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The map label for this gene is podJ [H]

Identifier: 150397864

GI number: 150397864

Start: 2771514

End: 2772659

Strand: Reverse

Name: podJ [H]

Synonym: Smed_2666

Alternate gene names: 150397864

Gene position: 2772659-2771514 (Counterclockwise)

Preceding gene: 150397865

Following gene: 150397863

Centisome position: 73.31

GC content: 63.79

Gene sequence:

>1146_bases
ATGGTAATCCGCTCCCTCCTGAATTCCAGCCGGCCCGTAGCCTTTGCCGCAGCGATGCTTTCAGCAGTCGCGCCGGCACT
GGCACAGCAACCGATACCGCCAGCGCAGACCGACGAGGGCAGTGCGCAGAAGCGCGGGCGGATCACGCCTTTCAACGGCG
CGGCACTACCGGAAGATGGCGAGCGAAAGCCGGCAGCGGCCGGGCCGGAAAAGCCGAAGCCCAGCGATGGCAGTGCGCCT
TCGAAGGGCGTCAACGTGATCGATCGCATGGGAGCAGAATTGCCTGATCTTCCTGAGGAGAAACCCTTCACAGGCAAGAT
CGACGAAGCTTATGGTGCCTTCCAGCGGGGCTATTACCTCACGGCGATGGACCTTGCCTTGCCGCGGGCCCAGCTCGGCG
ATCCAGCCGCCCAGACGCTCGTCGCGGGAATCCTCGAGCAGGGACTCGGTGTTGCGCGCGACGCCAAGGCCGCCGCCTTC
TGGTACGGCCAGGCGGCCACCAATGGCGATCCGGCGGCCATGTTCAAATATGCGCTGATCCTGATGGAAGGCCGCCACGT
CAAGCGCGACCGGAAGAAAGCAGACGAGCTCATGAAAAAGGCTGCCGATCTCGGCAATGCCTCGGCTCAGTTCAACTACG
GACAAACGCTGGTGGCCGACATGCCCGGCGAGCGCGGCCTGAAGGCCGCCATGCCCTATTACGAGAAATCGGCCGAACAA
GGCATTGCGGACGCGCAATATGCGCTGTCCCAGATCTACGTCAATGTCGACGGCGTCGAGGACGACAAACGCGCCCGCGC
CCGCGAGTGGCTGCTCAGGGCGGCGCGCGCGGGCTATGATACGGCGCAGCTCGACATTGCGATCTGGCTGATAGAAGGGA
TCGCCGGCGACCGCAACCTCGAAGAGGGCTTTGCCTGGATGAAGCGCGCCGCCGAAAGCGGCAACGTCGTCGCCCAGAAC
CGACTCTCCCACCTCTATGTGAATGCCATAGGTACGCGTCCGGACCCCGTCGAAGCGGCAAAATGGTACGTCCTGTCGCG
CCGGGCCGGCCTCAAAGATGACGCGCTCGAGGATTTCTATCTCGGCCTCAACGAAACGCAGCAGAAGTCGGCGCTCGCGG
CGGCCAACAAATACCGCTCGTCCTGA

Upstream 100 bases:

>100_bases
TGCCGTCTTCGGCGAAGCCGAACAGGCGCCGTCGGTGGTCGCGGCCGTCAACGCCTTGCTTGACGAAAAAGCGCCACGGT
TTGAAGGTTAAGACCTGCCG

Downstream 100 bases:

>100_bases
GATGCGTGGGCAGATCCGATCTATTTCTCTTGAACTTCCGGGGTTTTTGTGGTCTTGAAGCCCCGGTTTTCTCCGCGGCC
TCCTATTCCTATACCGCCGC

Product: Sel1 domain-containing protein

Products: NA

Alternate protein names: Polar organelle development protein [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MVIRSLLNSSRPVAFAAAMLSAVAPALAQQPIPPAQTDEGSAQKRGRITPFNGAALPEDGERKPAAAGPEKPKPSDGSAP
SKGVNVIDRMGAELPDLPEEKPFTGKIDEAYGAFQRGYYLTAMDLALPRAQLGDPAAQTLVAGILEQGLGVARDAKAAAF
WYGQAATNGDPAAMFKYALILMEGRHVKRDRKKADELMKKAADLGNASAQFNYGQTLVADMPGERGLKAAMPYYEKSAEQ
GIADAQYALSQIYVNVDGVEDDKRARAREWLLRAARAGYDTAQLDIAIWLIEGIAGDRNLEEGFAWMKRAAESGNVVAQN
RLSHLYVNAIGTRPDPVEAAKWYVLSRRAGLKDDALEDFYLGLNETQQKSALAAANKYRSS

Sequences:

>Translated_381_residues
MVIRSLLNSSRPVAFAAAMLSAVAPALAQQPIPPAQTDEGSAQKRGRITPFNGAALPEDGERKPAAAGPEKPKPSDGSAP
SKGVNVIDRMGAELPDLPEEKPFTGKIDEAYGAFQRGYYLTAMDLALPRAQLGDPAAQTLVAGILEQGLGVARDAKAAAF
WYGQAATNGDPAAMFKYALILMEGRHVKRDRKKADELMKKAADLGNASAQFNYGQTLVADMPGERGLKAAMPYYEKSAEQ
GIADAQYALSQIYVNVDGVEDDKRARAREWLLRAARAGYDTAQLDIAIWLIEGIAGDRNLEEGFAWMKRAAESGNVVAQN
RLSHLYVNAIGTRPDPVEAAKWYVLSRRAGLKDDALEDFYLGLNETQQKSALAAANKYRSS
>Mature_381_residues
MVIRSLLNSSRPVAFAAAMLSAVAPALAQQPIPPAQTDEGSAQKRGRITPFNGAALPEDGERKPAAAGPEKPKPSDGSAP
SKGVNVIDRMGAELPDLPEEKPFTGKIDEAYGAFQRGYYLTAMDLALPRAQLGDPAAQTLVAGILEQGLGVARDAKAAAF
WYGQAATNGDPAAMFKYALILMEGRHVKRDRKKADELMKKAADLGNASAQFNYGQTLVADMPGERGLKAAMPYYEKSAEQ
GIADAQYALSQIYVNVDGVEDDKRARAREWLLRAARAGYDTAQLDIAIWLIEGIAGDRNLEEGFAWMKRAAESGNVVAQN
RLSHLYVNAIGTRPDPVEAAKWYVLSRRAGLKDDALEDFYLGLNETQQKSALAAANKYRSS

Specific function: PodJL provides the positional information for the localization of several polar organelles (pili, adhesive holdfast and chemotactic apparatus) by recruiting structural (CpaE) and regulatory (PleC) proteins to a specific cell pole [H]

COG id: COG0790

COG function: function code R; FOG: TPR repeat, SEL1 subfamily

Gene ontology:

Cell location: Membrane; Single-pass membrane protein. Note=Both PodJL and podJS localize to the swarmer pole at different stages of the cell division [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 Sel1-like repeats [H]

Homologues:

Organism=Homo sapiens, GI154689719, Length=230, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87081769, Length=159, Percent_Identity=33.9622641509434, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002477
- InterPro:   IPR006597
- InterPro:   IPR011990 [H]

Pfam domain/function: PF01471 PG_binding_1; PF08238 Sel1 [H]

EC number: NA

Molecular weight: Translated: 41073; Mature: 41073

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIRSLLNSSRPVAFAAAMLSAVAPALAQQPIPPAQTDEGSAQKRGRITPFNGAALPEDG
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
ERKPAAAGPEKPKPSDGSAPSKGVNVIDRMGAELPDLPEEKPFTGKIDEAYGAFQRGYYL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEE
TAMDLALPRAQLGDPAAQTLVAGILEQGLGVARDAKAAAFWYGQAATNGDPAAMFKYALI
EEHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHEEECCCCCCCCHHHHHHHHHH
LMEGRHVKRDRKKADELMKKAADLGNASAQFNYGQTLVADMPGERGLKAAMPYYEKSAEQ
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHCCHHHHHHHC
GIADAQYALSQIYVNVDGVEDDKRARAREWLLRAARAGYDTAQLDIAIWLIEGIAGDRNL
CCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEECCCCCCCH
EEGFAWMKRAAESGNVVAQNRLSHLYVNAIGTRPDPVEAAKWYVLSRRAGLKDDALEDFY
HHHHHHHHHHHCCCCEEHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
LGLNETQQKSALAAANKYRSS
CCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVIRSLLNSSRPVAFAAAMLSAVAPALAQQPIPPAQTDEGSAQKRGRITPFNGAALPEDG
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
ERKPAAAGPEKPKPSDGSAPSKGVNVIDRMGAELPDLPEEKPFTGKIDEAYGAFQRGYYL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEE
TAMDLALPRAQLGDPAAQTLVAGILEQGLGVARDAKAAAFWYGQAATNGDPAAMFKYALI
EEHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHEEECCCCCCCCHHHHHHHHHH
LMEGRHVKRDRKKADELMKKAADLGNASAQFNYGQTLVADMPGERGLKAAMPYYEKSAEQ
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCHHHCCHHHHHHHC
GIADAQYALSQIYVNVDGVEDDKRARAREWLLRAARAGYDTAQLDIAIWLIEGIAGDRNL
CCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEEEEEEECCCCCCCH
EEGFAWMKRAAESGNVVAQNRLSHLYVNAIGTRPDPVEAAKWYVLSRRAGLKDDALEDFY
HHHHHHHHHHHCCCCEEHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
LGLNETQQKSALAAANKYRSS
CCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12370432 [H]