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The map label for this gene is pldB [H]

Identifier: 150397914

GI number: 150397914

Start: 2830247

End: 2831260

Strand: Direct

Name: pldB [H]

Synonym: Smed_2716

Alternate gene names: 150397914

Gene position: 2830247-2831260 (Clockwise)

Preceding gene: 150397912

Following gene: 150397917

Centisome position: 74.84

GC content: 64.1

Gene sequence:

>1014_bases
ATGAACGCGCGCCTCCATGACCACGCTCCCATGACCACGCTCCTCCAGTCGATGCCGCAAAATCCGCTTCCAGGCGATCC
CGTGGTAGGCTTCTTCGACGGGGCCGATGAGCGCAAGATCCGCTACGCGGTGTTCAAGTCGCGAGCGCCCGTCATTCGCG
GCACGATCGTGCTGCTGCAGGGCCGCAATGAGTCGATCGAGAAATACTTCGAAACGATCGGCGAGCTCATGGCGGCCGGC
TTCTGGGTTGCGACCTTCGACTGGCGCGGACAGGGCGGCTCCGAACGGCTTTCGCCGCACCGGACCCGCGGGCATGTGGA
ACATTTCGCCGATTACGAGCGCGATCTGACGAGATTTCTCGATGAAATCGTCCTGCCCGACACCCGCCTGCCCTTCTCGA
TCGTCGCCCATTCCATGGGCGCGCTCGTCGCCCTGTCGCTCGCCCCCATGCTTGCAAGCCGCATAGACCGAATGGTTCTG
CTCGCCCCCTTCGTCGGCCTGGGCGGTCAGGCGATCAGCGAACGGGGTATCGCGGCCATCGCGACCATCATGCGGTTGCT
CGGTCTTGGAAGGCTGCCGCTGAGTGCCGACAGGGACAAACGCGCGCAGTTCAAGGGCAATGTACTGACCTCGGATGACC
GCCGCTACCGCCGCAACCTGGCACTCATCGATGCGCGTCCTCAATTGCGGGTCGGCCCACCTACAGCCCGCTGGCTGAGC
GAAGCCTTCGCAACTATGCGGCGTGTACTCCGACACGAACATCTGACGAGAATCACCATACCGACCGTCATACTCGCACC
GACCGCCGACAGGCTGGTGCCCTATGTCCCGGTCGAATATCTCGCAAGCAATTTCCGCGCGGGGCATTTGATCCCGATCG
ACGGTGCGCGGCACGAACTCCTGCAGGAGGCCGACCGCTATCGCGCCCAGGCGATGGCCGCAATCTTCGCTTTCCTGCCC
GGTGCGGACGCCGAGGACCCCGGCTCCCTGCCTCGGCAGCTGGAAGAGGCTTAG

Upstream 100 bases:

>100_bases
GGTGATCGTCCGGATTTCATCCACCGTTTCCGGCTTTTTTCTTTGACGGGCGGCGGGCCGCCGCCTATCCCTACTGTATG
TTGCTACTGGCCCAGGCCCT

Downstream 100 bases:

>100_bases
TGCCTTTCGTGCCGACTGGAAAAGCCCTAATCCCCCTTGAGCACCTCCAGCGCCCGCGCATGCACTTCACGGCTGCCGGC
GGCGATGATTTCGCCGCCCA

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Lecithinase B [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNARLHDHAPMTTLLQSMPQNPLPGDPVVGFFDGADERKIRYAVFKSRAPVIRGTIVLLQGRNESIEKYFETIGELMAAG
FWVATFDWRGQGGSERLSPHRTRGHVEHFADYERDLTRFLDEIVLPDTRLPFSIVAHSMGALVALSLAPMLASRIDRMVL
LAPFVGLGGQAISERGIAAIATIMRLLGLGRLPLSADRDKRAQFKGNVLTSDDRRYRRNLALIDARPQLRVGPPTARWLS
EAFATMRRVLRHEHLTRITIPTVILAPTADRLVPYVPVEYLASNFRAGHLIPIDGARHELLQEADRYRAQAMAAIFAFLP
GADAEDPGSLPRQLEEA

Sequences:

>Translated_337_residues
MNARLHDHAPMTTLLQSMPQNPLPGDPVVGFFDGADERKIRYAVFKSRAPVIRGTIVLLQGRNESIEKYFETIGELMAAG
FWVATFDWRGQGGSERLSPHRTRGHVEHFADYERDLTRFLDEIVLPDTRLPFSIVAHSMGALVALSLAPMLASRIDRMVL
LAPFVGLGGQAISERGIAAIATIMRLLGLGRLPLSADRDKRAQFKGNVLTSDDRRYRRNLALIDARPQLRVGPPTARWLS
EAFATMRRVLRHEHLTRITIPTVILAPTADRLVPYVPVEYLASNFRAGHLIPIDGARHELLQEADRYRAQAMAAIFAFLP
GADAEDPGSLPRQLEEA
>Mature_337_residues
MNARLHDHAPMTTLLQSMPQNPLPGDPVVGFFDGADERKIRYAVFKSRAPVIRGTIVLLQGRNESIEKYFETIGELMAAG
FWVATFDWRGQGGSERLSPHRTRGHVEHFADYERDLTRFLDEIVLPDTRLPFSIVAHSMGALVALSLAPMLASRIDRMVL
LAPFVGLGGQAISERGIAAIATIMRLLGLGRLPLSADRDKRAQFKGNVLTSDDRRYRRNLALIDARPQLRVGPPTARWLS
EAFATMRRVLRHEHLTRITIPTVILAPTADRLVPYVPVEYLASNFRAGHLIPIDGARHELLQEADRYRAQAMAAIFAFLP
GADAEDPGSLPRQLEEA

Specific function: Unknown

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994980, Length=297, Percent_Identity=32.3232323232323, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 37552; Mature: 37552

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARLHDHAPMTTLLQSMPQNPLPGDPVVGFFDGADERKIRYAVFKSRAPVIRGTIVLLQ
CCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEEEEEE
GRNESIEKYFETIGELMAAGFWVATFDWRGQGGSERLSPHRTRGHVEHFADYERDLTRFL
CCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DEIVLPDTRLPFSIVAHSMGALVALSLAPMLASRIDRMVLLAPFVGLGGQAISERGIAAI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ATIMRLLGLGRLPLSADRDKRAQFKGNVLTSDDRRYRRNLALIDARPQLRVGPPTARWLS
HHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHCEEEEECCCCCEECCCHHHHHH
EAFATMRRVLRHEHLTRITIPTVILAPTADRLVPYVPVEYLASNFRAGHLIPIDGARHEL
HHHHHHHHHHHHHHHHEEEECEEEECCCHHHCCCCCCHHHHHCCCCCCCEEECCCHHHHH
LQEADRYRAQAMAAIFAFLPGADAEDPGSLPRQLEEA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNARLHDHAPMTTLLQSMPQNPLPGDPVVGFFDGADERKIRYAVFKSRAPVIRGTIVLLQ
CCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEEEEEE
GRNESIEKYFETIGELMAAGFWVATFDWRGQGGSERLSPHRTRGHVEHFADYERDLTRFL
CCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DEIVLPDTRLPFSIVAHSMGALVALSLAPMLASRIDRMVLLAPFVGLGGQAISERGIAAI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ATIMRLLGLGRLPLSADRDKRAQFKGNVLTSDDRRYRRNLALIDARPQLRVGPPTARWLS
HHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHCEEEEECCCCCEECCCHHHHHH
EAFATMRRVLRHEHLTRITIPTVILAPTADRLVPYVPVEYLASNFRAGHLIPIDGARHEL
HHHHHHHHHHHHHHHHEEEECEEEECCCHHHCCCCCCHHHHHCCCCCCCEEECCCHHHHH
LQEADRYRAQAMAAIFAFLPGADAEDPGSLPRQLEEA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]