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The map label for this gene is phaD [H]

Identifier: 150398006

GI number: 150398006

Start: 2934621

End: 2936240

Strand: Direct

Name: phaD [H]

Synonym: Smed_2808

Alternate gene names: 150398006

Gene position: 2934621-2936240 (Clockwise)

Preceding gene: 150398005

Following gene: 150398007

Centisome position: 77.6

GC content: 62.65

Gene sequence:

>1620_bases
GTGACTGATTGGCTCCATCATCTTCTCATATTGCCGATCCTCCTGCCGCTCGCCGTCGCCGCGGTTCTGATCCCGATCAA
CGAGCGGGACCGCACAGTGAAGGGCGCCATAGGATTCGGTTCAACGCTCGTCGTATTCATCCTTTCGATGATCCTCATGC
GGTTTGCCGCTTCCGGAACCGCCAGCCTGCCTGGATCGGGGGTTTACCAGCTCGGCAACTGGCCCGCCCCCTTTGGCATC
GTCCTCGTTCTGGATCGCCTGTCGGCTCTGATGCTGTGCCTGACGAGTGGACTTGCACTCGCCGCGCAGGCCTATTCGAT
GGCGCGCTGGCACACGGCGGGACATCACTTCCATTCGCTGTTCCAGCTCCTCCTTGCAGGCCTCAACGGCGCCTTTCTCA
CGGGCGACCTCTTCAATCTCTTCGTCTTCTTCGAAATGATGCTGGCCGCCTCCTACGGACTGCTCCTGCACGGCTCCGGA
CCTATACGCGTGAAGGCGGGCCTGCATTATGTTGCGGTCAATCTCGCCGCTTCGTCACTGTTCCTGATCGGCGTCAGCCT
GATCTACGGTACGACCGGCACCCTCAACATGGCCGACCTCGCAACGAAGATCGCAGCGATCGACGCCCAAGACAGAACAT
TTGTCGAAATGGGGGCAGCCATTCTCGGCGTCGCCTTCCTCGTTAAGGCCGGCATGTGGCCGCTCAGCTTCTGGCTGCCA
ACGGCCTACGCGGCGGCAACGCCGCCGGTCGCCGGAATCTTCGCAATCCTGACCAAGGTCGGCATCTATGTCATTATGCG
CCTGCATCTGCTCGTCTTCGGCACGACAGCGGGCGCGTCGTCCGGTTTCGGCCAGGACTGGCTGGTCACCGGGGGAATGC
TGACGATCGCCTTCGGCGCCATAGGCGTGCTCGCCTCGCAGGCAATGGGTCGCCTTGCCGGCTACTCCGTTCTGGTATCC
TCCGGGACGCTGCTCGCGGCGATCGGCCTTGGCCACGGCGGAATGCTCGCCGGTGCTCTCTTCTATCTGGTCAGCTCGAC
GCTGACGATCGCGGCCTTTTTCCTGCTGATCGAGCTCGTCGAGCGCGGTCGGGACGCCGGCGCCGACGTCCTCGCTGTGA
CGATGGAAGCTTATGGCGATTTCGACGAAGACGAAGAAGAGGAAGAGGTCGGCGTTGCCATTCCGGGTACCATGGCCGTT
CTCGGTCTCTGCTTCTGCCTCTGCGCATTGCTGCTCGCCGGCCTGCCGCCTTTCTCGGGTTTCATCGCCAAGTTTGCGCT
CATAAGCGGCCTATTCGATGCGCCGGCCGCCGATCTTGCCGCAGCAACCTCCGTCGCGGACTGGATCTATGTCGCCCTCC
TCATCCTGTCAGGGCTCGCCGCCATGATCGCAATGAACCGGATCGGCATCCGCACTTTCTGGGCATCGATCGAGGGGACG
ATACCGCGGGTTGTCGTGATCGAGATCACACCCGTGATTGTCCTGCTTGCCGCCTGCATCTTCCTGAGCCTGCAGGCGGG
CCCCGCGATGCGCTACATGCAGGCGACGGCCGACGATCTGCTCGCTCCGCTCACCTTGAGCGATCGGGTGCTTTCGGCGC
CGCGAGCCGGGAGCAATTAG

Upstream 100 bases:

>100_bases
ATCGTCATCGGCTTCGCGATGACAGCACTGTTCCTCGTGGTACTGCTTGCCTCGCGCGGCTTCACCGGAACCGACCATGT
GGACGGAAGGGAGCAGCGCG

Downstream 100 bases:

>100_bases
AGATGCGCACCTGGTTCCCCTATCCGCTGCTCTCCGTCGCGCTCCTCCTCATGTGGCTGCTTCTAAGTCAGTCCGTGACG
CCGGGTTCGATCCTCCTCGG

Product: putative monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTDWLHHLLILPILLPLAVAAVLIPINERDRTVKGAIGFGSTLVVFILSMILMRFAASGTASLPGSGVYQLGNWPAPFGI
VLVLDRLSALMLCLTSGLALAAQAYSMARWHTAGHHFHSLFQLLLAGLNGAFLTGDLFNLFVFFEMMLAASYGLLLHGSG
PIRVKAGLHYVAVNLAASSLFLIGVSLIYGTTGTLNMADLATKIAAIDAQDRTFVEMGAAILGVAFLVKAGMWPLSFWLP
TAYAAATPPVAGIFAILTKVGIYVIMRLHLLVFGTTAGASSGFGQDWLVTGGMLTIAFGAIGVLASQAMGRLAGYSVLVS
SGTLLAAIGLGHGGMLAGALFYLVSSTLTIAAFFLLIELVERGRDAGADVLAVTMEAYGDFDEDEEEEEVGVAIPGTMAV
LGLCFCLCALLLAGLPPFSGFIAKFALISGLFDAPAADLAAATSVADWIYVALLILSGLAAMIAMNRIGIRTFWASIEGT
IPRVVVIEITPVIVLLAACIFLSLQAGPAMRYMQATADDLLAPLTLSDRVLSAPRAGSN

Sequences:

>Translated_539_residues
MTDWLHHLLILPILLPLAVAAVLIPINERDRTVKGAIGFGSTLVVFILSMILMRFAASGTASLPGSGVYQLGNWPAPFGI
VLVLDRLSALMLCLTSGLALAAQAYSMARWHTAGHHFHSLFQLLLAGLNGAFLTGDLFNLFVFFEMMLAASYGLLLHGSG
PIRVKAGLHYVAVNLAASSLFLIGVSLIYGTTGTLNMADLATKIAAIDAQDRTFVEMGAAILGVAFLVKAGMWPLSFWLP
TAYAAATPPVAGIFAILTKVGIYVIMRLHLLVFGTTAGASSGFGQDWLVTGGMLTIAFGAIGVLASQAMGRLAGYSVLVS
SGTLLAAIGLGHGGMLAGALFYLVSSTLTIAAFFLLIELVERGRDAGADVLAVTMEAYGDFDEDEEEEEVGVAIPGTMAV
LGLCFCLCALLLAGLPPFSGFIAKFALISGLFDAPAADLAAATSVADWIYVALLILSGLAAMIAMNRIGIRTFWASIEGT
IPRVVVIEITPVIVLLAACIFLSLQAGPAMRYMQATADDLLAPLTLSDRVLSAPRAGSN
>Mature_538_residues
TDWLHHLLILPILLPLAVAAVLIPINERDRTVKGAIGFGSTLVVFILSMILMRFAASGTASLPGSGVYQLGNWPAPFGIV
LVLDRLSALMLCLTSGLALAAQAYSMARWHTAGHHFHSLFQLLLAGLNGAFLTGDLFNLFVFFEMMLAASYGLLLHGSGP
IRVKAGLHYVAVNLAASSLFLIGVSLIYGTTGTLNMADLATKIAAIDAQDRTFVEMGAAILGVAFLVKAGMWPLSFWLPT
AYAAATPPVAGIFAILTKVGIYVIMRLHLLVFGTTAGASSGFGQDWLVTGGMLTIAFGAIGVLASQAMGRLAGYSVLVSS
GTLLAAIGLGHGGMLAGALFYLVSSTLTIAAFFLLIELVERGRDAGADVLAVTMEAYGDFDEDEEEEEVGVAIPGTMAVL
GLCFCLCALLLAGLPPFSGFIAKFALISGLFDAPAADLAAATSVADWIYVALLILSGLAAMIAMNRIGIRTFWASIEGTI
PRVVVIEITPVIVLLAACIFLSLQAGPAMRYMQATADDLLAPLTLSDRVLSAPRAGSN

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=473, Percent_Identity=25.369978858351, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788829, Length=467, Percent_Identity=23.982869379015, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788613, Length=313, Percent_Identity=31.629392971246, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788831, Length=274, Percent_Identity=25.9124087591241, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 56375; Mature: 56244

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDWLHHLLILPILLPLAVAAVLIPINERDRTVKGAIGFGSTLVVFILSMILMRFAASGT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
ASLPGSGVYQLGNWPAPFGIVLVLDRLSALMLCLTSGLALAAQAYSMARWHTAGHHFHSL
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQLLLAGLNGAFLTGDLFNLFVFFEMMLAASYGLLLHGSGPIRVKAGLHYVAVNLAASSL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCEEHHHHHHHHH
FLIGVSLIYGTTGTLNMADLATKIAAIDAQDRTFVEMGAAILGVAFLVKAGMWPLSFWLP
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TAYAAATPPVAGIFAILTKVGIYVIMRLHLLVFGTTAGASSGFGQDWLVTGGMLTIAFGA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHH
IGVLASQAMGRLAGYSVLVSSGTLLAAIGLGHGGMLAGALFYLVSSTLTIAAFFLLIELV
HHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGRDAGADVLAVTMEAYGDFDEDEEEEEVGVAIPGTMAVLGLCFCLCALLLAGLPPFSG
HCCCCCCCHHHEEEHHHHCCCCCCCCHHHCCEECCHHHHHHHHHHHHHHHHHCCCCCHHH
FIAKFALISGLFDAPAADLAAATSVADWIYVALLILSGLAAMIAMNRIGIRTFWASIEGT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPRVVVIEITPVIVLLAACIFLSLQAGPAMRYMQATADDLLAPLTLSDRVLSAPRAGSN
CCEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
TDWLHHLLILPILLPLAVAAVLIPINERDRTVKGAIGFGSTLVVFILSMILMRFAASGT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
ASLPGSGVYQLGNWPAPFGIVLVLDRLSALMLCLTSGLALAAQAYSMARWHTAGHHFHSL
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQLLLAGLNGAFLTGDLFNLFVFFEMMLAASYGLLLHGSGPIRVKAGLHYVAVNLAASSL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCEEHHHHHHHHH
FLIGVSLIYGTTGTLNMADLATKIAAIDAQDRTFVEMGAAILGVAFLVKAGMWPLSFWLP
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
TAYAAATPPVAGIFAILTKVGIYVIMRLHLLVFGTTAGASSGFGQDWLVTGGMLTIAFGA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHH
IGVLASQAMGRLAGYSVLVSSGTLLAAIGLGHGGMLAGALFYLVSSTLTIAAFFLLIELV
HHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGRDAGADVLAVTMEAYGDFDEDEEEEEVGVAIPGTMAVLGLCFCLCALLLAGLPPFSG
HCCCCCCCHHHEEEHHHHCCCCCCCCHHHCCEECCHHHHHHHHHHHHHHHHHCCCCCHHH
FIAKFALISGLFDAPAADLAAATSVADWIYVALLILSGLAAMIAMNRIGIRTFWASIEGT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPRVVVIEITPVIVLLAACIFLSLQAGPAMRYMQATADDLLAPLTLSDRVLSAPRAGSN
CCEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]