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The map label for this gene is nudG [H]

Identifier: 150397712

GI number: 150397712

Start: 2609970

End: 2610377

Strand: Reverse

Name: nudG [H]

Synonym: Smed_2514

Alternate gene names: 150397712

Gene position: 2610377-2609970 (Counterclockwise)

Preceding gene: 150397713

Following gene: 150397711

Centisome position: 69.02

GC content: 64.22

Gene sequence:

>408_bases
ATGCAGGATAGGAACATCGTGCTCGTCGCCGCTTGCGCGCTCGTCGATTCCGACGGACGTGTTCTGTTGGCGCAGCGCCC
GCAGGGCAAGCCTCTCGCCGGCCTCTGGGAGTTCCCGGGCGGTAAAGTCGAGAGTGGCGAGACACCCGAAGAGACGCTGA
TCCGCGAACTCGAAGAGGAGCTCGGGATCCGCACCAAGGTCGCGTGCCTCGCGCCGCTCACATTCGCGAGCCACGGCTAT
GACGAATTTCACCTGCTGATGCCGCTCTACGTCTGCCGCCGCTACGAGGGTTTCGCCGAAGGCCGCGAAGGCCAGGCCAT
CAAATGGGTGCGGCCCAAAGCGCTGCGCGACTATCCGATGCCGCCCGCCGACGAACCATTGATCCCGTTCCTGATGGACC
TGCTTTAG

Upstream 100 bases:

>100_bases
AGGTGGAGGCCGACAACGCGGCTGCGATCGAGCTTTATCGCAAGGCCGGTTTCACCGAGGTCTACCGCTATCTCTATCGT
ATGCCGAAGGTCTGAGGAAC

Downstream 100 bases:

>100_bases
ATCACGATGTCTTAGATCGGGGCGACCTAAGATCATGAACGTGATCGACTCCAAATAAATAGAGCGGATGCGGGCGGAAA
ACCGCACACACTTTTCCTCA

Product: NUDIX hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MQDRNIVLVAACALVDSDGRVLLAQRPQGKPLAGLWEFPGGKVESGETPEETLIRELEEELGIRTKVACLAPLTFASHGY
DEFHLLMPLYVCRRYEGFAEGREGQAIKWVRPKALRDYPMPPADEPLIPFLMDLL

Sequences:

>Translated_135_residues
MQDRNIVLVAACALVDSDGRVLLAQRPQGKPLAGLWEFPGGKVESGETPEETLIRELEEELGIRTKVACLAPLTFASHGY
DEFHLLMPLYVCRRYEGFAEGREGQAIKWVRPKALRDYPMPPADEPLIPFLMDLL
>Mature_135_residues
MQDRNIVLVAACALVDSDGRVLLAQRPQGKPLAGLWEFPGGKVESGETPEETLIRELEEELGIRTKVACLAPLTFASHGY
DEFHLLMPLYVCRRYEGFAEGREGQAIKWVRPKALRDYPMPPADEPLIPFLMDLL

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=125, Percent_Identity=40, Blast_Score=88, Evalue=2e-19,
Organism=Escherichia coli, GI1786288, Length=126, Percent_Identity=32.5396825396825, Blast_Score=76, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.-

Molecular weight: Translated: 15151; Mature: 15151

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRNIVLVAACALVDSDGRVLLAQRPQGKPLAGLWEFPGGKVESGETPEETLIRELEEE
CCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHH
LGIRTKVACLAPLTFASHGYDEFHLLMPLYVCRRYEGFAEGREGQAIKWVRPKALRDYPM
HCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHCCCC
PPADEPLIPFLMDLL
CCCCCCHHHHHHHHC
>Mature Secondary Structure
MQDRNIVLVAACALVDSDGRVLLAQRPQGKPLAGLWEFPGGKVESGETPEETLIRELEEE
CCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHH
LGIRTKVACLAPLTFASHGYDEFHLLMPLYVCRRYEGFAEGREGQAIKWVRPKALRDYPM
HCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHCCCC
PPADEPLIPFLMDLL
CCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]