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The map label for this gene is rpoH [H]

Identifier: 150397812

GI number: 150397812

Start: 2709345

End: 2710250

Strand: Direct

Name: rpoH [H]

Synonym: Smed_2614

Alternate gene names: 150397812

Gene position: 2709345-2710250 (Clockwise)

Preceding gene: 150397811

Following gene: 150397815

Centisome position: 71.64

GC content: 59.27

Gene sequence:

>906_bases
ATGGCCCGCAATACCTTGCCGACCATTGCCGCTGGAGAGGGCGGCCTCAATCGCTATCTCGACGAGATTCGCAAATTTCC
CATGCTCGAGCCGCAGGAAGAGTACATGCTCGCCAAGCGGTACCAGGAGCATGATGATCGTAAGGCCGCGCACAAGCTCG
TAACGAGCCATCTTCGCCTCGTCGCCAAGATCGCGATGGGCTATCGCGGCTACGGCCTGCCAATCGGTGAAGTCATCTCT
GAAGGCAATGTCGGCCTGATGCAGGCTGTCAAGAAGTTCGAACCCGATCGCGGCTTTCGGCTCGCGACCTACGCCATGTG
GTGGATCAAGGCTGCGATACAGGAATATATCCTGCGCTCATGGTCCCTCGTCAAAATGGGCACAACAGCCAACCAGAAAC
GCCTTTTCTTCAACCTCAGACGGCTGAAAGGCCGTATCCAGGCGCTCGATGAAGGCGATCTCAAGCCGGAACAGGTGAAG
GAGATCGCCACGACCTTGAAGGTCAGCGAGGACGAGGTCATTTCGATGAACCGCCGCCTGTCGGGCGACGCATCGCTCAA
TGCGCCGATCAAGGCGAGCGAAGGCGATTCCGGCCAGTGGCAGGACTGGTTGGTCGACGAAAACGACAACCAGGAACAGA
TCCTGATCGAACAGGACGAGCTCGACAATCGTCGCGCGCTTCTGGCCAATGCCTTGAAGGTTCTGAACGACCGTGAGCGC
CGGATTTTCGAAGCTCGGCGCCTGACCGAGGATCCGATAACGCTGGAGGACCTCTCGACCGAGTTCGACATCAGCCGCGA
GCGCGTGCGCCAGATCGAGGTTCGTGCGTTCGAGAAGGTCCAGGAAGCTGTGCGTAAGGCTGCGCTGGAACGCGCCAATG
CGTTGCGCGTGGTGGAAGGCGCGTAG

Upstream 100 bases:

>100_bases
TTTCGCCGCACTTATCTATTAGCATCGTGGGGTCTTGGATGAAGACCCACAGGCCCGGCCTGCCCTCCCGGAGGCCGGCG
ACGCACAAAGGGGGTGCTTC

Downstream 100 bases:

>100_bases
AACGCTTCCGGCATTTGCACACCAAACGAAAATCCCGGCTGGAGCCCCAGCCGGGATTTTCGTTTCAGCTCATGCGGCAC
GCCTTCATTGCCCGCTCGAA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MARNTLPTIAAGEGGLNRYLDEIRKFPMLEPQEEYMLAKRYQEHDDRKAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVIS
EGNVGLMQAVKKFEPDRGFRLATYAMWWIKAAIQEYILRSWSLVKMGTTANQKRLFFNLRRLKGRIQALDEGDLKPEQVK
EIATTLKVSEDEVISMNRRLSGDASLNAPIKASEGDSGQWQDWLVDENDNQEQILIEQDELDNRRALLANALKVLNDRER
RIFEARRLTEDPITLEDLSTEFDISRERVRQIEVRAFEKVQEAVRKAALERANALRVVEGA

Sequences:

>Translated_301_residues
MARNTLPTIAAGEGGLNRYLDEIRKFPMLEPQEEYMLAKRYQEHDDRKAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVIS
EGNVGLMQAVKKFEPDRGFRLATYAMWWIKAAIQEYILRSWSLVKMGTTANQKRLFFNLRRLKGRIQALDEGDLKPEQVK
EIATTLKVSEDEVISMNRRLSGDASLNAPIKASEGDSGQWQDWLVDENDNQEQILIEQDELDNRRALLANALKVLNDRER
RIFEARRLTEDPITLEDLSTEFDISRERVRQIEVRAFEKVQEAVRKAALERANALRVVEGA
>Mature_300_residues
ARNTLPTIAAGEGGLNRYLDEIRKFPMLEPQEEYMLAKRYQEHDDRKAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVISE
GNVGLMQAVKKFEPDRGFRLATYAMWWIKAAIQEYILRSWSLVKMGTTANQKRLFFNLRRLKGRIQALDEGDLKPEQVKE
IATTLKVSEDEVISMNRRLSGDASLNAPIKASEGDSGQWQDWLVDENDNQEQILIEQDELDNRRALLANALKVLNDRERR
IFEARRLTEDPITLEDLSTEFDISRERVRQIEVRAFEKVQEAVRKAALERANALRVVEGA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=37.8947368421053, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789098, Length=275, Percent_Identity=34.1818181818182, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789448, Length=250, Percent_Identity=31.6, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34567; Mature: 34436

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNTLPTIAAGEGGLNRYLDEIRKFPMLEPQEEYMLAKRYQEHDDRKAAHKLVTSHLRL
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFEPDRGFRLATYAMWWIKAAIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKRLFFNLRRLKGRIQALDEGDLKPEQVKEIATTLKVSEDEVISMNRRL
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
SGDASLNAPIKASEGDSGQWQDWLVDENDNQEQILIEQDELDNRRALLANALKVLNDRER
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
RIFEARRLTEDPITLEDLSTEFDISRERVRQIEVRAFEKVQEAVRKAALERANALRVVEG
HHHHHHHCCCCCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
A
C
>Mature Secondary Structure 
ARNTLPTIAAGEGGLNRYLDEIRKFPMLEPQEEYMLAKRYQEHDDRKAAHKLVTSHLRL
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKIAMGYRGYGLPIGEVISEGNVGLMQAVKKFEPDRGFRLATYAMWWIKAAIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKRLFFNLRRLKGRIQALDEGDLKPEQVKEIATTLKVSEDEVISMNRRL
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
SGDASLNAPIKASEGDSGQWQDWLVDENDNQEQILIEQDELDNRRALLANALKVLNDRER
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
RIFEARRLTEDPITLEDLSTEFDISRERVRQIEVRAFEKVQEAVRKAALERANALRVVEG
HHHHHHHCCCCCCCHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]