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The map label for this gene is gldA [H]

Identifier: 150397660

GI number: 150397660

Start: 2542389

End: 2543483

Strand: Direct

Name: gldA [H]

Synonym: Smed_2460

Alternate gene names: 150397660

Gene position: 2542389-2543483 (Clockwise)

Preceding gene: 150397659

Following gene: 150397661

Centisome position: 67.23

GC content: 65.21

Gene sequence:

>1095_bases
ATGGCACGGGCATTCGGCGGTCCAAACAAATACATTCAGCGCGCAGGCGAGATCGACAAGCTGGCGGACTATCTGGCACC
GCTCGGCAAGCGCGCGCTCGTGCTGATCGACCGCGTGCTGTTCGAAAAGCTCAGTGAACGTATTGCCAAAAACTGCGACG
ACAAGCTCGACCTCCACTTCGAGCGGTTCGGCGGCGAATGCTGCACCTCGGAGATCGAGCGTGTGCGCGCGGCCGCAGCC
GAGCACCGCAGCGATATCCTCGTGGGTGTGGGCGGCGGCAAGACCGCCGACACCGCCAAGATCGTCGCCATCGACACGGA
TGCGCGCATCGTCATCGCACCGACCATCGCCTCCACCGATGCGCCCTGCAGCGCGATTGCGGTGCGCTATTCCGAGCATG
GCGTCTATGAGGAATCGCTCCGCCTGCCCCGCAACCCGGACGTGGTCGTCGTCGACTCCGCGCTGGTGGCAGCCGCGCCT
GCACGCTTCCTGGTCGCCGGTATCGGCGATGCGCTTTCGACCTGGTTCGAGGCCCGCTCCAATATAGAGTCACGCACAGA
CAACTATGTCGCTGGCGGGTTTCCGGCAACCGAGGCCGGCATGGCTATCGCGCGGCATTGCCAGGAGGTGCTGATGCGCG
ACGCCGTAAAGGCCAAGGTCGCCGTCGAGGCCGGTCTGCTCACCCGCGCTGTGGAGAATATCATCGAGGCGAACACGCTG
CTGTCGGGCCTCGGCTTCGAGAATTGCGGCTGCTCGGCGGCGCACGGCATCCATGACGGCTTGACCGTGCTCGAAGAGGT
CCATGGTTTATTCCACGGCGAGAAGGTCGCTTTCGGCACGCTCTGCCTGCTGATGCTGGAAAACCGCGACCGGGGCGAAA
TCGAGGCGATGATACGCTTCTGCCGCAGCGTGGGTCTGCCGACCAGACTGGCGGACCTAGGCATCGTGGATGACGTGCCC
GCCAAGATCGGGCGCGTGGCGGATGCCGCCTGCCGGCCCGGCAACATCATCTATGCCACGCCGGTTGCCGTCACCGCTCC
CGCGGTGCGCGACGCGATCCTGGCGCTCGATGCGCTGTCGCGCAGCATCGATTGA

Upstream 100 bases:

>100_bases
GACGCCGCCAGTTACATCACCGGATCGGTGCTCACCATCGACGGCGGCTACACCATCCATTGACCTTCGCACACTCAAGC
AAAGATATGGGAGTATTCGA

Downstream 100 bases:

>100_bases
GCAGCGGGAGGAAAAGCATGGCCGATAGAAAGCCGCTCGTCATGATCACCGGCGCAAGCGCCGGTATCGGGCAGGAGACT
GCGCGCGTCTTTTCGGCCGC

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MARAFGGPNKYIQRAGEIDKLADYLAPLGKRALVLIDRVLFEKLSERIAKNCDDKLDLHFERFGGECCTSEIERVRAAAA
EHRSDILVGVGGGKTADTAKIVAIDTDARIVIAPTIASTDAPCSAIAVRYSEHGVYEESLRLPRNPDVVVVDSALVAAAP
ARFLVAGIGDALSTWFEARSNIESRTDNYVAGGFPATEAGMAIARHCQEVLMRDAVKAKVAVEAGLLTRAVENIIEANTL
LSGLGFENCGCSAAHGIHDGLTVLEEVHGLFHGEKVAFGTLCLLMLENRDRGEIEAMIRFCRSVGLPTRLADLGIVDDVP
AKIGRVADAACRPGNIIYATPVAVTAPAVRDAILALDALSRSID

Sequences:

>Translated_364_residues
MARAFGGPNKYIQRAGEIDKLADYLAPLGKRALVLIDRVLFEKLSERIAKNCDDKLDLHFERFGGECCTSEIERVRAAAA
EHRSDILVGVGGGKTADTAKIVAIDTDARIVIAPTIASTDAPCSAIAVRYSEHGVYEESLRLPRNPDVVVVDSALVAAAP
ARFLVAGIGDALSTWFEARSNIESRTDNYVAGGFPATEAGMAIARHCQEVLMRDAVKAKVAVEAGLLTRAVENIIEANTL
LSGLGFENCGCSAAHGIHDGLTVLEEVHGLFHGEKVAFGTLCLLMLENRDRGEIEAMIRFCRSVGLPTRLADLGIVDDVP
AKIGRVADAACRPGNIIYATPVAVTAPAVRDAILALDALSRSID
>Mature_363_residues
ARAFGGPNKYIQRAGEIDKLADYLAPLGKRALVLIDRVLFEKLSERIAKNCDDKLDLHFERFGGECCTSEIERVRAAAAE
HRSDILVGVGGGKTADTAKIVAIDTDARIVIAPTIASTDAPCSAIAVRYSEHGVYEESLRLPRNPDVVVVDSALVAAAPA
RFLVAGIGDALSTWFEARSNIESRTDNYVAGGFPATEAGMAIARHCQEVLMRDAVKAKVAVEAGLLTRAVENIIEANTLL
SGLGFENCGCSAAHGIHDGLTVLEEVHGLFHGEKVAFGTLCLLMLENRDRGEIEAMIRFCRSVGLPTRLADLGIVDDVPA
KIGRVADAACRPGNIIYATPVAVTAPAVRDAILALDALSRSID

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Exhibits a rather broad substrate specificity since it can also oxidize 1,2-prop

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=362, Percent_Identity=45.8563535911602, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1786815, Length=378, Percent_Identity=28.8359788359788, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 38758; Mature: 38627

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAFGGPNKYIQRAGEIDKLADYLAPLGKRALVLIDRVLFEKLSERIAKNCDDKLDLHF
CCCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ERFGGECCTSEIERVRAAAAEHRSDILVGVGGGKTADTAKIVAIDTDARIVIAPTIASTD
HHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCC
APCSAIAVRYSEHGVYEESLRLPRNPDVVVVDSALVAAAPARFLVAGIGDALSTWFEARS
CCHHHHEEEHHHCCCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIESRTDNYVAGGFPATEAGMAIARHCQEVLMRDAVKAKVAVEAGLLTRAVENIIEANTL
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGFENCGCSAAHGIHDGLTVLEEVHGLFHGEKVAFGTLCLLMLENRDRGEIEAMIRF
HHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
CRSVGLPTRLADLGIVDDVPAKIGRVADAACRPGNIIYATPVAVTAPAVRDAILALDALS
HHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
RSID
HCCC
>Mature Secondary Structure 
ARAFGGPNKYIQRAGEIDKLADYLAPLGKRALVLIDRVLFEKLSERIAKNCDDKLDLHF
CCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ERFGGECCTSEIERVRAAAAEHRSDILVGVGGGKTADTAKIVAIDTDARIVIAPTIASTD
HHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCC
APCSAIAVRYSEHGVYEESLRLPRNPDVVVVDSALVAAAPARFLVAGIGDALSTWFEARS
CCHHHHEEEHHHCCCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIESRTDNYVAGGFPATEAGMAIARHCQEVLMRDAVKAKVAVEAGLLTRAVENIIEANTL
HHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGFENCGCSAAHGIHDGLTVLEEVHGLFHGEKVAFGTLCLLMLENRDRGEIEAMIRF
HHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
CRSVGLPTRLADLGIVDDVPAKIGRVADAACRPGNIIYATPVAVTAPAVRDAILALDALS
HHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
RSID
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA