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The map label for this gene is opuAB [H]

Identifier: 150397506

GI number: 150397506

Start: 2386055

End: 2386900

Strand: Direct

Name: opuAB [H]

Synonym: Smed_2306

Alternate gene names: 150397506

Gene position: 2386055-2386900 (Clockwise)

Preceding gene: 150397505

Following gene: 150397507

Centisome position: 63.09

GC content: 61.47

Gene sequence:

>846_bases
GTGGAACTCCTGACTGAAAACCCAATCCCGATCGGCGCCTGGGCGAAGGCCTTTGTCGACTGGCTGACGACGAATTTCGA
CCTTTTCTTCGACCAGCTCGCCAATGTGCTTTCGGGCATCATTTCCGTCCTGCTCTACATTCTCCAAACGCCGCATCCGC
TCATCGTCATCGCCATCGTGACGGCGCTGGCATGGTGGTTTCGCCGGTCCATCGGCATTGCCGTTTTCACCTGTCTCGGA
ATGCTGCTGATCGTCAATCAGGGATACTGGCAGGAGACCACGGAAACGCTGGCCCTGGTGCTTGCGGCGACCTTCGTCAG
CATGGCCGTCGGGGTGCCGCTCGGTATAGCCGCCGCGCGCCGCGCCTGGATTTACTCGTTCATGCGCCCGATCCTGGACC
TGATGCAGACGATCCCCACCTTCGTCTACCTCATCCCGGCGCTGATTCTCTTCGGTCTCGGCATGGTACCCGGGCTGATC
GCCACCGTTATCTTCGCCATACCCGCGCCAATCCGGCTGACGCGGCTCGGTATCATCTCGACACCGCCTGCGCTTGTGGA
GGCCGCAGAATCCTTCGGCGCCACGCCCTGGCAGGTGCTGCGCAAGGTAGAACTTCCCTTCGCCATGCCGCAGATCATGG
CGGGTCTCACGCAGACGATCATGCTGTCCCTTTCGATGGTGGTGATCGCGGCGCTCGTCGGAGCCGACGGCCTCGGAGTG
CCGGTACTCAGGGCTCTGAACACGGTGAACATCGCCAGGGGATTCGAGTCCGGCCTCTGCATCGTCATTCTTGCGATCGT
CCTCGACCGCCTTTTCCGTTCGTCCGATGAAGGAGAAGGCGCATGA

Upstream 100 bases:

>100_bases
GCGTTATGGGGATGCGTGCAGTGAGGCGGGGAAACACCCCGCCTTTTTTCCACCGGTCTTCCGACCTGAGTGCTGACCCT
GGCAAGAGGGACCCTGTTTC

Downstream 100 bases:

>100_bases
CCGACGCCGTCATTTTCAAGAATGTCGACATCATCTTCGGCAAAAATCCGCAGATCGCAACGCAAATGGTCGATCAGGGC
AAGACGCGGGACGAGATCGG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MELLTENPIPIGAWAKAFVDWLTTNFDLFFDQLANVLSGIISVLLYILQTPHPLIVIAIVTALAWWFRRSIGIAVFTCLG
MLLIVNQGYWQETTETLALVLAATFVSMAVGVPLGIAAARRAWIYSFMRPILDLMQTIPTFVYLIPALILFGLGMVPGLI
ATVIFAIPAPIRLTRLGIISTPPALVEAAESFGATPWQVLRKVELPFAMPQIMAGLTQTIMLSLSMVVIAALVGADGLGV
PVLRALNTVNIARGFESGLCIVILAIVLDRLFRSSDEGEGA

Sequences:

>Translated_281_residues
MELLTENPIPIGAWAKAFVDWLTTNFDLFFDQLANVLSGIISVLLYILQTPHPLIVIAIVTALAWWFRRSIGIAVFTCLG
MLLIVNQGYWQETTETLALVLAATFVSMAVGVPLGIAAARRAWIYSFMRPILDLMQTIPTFVYLIPALILFGLGMVPGLI
ATVIFAIPAPIRLTRLGIISTPPALVEAAESFGATPWQVLRKVELPFAMPQIMAGLTQTIMLSLSMVVIAALVGADGLGV
PVLRALNTVNIARGFESGLCIVILAIVLDRLFRSSDEGEGA
>Mature_281_residues
MELLTENPIPIGAWAKAFVDWLTTNFDLFFDQLANVLSGIISVLLYILQTPHPLIVIAIVTALAWWFRRSIGIAVFTCLG
MLLIVNQGYWQETTETLALVLAATFVSMAVGVPLGIAAARRAWIYSFMRPILDLMQTIPTFVYLIPALILFGLGMVPGLI
ATVIFAIPAPIRLTRLGIISTPPALVEAAESFGATPWQVLRKVELPFAMPQIMAGLTQTIMLSLSMVVIAALVGADGLGV
PVLRALNTVNIARGFESGLCIVILAIVLDRLFRSSDEGEGA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=270, Percent_Identity=44.8148148148148, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1788449, Length=194, Percent_Identity=31.4432989690722, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788451, Length=198, Percent_Identity=30.8080808080808, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=33.5526315789474, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30391; Mature: 30391

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLTENPIPIGAWAKAFVDWLTTNFDLFFDQLANVLSGIISVLLYILQTPHPLIVIAIV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TALAWWFRRSIGIAVFTCLGMLLIVNQGYWQETTETLALVLAATFVSMAVGVPLGIAAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RAWIYSFMRPILDLMQTIPTFVYLIPALILFGLGMVPGLIATVIFAIPAPIRLTRLGIIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCC
TPPALVEAAESFGATPWQVLRKVELPFAMPQIMAGLTQTIMLSLSMVVIAALVGADGLGV
CCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
PVLRALNTVNIARGFESGLCIVILAIVLDRLFRSSDEGEGA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]