Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 150397222

GI number: 150397222

Start: 2070435

End: 2071229

Strand: Reverse

Name: thyA [H]

Synonym: Smed_2021

Alternate gene names: 150397222

Gene position: 2071229-2070435 (Counterclockwise)

Preceding gene: 150397223

Following gene: 150397221

Centisome position: 54.77

GC content: 57.61

Gene sequence:

>795_bases
ATGCGGCAATATCTCGATCTCCTCGAACATGTGATTGAAACGGGAACCGACCGGGGCGACCGGACAGGCACGGGTACGCG
CTCGGTTTTCGGCTACCAGATGCGCTTCGACCTGTCGCAGGGTTTCCCCGTGCTGACGACCAAGAAGCTGCATCTGCGCT
CGATCATCCATGAGCTTTTATGGTTCCTGAAAGGCGATACGAACATCGCCTATCTCAAGGAGCATGGCGTGCGCATATGG
GACGAATGGGCAGATGAGAACGGCGATCTTGGGCCGGTCTATGGCTACCAATGGCGGTCCTGGCCGACGCCGGACGGCGG
TCATATTGACCAGATCGCGACGCTCGTCGAAGGGTTGAAAACCAATCCGAATTCCCGCCGCCACATTGTTTCCGCCTGGA
ATCCGGCGCTTGTGGACGATATGGCGCTGCCGCCTTGCCATTGCCTCTTCCAGTTCTATGTGGCCGACGGAAAGCTCTCC
TGTCAGCTCTACCAGCGCTCGGGTGACATCTTTCTCGGCGTGCCCTTCAACATCGCTTCCTATGCGCTTCTGACGATGAT
GGTGGCGCAGGTGACGGGGCTTGAGGCCGGCGACTTCGTCCACACGCTCGGCGACGCTCATATCTACCGCAACCACTTCG
AACAGGCGCAGCTGCAGCTGACGAGAACGCCGAAGCCGCTGCCGAGAATGGAGATCAATCCAGCTGTGAAGGATCTATTT
TCATTTAGATTCGAAGACTTTGAACTCACCGGCTACGAAGCCGATTCACATATCAAGGCGCCGGTGGCGGTATAG

Upstream 100 bases:

>100_bases
CGCGTGCAGATTTCCGTTCCGGAAAACGACTTTGGGCCATTGGTTCGCGCGACGGTTCGTTCTATTTTCAAAACAACGAA
CGAATCGTCGGTTAAAATGC

Downstream 100 bases:

>100_bases
GTCGCGGTGACTTCAGCCCGGGTCCGCCCCGGATGACGAACGTGACCTGTCGCAGGTGGGCAGTCGTCGGAAGGCCGTCC
ATATTCGGCTCCTGGAAACG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRQYLDLLEHVIETGTDRGDRTGTGTRSVFGYQMRFDLSQGFPVLTTKKLHLRSIIHELLWFLKGDTNIAYLKEHGVRIW
DEWADENGDLGPVYGYQWRSWPTPDGGHIDQIATLVEGLKTNPNSRRHIVSAWNPALVDDMALPPCHCLFQFYVADGKLS
CQLYQRSGDIFLGVPFNIASYALLTMMVAQVTGLEAGDFVHTLGDAHIYRNHFEQAQLQLTRTPKPLPRMEINPAVKDLF
SFRFEDFELTGYEADSHIKAPVAV

Sequences:

>Translated_264_residues
MRQYLDLLEHVIETGTDRGDRTGTGTRSVFGYQMRFDLSQGFPVLTTKKLHLRSIIHELLWFLKGDTNIAYLKEHGVRIW
DEWADENGDLGPVYGYQWRSWPTPDGGHIDQIATLVEGLKTNPNSRRHIVSAWNPALVDDMALPPCHCLFQFYVADGKLS
CQLYQRSGDIFLGVPFNIASYALLTMMVAQVTGLEAGDFVHTLGDAHIYRNHFEQAQLQLTRTPKPLPRMEINPAVKDLF
SFRFEDFELTGYEADSHIKAPVAV
>Mature_264_residues
MRQYLDLLEHVIETGTDRGDRTGTGTRSVFGYQMRFDLSQGFPVLTTKKLHLRSIIHELLWFLKGDTNIAYLKEHGVRIW
DEWADENGDLGPVYGYQWRSWPTPDGGHIDQIATLVEGLKTNPNSRRHIVSAWNPALVDDMALPPCHCLFQFYVADGKLS
CQLYQRSGDIFLGVPFNIASYALLTMMVAQVTGLEAGDFVHTLGDAHIYRNHFEQAQLQLTRTPKPLPRMEINPAVKDLF
SFRFEDFELTGYEADSHIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=50, Blast_Score=288, Evalue=4e-78,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=66.6666666666667, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=44.8763250883392, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6324648, Length=291, Percent_Identity=41.9243986254296, Blast_Score=210, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=52.2968197879859, Blast_Score=291, Evalue=2e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30026; Mature: 30026

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQYLDLLEHVIETGTDRGDRTGTGTRSVFGYQMRFDLSQGFPVLTTKKLHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEECHHHHHHHHHHHHH
WFLKGDTNIAYLKEHGVRIWDEWADENGDLGPVYGYQWRSWPTPDGGHIDQIATLVEGLK
HHHCCCCCEEEEECCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
TNPNSRRHIVSAWNPALVDDMALPPCHCLFQFYVADGKLSCQLYQRSGDIFLGVPFNIAS
CCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTMMVAQVTGLEAGDFVHTLGDAHIYRNHFEQAQLQLTRTPKPLPRMEINPAVKDLF
HHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHH
SFRFEDFELTGYEADSHIKAPVAV
HHCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRQYLDLLEHVIETGTDRGDRTGTGTRSVFGYQMRFDLSQGFPVLTTKKLHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEECHHHHHHHHHHHHH
WFLKGDTNIAYLKEHGVRIWDEWADENGDLGPVYGYQWRSWPTPDGGHIDQIATLVEGLK
HHHCCCCCEEEEECCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
TNPNSRRHIVSAWNPALVDDMALPPCHCLFQFYVADGKLSCQLYQRSGDIFLGVPFNIAS
CCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTMMVAQVTGLEAGDFVHTLGDAHIYRNHFEQAQLQLTRTPKPLPRMEINPAVKDLF
HHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHH
SFRFEDFELTGYEADSHIKAPVAV
HHCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]