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The map label for this gene is pgk [H]

Identifier: 150397851

GI number: 150397851

Start: 2756802

End: 2758001

Strand: Direct

Name: pgk [H]

Synonym: Smed_2653

Alternate gene names: 150397851

Gene position: 2756802-2758001 (Clockwise)

Preceding gene: 150397850

Following gene: 150397852

Centisome position: 72.89

GC content: 64.5

Gene sequence:

>1200_bases
ATGACTTTCAAGACTCTCGACGATCTGACCGACATCGCCGGCAAGCGCGTCCTCGTGCGCGTCGACCTGAACGTGCCGGT
CAAGGACGGTCAGGTGACCGACACCACCCGCATCGAGCGCGTGGCGCCGACCATCCGCGAGCTCTCCGAAAAGGGTGCGA
AAGTCGTCCTGCTCGCACATTTCGGGCGGCCGAAGGGCGAACCGGTCGCCGACATGTCGCTGAAGACAATCGCCTCCGCC
GTCGAGGAGATCCTCGACCAGCGGGTCTATTTCGCGCCCGACTGCATCGGCGACAAAGCCGCCAATGCGATTGCCGATTT
GAATGACGGCGAAGTCCTGCTCCTCGAGAACACCCGATTCCACAAGGGCGAGGAGAAGAATGATCCGGCCTTCGTCACGG
CGCTCGCCGCCAATGGCGACATCTACGTCAACGACGCCTTCTCCGCCGCCCATCGCGCGCACGCCTCCACCGAGGGTCTG
GCCCAGCATCTTCCCGCCTATGCAGGCCGCACCATGCAGGCGGAGCTGGAAGCGCTGGAGAAGGGGCTCGGCAATCCGCA
GCGTCCAGTCGTCGCGATCGTCGGCGGCGCCAAGGTCTCTACCAAGATCGACCTTTTGCAGAATCTCGTGAAGAAGGTCG
ATGCGCTGGTCATCGGCGGCGGGATGGCCAACACCTTTCTCGCCGCGCAAGGCGTGGATGTCGGGAAGTCTCTCTGCGAG
CACGACCTTGCGGAAACCGCCAAGGCCATCCTCGCGGCCGCCTCGGAGGCGGGATGCGCGATCGTGCTTCCGGTGGACGG
GGTTGTCGCCCGTGAATTCAAGGCCGGCGCCGACAATGAGGTCGTCGACGTCAAGGCCATCCCTGCCGACGCCATGATGC
TCGACGTCGGTCCGAAGTCGATCGACGCGATCAACGAATGGATCTCGCGCGCCGAAACCCTCGTATGGAACGGCCCGCTC
GGCGCCTTCGAGATCACACCCTTCGACAAGGCGACCGTGGCGGCGGCGAAACATGCGGCAGCCCGCACGCGCTCCGGCTC
GCTCGTTTCGGTCGCCGGTGGCGGCGATACCGTTGCGGCACTCAATCATGCCGAGGTGGCTGACGACTTTACCTATGTGT
CGACAGCCGGCGGCGCCTTCCTCGAATGGATGGAGGGCAAGCCCCTGCCGGGCGTCGATATTCTCAAGCAGCAGAGGTGA

Upstream 100 bases:

>100_bases
CGGCGGATCGCAGCTTCACGGCCGAGCTGCCGGTGGACGAGCACGGCCTCGTCATCGACTACCCTACCCTGTTCCAGCGA
CTATCACCGGAGACCATCTG

Downstream 100 bases:

>100_bases
CGCTCAGGGGAAGCCGCCCTTGTGCACGGCTCCGCATCAGAGGATGCGGAGCGCCTCACGACCAACTCGAAACGCAAGCC
GCTGAGAAGCTCGCGACGCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTFKTLDDLTDIAGKRVLVRVDLNVPVKDGQVTDTTRIERVAPTIRELSEKGAKVVLLAHFGRPKGEPVADMSLKTIASA
VEEILDQRVYFAPDCIGDKAANAIADLNDGEVLLLENTRFHKGEEKNDPAFVTALAANGDIYVNDAFSAAHRAHASTEGL
AQHLPAYAGRTMQAELEALEKGLGNPQRPVVAIVGGAKVSTKIDLLQNLVKKVDALVIGGGMANTFLAAQGVDVGKSLCE
HDLAETAKAILAAASEAGCAIVLPVDGVVAREFKAGADNEVVDVKAIPADAMMLDVGPKSIDAINEWISRAETLVWNGPL
GAFEITPFDKATVAAAKHAAARTRSGSLVSVAGGGDTVAALNHAEVADDFTYVSTAGGAFLEWMEGKPLPGVDILKQQR

Sequences:

>Translated_399_residues
MTFKTLDDLTDIAGKRVLVRVDLNVPVKDGQVTDTTRIERVAPTIRELSEKGAKVVLLAHFGRPKGEPVADMSLKTIASA
VEEILDQRVYFAPDCIGDKAANAIADLNDGEVLLLENTRFHKGEEKNDPAFVTALAANGDIYVNDAFSAAHRAHASTEGL
AQHLPAYAGRTMQAELEALEKGLGNPQRPVVAIVGGAKVSTKIDLLQNLVKKVDALVIGGGMANTFLAAQGVDVGKSLCE
HDLAETAKAILAAASEAGCAIVLPVDGVVAREFKAGADNEVVDVKAIPADAMMLDVGPKSIDAINEWISRAETLVWNGPL
GAFEITPFDKATVAAAKHAAARTRSGSLVSVAGGGDTVAALNHAEVADDFTYVSTAGGAFLEWMEGKPLPGVDILKQQR
>Mature_398_residues
TFKTLDDLTDIAGKRVLVRVDLNVPVKDGQVTDTTRIERVAPTIRELSEKGAKVVLLAHFGRPKGEPVADMSLKTIASAV
EEILDQRVYFAPDCIGDKAANAIADLNDGEVLLLENTRFHKGEEKNDPAFVTALAANGDIYVNDAFSAAHRAHASTEGLA
QHLPAYAGRTMQAELEALEKGLGNPQRPVVAIVGGAKVSTKIDLLQNLVKKVDALVIGGGMANTFLAAQGVDVGKSLCEH
DLAETAKAILAAASEAGCAIVLPVDGVVAREFKAGADNEVVDVKAIPADAMMLDVGPKSIDAINEWISRAETLVWNGPLG
AFEITPFDKATVAAAKHAAARTRSGSLVSVAGGGDTVAALNHAEVADDFTYVSTAGGAFLEWMEGKPLPGVDILKQQR

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=42.8223844282238, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=42.3357664233577, Blast_Score=282, Evalue=5e-76,
Organism=Escherichia coli, GI1789294, Length=400, Percent_Identity=44.75, Blast_Score=304, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI17508823, Length=406, Percent_Identity=44.0886699507389, Blast_Score=304, Evalue=5e-83,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=39.225181598063, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24581203, Length=401, Percent_Identity=42.14463840399, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=40.4358353510896, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41931; Mature: 41800

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKTLDDLTDIAGKRVLVRVDLNVPVKDGQVTDTTRIERVAPTIRELSEKGAKVVLLAH
CCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
FGRPKGEPVADMSLKTIASAVEEILDQRVYFAPDCIGDKAANAIADLNDGEVLLLENTRF
CCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHCCCCCCEEEEECCCC
HKGEEKNDPAFVTALAANGDIYVNDAFSAAHRAHASTEGLAQHLPAYAGRTMQAELEALE
CCCCCCCCCEEEEEEECCCCEEEECHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
KGLGNPQRPVVAIVGGAKVSTKIDLLQNLVKKVDALVIGGGMANTFLAAQGVDVGKSLCE
HHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCCHHHHHHHH
HDLAETAKAILAAASEAGCAIVLPVDGVVAREFKAGADNEVVDVKAIPADAMMLDVGPKS
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCEEEEEECCCCEEEEECCCHH
IDAINEWISRAETLVWNGPLGAFEITPFDKATVAAAKHAAARTRSGSLVSVAGGGDTVAA
HHHHHHHHHHHCEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEE
LNHAEVADDFTYVSTAGGAFLEWMEGKPLPGVDILKQQR
ECCHHHHCCCEEEECCCCHHHHHHCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TFKTLDDLTDIAGKRVLVRVDLNVPVKDGQVTDTTRIERVAPTIRELSEKGAKVVLLAH
CCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
FGRPKGEPVADMSLKTIASAVEEILDQRVYFAPDCIGDKAANAIADLNDGEVLLLENTRF
CCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHCCCCCCEEEEECCCC
HKGEEKNDPAFVTALAANGDIYVNDAFSAAHRAHASTEGLAQHLPAYAGRTMQAELEALE
CCCCCCCCCEEEEEEECCCCEEEECHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
KGLGNPQRPVVAIVGGAKVSTKIDLLQNLVKKVDALVIGGGMANTFLAAQGVDVGKSLCE
HHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCCHHHHHHHH
HDLAETAKAILAAASEAGCAIVLPVDGVVAREFKAGADNEVVDVKAIPADAMMLDVGPKS
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCEEEEEECCCCEEEEECCCHH
IDAINEWISRAETLVWNGPLGAFEITPFDKATVAAAKHAAARTRSGSLVSVAGGGDTVAA
HHHHHHHHHHHCEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEE
LNHAEVADDFTYVSTAGGAFLEWMEGKPLPGVDILKQQR
ECCHHHHCCCEEEECCCCHHHHHHCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]