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The map label for this gene is gltD [H]

Identifier: 150397908

GI number: 150397908

Start: 2819477

End: 2820934

Strand: Reverse

Name: gltD [H]

Synonym: Smed_2710

Alternate gene names: 150397908

Gene position: 2820934-2819477 (Counterclockwise)

Preceding gene: 150397909

Following gene: 150397905

Centisome position: 74.59

GC content: 63.37

Gene sequence:

>1458_bases
ATGGGCAAGGTTACTGGGTTTTTGGAGATCGACCGGCAGGTAGCAAAGTACCAACCGGCATCTGACCGCATCCGCCATTT
CCGTGAATTCACAATTCCGATGTCCGATCAGGAAGTGCAGAAGCAATCCGCTCGCTGTATGGACTGCGGCATTCCCTATT
GCCACGGCCCGACCGGGTGCCCGGTGCACAACCAGATCCCGGATTGGAACGATCTCGTCTATAACGGCAACTGGGACGAG
GCGATCCGCAATCTGCATTCGACCAACAACTTCCCGGAATTCACCGGCAGAGTCTGCCCGGCGCCTTGCGAGGAAGCCTG
CACGCTGAACCTCGAAGACGTGCCGGTTGCGATCAAGACGGTCGAGCAGGCGATTGCCGATAAGGCCTACGAGATGGGCT
ATATCGTGCCCCAACCGGCCGTGACCAAGACCGGCAAGAAGGTCGCCGTCATCGGGTCCGGACCGGCCGGCATGGCGGCT
GCCCAGCAGCTGGCGCGCGCAGGCCACGAGGTGCATGTCTACGAACGAGAGTCCAAGCCTGGCGGGCTGCTGCGTTACGG
CATCCCGGATTTCAAAATGGAGAAGAACTTCATCGACCGCCGTGTCGACCAGATGCGGGGCGAAGGCGTGACGTTCCATT
GCGGCGTCAATGTCGGCGTGGAGGTGGCCATGCAGAAGCTCGTTGATGAAAACGACGCCGTGGTTTACTGCGGCGGCTCC
GAGACACCGCGCGACGCTGGCATTCCAGGCGTGGAGTTCGTCGGCGTGCACGACGCCATGCCTTATCTGGTGCAGCAGAA
CCGCCGGATCGGCCGCGAAAATATCGACAGCGTGGGCTGGCCGTCCGAGCCGATCCTGGCCGGCGGCAAGCACGTCGTCG
TGGTCGGCGGCGGAGACACGGCATCCGACTGCGTCGGAACCGCCTTCCGCCAGGGCGCCGTCAAGGTGACGCAGCTCGAC
ATTCGTCCGCAGCCGCCGGAAAAGGAGGACAAGCTCGCCGTCTGGCCCTTCTGGGCGACGAAGATGCGCACCTCCTCCAG
TCAGGCCGAGGGTGCGGTTCGCGAGTTCCAGGTTGCGACGCTCGAATTCGTCGGCGACGAGGACGGCAACCTGACCGGGG
TCAAATGCTGTCAGGTCGACGAGCGTCGCAAGCCGATCGCCGGTACCGAGTTCATCATCAAGGCCGATCTTGCCTTCATC
GCGATCGGTTTCCGTGGCCCCTTCGCAGATAGCGTGCTCAAGGACCTCGGCGACAAGCTGACGCTCAACACCGACCGGCG
CGGTTCGACCAATGTCGTTGCCGATGATCGCGACTACAGGACTTCGGTCGACAAGCTGTGGGCGGCGGGCGACATTCGCC
GCGGCCAGTCTCTTGTCGTCTGGGCGATCCGCGAAGGCCGGCAGGCGGCACGCGCGATCGACGAGGCGTTGATGGGCTCG
ACGGTGCTGCCGCGGTAG

Upstream 100 bases:

>100_bases
CGGCCCGTCGTGAAAACGGCTCCCGGCCCCTCGCCGGGTCAGAGGCCTTCACGCGCGCTTCAGGCGCGCCTGTTGAGATT
GAAGATATCAGGATCATAAG

Downstream 100 bases:

>100_bases
TTCAGGCGGGCGTCTCCCGACGCGCACCCTTTTTGGCCATTAGACTGTGCCTTCCTGCGACGCCCTCACCCGAGGATGAC
GGGCTTGAGGTGGGGCGCCG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDQEVQKQSARCMDCGIPYCHGPTGCPVHNQIPDWNDLVYNGNWDE
AIRNLHSTNNFPEFTGRVCPAPCEEACTLNLEDVPVAIKTVEQAIADKAYEMGYIVPQPAVTKTGKKVAVIGSGPAGMAA
AQQLARAGHEVHVYERESKPGGLLRYGIPDFKMEKNFIDRRVDQMRGEGVTFHCGVNVGVEVAMQKLVDENDAVVYCGGS
ETPRDAGIPGVEFVGVHDAMPYLVQQNRRIGRENIDSVGWPSEPILAGGKHVVVVGGGDTASDCVGTAFRQGAVKVTQLD
IRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAVREFQVATLEFVGDEDGNLTGVKCCQVDERRKPIAGTEFIIKADLAFI
AIGFRGPFADSVLKDLGDKLTLNTDRRGSTNVVADDRDYRTSVDKLWAAGDIRRGQSLVVWAIREGRQAARAIDEALMGS
TVLPR

Sequences:

>Translated_485_residues
MGKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDQEVQKQSARCMDCGIPYCHGPTGCPVHNQIPDWNDLVYNGNWDE
AIRNLHSTNNFPEFTGRVCPAPCEEACTLNLEDVPVAIKTVEQAIADKAYEMGYIVPQPAVTKTGKKVAVIGSGPAGMAA
AQQLARAGHEVHVYERESKPGGLLRYGIPDFKMEKNFIDRRVDQMRGEGVTFHCGVNVGVEVAMQKLVDENDAVVYCGGS
ETPRDAGIPGVEFVGVHDAMPYLVQQNRRIGRENIDSVGWPSEPILAGGKHVVVVGGGDTASDCVGTAFRQGAVKVTQLD
IRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAVREFQVATLEFVGDEDGNLTGVKCCQVDERRKPIAGTEFIIKADLAFI
AIGFRGPFADSVLKDLGDKLTLNTDRRGSTNVVADDRDYRTSVDKLWAAGDIRRGQSLVVWAIREGRQAARAIDEALMGS
TVLPR
>Mature_484_residues
GKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDQEVQKQSARCMDCGIPYCHGPTGCPVHNQIPDWNDLVYNGNWDEA
IRNLHSTNNFPEFTGRVCPAPCEEACTLNLEDVPVAIKTVEQAIADKAYEMGYIVPQPAVTKTGKKVAVIGSGPAGMAAA
QQLARAGHEVHVYERESKPGGLLRYGIPDFKMEKNFIDRRVDQMRGEGVTFHCGVNVGVEVAMQKLVDENDAVVYCGGSE
TPRDAGIPGVEFVGVHDAMPYLVQQNRRIGRENIDSVGWPSEPILAGGKHVVVVGGGDTASDCVGTAFRQGAVKVTQLDI
RPQPPEKEDKLAVWPFWATKMRTSSSQAEGAVREFQVATLEFVGDEDGNLTGVKCCQVDERRKPIAGTEFIIKADLAFIA
IGFRGPFADSVLKDLGDKLTLNTDRRGSTNVVADDRDYRTSVDKLWAAGDIRRGQSLVVWAIREGRQAARAIDEALMGST
VLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=486, Percent_Identity=25.7201646090535, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1789606, Length=488, Percent_Identity=36.6803278688525, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788811, Length=463, Percent_Identity=36.9330453563715, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI87082180, Length=478, Percent_Identity=34.7280334728033, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788468, Length=458, Percent_Identity=26.8558951965066, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1789243, Length=436, Percent_Identity=28.2110091743119, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17570289, Length=503, Percent_Identity=42.544731610338, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71984108, Length=305, Percent_Identity=27.2131147540984, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=172, Percent_Identity=33.7209302325581, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=497, Percent_Identity=40.8450704225352, Blast_Score=385, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28574881, Length=496, Percent_Identity=44.1532258064516, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24665539, Length=496, Percent_Identity=44.1532258064516, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24665547, Length=496, Percent_Identity=44.1532258064516, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24665543, Length=496, Percent_Identity=44.1532258064516, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24640763, Length=472, Percent_Identity=24.7881355932203, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18858217, Length=472, Percent_Identity=24.7881355932203, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53132; Mature: 53001

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDQEVQKQSARCMDCGIPYCHGPTGC
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
PVHNQIPDWNDLVYNGNWDEAIRNLHSTNNFPEFTGRVCPAPCEEACTLNLEDVPVAIKT
CCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHCCCCHHCCHHHHH
VEQAIADKAYEMGYIVPQPAVTKTGKKVAVIGSGPAGMAAAQQLARAGHEVHVYERESKP
HHHHHHHHHHHCCEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCC
GGLLRYGIPDFKMEKNFIDRRVDQMRGEGVTFHCGVNVGVEVAMQKLVDENDAVVYCGGS
CCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCC
ETPRDAGIPGVEFVGVHDAMPYLVQQNRRIGRENIDSVGWPSEPILAGGKHVVVVGGGDT
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEEEECCCC
ASDCVGTAFRQGAVKVTQLDIRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAVREFQVAT
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHEEEE
LEFVGDEDGNLTGVKCCQVDERRKPIAGTEFIIKADLAFIAIGFRGPFADSVLKDLGDKL
EEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEECEEEEEEECCCCCHHHHHHHCCCCE
TLNTDRRGSTNVVADDRDYRTSVDKLWAAGDIRRGQSLVVWAIREGRQAARAIDEALMGS
EECCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCC
TVLPR
CCCCC
>Mature Secondary Structure 
GKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDQEVQKQSARCMDCGIPYCHGPTGC
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
PVHNQIPDWNDLVYNGNWDEAIRNLHSTNNFPEFTGRVCPAPCEEACTLNLEDVPVAIKT
CCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHCCCCHHCCHHHHH
VEQAIADKAYEMGYIVPQPAVTKTGKKVAVIGSGPAGMAAAQQLARAGHEVHVYERESKP
HHHHHHHHHHHCCEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCC
GGLLRYGIPDFKMEKNFIDRRVDQMRGEGVTFHCGVNVGVEVAMQKLVDENDAVVYCGGS
CCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCC
ETPRDAGIPGVEFVGVHDAMPYLVQQNRRIGRENIDSVGWPSEPILAGGKHVVVVGGGDT
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEEEECCCC
ASDCVGTAFRQGAVKVTQLDIRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAVREFQVAT
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHEEEE
LEFVGDEDGNLTGVKCCQVDERRKPIAGTEFIIKADLAFIAIGFRGPFADSVLKDLGDKL
EEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEECEEEEEEECCCCCHHHHHHHCCCCE
TLNTDRRGSTNVVADDRDYRTSVDKLWAAGDIRRGQSLVVWAIREGRQAARAIDEALMGS
EECCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCC
TVLPR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]