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The map label for this gene is gluQ [H]

Identifier: 150397724

GI number: 150397724

Start: 2625257

End: 2626150

Strand: Direct

Name: gluQ [H]

Synonym: Smed_2526

Alternate gene names: 150397724

Gene position: 2625257-2626150 (Clockwise)

Preceding gene: 150397721

Following gene: 150397726

Centisome position: 69.42

GC content: 63.98

Gene sequence:

>894_bases
ATGCCGATTTCACGACCAGAGCAACCGGTTTTTCGCTTTGCGCCGAGCCCGAACGGTTTCCTGCATCTCGGCCACGCCCT
TTCCGCGATCATCAATCACGACATTGCGGCTGACTCGGGCGGCCGGTTTCTGCTGAGGATCGAGGACATCGACAGGACTC
GTTGCCGGCCGGAGTTCGAGGCCGCGATCCTCGACGATCTCGCCTGGCTCGAGCTCGAATGGGAGGAACCGGTGCGGCGC
CAGTCCGACCATCTCGATCTCTATGCCTCGGCGCTCGAGCATCTCAAAGCAATGCGTCTCGTCTATCCGTCCGCGATGAC
ACGCGGCGAGATCAGGGCCGCGGTCGCGGCGTCGGAGGCGAAAGGTTCCGCCTGGCCGCGCGATCCGGACGGAACGCCGC
TCTATCCCGGCCGCGAGCGCGGTTTCAGGCCCGATGATCAGGCGGCGATCCTGGCGAGTGGCCGTCCTTATGCCTGGCGG
CTGGACATGGAGGAGGCCGTGAAGCGTGCGGGCGGATCACTGAGCTGGCAAGAAAAGGGTTTTCGGGGGTCAGGTGAATA
CAGCCAGATCGCCGATCCGGCCGCCTGGGGCGATGTCGTCCTGTCGCGTTCGGATGCGCCGTCGAGCTATCATCTCTCGG
TCGTGGTCGACGATGCCGTGCAAGGTGTCACCCACATCGTGCGCGGCCGCGATCTTTATCACGCAACATCCGTGCATCGC
CTGCTGCAGCGCCTTCTTGAGCTTCCGGAGCCGGTCTACCACCATCACCGTCTTATCCTCGGCCCGGACGGACGGAAACT
GTCAAAGAGCAACCGCGACACCGGCATTGCCGCTTTTCGGGCTGCCGGGCACGGGCCTGCAGATATCAGGGCGATGGTTC
TCGGCGAGCAATGA

Upstream 100 bases:

>100_bases
CGGCGATTCCGGCCTCTATGTCCTCGTGCGTCCGGATGATTCGCATGCGTCCTCCAAAAAAAAAGTGATATTTATATCGC
TTCCATGGCAAAGCAAAACG

Downstream 100 bases:

>100_bases
GATAAGCCGATAGAGATCTACTCGCCGCGACCGGCCTGGAAACTGCGCCTGACCATGAGCTTCACCTTGTATTTCAGAAT
GGCGGCGTTGATTTCCGCGC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPISRPEQPVFRFAPSPNGFLHLGHALSAIINHDIAADSGGRFLLRIEDIDRTRCRPEFEAAILDDLAWLELEWEEPVRR
QSDHLDLYASALEHLKAMRLVYPSAMTRGEIRAAVAASEAKGSAWPRDPDGTPLYPGRERGFRPDDQAAILASGRPYAWR
LDMEEAVKRAGGSLSWQEKGFRGSGEYSQIADPAAWGDVVLSRSDAPSSYHLSVVVDDAVQGVTHIVRGRDLYHATSVHR
LLQRLLELPEPVYHHHRLILGPDGRKLSKSNRDTGIAAFRAAGHGPADIRAMVLGEQ

Sequences:

>Translated_297_residues
MPISRPEQPVFRFAPSPNGFLHLGHALSAIINHDIAADSGGRFLLRIEDIDRTRCRPEFEAAILDDLAWLELEWEEPVRR
QSDHLDLYASALEHLKAMRLVYPSAMTRGEIRAAVAASEAKGSAWPRDPDGTPLYPGRERGFRPDDQAAILASGRPYAWR
LDMEEAVKRAGGSLSWQEKGFRGSGEYSQIADPAAWGDVVLSRSDAPSSYHLSVVVDDAVQGVTHIVRGRDLYHATSVHR
LLQRLLELPEPVYHHHRLILGPDGRKLSKSNRDTGIAAFRAAGHGPADIRAMVLGEQ
>Mature_296_residues
PISRPEQPVFRFAPSPNGFLHLGHALSAIINHDIAADSGGRFLLRIEDIDRTRCRPEFEAAILDDLAWLELEWEEPVRRQ
SDHLDLYASALEHLKAMRLVYPSAMTRGEIRAAVAASEAKGSAWPRDPDGTPLYPGRERGFRPDDQAAILASGRPYAWRL
DMEEAVKRAGGSLSWQEKGFRGSGEYSQIADPAAWGDVVLSRSDAPSSYHLSVVVDDAVQGVTHIVRGRDLYHATSVHRL
LQRLLELPEPVYHHHRLILGPDGRKLSKSNRDTGIAAFRAAGHGPADIRAMVLGEQ

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=290, Percent_Identity=32.7586206896552, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1788743, Length=278, Percent_Identity=31.6546762589928, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI226510925, Length=266, Percent_Identity=32.7067669172932, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786895, Length=102, Percent_Identity=40.1960784313725, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=276, Percent_Identity=26.0869565217391, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324540, Length=286, Percent_Identity=27.972027972028, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24665321, Length=292, Percent_Identity=31.5068493150685, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 32925; Mature: 32794

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISRPEQPVFRFAPSPNGFLHLGHALSAIINHDIAADSGGRFLLRIEDIDRTRCRPEFE
CCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHCCCHHH
AAILDDLAWLELEWEEPVRRQSDHLDLYASALEHLKAMRLVYPSAMTRGEIRAAVAASEA
HHHHHCHHEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
KGSAWPRDPDGTPLYPGRERGFRPDDQAAILASGRPYAWRLDMEEAVKRAGGSLSWQEKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECHHHHHHHCCCCCCHHHCC
FRGSGEYSQIADPAAWGDVVLSRSDAPSSYHLSVVVDDAVQGVTHIVRGRDLYHATSVHR
CCCCCCHHHHCCCCCHHHHEEECCCCCCCEEEEEEECHHHHHHHHHHHCCCHHHHHHHHH
LLQRLLELPEPVYHHHRLILGPDGRKLSKSNRDTGIAAFRAAGHGPADIRAMVLGEQ
HHHHHHHCCCCHHHCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC
>Mature Secondary Structure 
PISRPEQPVFRFAPSPNGFLHLGHALSAIINHDIAADSGGRFLLRIEDIDRTRCRPEFE
CCCCCCCCEEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHCCCHHH
AAILDDLAWLELEWEEPVRRQSDHLDLYASALEHLKAMRLVYPSAMTRGEIRAAVAASEA
HHHHHCHHEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
KGSAWPRDPDGTPLYPGRERGFRPDDQAAILASGRPYAWRLDMEEAVKRAGGSLSWQEKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECHHHHHHHCCCCCCHHHCC
FRGSGEYSQIADPAAWGDVVLSRSDAPSSYHLSVVVDDAVQGVTHIVRGRDLYHATSVHR
CCCCCCHHHHCCCCCHHHHEEECCCCCCCEEEEEEECHHHHHHHHHHHCCCHHHHHHHHH
LLQRLLELPEPVYHHHRLILGPDGRKLSKSNRDTGIAAFRAAGHGPADIRAMVLGEQ
HHHHHHHCCCCHHHCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA