Click here to switch to the map view.

The map label for this gene is cobT [H]

Identifier: 150397757

GI number: 150397757

Start: 2656021

End: 2657916

Strand: Reverse

Name: cobT [H]

Synonym: Smed_2559

Alternate gene names: 150397757

Gene position: 2657916-2656021 (Counterclockwise)

Preceding gene: 150397758

Following gene: 150397756

Centisome position: 70.28

GC content: 63.77

Gene sequence:

>1896_bases
GTGAGTTCGAATTCCAAGGCGAAACCGAATACGAGGGAAAACGCCGCCGAACCGTTCAAGCGGGCGCTCTCCGGCTGCGT
CAGGTCCATCGCCGGCGATGCGGAGCTTGAGGTCGCTTTTGCCAATGAGCGGCCGGGAATGACAGCCGAGCGCATTCGGC
TGCCGGAACTCTCCAAACGCCCGACCCGTCAGGAGCTGGCAGTGGCGCGGGGCCTTGGCGATTCCATGGCGCTGCGCAAG
GCCTGCCACGATGCTCGAGTCCATGCGACGATGTCGCCGCAGGGGGCGGACGCCCGAGCGATCTTCGATGCGGTCGAGCA
GGCGCGCGTCGAGGCGATCGGCGCTCTCAGGATGCCGGGTGTGGCCGACAATCTCTCCTCCATGCTCCAGGAGAAATACG
CCAAGGCGAATTTCTCGGCAATCAATGCCCAGGCGGATGCGCCGCTCGAGGAGGCGGTGGCGCTGCTCGTGCGCGAGAAG
CTGACCGGCGAGAAGCCGCCGGAATCGGCCGGCCAGGTCCTCGACCTCTGGCGTGAGTTCATTGAGCAGAAGGCCGGCAG
CGACATGCGCAACCTTGCCGGTACGGTCAACGATCAGCAGGCTTTTGCCCGCGTCGTGCGCGACATGCTGTCTTCGATGG
ACGTTGCCGAGAAATACGGCGACGACGATAGCGAGCCGGACGAACAGGAGAGCGAGACCGACGAGGACCAGCCGCGCAGT
CAGGAGCAGGACGAAAACGCCAGCGACGAAGAGCAGGGCTCCGATGCCGCCCCTGCCGAGGAAAACCAGTCTGCCGAGGA
GCAGATGGAAGATGGCGAAATGGACGGCGCGGAAATTTCCGACGACGACCTTCAGGACGAAGGCGACGAGGACAGCGAAA
CGCCGGGCGAGGTCAAGCGGCCGAACCATCCCTTCGCGGATTTCAACGAGAAGGTGGATTATTCCGTCTATACCCGGGAT
TTCGACGAAACGATTGCCTCGGAAGAGCTCTGCGACGAGGCGGAGCTCGACAGGCTGCGCGCTTTTCTCGACAAGCAGCT
CGCCCATCTGCAGGGTGCAGTGGGGCGCCTCGCCAATCGCCTGCAGCGCCGCCTGATGGCGCAGCAGAACCGGTCCTGGG
AGTTCGATCTCGAGGAGGGGTATCTCGACACGGCCCGGCTGCAGCGCATCATCATCGATCCTATGCAGCCGCTTTCCTTC
AAGAGGGAGAAGGATACCAATTTCCGCGACACGGTCGTCACGCTGCTGATAGACAATTCCGGCTCCATGCGCGGGCGTCC
GATCACGGTAGCAGCCACCTGCGCCGACATTCTTGCGCGAACGCTCGAGCGTTGCGGCGTGAAGGTCGAAATCCTCGGAT
TCACGACAAAGGCCTGGAAGGGCGGACAGTCGCGCGAGAAATGGCTTGCCGGCGGCAAGCCCCAGTCGCCGGGCCGCCTC
AACGACCTCAGGCACATCATCTATAAGTCCGCTGACGCGCCATGGCGCCGCGCACGGCGCAATCTCGGTCTGATGATGCG
CGAGGGACTGCTCAAGGAGAACATCGACGGCGAGGCGCTGATGTGGGCGCATGACCGGCTCCTGGCACGGTCCGAGCAAA
GACGCATTCTCATGATGATCTCGGATGGCGCGCCGGTCGACGATTCGACCCTTTCGGTGAACCCCGGAAATTACCTCGAA
CGCCATCTGCGCGCCGTTATCGAGCAGATCGAAACGCGCTCTCCGGTCGAGTTGCTTGCGATCGGCATCGGGCACGACGT
CACACGCTATTATCGCCGCGCCGTGACGATCGTCGATGCGGACGAACTCGCGGGAGCGATGACGGAGCAGCTTGCAGCCC
TCTTCGAGGACGAGAGTACGCGCCGCCGCCCGGGCGGGGCGCGTCGAGCCGGTTAA

Upstream 100 bases:

>100_bases
CCCTGGAGCGGGAGGGATTGTTTTTTATGCAGATTCCGCTCCTATCATTATAGTCGATGTGCTCGGATAGCGTCCGAATC
CGGCTGGAGAAGGAATTGCA

Downstream 100 bases:

>100_bases
TCAAGCCGGCCGCGCCGGATTCCCCCTCGACGGGTGCCGCTCCGGTCCGGCAGCGGCTGCCCCGCGTAAGGTCATTGCTC
AGATGTTCTGCCGACATTTC

Product: cobalt chelatase, pCobT subunit

Products: NA

Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobT [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSSNSKAKPNTRENAAEPFKRALSGCVRSIAGDAELEVAFANERPGMTAERIRLPELSKRPTRQELAVARGLGDSMALRK
ACHDARVHATMSPQGADARAIFDAVEQARVEAIGALRMPGVADNLSSMLQEKYAKANFSAINAQADAPLEEAVALLVREK
LTGEKPPESAGQVLDLWREFIEQKAGSDMRNLAGTVNDQQAFARVVRDMLSSMDVAEKYGDDDSEPDEQESETDEDQPRS
QEQDENASDEEQGSDAAPAEENQSAEEQMEDGEMDGAEISDDDLQDEGDEDSETPGEVKRPNHPFADFNEKVDYSVYTRD
FDETIASEELCDEAELDRLRAFLDKQLAHLQGAVGRLANRLQRRLMAQQNRSWEFDLEEGYLDTARLQRIIIDPMQPLSF
KREKDTNFRDTVVTLLIDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTKAWKGGQSREKWLAGGKPQSPGRL
NDLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALMWAHDRLLARSEQRRILMMISDGAPVDDSTLSVNPGNYLE
RHLRAVIEQIETRSPVELLAIGIGHDVTRYYRRAVTIVDADELAGAMTEQLAALFEDESTRRRPGGARRAG

Sequences:

>Translated_631_residues
MSSNSKAKPNTRENAAEPFKRALSGCVRSIAGDAELEVAFANERPGMTAERIRLPELSKRPTRQELAVARGLGDSMALRK
ACHDARVHATMSPQGADARAIFDAVEQARVEAIGALRMPGVADNLSSMLQEKYAKANFSAINAQADAPLEEAVALLVREK
LTGEKPPESAGQVLDLWREFIEQKAGSDMRNLAGTVNDQQAFARVVRDMLSSMDVAEKYGDDDSEPDEQESETDEDQPRS
QEQDENASDEEQGSDAAPAEENQSAEEQMEDGEMDGAEISDDDLQDEGDEDSETPGEVKRPNHPFADFNEKVDYSVYTRD
FDETIASEELCDEAELDRLRAFLDKQLAHLQGAVGRLANRLQRRLMAQQNRSWEFDLEEGYLDTARLQRIIIDPMQPLSF
KREKDTNFRDTVVTLLIDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTKAWKGGQSREKWLAGGKPQSPGRL
NDLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALMWAHDRLLARSEQRRILMMISDGAPVDDSTLSVNPGNYLE
RHLRAVIEQIETRSPVELLAIGIGHDVTRYYRRAVTIVDADELAGAMTEQLAALFEDESTRRRPGGARRAG
>Mature_630_residues
SSNSKAKPNTRENAAEPFKRALSGCVRSIAGDAELEVAFANERPGMTAERIRLPELSKRPTRQELAVARGLGDSMALRKA
CHDARVHATMSPQGADARAIFDAVEQARVEAIGALRMPGVADNLSSMLQEKYAKANFSAINAQADAPLEEAVALLVREKL
TGEKPPESAGQVLDLWREFIEQKAGSDMRNLAGTVNDQQAFARVVRDMLSSMDVAEKYGDDDSEPDEQESETDEDQPRSQ
EQDENASDEEQGSDAAPAEENQSAEEQMEDGEMDGAEISDDDLQDEGDEDSETPGEVKRPNHPFADFNEKVDYSVYTRDF
DETIASEELCDEAELDRLRAFLDKQLAHLQGAVGRLANRLQRRLMAQQNRSWEFDLEEGYLDTARLQRIIIDPMQPLSFK
REKDTNFRDTVVTLLIDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTKAWKGGQSREKWLAGGKPQSPGRLN
DLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALMWAHDRLLARSEQRRILMMISDGAPVDDSTLSVNPGNYLER
HLRAVIEQIETRSPVELLAIGIGHDVTRYYRRAVTIVDADELAGAMTEQLAALFEDESTRRRPGGARRAG

Specific function: Catalyzes cobalt insertion in the corrin ring [H]

COG id: COG4547

COG function: function code H; Cobalamin biosynthesis protein CobT (nicotinate-mononucleotide:5, 6-dimethylbenzimidazole phosphoribosyltransferase)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006538
- InterPro:   IPR002035 [H]

Pfam domain/function: PF06213 CobT [H]

EC number: =6.6.1.2 [H]

Molecular weight: Translated: 70244; Mature: 70113

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50234 VWFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNSKAKPNTRENAAEPFKRALSGCVRSIAGDAELEVAFANERPGMTAERIRLPELSKR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCCCCCC
PTRQELAVARGLGDSMALRKACHDARVHATMSPQGADARAIFDAVEQARVEAIGALRMPG
CCHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
VADNLSSMLQEKYAKANFSAINAQADAPLEEAVALLVREKLTGEKPPESAGQVLDLWREF
HHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IEQKAGSDMRNLAGTVNDQQAFARVVRDMLSSMDVAEKYGDDDSEPDEQESETDEDQPRS
HHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCC
QEQDENASDEEQGSDAAPAEENQSAEEQMEDGEMDGAEISDDDLQDEGDEDSETPGEVKR
HHHCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PNHPFADFNEKVDYSVYTRDFDETIASEELCDEAELDRLRAFLDKQLAHLQGAVGRLANR
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRRLMAQQNRSWEFDLEEGYLDTARLQRIIIDPMQPLSFKREKDTNFRDTVVTLLIDNS
HHHHHHHHHCCCCEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEECC
GSMRGRPITVAATCADILARTLERCGVKVEILGFTTKAWKGGQSREKWLAGGKPQSPGRL
CCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCHHHHHCCCCCCCCCHH
NDLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALMWAHDRLLARSEQRRILMMI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCEEEEEE
SDGAPVDDSTLSVNPGNYLERHLRAVIEQIETRSPVELLAIGIGHDVTRYYRRAVTIVDA
ECCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHEECH
DELAGAMTEQLAALFEDESTRRRPGGARRAG
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
>Mature Secondary Structure 
SSNSKAKPNTRENAAEPFKRALSGCVRSIAGDAELEVAFANERPGMTAERIRLPELSKR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCCCCCCCC
PTRQELAVARGLGDSMALRKACHDARVHATMSPQGADARAIFDAVEQARVEAIGALRMPG
CCHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
VADNLSSMLQEKYAKANFSAINAQADAPLEEAVALLVREKLTGEKPPESAGQVLDLWREF
HHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
IEQKAGSDMRNLAGTVNDQQAFARVVRDMLSSMDVAEKYGDDDSEPDEQESETDEDQPRS
HHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCC
QEQDENASDEEQGSDAAPAEENQSAEEQMEDGEMDGAEISDDDLQDEGDEDSETPGEVKR
HHHCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PNHPFADFNEKVDYSVYTRDFDETIASEELCDEAELDRLRAFLDKQLAHLQGAVGRLANR
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRRLMAQQNRSWEFDLEEGYLDTARLQRIIIDPMQPLSFKREKDTNFRDTVVTLLIDNS
HHHHHHHHHCCCCEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEECC
GSMRGRPITVAATCADILARTLERCGVKVEILGFTTKAWKGGQSREKWLAGGKPQSPGRL
CCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCHHHHHCCCCCCCCCHH
NDLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALMWAHDRLLARSEQRRILMMI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCEEEEEE
SDGAPVDDSTLSVNPGNYLERHLRAVIEQIETRSPVELLAIGIGHDVTRYYRRAVTIVDA
ECCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHEECH
DELAGAMTEQLAALFEDESTRRRPGGARRAG
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1917840 [H]