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The map label for this gene is murD [H]

Identifier: 150397285

GI number: 150397285

Start: 2141181

End: 2142572

Strand: Reverse

Name: murD [H]

Synonym: Smed_2084

Alternate gene names: 150397285

Gene position: 2142572-2141181 (Counterclockwise)

Preceding gene: 150397286

Following gene: 150397284

Centisome position: 56.65

GC content: 64.08

Gene sequence:

>1392_bases
ATGATCCCGGTCACTTCATTCAAGGGTAGGAAGGTCGCACTCTTCGGGCTGGGCGGCTCCGGACTGGCGACCGCCCAGGC
GCTCGTTGCAGGCGGAGCCGATGTGGTGGCTTGGGACGACAACCCCGACAGCGTCGCCAAGGCGGATCAGGCCGGGATCG
CGACGGCCGATCTGCGGGGCGAGGAATGGCATGCCTTTTCCGCCTTGGTCCTTTCGCCCGGCGTGCCGCTGACCCATCCA
AAGCCGCATTGGAGCGCCGACCTCGCGCATCATGCCGGCGTCGAGATCATCGGCGATGTCGAGCTGTTCGTGCGCGAGCG
GCGCAAGCACGCGCCTGACTGCCCTTTCATTGCCATCACCGGCACCAACGGCAAATCCACGACGACGGCGCTGATCGCCC
ATATCCTGCGCGCAAGTGGGCGGGACACACAGCTCGGCGGCAATATAGGCACAGCGGTGCTGACGCTGGAGCCGCCGCAG
GCGGACCGCTTCTATGTCGTCGAATGCTCATCCTACCAGATCGACCTGGCACCCACGCTCGATCCCACCGCCGGGATACT
CCTCAACCTCACGCCGGATCATCTGGATCGCCATGGTACGATGCAGCACTATGCCGACATCAAGGAGCGCCTGGTGGCGG
GGAGCGGAACGGCGATTGTCGGTGTCGACGACAGCCTTTCGAGTCTGATTGCCGACCGGGTGGAGCGAGCAGGTACCAAG
GTCGTGCGTATCTCGCGCCGTCATCCGCTTGCCGAAGGTGTCTATGCCGAAGGTACGGCGCTGATGCGTGCGACTGGCGG
GGCATCGTCGCTCTTTACCGACCTTGCCGGGATCCAGACGCTGCGTGGCGGTCACAATGCCCAGAATGCCGCGGCCGCGA
TCGCCGCGTGCCTGGCGGTCGGCATTTCCGAAAAGGACATAGTGGACGGCCTCAGAAGCTTTCCGGGGCTCAAGCACCGG
ATGCAGCCGGTTGCGAAGAAGGGCGAGACCATCTTCGTCAACGATAGCAAGGCGACCAACGCCGAGGCCGCAGCACCGGC
GCTGTCGAGTTACGACCGTATCTACTGGATCGCCGGCGGTCTGCCGAAGGAGGGCGGCATCACCTCGTTGACGCCATTCT
TTCCGAAAATCGTCAAAGCCTATCTGATCGGAGAGGCGGCGCCGTCTTTCGCGGCGACCCTCGGCGAGGCAGTGCCCTAC
GAAATCTCGGGGACATTGGAAAAAGCGGTTGCGCATGCGGCATCGGACGCGGCGCGCGATGCCGGGGCGCCGGCGACCGT
GATGCTTTCCCCGGCTTGCGCAAGCTTCGACCAGTATAAGAACTTCGAACTGCGCGGAGATGCCTTCGTCGAGCACGTGA
AGGCGCTCGAGGGCGTGATCATGCTCATCTGA

Upstream 100 bases:

>100_bases
AGAAGGGCTGGACGGAAAGCCAGGTGGTGATCCGCTTCTGGATCATCGCCGTGATCCTTGCGATGGTCGGTCTCTCGACG
CTGAAGCTTCGGTGAGCGCG

Downstream 100 bases:

>100_bases
CGGCTTCGGCCGGATAGTGACGTTGGCGCAGCACGCGCCGGAAAGGGGACAGAGAGATGGTAAGCCGAGCCGAACGGGGC
CCCGTGGCCGATTGGTTCTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MIPVTSFKGRKVALFGLGGSGLATAQALVAGGADVVAWDDNPDSVAKADQAGIATADLRGEEWHAFSALVLSPGVPLTHP
KPHWSADLAHHAGVEIIGDVELFVRERRKHAPDCPFIAITGTNGKSTTTALIAHILRASGRDTQLGGNIGTAVLTLEPPQ
ADRFYVVECSSYQIDLAPTLDPTAGILLNLTPDHLDRHGTMQHYADIKERLVAGSGTAIVGVDDSLSSLIADRVERAGTK
VVRISRRHPLAEGVYAEGTALMRATGGASSLFTDLAGIQTLRGGHNAQNAAAAIAACLAVGISEKDIVDGLRSFPGLKHR
MQPVAKKGETIFVNDSKATNAEAAAPALSSYDRIYWIAGGLPKEGGITSLTPFFPKIVKAYLIGEAAPSFAATLGEAVPY
EISGTLEKAVAHAASDAARDAGAPATVMLSPACASFDQYKNFELRGDAFVEHVKALEGVIMLI

Sequences:

>Translated_463_residues
MIPVTSFKGRKVALFGLGGSGLATAQALVAGGADVVAWDDNPDSVAKADQAGIATADLRGEEWHAFSALVLSPGVPLTHP
KPHWSADLAHHAGVEIIGDVELFVRERRKHAPDCPFIAITGTNGKSTTTALIAHILRASGRDTQLGGNIGTAVLTLEPPQ
ADRFYVVECSSYQIDLAPTLDPTAGILLNLTPDHLDRHGTMQHYADIKERLVAGSGTAIVGVDDSLSSLIADRVERAGTK
VVRISRRHPLAEGVYAEGTALMRATGGASSLFTDLAGIQTLRGGHNAQNAAAAIAACLAVGISEKDIVDGLRSFPGLKHR
MQPVAKKGETIFVNDSKATNAEAAAPALSSYDRIYWIAGGLPKEGGITSLTPFFPKIVKAYLIGEAAPSFAATLGEAVPY
EISGTLEKAVAHAASDAARDAGAPATVMLSPACASFDQYKNFELRGDAFVEHVKALEGVIMLI
>Mature_463_residues
MIPVTSFKGRKVALFGLGGSGLATAQALVAGGADVVAWDDNPDSVAKADQAGIATADLRGEEWHAFSALVLSPGVPLTHP
KPHWSADLAHHAGVEIIGDVELFVRERRKHAPDCPFIAITGTNGKSTTTALIAHILRASGRDTQLGGNIGTAVLTLEPPQ
ADRFYVVECSSYQIDLAPTLDPTAGILLNLTPDHLDRHGTMQHYADIKERLVAGSGTAIVGVDDSLSSLIADRVERAGTK
VVRISRRHPLAEGVYAEGTALMRATGGASSLFTDLAGIQTLRGGHNAQNAAAAIAACLAVGISEKDIVDGLRSFPGLKHR
MQPVAKKGETIFVNDSKATNAEAAAPALSSYDRIYWIAGGLPKEGGITSLTPFFPKIVKAYLIGEAAPSFAATLGEAVPY
EISGTLEKAVAHAASDAARDAGAPATVMLSPACASFDQYKNFELRGDAFVEHVKALEGVIMLI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=462, Percent_Identity=32.9004329004329, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48418; Mature: 48418

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVTSFKGRKVALFGLGGSGLATAQALVAGGADVVAWDDNPDSVAKADQAGIATADLRG
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCEEECCCC
EEWHAFSALVLSPGVPLTHPKPHWSADLAHHAGVEIIGDVELFVRERRKHAPDCPFIAIT
CCHHHEEEEEECCCCCCCCCCCCCCCCHHHHCCCEEEEHHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHILRASGRDTQLGGNIGTAVLTLEPPQADRFYVVECSSYQIDLAPTL
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPTAGILLNLTPDHLDRHGTMQHYADIKERLVAGSGTAIVGVDDSLSSLIADRVERAGTK
CCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCE
VVRISRRHPLAEGVYAEGTALMRATGGASSLFTDLAGIQTLRGGHNAQNAAAAIAACLAV
EEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GISEKDIVDGLRSFPGLKHRMQPVAKKGETIFVNDSKATNAEAAAPALSSYDRIYWIAGG
CCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHCHHHHCCCEEEEEECC
LPKEGGITSLTPFFPKIVKAYLIGEAAPSFAATLGEAVPYEISGTLEKAVAHAASDAARD
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHH
AGAPATVMLSPACASFDQYKNFELRGDAFVEHVKALEGVIMLI
CCCCEEEEECCHHHCHHHHCCCEECHHHHHHHHHHHCCEEEEC
>Mature Secondary Structure
MIPVTSFKGRKVALFGLGGSGLATAQALVAGGADVVAWDDNPDSVAKADQAGIATADLRG
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCEEECCCC
EEWHAFSALVLSPGVPLTHPKPHWSADLAHHAGVEIIGDVELFVRERRKHAPDCPFIAIT
CCHHHEEEEEECCCCCCCCCCCCCCCCHHHHCCCEEEEHHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHILRASGRDTQLGGNIGTAVLTLEPPQADRFYVVECSSYQIDLAPTL
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPTAGILLNLTPDHLDRHGTMQHYADIKERLVAGSGTAIVGVDDSLSSLIADRVERAGTK
CCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCE
VVRISRRHPLAEGVYAEGTALMRATGGASSLFTDLAGIQTLRGGHNAQNAAAAIAACLAV
EEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GISEKDIVDGLRSFPGLKHRMQPVAKKGETIFVNDSKATNAEAAAPALSSYDRIYWIAGG
CCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHCHHHHCCCEEEEEECC
LPKEGGITSLTPFFPKIVKAYLIGEAAPSFAATLGEAVPYEISGTLEKAVAHAASDAARD
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHH
AGAPATVMLSPACASFDQYKNFELRGDAFVEHVKALEGVIMLI
CCCCEEEEECCHHHCHHHHCCCEECHHHHHHHHHHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 7926844 [H]