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The map label for this gene is potD-B [H]

Identifier: 150397609

GI number: 150397609

Start: 2484504

End: 2485583

Strand: Reverse

Name: potD-B [H]

Synonym: Smed_2409

Alternate gene names: 150397609

Gene position: 2485583-2484504 (Counterclockwise)

Preceding gene: 150397610

Following gene: 150397608

Centisome position: 65.72

GC content: 61.67

Gene sequence:

>1080_bases
ATGATCATCAATTCCATCAAGCGGCGCACGCTGCTCGGTGCGGGGCTTGCCGGCGCCTCGGTGCTGGCGATGCCCTCGGT
GCTGCGGGCGCAAGACAGGTCGCTGAAAGTCGGCGTCTATGGCGGCTACTTCAAGGACTCGTTCGACAAAAACATCTTCC
CCGAATTCACCAAGGCATCGGGCATCGCGATCGAATCCGTCGCCGAGCCTACCGGCGAGGCGTGGCTCGTCCAGCTCGAA
CAGGCCGCCCGTGCCGGACAGGCGCCCGCGGACGTTTCCATGATGTCGCAGGTGGCGATGCTGAAAGGGCAGGCGACTGA
TCTCTGGACCCCGATCGACATGGCGAAGATCAAGAACGGCTCGAACCTGATCGAGCGATTCGTCAACAAGTATCCGGACG
GCCGCATCGCCGGTATCGGAGCCGTCTCCTGGTACATCACGCTCGTGACCAACACCGACAGCTACAAGGAGGCGCCGACC
TCATGGGAGGCGTTCTGGGATCCGGCGAATGCCGACAAGCTCGGTCTCCTGGCGCTGGTTTCCAATTCCTTCCTGCTCGA
AGTAACCGCCAAGACCTTCATGGGCGGCACCAATGCGTTGGACACGGAAGAAGGGATTCTCAAGGCATTCGAGAAGCTGG
CCGAGGTGAAGCCGAACGTCCGCCTCTGGTACCGCGACGAAGCGCAGTTCGAGCAGGCGCTGAAATCGGGCGAAATCCCG
ATGGGACAATATTATCATGACGTCACGGGCCTCGCGGCGGCCGACGGTCACCCGGTCCGTTCCACCTTCCCGAAGGAGGG
CGGCATCCAGGATTCCGGCTGCTGGGCCGTGTCGCGTGCCTCTCAGAAGGTCGAAGAAGCGCATATCTTCATCGATTACA
TGTGCCAGCCCGCGGTTCAGGCAACGCTCTCGCGCAAGGTTGGCACCTCGCCGACGGTCAAGCGCGAGTCCACCGATCTG
ACGGACAAGGAGTTTGCCGGCGTTTCCTCGGACATCGAGCCGATCGTTCCGCGCTACGACCTCTACCAGACGAAGTCGGA
TTGGCTGAACCAGAAATGGACGGAGCTGATCGTCGGCTGA

Upstream 100 bases:

>100_bases
TTGACATTCCGCTGATGCGCGGCGGGGACCAAAAGCGCAAAGAATCAACTAGAACAAGGGAACGCGGCCAACAGCCGCCA
AAGAACAAAGGAGACATGCG

Downstream 100 bases:

>100_bases
GACCTGACACGGCGACGCCGCCTCGGTCTTACCGGGGCGGCGCATTGGACGCACGGACCTTAAACCGGGAGCGGTTTAGG
GATAAGCTGATGTGGAATCT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIINSIKRRTLLGAGLAGASVLAMPSVLRAQDRSLKVGVYGGYFKDSFDKNIFPEFTKASGIAIESVAEPTGEAWLVQLE
QAARAGQAPADVSMMSQVAMLKGQATDLWTPIDMAKIKNGSNLIERFVNKYPDGRIAGIGAVSWYITLVTNTDSYKEAPT
SWEAFWDPANADKLGLLALVSNSFLLEVTAKTFMGGTNALDTEEGILKAFEKLAEVKPNVRLWYRDEAQFEQALKSGEIP
MGQYYHDVTGLAAADGHPVRSTFPKEGGIQDSGCWAVSRASQKVEEAHIFIDYMCQPAVQATLSRKVGTSPTVKRESTDL
TDKEFAGVSSDIEPIVPRYDLYQTKSDWLNQKWTELIVG

Sequences:

>Translated_359_residues
MIINSIKRRTLLGAGLAGASVLAMPSVLRAQDRSLKVGVYGGYFKDSFDKNIFPEFTKASGIAIESVAEPTGEAWLVQLE
QAARAGQAPADVSMMSQVAMLKGQATDLWTPIDMAKIKNGSNLIERFVNKYPDGRIAGIGAVSWYITLVTNTDSYKEAPT
SWEAFWDPANADKLGLLALVSNSFLLEVTAKTFMGGTNALDTEEGILKAFEKLAEVKPNVRLWYRDEAQFEQALKSGEIP
MGQYYHDVTGLAAADGHPVRSTFPKEGGIQDSGCWAVSRASQKVEEAHIFIDYMCQPAVQATLSRKVGTSPTVKRESTDL
TDKEFAGVSSDIEPIVPRYDLYQTKSDWLNQKWTELIVG
>Mature_359_residues
MIINSIKRRTLLGAGLAGASVLAMPSVLRAQDRSLKVGVYGGYFKDSFDKNIFPEFTKASGIAIESVAEPTGEAWLVQLE
QAARAGQAPADVSMMSQVAMLKGQATDLWTPIDMAKIKNGSNLIERFVNKYPDGRIAGIGAVSWYITLVTNTDSYKEAPT
SWEAFWDPANADKLGLLALVSNSFLLEVTAKTFMGGTNALDTEEGILKAFEKLAEVKPNVRLWYRDEAQFEQALKSGEIP
MGQYYHDVTGLAAADGHPVRSTFPKEGGIQDSGCWAVSRASQKVEEAHIFIDYMCQPAVQATLSRKVGTSPTVKRESTDL
TDKEFAGVSSDIEPIVPRYDLYQTKSDWLNQKWTELIVG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39334; Mature: 39334

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINSIKRRTLLGAGLAGASVLAMPSVLRAQDRSLKVGVYGGYFKDSFDKNIFPEFTKAS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCHHCCC
GIAIESVAEPTGEAWLVQLEQAARAGQAPADVSMMSQVAMLKGQATDLWTPIDMAKIKNG
CCCHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
SNLIERFVNKYPDGRIAGIGAVSWYITLVTNTDSYKEAPTSWEAFWDPANADKLGLLALV
HHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCHHHHCCCCCCCHHEEHEEC
SNSFLLEVTAKTFMGGTNALDTEEGILKAFEKLAEVKPNVRLWYRDEAQFEQALKSGEIP
CCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCC
MGQYYHDVTGLAAADGHPVRSTFPKEGGIQDSGCWAVSRASQKVEEAHIFIDYMCQPAVQ
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHH
ATLSRKVGTSPTVKRESTDLTDKEFAGVSSDIEPIVPRYDLYQTKSDWLNQKWTELIVG
HHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIINSIKRRTLLGAGLAGASVLAMPSVLRAQDRSLKVGVYGGYFKDSFDKNIFPEFTKAS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCHHCCC
GIAIESVAEPTGEAWLVQLEQAARAGQAPADVSMMSQVAMLKGQATDLWTPIDMAKIKNG
CCCHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
SNLIERFVNKYPDGRIAGIGAVSWYITLVTNTDSYKEAPTSWEAFWDPANADKLGLLALV
HHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCHHHHCCCCCCCHHEEHEEC
SNSFLLEVTAKTFMGGTNALDTEEGILKAFEKLAEVKPNVRLWYRDEAQFEQALKSGEIP
CCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCCC
MGQYYHDVTGLAAADGHPVRSTFPKEGGIQDSGCWAVSRASQKVEEAHIFIDYMCQPAVQ
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHH
ATLSRKVGTSPTVKRESTDLTDKEFAGVSSDIEPIVPRYDLYQTKSDWLNQKWTELIVG
HHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]