Click here to switch to the map view.

The map label for this gene is puuD [H]

Identifier: 150397960

GI number: 150397960

Start: 2882653

End: 2883408

Strand: Direct

Name: puuD [H]

Synonym: Smed_2762

Alternate gene names: 150397960

Gene position: 2882653-2883408 (Clockwise)

Preceding gene: 150397954

Following gene: 150397964

Centisome position: 76.22

GC content: 64.95

Gene sequence:

>756_bases
ATGTCGAAACCTGTCGTTGCCATTCCTTGCGACTTCCGCGAATTCGACGGCAATGTCTGGCATGTTGCTGCCCACCAATA
TGTCCGCGCTGCAGTTGAGGGCGCTGGCCTGATGGTGTTCCTCGTTCCGGCTCTGGAGACCGGCAACGACGTCGACGAGA
TCCTCGACCGCGTCGACGGCGTGCTGGCAAGCGGCGCGCGCTCCAACGTCCACCCTTCGCTCTATGGCAGGGAGGCGGCT
GAAACAGACGGGCCTTTCGATCCGGGTCGCGATGCGACCAGCCTCCCGCTGATCCGCCGCGCCATCGATCGGGGTATCCC
TCTGTTTGCGATCTGCCGCGGGATACAGGAACTGAACGTCGCGCTAGGCGGCACGCTCGCAACGGAAATCCAGGAGCAGG
CCGGCGTCTGGGACCATCGGAAGCCCGACGTGCCGGAGCTCGACCTTGCCTATGGCATTCGCCAGGACGTGGTGGTCAAC
GAAGGAAGCTGCCTCGCACCCGTCCTCGGTACAGGCCGCATAAGAGTCAATTCGCTGCACCGGCAGGCAATCGCCGCTGC
CGCGCCGCGCCTTGCGGTCGAAGCCCTTGCCGACGACGGGACGATCGAGGCCGTCTCGGTCATCGGCGCCAAGGCCTTCG
CCATCGGCGTTCAGTGGCATCCCGAATACTGGGTACGCACCGACGCCCCATCAGCCGCCCTCTTCAGGGCCTTCGGCGAT
GCCGCACGCGCCTATCGTGAGAGCAAGGCCCGTTAA

Upstream 100 bases:

>100_bases
GACGGGCGGCGTGTTCTGCGCTGTGCGGCCTTTCTCCCACTGTTGACTTTCTGAACTTTCTGACGGCAAGGCTTTTGCGG
AAGATATACGGAGCCTGAAG

Downstream 100 bases:

>100_bases
TTAGACACTTTACGCCGAAACGGCGTCTCGACGACCGGCGTCAACGCCTCGCCGGTCGAGAACCATGCCTTCAGATTGTC
GACGACGAGATCGGACATGG

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSKPVVAIPCDFREFDGNVWHVAAHQYVRAAVEGAGLMVFLVPALETGNDVDEILDRVDGVLASGARSNVHPSLYGREAA
ETDGPFDPGRDATSLPLIRRAIDRGIPLFAICRGIQELNVALGGTLATEIQEQAGVWDHRKPDVPELDLAYGIRQDVVVN
EGSCLAPVLGTGRIRVNSLHRQAIAAAAPRLAVEALADDGTIEAVSVIGAKAFAIGVQWHPEYWVRTDAPSAALFRAFGD
AARAYRESKAR

Sequences:

>Translated_251_residues
MSKPVVAIPCDFREFDGNVWHVAAHQYVRAAVEGAGLMVFLVPALETGNDVDEILDRVDGVLASGARSNVHPSLYGREAA
ETDGPFDPGRDATSLPLIRRAIDRGIPLFAICRGIQELNVALGGTLATEIQEQAGVWDHRKPDVPELDLAYGIRQDVVVN
EGSCLAPVLGTGRIRVNSLHRQAIAAAAPRLAVEALADDGTIEAVSVIGAKAFAIGVQWHPEYWVRTDAPSAALFRAFGD
AARAYRESKAR
>Mature_250_residues
SKPVVAIPCDFREFDGNVWHVAAHQYVRAAVEGAGLMVFLVPALETGNDVDEILDRVDGVLASGARSNVHPSLYGREAAE
TDGPFDPGRDATSLPLIRRAIDRGIPLFAICRGIQELNVALGGTLATEIQEQAGVWDHRKPDVPELDLAYGIRQDVVVNE
GSCLAPVLGTGRIRVNSLHRQAIAAAAPRLAVEALADDGTIEAVSVIGAKAFAIGVQWHPEYWVRTDAPSAALFRAFGDA
ARAYRESKAR

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=253, Percent_Identity=39.1304347826087, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 26926; Mature: 26795

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPVVAIPCDFREFDGNVWHVAAHQYVRAAVEGAGLMVFLVPALETGNDVDEILDRVDG
CCCCEEEECCCHHHHCCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
VLASGARSNVHPSLYGREAAETDGPFDPGRDATSLPLIRRAIDRGIPLFAICRGIQELNV
HHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
ALGGTLATEIQEQAGVWDHRKPDVPELDLAYGIRQDVVVNEGSCLAPVLGTGRIRVNSLH
HHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEECCCCEEEHHHCCCEEEEHHHH
RQAIAAAAPRLAVEALADDGTIEAVSVIGAKAFAIGVQWHPEYWVRTDAPSAALFRAFGD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEEEEECCCEEEECCCCHHHHHHHHHH
AARAYRESKAR
HHHHHHHHCCC
>Mature Secondary Structure 
SKPVVAIPCDFREFDGNVWHVAAHQYVRAAVEGAGLMVFLVPALETGNDVDEILDRVDG
CCCEEEECCCHHHHCCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHH
VLASGARSNVHPSLYGREAAETDGPFDPGRDATSLPLIRRAIDRGIPLFAICRGIQELNV
HHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
ALGGTLATEIQEQAGVWDHRKPDVPELDLAYGIRQDVVVNEGSCLAPVLGTGRIRVNSLH
HHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCEEECCCCEEEHHHCCCEEEEHHHH
RQAIAAAAPRLAVEALADDGTIEAVSVIGAKAFAIGVQWHPEYWVRTDAPSAALFRAFGD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEEEEECCCEEEECCCCHHHHHHHHHH
AARAYRESKAR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]