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The map label for this gene is ddl [H]

Identifier: 150397278

GI number: 150397278

Start: 2132737

End: 2133663

Strand: Reverse

Name: ddl [H]

Synonym: Smed_2077

Alternate gene names: 150397278

Gene position: 2133663-2132737 (Counterclockwise)

Preceding gene: 150397279

Following gene: 150397277

Centisome position: 56.42

GC content: 59.44

Gene sequence:

>927_bases
ATGAGCAGCAAGCATGTCGCTGTCCTGTTGGGCGGATTTTCCTCGGAGAGGCCGGTGAGCCTGTCTTCGGGGACGGCTTG
CGCGGATGCTCTGGAAGGCGAAGGCTATCGCGTCACGCGCGTCGATGTCGGTCACGACGTCGCGGCGGTGCTTCAGGAAT
TGCGCCCGGATGTGGTCTTCAACGCGCTTCACGGACCATTCGGCGAAGACGGAACGATTCAGGGAATCCTCGAATATCTC
GAAATTCCCTATACCCATTCGGGCGTTCTTGCCTCTGCGCTGGCCATGGACAAGGCCCAGGCAAAGCATGTCGCCAAGGC
CGCCGGTATTCCGGTTGCCGAGGCAGTGGTCATGGACCGCCGCAGCTTCGGCAATCAGCATCCGATGAAGCCCCCCTACG
TGGTGAAGCCGGTGCGAGAGGGGTCGAGCTTCGGCGTGGTGATCGTCAAGGAGGATCAGTCGCATCCGCCTCAGGTGATC
ACCTCGAGCGACTGGCGCTACGGCGACCGCATCATGGTCGAGCGCTACGTTGCAGGACGCGAATTCACCTGCGGCGTCAT
GGGCGATGTCGCTCTTGGCGTCACTGAGATCATTCCGCAGGGGCATGCCTTCTACGATTACGACTCTAAATACGTAAAAG
GTGGCTCGAAGCACGTCATACCGGCACAGGTTTCACCAAATATTTACCAAAAAATACAAACACTGGCTTTGAAGGCGCAT
CAGGCGATCGGTTGCCGCGGCGTCAGCCGGTCCGACTTTCGTTTCGACGATCGTGGCGATGGTGAGGGCGAGTTGATCTG
GCTCGAGATCAACACCCAACCCGGCATGACCCCAACCTCCCTGGTGCCCGAAATGGCGCAGCATGCGGGCCTTCGGTTTG
GTGAATTTCTCAGGTGGATGGTGGAGGACGCGTCGTGCTTGCGTTGA

Upstream 100 bases:

>100_bases
CGTTGTAGTGGTTCTACGCGGCGATCTCGCGAATCGCGATTTCGGAATCAGCCAGGTGCAAGCAAGCTTCGCGATAATGG
CGTATTGTTTGGGGGTTTGA

Downstream 100 bases:

>100_bases
GGGGCAGAAGGGGCAAGAGGGTTCGTCACCCAGCCGGGGGTGTCGCCGAGGCGGATGAATCGTTCGTCCTGCCGCGACCG
CTGCGCAAGGGCGTGCGGTT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSSKHVAVLLGGFSSERPVSLSSGTACADALEGEGYRVTRVDVGHDVAAVLQELRPDVVFNALHGPFGEDGTIQGILEYL
EIPYTHSGVLASALAMDKAQAKHVAKAAGIPVAEAVVMDRRSFGNQHPMKPPYVVKPVREGSSFGVVIVKEDQSHPPQVI
TSSDWRYGDRIMVERYVAGREFTCGVMGDVALGVTEIIPQGHAFYDYDSKYVKGGSKHVIPAQVSPNIYQKIQTLALKAH
QAIGCRGVSRSDFRFDDRGDGEGELIWLEINTQPGMTPTSLVPEMAQHAGLRFGEFLRWMVEDASCLR

Sequences:

>Translated_308_residues
MSSKHVAVLLGGFSSERPVSLSSGTACADALEGEGYRVTRVDVGHDVAAVLQELRPDVVFNALHGPFGEDGTIQGILEYL
EIPYTHSGVLASALAMDKAQAKHVAKAAGIPVAEAVVMDRRSFGNQHPMKPPYVVKPVREGSSFGVVIVKEDQSHPPQVI
TSSDWRYGDRIMVERYVAGREFTCGVMGDVALGVTEIIPQGHAFYDYDSKYVKGGSKHVIPAQVSPNIYQKIQTLALKAH
QAIGCRGVSRSDFRFDDRGDGEGELIWLEINTQPGMTPTSLVPEMAQHAGLRFGEFLRWMVEDASCLR
>Mature_307_residues
SSKHVAVLLGGFSSERPVSLSSGTACADALEGEGYRVTRVDVGHDVAAVLQELRPDVVFNALHGPFGEDGTIQGILEYLE
IPYTHSGVLASALAMDKAQAKHVAKAAGIPVAEAVVMDRRSFGNQHPMKPPYVVKPVREGSSFGVVIVKEDQSHPPQVIT
SSDWRYGDRIMVERYVAGREFTCGVMGDVALGVTEIIPQGHAFYDYDSKYVKGGSKHVIPAQVSPNIYQKIQTLALKAHQ
AIGCRGVSRSDFRFDDRGDGEGELIWLEINTQPGMTPTSLVPEMAQHAGLRFGEFLRWMVEDASCLR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=311, Percent_Identity=37.620578778135, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=29.9719887955182, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33525; Mature: 33393

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKHVAVLLGGFSSERPVSLSSGTACADALEGEGYRVTRVDVGHDVAAVLQELRPDVVF
CCCCEEEEEEECCCCCCCEECCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHCHHHEE
NALHGPFGEDGTIQGILEYLEIPYTHSGVLASALAMDKAQAKHVAKAAGIPVAEAVVMDR
HHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RSFGNQHPMKPPYVVKPVREGSSFGVVIVKEDQSHPPQVITSSDWRYGDRIMVERYVAGR
HHCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCEEECCCCCCCCEEEEEHHHCCC
EFTCGVMGDVALGVTEIIPQGHAFYDYDSKYVKGGSKHVIPAQVSPNIYQKIQTLALKAH
CEEEEEHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHH
QAIGCRGVSRSDFRFDDRGDGEGELIWLEINTQPGMTPTSLVPEMAQHAGLRFGEFLRWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VEDASCLR
HHHHHHCC
>Mature Secondary Structure 
SSKHVAVLLGGFSSERPVSLSSGTACADALEGEGYRVTRVDVGHDVAAVLQELRPDVVF
CCCEEEEEEECCCCCCCEECCCCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHCHHHEE
NALHGPFGEDGTIQGILEYLEIPYTHSGVLASALAMDKAQAKHVAKAAGIPVAEAVVMDR
HHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RSFGNQHPMKPPYVVKPVREGSSFGVVIVKEDQSHPPQVITSSDWRYGDRIMVERYVAGR
HHCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCEEECCCCCCCCEEEEEHHHCCC
EFTCGVMGDVALGVTEIIPQGHAFYDYDSKYVKGGSKHVIPAQVSPNIYQKIQTLALKAH
CEEEEEHHHHHHHHHHHCCCCCEEECCCCHHHCCCCCEEEECCCCCHHHHHHHHHHHHHH
QAIGCRGVSRSDFRFDDRGDGEGELIWLEINTQPGMTPTSLVPEMAQHAGLRFGEFLRWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
VEDASCLR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]