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The map label for this gene is rhtB [H]

Identifier: 150397968

GI number: 150397968

Start: 2889978

End: 2890679

Strand: Direct

Name: rhtB [H]

Synonym: Smed_2770

Alternate gene names: 150397968

Gene position: 2889978-2890679 (Clockwise)

Preceding gene: 150397966

Following gene: 150397971

Centisome position: 76.42

GC content: 62.68

Gene sequence:

>702_bases
ATGTCGGATCGGCGCTTGCGCGCACGTCATAGCTGTGGGACCAGTTGTGACCAAAGGAAGGACATAATGAGCGAAGTCAC
CATTCTCGCATTCGCCCTCATCGCTTTCGTCGGCATAGCGACACCCGGACCGACCGTGCTTCTCGCGCTGACGAATGGTT
CGCGCTACGGCGTGAAGCGCGCAACCCCAGGGATGATTGGCGCGGTCCTCTCGGATTTCGTCCTGATCGGCGCCGTGGCG
GTCGGGCTCGGCGCGCTGCTCGCGGCATCGGAGTTCTGGTTCTCCGTGGTCAAATGGTTAGGCGTCGCTTATCTCGCTTT
TCTCGGGGTCACGCTGCTGCGCTCCCGCGGTACGCTGGACCTCCCGTTGCAAGGGTCGCAGGGCACGGAATCCGGCGCAA
CGCGCTCCATCTTTATCAAGAGCTTCCTCGTCGCCATCACCAATCCCAAAGGCTATCTGTTCTTTTCGGCCTTTCTGCCG
CAATTCATCGAACCCGCCCTGCCGCAGGCGCCGCAATACGCCATGCTGGCCACCGTCTTCGCATCGATCGATTTCGCAGT
GATGTTCGGTTACGCCCTGCTTGGGTCGCAGGCGGTGCGCTTCATGAGGCGCTCAGGCTCGATCTGGCTCGACCGCATCT
GCGGCGGCGCGTTGCTGGCGCTCGCGGGATCCCTGGCGCTCTATCGGCGTGCTGCGAGCTGA

Upstream 100 bases:

>100_bases
GGGGCTTCACCGAAGCTTTTCGCTCGCGGACGGCAGGGGCCCTCGCCCGGGACCTCCATAGGACAAGCCACCGATACTGT
CAAACGGGGGCGCCCACAAC

Downstream 100 bases:

>100_bases
ACGCCTGAATCCAGGCGCTCAGGCCGCGCGGCGAAGCGGCGGCAAAGCGAGTTTGCGGCCGCTGGCCACCAGCATGCCGG
CAGCGCCGTCGAGCCAGCCG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MSDRRLRARHSCGTSCDQRKDIMSEVTILAFALIAFVGIATPGPTVLLALTNGSRYGVKRATPGMIGAVLSDFVLIGAVA
VGLGALLAASEFWFSVVKWLGVAYLAFLGVTLLRSRGTLDLPLQGSQGTESGATRSIFIKSFLVAITNPKGYLFFSAFLP
QFIEPALPQAPQYAMLATVFASIDFAVMFGYALLGSQAVRFMRRSGSIWLDRICGGALLALAGSLALYRRAAS

Sequences:

>Translated_233_residues
MSDRRLRARHSCGTSCDQRKDIMSEVTILAFALIAFVGIATPGPTVLLALTNGSRYGVKRATPGMIGAVLSDFVLIGAVA
VGLGALLAASEFWFSVVKWLGVAYLAFLGVTLLRSRGTLDLPLQGSQGTESGATRSIFIKSFLVAITNPKGYLFFSAFLP
QFIEPALPQAPQYAMLATVFASIDFAVMFGYALLGSQAVRFMRRSGSIWLDRICGGALLALAGSLALYRRAAS
>Mature_232_residues
SDRRLRARHSCGTSCDQRKDIMSEVTILAFALIAFVGIATPGPTVLLALTNGSRYGVKRATPGMIGAVLSDFVLIGAVAV
GLGALLAASEFWFSVVKWLGVAYLAFLGVTLLRSRGTLDLPLQGSQGTESGATRSIFIKSFLVAITNPKGYLFFSAFLPQ
FIEPALPQAPQYAMLATVFASIDFAVMFGYALLGSQAVRFMRRSGSIWLDRICGGALLALAGSLALYRRAAS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=203, Percent_Identity=32.512315270936, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788099, Length=131, Percent_Identity=29.7709923664122, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 24826; Mature: 24694

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRRLRARHSCGTSCDQRKDIMSEVTILAFALIAFVGIATPGPTVLLALTNGSRYGVKR
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
ATPGMIGAVLSDFVLIGAVAVGLGALLAASEFWFSVVKWLGVAYLAFLGVTLLRSRGTLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LPLQGSQGTESGATRSIFIKSFLVAITNPKGYLFFSAFLPQFIEPALPQAPQYAMLATVF
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ASIDFAVMFGYALLGSQAVRFMRRSGSIWLDRICGGALLALAGSLALYRRAAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDRRLRARHSCGTSCDQRKDIMSEVTILAFALIAFVGIATPGPTVLLALTNGSRYGVKR
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
ATPGMIGAVLSDFVLIGAVAVGLGALLAASEFWFSVVKWLGVAYLAFLGVTLLRSRGTLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LPLQGSQGTESGATRSIFIKSFLVAITNPKGYLFFSAFLPQFIEPALPQAPQYAMLATVF
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
ASIDFAVMFGYALLGSQAVRFMRRSGSIWLDRICGGALLALAGSLALYRRAAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]