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The map label for this gene is hipO [H]

Identifier: 150397771

GI number: 150397771

Start: 2670146

End: 2671309

Strand: Direct

Name: hipO [H]

Synonym: Smed_2573

Alternate gene names: 150397771

Gene position: 2670146-2671309 (Clockwise)

Preceding gene: 150397768

Following gene: 150397772

Centisome position: 70.6

GC content: 64.0

Gene sequence:

>1164_bases
ATGCCGATTTTGAACCGTGCCGCCGACCTCAAGACCGAAGTGACAGAATGGCGGCGCCACCTGCACCAGAACCCGGAGCT
TCTGTTCGCAGTCGAAAACACGGCAGCCTTCGTCGAACGGAAACTGAAGGAATTCGGCGTTGACGAAATCGTGACCGGGC
TCGGCCGCACCGGAGTGGTCGGCCTGATCCGTGGCAATCGCGGCCCCGGCCGCACCATCGGGCTCAGGGCCGACATGGAT
GCTCTGCCGATCACTGAGGCGAGCGGAATGGCCTGGACTTCGAAGACCCCCGGCAAAATGCATGCCTGCGGCCACGACGG
GCACACGGCCATGCTGCTTGGCGCGGCGAAATACCTGGCTGAAACGCGGAATTTTGCCGGCAATGTCGCCGTGATCTTCC
AGCCGGCCGAGGAAGGCGGCGGCGGCGGAAACGAGATGGTGAAGGACGGCATGATGGAGCGCTTCGCGATCGAGGAAGTC
TATGGCATGCACAACATGCCGGGCATGCCGGTCGGCCATTTCGGCAGCCGTGTCGGTCCGATCATGGCCTCAACCGACGA
ATTCACCATCACGGTCAAGGGCCGCGGCGGACACGCGGCTCAGCCGCACAGGACGATCGACCCGATCGCCATCGGTGCCC
AGATCGTCAACGCGCTTCAGACGATCGCCTCGCGCACCGTCGATCCGCTTGCCTCGATCGTCGTCTCCGTCACCAAGTTC
AATGCGGGCTTCGCGCACAACGTCATCCCCGAACAGGCCACTCTCGCCGGCACAGTGCGCGCTTTGACGCCGGAGGTGCG
GGACATCGGCGAAGTCCGGATCCGCCAGATCGCCGAGAGCATCGCTCTCGCCTATGGCGCCACGGTCGACATCTGGTACG
GCCGTAACTATCCGGTGACCGTGAACCACGCCGCCGAAACCGGCCACGCGCTTGCCGTCGCCGCCACGGTTGCCGGCGAA
GGCAACGTCGATGAAGCGCTCGATCCGTTGATGGGCGGCGAGGACTTCTCCTACATGCTGCTTTCCCGGCCGGGCGCCTT
CGTGTTCATCGGAAATGGCGACACGGCGGGCCTGCATCATCCGGCCTATGACTTCAACGACGAAGTCATCCCGCATGGAA
TCTCCTATTGGGTGAAACTCGCCGAGGCACGGCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CCGCCGGAACACTCCGGCGGCGAGACCTCAGCCGGACGCTCCAGCGCCGGACCTCAGGTTTCGAACCCCATGGAACGAAC
AGACCGACAGGAAACGAACG

Downstream 100 bases:

>100_bases
ATAACGGAAAGCGGGAGGGGGCCATGCACCAGATCGACAAGACGGACCGGAGAATCCTCAATATCCTGCAGGCGGACGGG
CGGATTACCAATCTGGAACT

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPILNRAADLKTEVTEWRRHLHQNPELLFAVENTAAFVERKLKEFGVDEIVTGLGRTGVVGLIRGNRGPGRTIGLRADMD
ALPITEASGMAWTSKTPGKMHACGHDGHTAMLLGAAKYLAETRNFAGNVAVIFQPAEEGGGGGNEMVKDGMMERFAIEEV
YGMHNMPGMPVGHFGSRVGPIMASTDEFTITVKGRGGHAAQPHRTIDPIAIGAQIVNALQTIASRTVDPLASIVVSVTKF
NAGFAHNVIPEQATLAGTVRALTPEVRDIGEVRIRQIAESIALAYGATVDIWYGRNYPVTVNHAAETGHALAVAATVAGE
GNVDEALDPLMGGEDFSYMLLSRPGAFVFIGNGDTAGLHHPAYDFNDEVIPHGISYWVKLAEARLAA

Sequences:

>Translated_387_residues
MPILNRAADLKTEVTEWRRHLHQNPELLFAVENTAAFVERKLKEFGVDEIVTGLGRTGVVGLIRGNRGPGRTIGLRADMD
ALPITEASGMAWTSKTPGKMHACGHDGHTAMLLGAAKYLAETRNFAGNVAVIFQPAEEGGGGGNEMVKDGMMERFAIEEV
YGMHNMPGMPVGHFGSRVGPIMASTDEFTITVKGRGGHAAQPHRTIDPIAIGAQIVNALQTIASRTVDPLASIVVSVTKF
NAGFAHNVIPEQATLAGTVRALTPEVRDIGEVRIRQIAESIALAYGATVDIWYGRNYPVTVNHAAETGHALAVAATVAGE
GNVDEALDPLMGGEDFSYMLLSRPGAFVFIGNGDTAGLHHPAYDFNDEVIPHGISYWVKLAEARLAA
>Mature_386_residues
PILNRAADLKTEVTEWRRHLHQNPELLFAVENTAAFVERKLKEFGVDEIVTGLGRTGVVGLIRGNRGPGRTIGLRADMDA
LPITEASGMAWTSKTPGKMHACGHDGHTAMLLGAAKYLAETRNFAGNVAVIFQPAEEGGGGGNEMVKDGMMERFAIEEVY
GMHNMPGMPVGHFGSRVGPIMASTDEFTITVKGRGGHAAQPHRTIDPIAIGAQIVNALQTIASRTVDPLASIVVSVTKFN
AGFAHNVIPEQATLAGTVRALTPEVRDIGEVRIRQIAESIALAYGATVDIWYGRNYPVTVNHAAETGHALAVAATVAGEG
NVDEALDPLMGGEDFSYMLLSRPGAFVFIGNGDTAGLHHPAYDFNDEVIPHGISYWVKLAEARLAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=423, Percent_Identity=27.1867612293144, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41264; Mature: 41132

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILNRAADLKTEVTEWRRHLHQNPELLFAVENTAAFVERKLKEFGVDEIVTGLGRTGVV
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCHHHHHHHCCCCCEE
GLIRGNRGPGRTIGLRADMDALPITEASGMAWTSKTPGKMHACGHDGHTAMLLGAAKYLA
EEEECCCCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCEEECCCCCCHHHHHHHHHHHH
ETRNFAGNVAVIFQPAEEGGGGGNEMVKDGMMERFAIEEVYGMHNMPGMPVGHFGSRVGP
HHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
IMASTDEFTITVKGRGGHAAQPHRTIDPIAIGAQIVNALQTIASRTVDPLASIVVSVTKF
CEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NAGFAHNVIPEQATLAGTVRALTPEVRDIGEVRIRQIAESIALAYGATVDIWYGRNYPVT
CCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEE
VNHAAETGHALAVAATVAGEGNVDEALDPLMGGEDFSYMLLSRPGAFVFIGNGDTAGLHH
EECCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCC
PAYDFNDEVIPHGISYWVKLAEARLAA
CCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PILNRAADLKTEVTEWRRHLHQNPELLFAVENTAAFVERKLKEFGVDEIVTGLGRTGVV
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCHHHHHHHCCCCCEE
GLIRGNRGPGRTIGLRADMDALPITEASGMAWTSKTPGKMHACGHDGHTAMLLGAAKYLA
EEEECCCCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCEEECCCCCCHHHHHHHHHHHH
ETRNFAGNVAVIFQPAEEGGGGGNEMVKDGMMERFAIEEVYGMHNMPGMPVGHFGSRVGP
HHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
IMASTDEFTITVKGRGGHAAQPHRTIDPIAIGAQIVNALQTIASRTVDPLASIVVSVTKF
CEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NAGFAHNVIPEQATLAGTVRALTPEVRDIGEVRIRQIAESIALAYGATVDIWYGRNYPVT
CCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEE
VNHAAETGHALAVAATVAGEGNVDEALDPLMGGEDFSYMLLSRPGAFVFIGNGDTAGLHH
EECCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCC
PAYDFNDEVIPHGISYWVKLAEARLAA
CCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]