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The map label for this gene is glcD [H]

Identifier: 150397232

GI number: 150397232

Start: 2078538

End: 2080016

Strand: Reverse

Name: glcD [H]

Synonym: Smed_2031

Alternate gene names: 150397232

Gene position: 2080016-2078538 (Counterclockwise)

Preceding gene: 150397234

Following gene: 150397231

Centisome position: 55.0

GC content: 61.93

Gene sequence:

>1479_bases
ATGTGGGTGGCCCAAGGGCGCCTCTTCTCGGTGCTGGCGAAGGTGCATCGGGAGACGAGAAGGAGAGTTTTGGTGGCATT
GAAGTCAATCGCGGCAGGGGTCAGGAACGAGACCGGCATTGCCGCGGCCAAGACCCACCTTGCTGCACGCTTCGGAGGCC
GGTTCCAGACCGGCGAAGCGATCCGGGCGCAGCACGCCCACACCACGACCTATATTCCCGCGCAGCTGCCCGACGCGGTC
GTTTTCCCCGAAAGTGCCGCCGAGGTGTGCGAGATCGTTGAAGTCGCCTGCGCGCATAGGGTGCCGCTCATTCCGTTTGG
AACGGGCTCTTCGCTCGAAGGTCACCTCAATGCGCCTCACGGCGGCATCTCGGTCGACATGATGCGGATGAACCGCGTAC
TCGCGGTCAGTGCCGAAGACCTGGACTGCACGGTGGAGCCGGGTGTGACGCGCGAGGAACTCAACACTTACCTGCGGGAT
ACGGGGCTCTTCTTTCCCATAGATCCGGGCGCGAACGCTTCGATCGGCGGCATGGCCTCGACGCGCGCCTCGGGCACCAA
CGCGGTTCGCTACGGCACGATGAAGGAAAACGTACTCGCCGTGACGGCAGTCGTGGCGGGCGGGCGCGAAATCAGGACGG
CTCATCGAGCGCGCAAATCGTCCGCCGGCTACGACCTGACACGGCTCTTCGTCGGTGCCGAAGGGACGCTCGGTATCATA
ACCTCGATCACGCTGCGCCTGCAGGGAATACCGGAGGTGATTTCCGGCGGCGTCTGCCCGTTTCCTACGATTGCCGACGC
CTGCAATGCCGTTATCCTGACCATCCAGTCCGGCATCCCGGTTGCCCGGATAGAGCTCCTGGATGCGTTGCAGATGAAGG
CGTGCAATGCCTATTCGGGTTTGAGCTACCAGGAAACACCGACGCTTTTCGTCGAATTCCACGGCGGTGGGCAAAGCGTC
GAGCTCCAGTCCCGCCAGTTTGCGGAAATAGCGTCGGAATTCGGCTCTCCCGGTTTCATCTGGACGACGAATCCAGAGGA
ACGGGCACGGCTCTGGAAGGCGCGGCACAATGCCTATTGGGCGCAGAAGAGCCTCATGCCCGGGCGAGCAATTCTCTCCA
CCGACGTTTGCGTGCCGATATCCAGGCTTGCCGACTGCGTCGCGGCGACGCACGAAGACATCGCGGCATATGGATTGGTC
GCACCGATCGTCGGACATGCCGGCGATGGCAACTTTCATGTAGGGCTGCTTTTCGATGACACGGATGCGGCTGACGTGGC
GCGGGCGGAGGCTTTCGTCGAGAGACTGAACGCGCGGGCGTTGTCCATGGATGGCACCTGCACGGGGGAACATGGGATCG
GCCAGGGCAAAATGCCGTTTCTAGCAGCCGAACTCGGTGATGCGCTTGATCTCATGCGGCAGATCAAACGCTCGCTCGAT
CCCGACAATATCTTCAATCCGGGCAAGATCTTTATCTGA

Upstream 100 bases:

>100_bases
CGATCAGGGCCCAAGAGAATGGAATGGGGATGGGAGCTTGCCTATTTTCCTTCCCGCCGGACCGGTTGCCCTTATTTCGA
GTGCGAATAAGGGATAGATT

Downstream 100 bases:

>100_bases
GGCTTGCAAAACCGGCTCAAAGCCGCAATGCAGAGGGCGAACGAACTGACGGGAATGTGACACGGTGCTGGCGCGAATAC
TGGTTACCCTCGGCGGACTG

Product: D-lactate dehydrogenase (cytochrome)

Products: (R)-acetoin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MWVAQGRLFSVLAKVHRETRRRVLVALKSIAAGVRNETGIAAAKTHLAARFGGRFQTGEAIRAQHAHTTTYIPAQLPDAV
VFPESAAEVCEIVEVACAHRVPLIPFGTGSSLEGHLNAPHGGISVDMMRMNRVLAVSAEDLDCTVEPGVTREELNTYLRD
TGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKENVLAVTAVVAGGREIRTAHRARKSSAGYDLTRLFVGAEGTLGII
TSITLRLQGIPEVISGGVCPFPTIADACNAVILTIQSGIPVARIELLDALQMKACNAYSGLSYQETPTLFVEFHGGGQSV
ELQSRQFAEIASEFGSPGFIWTTNPEERARLWKARHNAYWAQKSLMPGRAILSTDVCVPISRLADCVAATHEDIAAYGLV
APIVGHAGDGNFHVGLLFDDTDAADVARAEAFVERLNARALSMDGTCTGEHGIGQGKMPFLAAELGDALDLMRQIKRSLD
PDNIFNPGKIFI

Sequences:

>Translated_492_residues
MWVAQGRLFSVLAKVHRETRRRVLVALKSIAAGVRNETGIAAAKTHLAARFGGRFQTGEAIRAQHAHTTTYIPAQLPDAV
VFPESAAEVCEIVEVACAHRVPLIPFGTGSSLEGHLNAPHGGISVDMMRMNRVLAVSAEDLDCTVEPGVTREELNTYLRD
TGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKENVLAVTAVVAGGREIRTAHRARKSSAGYDLTRLFVGAEGTLGII
TSITLRLQGIPEVISGGVCPFPTIADACNAVILTIQSGIPVARIELLDALQMKACNAYSGLSYQETPTLFVEFHGGGQSV
ELQSRQFAEIASEFGSPGFIWTTNPEERARLWKARHNAYWAQKSLMPGRAILSTDVCVPISRLADCVAATHEDIAAYGLV
APIVGHAGDGNFHVGLLFDDTDAADVARAEAFVERLNARALSMDGTCTGEHGIGQGKMPFLAAELGDALDLMRQIKRSLD
PDNIFNPGKIFI
>Mature_492_residues
MWVAQGRLFSVLAKVHRETRRRVLVALKSIAAGVRNETGIAAAKTHLAARFGGRFQTGEAIRAQHAHTTTYIPAQLPDAV
VFPESAAEVCEIVEVACAHRVPLIPFGTGSSLEGHLNAPHGGISVDMMRMNRVLAVSAEDLDCTVEPGVTREELNTYLRD
TGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKENVLAVTAVVAGGREIRTAHRARKSSAGYDLTRLFVGAEGTLGII
TSITLRLQGIPEVISGGVCPFPTIADACNAVILTIQSGIPVARIELLDALQMKACNAYSGLSYQETPTLFVEFHGGGQSV
ELQSRQFAEIASEFGSPGFIWTTNPEERARLWKARHNAYWAQKSLMPGRAILSTDVCVPISRLADCVAATHEDIAAYGLV
APIVGHAGDGNFHVGLLFDDTDAADVARAEAFVERLNARALSMDGTCTGEHGIGQGKMPFLAAELGDALDLMRQIKRSLD
PDNIFNPGKIFI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=444, Percent_Identity=49.5495495495495, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI37595754, Length=467, Percent_Identity=47.1092077087794, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI119964728, Length=424, Percent_Identity=26.8867924528302, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI4501993, Length=464, Percent_Identity=25.4310344827586, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1789351, Length=418, Percent_Identity=28.2296650717703, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1787977, Length=507, Percent_Identity=27.810650887574, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI48994907, Length=175, Percent_Identity=29.1428571428571, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI48994914, Length=162, Percent_Identity=27.1604938271605, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71992373, Length=441, Percent_Identity=45.578231292517, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71992381, Length=441, Percent_Identity=44.2176870748299, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71992392, Length=376, Percent_Identity=47.8723404255319, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17534361, Length=425, Percent_Identity=24.4705882352941, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17556096, Length=459, Percent_Identity=25.4901960784314, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320027, Length=446, Percent_Identity=41.4798206278027, Blast_Score=317, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6320023, Length=431, Percent_Identity=25.2900232018561, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320764, Length=437, Percent_Identity=25.629290617849, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18921117, Length=425, Percent_Identity=27.5294117647059, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24639277, Length=425, Percent_Identity=27.5294117647059, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24639275, Length=425, Percent_Identity=27.5294117647059, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24653753, Length=461, Percent_Identity=26.4642082429501, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.4

Molecular weight: Translated: 52559; Mature: 52559

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWVAQGRLFSVLAKVHRETRRRVLVALKSIAAGVRNETGIAAAKTHLAARFGGRFQTGEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHH
IRAQHAHTTTYIPAQLPDAVVFPESAAEVCEIVEVACAHRVPLIPFGTGSSLEGHLNAPH
HHHHCCCCEEECCCCCCCCEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
GGISVDMMRMNRVLAVSAEDLDCTVEPGVTREELNTYLRDTGLFFPIDPGANASIGGMAS
CCCEEEHHHHCEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
TRASGTNAVRYGTMKENVLAVTAVVAGGREIRTAHRARKSSAGYDLTRLFVGAEGTLGII
CCCCCCCEEEECCCCCCCEEEEEEHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
TSITLRLQGIPEVISGGVCPFPTIADACNAVILTIQSGIPVARIELLDALQMKACNAYSG
EEEEEEECCCHHHHCCCCCCCCHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHCC
LSYQETPTLFVEFHGGGQSVELQSRQFAEIASEFGSPGFIWTTNPEERARLWKARHNAYW
CCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
AQKSLMPGRAILSTDVCVPISRLADCVAATHEDIAAYGLVAPIVGHAGDGNFHVGLLFDD
HHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
TDAADVARAEAFVERLNARALSMDGTCTGEHGIGQGKMPFLAAELGDALDLMRQIKRSLD
CCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PDNIFNPGKIFI
CCCCCCCCCEEC
>Mature Secondary Structure
MWVAQGRLFSVLAKVHRETRRRVLVALKSIAAGVRNETGIAAAKTHLAARFGGRFQTGEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHH
IRAQHAHTTTYIPAQLPDAVVFPESAAEVCEIVEVACAHRVPLIPFGTGSSLEGHLNAPH
HHHHCCCCEEECCCCCCCCEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
GGISVDMMRMNRVLAVSAEDLDCTVEPGVTREELNTYLRDTGLFFPIDPGANASIGGMAS
CCCEEEHHHHCEEEEEECCCCCEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
TRASGTNAVRYGTMKENVLAVTAVVAGGREIRTAHRARKSSAGYDLTRLFVGAEGTLGII
CCCCCCCEEEECCCCCCCEEEEEEHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
TSITLRLQGIPEVISGGVCPFPTIADACNAVILTIQSGIPVARIELLDALQMKACNAYSG
EEEEEEECCCHHHHCCCCCCCCHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHCC
LSYQETPTLFVEFHGGGQSVELQSRQFAEIASEFGSPGFIWTTNPEERARLWKARHNAYW
CCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
AQKSLMPGRAILSTDVCVPISRLADCVAATHEDIAAYGLVAPIVGHAGDGNFHVGLLFDD
HHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
TDAADVARAEAFVERLNARALSMDGTCTGEHGIGQGKMPFLAAELGDALDLMRQIKRSLD
CCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PDNIFNPGKIFI
CCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R,R)-butane-2,3-diol; NAD+

Specific reaction: (R,R)-butane-2,3-diol + NAD+ = (R)-acetoin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]