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The map label for this gene is gloB [H]

Identifier: 150397750

GI number: 150397750

Start: 2650305

End: 2651075

Strand: Direct

Name: gloB [H]

Synonym: Smed_2552

Alternate gene names: 150397750

Gene position: 2650305-2651075 (Clockwise)

Preceding gene: 150397746

Following gene: 150397751

Centisome position: 70.08

GC content: 60.83

Gene sequence:

>771_bases
ATGGCCGCCCTCGAACTCGACCTCTTCCTCTGCCGCACCGACAATTACGGTGTGCTCCTCCACGATCGGCTGAGCGGCGC
GACGGCATCGATCGACGCACCGGAAGAAAGACCGATCCTCGACGCGCTCGAACGGCGCAGCTGGCAACTGACGCACATCC
TCACAACGCATCACCATGGCGACCATGTGGCCGGCAATATCGGTCTGAAGGAACGCTTCGGCGTGACCATCATCGGTCCG
AAGAACGAGTCTCCGAAGATCCCTGGTATTGACAGAAGCGTGGGCCATGGCGACCGCTTCGACTTCGCCGGCCACCCAGT
CGAGGTCATCGAAACGCCGGGACATACGGCCGGCCACGTCTGCTACCATTTCCCTGAGGACAAGCTGCTTTTCGCCGCCG
ATACGCTTTTTGCTCTGGGATGCGGCCGTCTGTTCGAAGGAACGCCCGAAACGATGTGGCAGTCGCTGAGCCGACTGATG
GCGCTACCGGATGACACGGCCATTTACTTCGGCCACGAATACACGCTCGCAAACGCGCGTTTCGCCGTCACCGTCGATCC
GGAAAACACGGCGCTGAAGGAACGAGCGGCCGAGATCGAGGAGACGCGATCGGACGGCGGATTCACTGCGCCCACAACCA
TGGGCCTTGAAAAGCGGACGAACCCGTTCCTGCGCGCCGATGATCCAAAGATCCGCGCCTTTCTGGGCATGGACAAGGCG
AGCGATGCCGCCGTCTTCGCAGAAATACGCAAACGCAAGGACAGTTTTTGA

Upstream 100 bases:

>100_bases
ATGTTAGACTCGAAGCGGTAGACTTCAAGGCGAGCCTCGCTACATTCGATCGAACGGGATCGCCCCGTCCCGATAACCGG
CACCGATCGGAGGACCAATC

Downstream 100 bases:

>100_bases
TGACGACCCGGGAGATGACGGCGGCAGCCATTATCGAAACCCTGAGGCTCGCGCGGCATCCTGAGGGTGGCTGGTATGGC
GAGACATTCCGCGACGCCGA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAALELDLFLCRTDNYGVLLHDRLSGATASIDAPEERPILDALERRSWQLTHILTTHHHGDHVAGNIGLKERFGVTIIGP
KNESPKIPGIDRSVGHGDRFDFAGHPVEVIETPGHTAGHVCYHFPEDKLLFAADTLFALGCGRLFEGTPETMWQSLSRLM
ALPDDTAIYFGHEYTLANARFAVTVDPENTALKERAAEIEETRSDGGFTAPTTMGLEKRTNPFLRADDPKIRAFLGMDKA
SDAAVFAEIRKRKDSF

Sequences:

>Translated_256_residues
MAALELDLFLCRTDNYGVLLHDRLSGATASIDAPEERPILDALERRSWQLTHILTTHHHGDHVAGNIGLKERFGVTIIGP
KNESPKIPGIDRSVGHGDRFDFAGHPVEVIETPGHTAGHVCYHFPEDKLLFAADTLFALGCGRLFEGTPETMWQSLSRLM
ALPDDTAIYFGHEYTLANARFAVTVDPENTALKERAAEIEETRSDGGFTAPTTMGLEKRTNPFLRADDPKIRAFLGMDKA
SDAAVFAEIRKRKDSF
>Mature_255_residues
AALELDLFLCRTDNYGVLLHDRLSGATASIDAPEERPILDALERRSWQLTHILTTHHHGDHVAGNIGLKERFGVTIIGPK
NESPKIPGIDRSVGHGDRFDFAGHPVEVIETPGHTAGHVCYHFPEDKLLFAADTLFALGCGRLFEGTPETMWQSLSRLMA
LPDDTAIYFGHEYTLANARFAVTVDPENTALKERAAEIEETRSDGGFTAPTTMGLEKRTNPFLRADDPKIRAFLGMDKAS
DAAVFAEIRKRKDSF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI14150041, Length=249, Percent_Identity=35.7429718875502, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=33.0769230769231, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=33.0769230769231, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI21703352, Length=255, Percent_Identity=33.7254901960784, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI116642887, Length=255, Percent_Identity=33.7254901960784, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI46361987, Length=188, Percent_Identity=36.7021276595745, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI41327741, Length=195, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786406, Length=246, Percent_Identity=38.2113821138211, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1787158, Length=146, Percent_Identity=30.8219178082192, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17536925, Length=261, Percent_Identity=37.5478927203065, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17538952, Length=206, Percent_Identity=27.6699029126214, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320478, Length=244, Percent_Identity=33.1967213114754, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=266, Percent_Identity=31.9548872180451, Blast_Score=102, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356335, Length=265, Percent_Identity=35.0943396226415, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24667711, Length=265, Percent_Identity=35.0943396226415, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24667703, Length=265, Percent_Identity=35.0943396226415, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI221330176, Length=172, Percent_Identity=31.9767441860465, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28312; Mature: 28181

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALELDLFLCRTDNYGVLLHDRLSGATASIDAPEERPILDALERRSWQLTHILTTHHHG
CCEEEEEEEEEECCCCEEEEEECCCCCEECCCCCCCCCHHHHHHHCCEEEEEEEEECCCC
DHVAGNIGLKERFGVTIIGPKNESPKIPGIDRSVGHGDRFDFAGHPVEVIETPGHTAGHV
CEEEECCCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCE
CYHFPEDKLLFAADTLFALGCGRLFEGTPETMWQSLSRLMALPDDTAIYFGHEYTLANAR
EEECCCCCEEEEHHHHHHHHCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEECEEEECCCE
FAVTVDPENTALKERAAEIEETRSDGGFTAPTTMGLEKRTNPFLRADDPKIRAFLGMDKA
EEEEECCCCHHHHHHHHHHHHHHCCCCEECCCCCCCHHCCCCCEECCCCCEEEEECCCCC
SDAAVFAEIRKRKDSF
CCHHHHHHHHHHCCCC
>Mature Secondary Structure 
AALELDLFLCRTDNYGVLLHDRLSGATASIDAPEERPILDALERRSWQLTHILTTHHHG
CEEEEEEEEEECCCCEEEEEECCCCCEECCCCCCCCCHHHHHHHCCEEEEEEEEECCCC
DHVAGNIGLKERFGVTIIGPKNESPKIPGIDRSVGHGDRFDFAGHPVEVIETPGHTAGHV
CEEEECCCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCE
CYHFPEDKLLFAADTLFALGCGRLFEGTPETMWQSLSRLMALPDDTAIYFGHEYTLANAR
EEECCCCCEEEEHHHHHHHHCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEECEEEECCCE
FAVTVDPENTALKERAAEIEETRSDGGFTAPTTMGLEKRTNPFLRADDPKIRAFLGMDKA
EEEEECCCCHHHHHHHHHHHHHHCCCCEECCCCCCCHHCCCCCEECCCCCEEEEECCCCC
SDAAVFAEIRKRKDSF
CCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]