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The map label for this gene is yiaO [H]

Identifier: 150397680

GI number: 150397680

Start: 2563919

End: 2564923

Strand: Reverse

Name: yiaO [H]

Synonym: Smed_2480

Alternate gene names: 150397680

Gene position: 2564923-2563919 (Counterclockwise)

Preceding gene: 150397681

Following gene: 150397679

Centisome position: 67.82

GC content: 58.21

Gene sequence:

>1005_bases
ATGAGAAAACTGCTACTAGCCACGACTGCCATCGCTCTCGGTCTTTCGTCCGCAGCGCCGGCCTTCGCCGAGTTCAACGA
ACGCAACATCCGGGTTTCGAACGGCATCAACGAAGATCATCCCGTCGGCAACGGCATCAAGGCGATGCAGGCCTGCCTCG
ATGAAAAGTCGGGCGGCAAGCTGAAGCTCACCGCCTTCTGGGGCGGGGCGCTCGGTGGCGACCTTCAGGCAACGCAGGCC
CTTCGTTCGGGCGTACAGGAAGCCGTCGTCACCTCGTCATCGCCGCTCGTCGGCATCATCCCGGCACTCGGCGTCTTCGA
CCTGCCGTTCCTCTTCGCCAGCGCACAGGAAGCCTATACGGTGCTCGATGGCGATTTCGGCGACATGATGAACGAGAAGC
TGGAGGCGGCCGGTCTGGTCAACCTCGCCTATTGGGAAAACGGCTTCCGCAACCTGTCGAACTCGGTTCGTCCGGTCACG
AAATGGGAAGACTTCGAAGGAATGAAGGTTCGCGTAATGCAGAACAACATCTTCCTCGACACCTTCCAGAACCTCGGCGC
CAACGCCACTCCGATGGCCTTCGGCGAAGTGTTCTCGGCGCTTGAAACCAAGGCGATCGACGCTCAGGAAAACCCCTATG
TGACGATCGATACCTCGAAGTTCTTCGAAGTGCAGAAATATGTGACCGAGACGAACCACGCCTACACGCCCTTCCTCTTC
CTGTTCTCGAAGCCGATCTTCGACAGCTATACGCCGGAAGAGCAGGCAGCCCTGCGTGAATGCGCAATCGTCGGCCGCGA
CGAAGAGCGCAAGGTGATCCAGGACCTCAACAAGAAGTCGCTGGAAAAGATCAAGGAAGCCGGGCTTGAAGTGAACGTCC
TGTCTGCTGAGGAACAGGCGCGCATTCGCGAAAAATCAAAGGTCGTCTATGAGAAACACAAGGCTGCGATCGGTGCCGAC
GTGGTCGACGCTATTCTCGCCAAGCTGGAAGAGATCCGCAAGTAA

Upstream 100 bases:

>100_bases
TGCTTTTCCTTCTGGTCCTTTTTCCGGACATCGTCATCGTGCCCGCACGCTGGCTCAACTGATCCTCAACCCCTCATCAA
TAAGTCCAAGGGAGGACATC

Downstream 100 bases:

>100_bases
CAGTAAAAGCCGGATCTGAATAAGCGGCTTTGTTCGACCCCTTCGAAACCCGCGGCCACACGGCCGCGGGTTTTCTTCAT
TGGAGCGACCGGCATAGACG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRKLLLATTAIALGLSSAAPAFAEFNERNIRVSNGINEDHPVGNGIKAMQACLDEKSGGKLKLTAFWGGALGGDLQATQA
LRSGVQEAVVTSSSPLVGIIPALGVFDLPFLFASAQEAYTVLDGDFGDMMNEKLEAAGLVNLAYWENGFRNLSNSVRPVT
KWEDFEGMKVRVMQNNIFLDTFQNLGANATPMAFGEVFSALETKAIDAQENPYVTIDTSKFFEVQKYVTETNHAYTPFLF
LFSKPIFDSYTPEEQAALRECAIVGRDEERKVIQDLNKKSLEKIKEAGLEVNVLSAEEQARIREKSKVVYEKHKAAIGAD
VVDAILAKLEEIRK

Sequences:

>Translated_334_residues
MRKLLLATTAIALGLSSAAPAFAEFNERNIRVSNGINEDHPVGNGIKAMQACLDEKSGGKLKLTAFWGGALGGDLQATQA
LRSGVQEAVVTSSSPLVGIIPALGVFDLPFLFASAQEAYTVLDGDFGDMMNEKLEAAGLVNLAYWENGFRNLSNSVRPVT
KWEDFEGMKVRVMQNNIFLDTFQNLGANATPMAFGEVFSALETKAIDAQENPYVTIDTSKFFEVQKYVTETNHAYTPFLF
LFSKPIFDSYTPEEQAALRECAIVGRDEERKVIQDLNKKSLEKIKEAGLEVNVLSAEEQARIREKSKVVYEKHKAAIGAD
VVDAILAKLEEIRK
>Mature_334_residues
MRKLLLATTAIALGLSSAAPAFAEFNERNIRVSNGINEDHPVGNGIKAMQACLDEKSGGKLKLTAFWGGALGGDLQATQA
LRSGVQEAVVTSSSPLVGIIPALGVFDLPFLFASAQEAYTVLDGDFGDMMNEKLEAAGLVNLAYWENGFRNLSNSVRPVT
KWEDFEGMKVRVMQNNIFLDTFQNLGANATPMAFGEVFSALETKAIDAQENPYVTIDTSKFFEVQKYVTETNHAYTPFLF
LFSKPIFDSYTPEEQAALRECAIVGRDEERKVIQDLNKKSLEKIKEAGLEVNVLSAEEQARIREKSKVVYEKHKAAIGAD
VVDAILAKLEEIRK

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=245, Percent_Identity=38.7755102040816, Blast_Score=188, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36697; Mature: 36697

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLLATTAIALGLSSAAPAFAEFNERNIRVSNGINEDHPVGNGIKAMQACLDEKSGGK
CCHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCE
LKLTAFWGGALGGDLQATQALRSGVQEAVVTSSSPLVGIIPALGVFDLPFLFASAQEAYT
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHE
VLDGDFGDMMNEKLEAAGLVNLAYWENGFRNLSNSVRPVTKWEDFEGMKVRVMQNNIFLD
EECCCHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEE
TFQNLGANATPMAFGEVFSALETKAIDAQENPYVTIDTSKFFEVQKYVTETNHAYTPFLF
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHH
LFSKPIFDSYTPEEQAALRECAIVGRDEERKVIQDLNKKSLEKIKEAGLEVNVLSAEEQA
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCCEEEEECCHHHH
RIREKSKVVYEKHKAAIGADVVDAILAKLEEIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKLLLATTAIALGLSSAAPAFAEFNERNIRVSNGINEDHPVGNGIKAMQACLDEKSGGK
CCHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCE
LKLTAFWGGALGGDLQATQALRSGVQEAVVTSSSPLVGIIPALGVFDLPFLFASAQEAYT
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHE
VLDGDFGDMMNEKLEAAGLVNLAYWENGFRNLSNSVRPVTKWEDFEGMKVRVMQNNIFLD
EECCCHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEE
TFQNLGANATPMAFGEVFSALETKAIDAQENPYVTIDTSKFFEVQKYVTETNHAYTPFLF
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHH
LFSKPIFDSYTPEEQAALRECAIVGRDEERKVIQDLNKKSLEKIKEAGLEVNVLSAEEQA
HHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCCEEEEECCHHHH
RIREKSKVVYEKHKAAIGADVVDAILAKLEEIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]