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The map label for this gene is metF [H]

Identifier: 150397316

GI number: 150397316

Start: 2177773

End: 2178714

Strand: Reverse

Name: metF [H]

Synonym: Smed_2115

Alternate gene names: 150397316

Gene position: 2178714-2177773 (Counterclockwise)

Preceding gene: 150397317

Following gene: 150397310

Centisome position: 57.61

GC content: 62.1

Gene sequence:

>942_bases
ATGAACGGACGGTTGCCCAATGCCGTGGACTGCCGCGGGATCAGATTTTCTTTCGAATACTTTCCTCCGAAGAATGACGA
GATGGAGGCTCAGCTGTTTCAAACCGTAACGGCTCTCTCCGTCTTCGCGCCCGCCTTCGTCACCGTCACCTACGGTGCCG
GCGGGTCGACCAAGGCGCGCTCCCTGACGACGGTTAAGCGGATGATCGGGGAGATGGCAATCGGGAATACGGCGGCGCAC
CTCACCTGTGTTGGCGCGCCGAAGGCGGATGTGGACGCGGTCGTCGACGAGTTCGTGGCTTGCGGAGTACGCCATTTCGT
GGCGTTGAGGGGCGACCCCCAAGGCGGGATCGGCAGCGTCTATGAGCCTTTTCCGGGCGGCTATGAAAATGCCGCGGACC
TCGTCAAGGCTCTGCGGGCACGAGGCGATTTCGAGATCTCTGTCTCGGCCTATCCTGAGAAGCATCCGGAAAGCTCCGAC
GTCGCTGCCGACATAGACATGCTGAAGCGTAAGGTCGACAAGGGCGCGACGCGTGCACTCACGCAGTTCTTCTTCGACAA
TGACGACTTCGAGCGCTATCTGGAACGCGTACGGCGCGCCGGCATCGGGATACCGGTCGTGCCGGGAATACTGCCGATCA
ACAACCTCGCCCAGGTGCAGAAATTCGCCGGACTTTGTGGCGCCAGGGTACCGGAGTCGCTTATTTCGCGGCTCGGTCCC
TTAAACGACCGACCGGAGGAGCGCTTCAGGGAGGCAGCCCATATCGCGGCCGAGCAGATTGCCGATCTCGTGCGGCGCGG
CGTCAACGACTTTCATCTCTATACGATGAACCGCCCGCAACTCGTCACGGCCGTTTGCGGATTGATCGGCTATGAAGCGG
AACGCGCGTCGGCTCAGCCGGTAGACACCGAGCGCTCGTTCCGGGCGGCCGCGACCGGGTGA

Upstream 100 bases:

>100_bases
GGAAACTGACTGTCACCATCTGGCTGGCGCGCGATCAGCGCGCGGTCGAATCCGTTGCCGACATGCCGCGCCAGCGCCTT
CATGCAGGGGGAGTTTGAGA

Downstream 100 bases:

>100_bases
CGTTTGCCGTCCTGCGAACTACGATGACCATGGAGAAAGCCCATGAACGCAGCGGCGTTCATGGGCTTTCTGTTGTTACA
GCCTGTCAATAAATGCCTGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNGRLPNAVDCRGIRFSFEYFPPKNDEMEAQLFQTVTALSVFAPAFVTVTYGAGGSTKARSLTTVKRMIGEMAIGNTAAH
LTCVGAPKADVDAVVDEFVACGVRHFVALRGDPQGGIGSVYEPFPGGYENAADLVKALRARGDFEISVSAYPEKHPESSD
VAADIDMLKRKVDKGATRALTQFFFDNDDFERYLERVRRAGIGIPVVPGILPINNLAQVQKFAGLCGARVPESLISRLGP
LNDRPEERFREAAHIAAEQIADLVRRGVNDFHLYTMNRPQLVTAVCGLIGYEAERASAQPVDTERSFRAAATG

Sequences:

>Translated_313_residues
MNGRLPNAVDCRGIRFSFEYFPPKNDEMEAQLFQTVTALSVFAPAFVTVTYGAGGSTKARSLTTVKRMIGEMAIGNTAAH
LTCVGAPKADVDAVVDEFVACGVRHFVALRGDPQGGIGSVYEPFPGGYENAADLVKALRARGDFEISVSAYPEKHPESSD
VAADIDMLKRKVDKGATRALTQFFFDNDDFERYLERVRRAGIGIPVVPGILPINNLAQVQKFAGLCGARVPESLISRLGP
LNDRPEERFREAAHIAAEQIADLVRRGVNDFHLYTMNRPQLVTAVCGLIGYEAERASAQPVDTERSFRAAATG
>Mature_313_residues
MNGRLPNAVDCRGIRFSFEYFPPKNDEMEAQLFQTVTALSVFAPAFVTVTYGAGGSTKARSLTTVKRMIGEMAIGNTAAH
LTCVGAPKADVDAVVDEFVACGVRHFVALRGDPQGGIGSVYEPFPGGYENAADLVKALRARGDFEISVSAYPEKHPESSD
VAADIDMLKRKVDKGATRALTQFFFDNDDFERYLERVRRAGIGIPVVPGILPINNLAQVQKFAGLCGARVPESLISRLGP
LNDRPEERFREAAHIAAEQIADLVRRGVNDFHLYTMNRPQLVTAVCGLIGYEAERASAQPVDTERSFRAAATG

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=286, Percent_Identity=31.4685314685315, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1790377, Length=277, Percent_Identity=41.5162454873646, Blast_Score=224, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI25147436, Length=312, Percent_Identity=28.2051282051282, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25147441, Length=312, Percent_Identity=28.2051282051282, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6321313, Length=296, Percent_Identity=31.0810810810811, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325234, Length=287, Percent_Identity=31.3588850174216, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24662658, Length=217, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33998; Mature: 33998

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGRLPNAVDCRGIRFSFEYFPPKNDEMEAQLFQTVTALSVFAPAFVTVTYGAGGSTKAR
CCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHH
SLTTVKRMIGEMAIGNTAAHLTCVGAPKADVDAVVDEFVACGVRHFVALRGDPQGGIGSV
HHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
YEPFPGGYENAADLVKALRARGDFEISVSAYPEKHPESSDVAADIDMLKRKVDKGATRAL
CCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TQFFFDNDDFERYLERVRRAGIGIPVVPGILPINNLAQVQKFAGLCGARVPESLISRLGP
HHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCC
LNDRPEERFREAAHIAAEQIADLVRRGVNDFHLYTMNRPQLVTAVCGLIGYEAERASAQP
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCHHHHCCCCC
VDTERSFRAAATG
CCCHHHCHHCCCC
>Mature Secondary Structure
MNGRLPNAVDCRGIRFSFEYFPPKNDEMEAQLFQTVTALSVFAPAFVTVTYGAGGSTKAR
CCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHH
SLTTVKRMIGEMAIGNTAAHLTCVGAPKADVDAVVDEFVACGVRHFVALRGDPQGGIGSV
HHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
YEPFPGGYENAADLVKALRARGDFEISVSAYPEKHPESSDVAADIDMLKRKVDKGATRAL
CCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TQFFFDNDDFERYLERVRRAGIGIPVVPGILPINNLAQVQKFAGLCGARVPESLISRLGP
HHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCC
LNDRPEERFREAAHIAAEQIADLVRRGVNDFHLYTMNRPQLVTAVCGLIGYEAERASAQP
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCHHHHCCCCC
VDTERSFRAAATG
CCCHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA