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The map label for this gene is rpoE [H]

Identifier: 150397545

GI number: 150397545

Start: 2422053

End: 2422607

Strand: Reverse

Name: rpoE [H]

Synonym: Smed_2345

Alternate gene names: 150397545

Gene position: 2422607-2422053 (Counterclockwise)

Preceding gene: 150397546

Following gene: 150397544

Centisome position: 64.06

GC content: 59.82

Gene sequence:

>555_bases
ATGTCATCCGAAAATCAAGAGTTCAAGCGTGAGATGCTTGCCGCGCTGCCGAGCCTGCGCGCCTTCGCAATGTCGCTGAT
CGGACGGCACGACCGCGCCGATGACCTCGTGCAGGACACGATTATGAAGGCCTGGGCGAAGCAGGACCATTTTCAGATCG
GCACCAATATGAAGGCGTGGCTATTCACGATCCTGCGCAACGAGCTTTACAGCCAGATGCGCAAGCGTGGGCGTGAGGTG
CAGGACAGCGACGGTCATTTGACGGAAACGCTTGCCCATCACCCGGAGCAGTACGGCTCGCTCGATCTGCAGGATTTCCG
CCGCGCGCTCGACCAGTTGCCGCCTGACCAGCGCGAAGCGATCATTCTTGTAGGGGCCTCGGGCTTTTCCTATGAGGAGG
CGGCCACGATCTGCGGCTGTGCTCTTGGAACGATCAAGAGCCGCGTCAACCGGGCGCGCCAGCGGCTGCAGGAAATTCTC
CAGGTCAGAGGAGAAAACGATTATGGGCCGGACGAGACTTCAGCACCCATTACTTCGCGCGCCTTCGTTTCCTGA

Upstream 100 bases:

>100_bases
GTGCAGGAGGAAGCGATACCGGCACGCTTTCTCGATCTACTGGAAAAGCTGGAAGCCGCGGAACAGCGATCGACTCTGCA
GGGCAAGGAGTAAGCGCCGG

Downstream 100 bases:

>100_bases
TTGGGCCCGCCTGGCCCATAAAGCGGCTGTCATGCGGTAAAGGGGTGCCGGATCATACCTGGCGCCGCAGGCGGTAGTTC
GAATAATAAATCGGAGATTT

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSSENQEFKREMLAALPSLRAFAMSLIGRHDRADDLVQDTIMKAWAKQDHFQIGTNMKAWLFTILRNELYSQMRKRGREV
QDSDGHLTETLAHHPEQYGSLDLQDFRRALDQLPPDQREAIILVGASGFSYEEAATICGCALGTIKSRVNRARQRLQEIL
QVRGENDYGPDETSAPITSRAFVS

Sequences:

>Translated_184_residues
MSSENQEFKREMLAALPSLRAFAMSLIGRHDRADDLVQDTIMKAWAKQDHFQIGTNMKAWLFTILRNELYSQMRKRGREV
QDSDGHLTETLAHHPEQYGSLDLQDFRRALDQLPPDQREAIILVGASGFSYEEAATICGCALGTIKSRVNRARQRLQEIL
QVRGENDYGPDETSAPITSRAFVS
>Mature_183_residues
SSENQEFKREMLAALPSLRAFAMSLIGRHDRADDLVQDTIMKAWAKQDHFQIGTNMKAWLFTILRNELYSQMRKRGREVQ
DSDGHLTETLAHHPEQYGSLDLQDFRRALDQLPPDQREAIILVGASGFSYEEAATICGCALGTIKSRVNRARQRLQEILQ
VRGENDYGPDETSAPITSRAFVS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=154, Percent_Identity=31.1688311688312, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 20883; Mature: 20752

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSENQEFKREMLAALPSLRAFAMSLIGRHDRADDLVQDTIMKAWAKQDHFQIGTNMKAW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHCCCCHHHH
LFTILRNELYSQMRKRGREVQDSDGHLTETLAHHPEQYGSLDLQDFRRALDQLPPDQREA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHCCCCHHHHHHHHHHCCCCCCCE
IILVGASGFSYEEAATICGCALGTIKSRVNRARQRLQEILQVRGENDYGPDETSAPITSR
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
AFVS
HHCC
>Mature Secondary Structure 
SSENQEFKREMLAALPSLRAFAMSLIGRHDRADDLVQDTIMKAWAKQDHFQIGTNMKAW
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHCCCCHHHH
LFTILRNELYSQMRKRGREVQDSDGHLTETLAHHPEQYGSLDLQDFRRALDQLPPDQREA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHCCCCHHHHHHHHHHCCCCCCCE
IILVGASGFSYEEAATICGCALGTIKSRVNRARQRLQEILQVRGENDYGPDETSAPITSR
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
AFVS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]