Click here to switch to the map view.

The map label for this gene is ureF

Identifier: 150397578

GI number: 150397578

Start: 2458164

End: 2458835

Strand: Reverse

Name: ureF

Synonym: Smed_2378

Alternate gene names: 150397578

Gene position: 2458835-2458164 (Counterclockwise)

Preceding gene: 150397579

Following gene: 150397577

Centisome position: 65.02

GC content: 63.54

Gene sequence:

>672_bases
ATGGCTGAACATGCCGATACGCAGGCGTTGCTGCGCCTCGTTACCTGGCTCTCGCCCGCCTTTCCCGTCGGCTCCTTCTC
CTATTCCGGCGGATTGGAGCAGGCAATTCACCAAGGATTGGTCACGAGCGCCGATGATTTGCGGCTTTGGTGCGAAACGC
TCCTGGAGCGCGGCAACACCTGGAACGATGCGCTGCTTCTATCGGAAAGTTACCGAGCATACGGTGATGCCCAGCGGCTG
ATTGCGGTTTCGGAGCTTGCCGAGGCGCTTGCCGGATCGCGCGAACGGCATATGGAAACCATGCTCCTCGGCGAGGCTTT
CCTGGCTGCCGCCGGACATTGGCCGCATCCGTCGCTCGCAGTCCTCGGAACAAAGGCTGCGTATCCGGTGTCCGTGGGGG
CGGTTGCCGGAGCGCATCGTACCGGCCTCAAGCCGGCGCTCGCCGCCTTTCTCAATGCGACAGTATCGAACGCCGTTTCG
GTCGCCATCCGCTGCGGCATTACCGGCCAGCGCGACGGCGTCGGCGTTCTTGCGCGCGTCGAGGACACGATCGGCGCGGT
TGTTGCGCGCGCCGCGGCCGCCTCGCTTGAGGACCTGGGCGGCGCGACGTTCATTGCGGAAATTGCGTCACTGAAGCATG
AAAACCTGCATTCGCGCCTGTTCCGCTCATAA

Upstream 100 bases:

>100_bases
ATGCTCGAAGGCCTTGGCGCTACCGTCACGGAAGTGAACTTACCCTTCCACCCTCTACGCGGCGCCTATCACCAGCACGG
CCACGGCGACCGTCACAGCC

Downstream 100 bases:

>100_bases
TGCAGGAACAGATAGTCGAAAGGATAGAATTGATGCCATCTAAAAACGGTCCGCTTCGCGTTGGTATCGGTGGTCCGGTC
GGCTCCGGTAAGACGGCGCT

Product: urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MAEHADTQALLRLVTWLSPAFPVGSFSYSGGLEQAIHQGLVTSADDLRLWCETLLERGNTWNDALLLSESYRAYGDAQRL
IAVSELAEALAGSRERHMETMLLGEAFLAAAGHWPHPSLAVLGTKAAYPVSVGAVAGAHRTGLKPALAAFLNATVSNAVS
VAIRCGITGQRDGVGVLARVEDTIGAVVARAAAASLEDLGGATFIAEIASLKHENLHSRLFRS

Sequences:

>Translated_223_residues
MAEHADTQALLRLVTWLSPAFPVGSFSYSGGLEQAIHQGLVTSADDLRLWCETLLERGNTWNDALLLSESYRAYGDAQRL
IAVSELAEALAGSRERHMETMLLGEAFLAAAGHWPHPSLAVLGTKAAYPVSVGAVAGAHRTGLKPALAAFLNATVSNAVS
VAIRCGITGQRDGVGVLARVEDTIGAVVARAAAASLEDLGGATFIAEIASLKHENLHSRLFRS
>Mature_222_residues
AEHADTQALLRLVTWLSPAFPVGSFSYSGGLEQAIHQGLVTSADDLRLWCETLLERGNTWNDALLLSESYRAYGDAQRLI
AVSELAEALAGSRERHMETMLLGEAFLAAAGHWPHPSLAVLGTKAAYPVSVGAVAGAHRTGLKPALAAFLNATVSNAVSV
AIRCGITGQRDGVGVLARVEDTIGAVVARAAAASLEDLGGATFIAEIASLKHENLHSRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_SINMW (A6UC30)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328045.1
- STRING:   A6UC30
- GeneID:   5323239
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_2378
- eggNOG:   COG0830
- HOGENOM:   HBG709295
- OMA:   WLSPAFP
- ProtClustDB:   CLSK2484746
- BioCyc:   SMED366394:SMED_2378-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 23469; Mature: 23338

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHADTQALLRLVTWLSPAFPVGSFSYSGGLEQAIHQGLVTSADDLRLWCETLLERGNT
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
WNDALLLSESYRAYGDAQRLIAVSELAEALAGSRERHMETMLLGEAFLAAAGHWPHPSLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCE
VLGTKAAYPVSVGAVAGAHRTGLKPALAAFLNATVSNAVSVAIRCGITGQRDGVGVLARV
EEECCCCCCEEHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEHHHH
EDTIGAVVARAAAASLEDLGGATFIAEIASLKHENLHSRLFRS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEHADTQALLRLVTWLSPAFPVGSFSYSGGLEQAIHQGLVTSADDLRLWCETLLERGNT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
WNDALLLSESYRAYGDAQRLIAVSELAEALAGSRERHMETMLLGEAFLAAAGHWPHPSLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCE
VLGTKAAYPVSVGAVAGAHRTGLKPALAAFLNATVSNAVSVAIRCGITGQRDGVGVLARV
EEECCCCCCEEHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEHHHH
EDTIGAVVARAAAASLEDLGGATFIAEIASLKHENLHSRLFRS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA