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The map label for this gene is carA [H]

Identifier: 150397429

GI number: 150397429

Start: 2309639

End: 2310844

Strand: Direct

Name: carA [H]

Synonym: Smed_2229

Alternate gene names: 150397429

Gene position: 2309639-2310844 (Clockwise)

Preceding gene: 150397416

Following gene: 150397431

Centisome position: 61.07

GC content: 63.76

Gene sequence:

>1206_bases
ATGACCGCGACACCCGCATGGACAATCCAGAAACCCACTGCCCTGCTCGTTCTGGCCGACGGCACGGTGATCGAAGGCAA
GGGCATCGGCGCGACCGGAAAGGTTCAGGCCGAGGTCTGTTTCAACACGGCGCTGACCGGATACCAGGAAATCCTGACCG
ATCCCTCCTATCTCGGTCAGATCGTCACCTTCACCTTCCCGCATATCGGCAATATCGGCGCCAATGACGAGGACATCGAG
GATCTGACACCCGCCGCGCGCCACGGTGCCGTCGGCGTGATCTTCAAGGCCGACATCACGGAGCCCTCCAACTACCGCGC
CGCCAGGCATCTCGATACTTGGCTGAAGGCGCGCGGCATCATCGGCCTCTGCGGCATCGACACGCGTGCGCTGACCGCCT
GGATCCGCGAAAACGGCATGCCGAACGCCGTCATCGCACACGATCCGGCGGGCGTCTTCGATGTCGGGGCGTTAAAGGCC
GAGGCGAAGGCGTGGAGCGGTCTCGAAGGCCTCGACCTCGCCAAGGTCGCCACTTCCGGCCAGTCCTATCGCTGGGATGA
GAAGCCCTGGATCTGGGACGAGGGCTATTCGACGCTCGGCGAAACCGATGCCGCCTATCATGTCGTCGCCCTCGACTACG
GCGTCAAGCGGAACATTCTCCGCCTCTTCGCCGGGCTAAATTGCCGTGTCACCGTCGTCCCGGCTCAGACGAGCGCCGAG
GAAGTTCTGGCGCTAAGGCCCGATGGCATCTTCCTGTCGAACGGCCCGGGCGACCCGGCCGCAACCGGCGAATATGCCGT
GCCGGTCATCAAGGATCTCCTCAAGACGGATATCCCGGTCTTCGGCATATGCCTGGGCCACCAGATGCTGGCGCTGGCGC
TGGGTGCCAGGACCGAGAAGATGCACCAGGGCCACCACGGCGCCAACCACCCGGTCAAGGACCACACCACCGGCAAGGTC
GAGATCGTTTCGATGAATCACGGCTTCGCAGTCGATGCGAACTCGCTCCCGCAAGGGGTTGAACAGACTCACATCTCGCT
GTTCGACGGCACCAATTGCGGCCTGCGCGTCGACGGCAGGCCGGTCTTCTCGGTCCAGCACCACCCGGAAGCTTCGCCGG
GCCCGCAGGACAGCCATTACCTCTTCCGCCGCTTCCTGAACCTCATTCGTGAGAAGAAAGGCGAACCGGCACTCGCCGAG
CGCTGA

Upstream 100 bases:

>100_bases
CTCCCGCGGGGTCTCCGCGCGCCATGCCGCATGCGATATGAAATGGCCCTGAACCGACCGGCTTTCAAGCCTCGCTTTCG
GCCGCAACGGGATAGAGACG

Downstream 100 bases:

>100_bases
GCCGTTTTCAGGGCCCGCCTCGTGCGGGCCTTCTTGCCTCAGCCGTCGACGAGACGCTCAGCGATGAAATCGACTGTTGC
CCGCACGGAGGGAAGCTGCC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTATPAWTIQKPTALLVLADGTVIEGKGIGATGKVQAEVCFNTALTGYQEILTDPSYLGQIVTFTFPHIGNIGANDEDIE
DLTPAARHGAVGVIFKADITEPSNYRAARHLDTWLKARGIIGLCGIDTRALTAWIRENGMPNAVIAHDPAGVFDVGALKA
EAKAWSGLEGLDLAKVATSGQSYRWDEKPWIWDEGYSTLGETDAAYHVVALDYGVKRNILRLFAGLNCRVTVVPAQTSAE
EVLALRPDGIFLSNGPGDPAATGEYAVPVIKDLLKTDIPVFGICLGHQMLALALGARTEKMHQGHHGANHPVKDHTTGKV
EIVSMNHGFAVDANSLPQGVEQTHISLFDGTNCGLRVDGRPVFSVQHHPEASPGPQDSHYLFRRFLNLIREKKGEPALAE
R

Sequences:

>Translated_401_residues
MTATPAWTIQKPTALLVLADGTVIEGKGIGATGKVQAEVCFNTALTGYQEILTDPSYLGQIVTFTFPHIGNIGANDEDIE
DLTPAARHGAVGVIFKADITEPSNYRAARHLDTWLKARGIIGLCGIDTRALTAWIRENGMPNAVIAHDPAGVFDVGALKA
EAKAWSGLEGLDLAKVATSGQSYRWDEKPWIWDEGYSTLGETDAAYHVVALDYGVKRNILRLFAGLNCRVTVVPAQTSAE
EVLALRPDGIFLSNGPGDPAATGEYAVPVIKDLLKTDIPVFGICLGHQMLALALGARTEKMHQGHHGANHPVKDHTTGKV
EIVSMNHGFAVDANSLPQGVEQTHISLFDGTNCGLRVDGRPVFSVQHHPEASPGPQDSHYLFRRFLNLIREKKGEPALAE
R
>Mature_400_residues
TATPAWTIQKPTALLVLADGTVIEGKGIGATGKVQAEVCFNTALTGYQEILTDPSYLGQIVTFTFPHIGNIGANDEDIED
LTPAARHGAVGVIFKADITEPSNYRAARHLDTWLKARGIIGLCGIDTRALTAWIRENGMPNAVIAHDPAGVFDVGALKAE
AKAWSGLEGLDLAKVATSGQSYRWDEKPWIWDEGYSTLGETDAAYHVVALDYGVKRNILRLFAGLNCRVTVVPAQTSAEE
VLALRPDGIFLSNGPGDPAATGEYAVPVIKDLLKTDIPVFGICLGHQMLALALGARTEKMHQGHHGANHPVKDHTTGKVE
IVSMNHGFAVDANSLPQGVEQTHISLFDGTNCGLRVDGRPVFSVQHHPEASPGPQDSHYLFRRFLNLIREKKGEPALAER

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=389, Percent_Identity=37.0179948586118, Blast_Score=215, Evalue=7e-56,
Organism=Homo sapiens, GI21361331, Length=412, Percent_Identity=33.7378640776699, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI169790915, Length=412, Percent_Identity=33.7378640776699, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1786215, Length=383, Percent_Identity=51.9582245430809, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI193204318, Length=395, Percent_Identity=35.6962025316456, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324878, Length=392, Percent_Identity=35.969387755102, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6322331, Length=398, Percent_Identity=33.6683417085427, Blast_Score=205, Evalue=9e-54,
Organism=Drosophila melanogaster, GI45555749, Length=393, Percent_Identity=36.1323155216285, Blast_Score=195, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24642586, Length=393, Percent_Identity=36.1323155216285, Blast_Score=194, Evalue=6e-50,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 43186; Mature: 43055

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPAWTIQKPTALLVLADGTVIEGKGIGATGKVQAEVCFNTALTGYQEILTDPSYLGQ
CCCCCCEEEECCEEEEEEECCEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHCCHHHHCE
IVTFTFPHIGNIGANDEDIEDLTPAARHGAVGVIFKADITEPSNYRAARHLDTWLKARGI
EEEEECCCCCCCCCCCCCHHHCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCC
IGLCGIDTRALTAWIRENGMPNAVIAHDPAGVFDVGALKAEAKAWSGLEGLDLAKVATSG
EEEECCCHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCHHHHHCCC
QSYRWDEKPWIWDEGYSTLGETDAAYHVVALDYGVKRNILRLFAGLNCRVTVVPAQTSAE
CCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCC
EVLALRPDGIFLSNGPGDPAATGEYAVPVIKDLLKTDIPVFGICLGHQMLALALGARTEK
CEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHH
MHQGHHGANHPVKDHTTGKVEIVSMNHGFAVDANSLPQGVEQTHISLFDGTNCGLRVDGR
HHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCHHHCEEEEEECCCCCEEECCE
PVFSVQHHPEASPGPQDSHYLFRRFLNLIREKKGEPALAER
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TATPAWTIQKPTALLVLADGTVIEGKGIGATGKVQAEVCFNTALTGYQEILTDPSYLGQ
CCCCCEEEECCEEEEEEECCEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHCCHHHHCE
IVTFTFPHIGNIGANDEDIEDLTPAARHGAVGVIFKADITEPSNYRAARHLDTWLKARGI
EEEEECCCCCCCCCCCCCHHHCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCC
IGLCGIDTRALTAWIRENGMPNAVIAHDPAGVFDVGALKAEAKAWSGLEGLDLAKVATSG
EEEECCCHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCHHHHHCCC
QSYRWDEKPWIWDEGYSTLGETDAAYHVVALDYGVKRNILRLFAGLNCRVTVVPAQTSAE
CCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCCC
EVLALRPDGIFLSNGPGDPAATGEYAVPVIKDLLKTDIPVFGICLGHQMLALALGARTEK
CEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHH
MHQGHHGANHPVKDHTTGKVEIVSMNHGFAVDANSLPQGVEQTHISLFDGTNCGLRVDGR
HHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCHHHCEEEEEECCCCCEEECCE
PVFSVQHHPEASPGPQDSHYLFRRFLNLIREKKGEPALAER
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]