAIDVIAGLRENLAWFPRYQAYLHEHRPPTLIVWGPQDG
HHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
YMPEGSARAYLRDLPDAELHLLDGGHWLLETNLSEVVSLSRDFLTRAAPG
CCCCCCHHHHHHCCCCCEEEEEECCCEEEECCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]