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The map label for this gene is dhfrIII [H]

Identifier: 150397220

GI number: 150397220

Start: 2069156

End: 2069719

Strand: Reverse

Name: dhfrIII [H]

Synonym: Smed_2019

Alternate gene names: 150397220

Gene position: 2069719-2069156 (Counterclockwise)

Preceding gene: 150397221

Following gene: 150397219

Centisome position: 54.73

GC content: 64.89

Gene sequence:

>564_bases
ATGACTGAAACAGAACGGAACCCGGCGATCTTACCGAAGATCGTTATCGTCGTCGCCGTCGCGTTGAACGGGGTCATCGG
CCGCGAGGGCGATCTCCCCTGGCGGCTGCCGACCGATCTCAAACGCTTCAAGGCACAAACGATCGGCAAGCCGGTCATCA
TGGGGCGGAAGACCTGGGCGGCAATCGGCAGGCCGCTGCCCGGCCGGCCGAACATCGTCGTCACCCGCAATCGCGCCTTC
GAGGCTCCCGGAGCCGAGGTCGCGCCCTCCCTCGAGGCGGCTCTTGCGGTCGCACGGGAGCGGGCGGCGGCGATTGGGGC
CGATGAGATTTGCGTTATCGGCGGCGGCGAGATCTACCGCCAGTCAATCGGCATGGCCGATGTCCTCCACGTTACCGAAG
TGCAGGCCGAAGTGGATGGCGACACACGCTTCCCCGAGATCGACCCGGCGGTTTTCGAAAAGGTGCTCGAAGAAGACCTG
CAAAGCGGCGAGAAAGACAGTCACGCTATGCATTTCGTGACCTGGCGGCGCCGTACTGCGCCGTCGCAGGAGACCGGGGC
CTGA

Upstream 100 bases:

>100_bases
CTGCCGGAGAGCGACGTGATAGACCGCTACATTCGGGTGGTCACCGACCGCGCCGCCCTGGTTCGCGCACGCGAGATCGA
CAGCAAGCCGAGTGGTTTTC

Downstream 100 bases:

>100_bases
GCGGTCTTCCGCTCTTGTCCACGTTCTCGCCCTTCGCTGCGGCACTCCGGTGAACAAATTTAACGCATTTACCGTGAACT
CGGCCGTATCGCGTTGAAAG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTETERNPAILPKIVIVVAVALNGVIGREGDLPWRLPTDLKRFKAQTIGKPVIMGRKTWAAIGRPLPGRPNIVVTRNRAF
EAPGAEVAPSLEAALAVARERAAAIGADEICVIGGGEIYRQSIGMADVLHVTEVQAEVDGDTRFPEIDPAVFEKVLEEDL
QSGEKDSHAMHFVTWRRRTAPSQETGA

Sequences:

>Translated_187_residues
MTETERNPAILPKIVIVVAVALNGVIGREGDLPWRLPTDLKRFKAQTIGKPVIMGRKTWAAIGRPLPGRPNIVVTRNRAF
EAPGAEVAPSLEAALAVARERAAAIGADEICVIGGGEIYRQSIGMADVLHVTEVQAEVDGDTRFPEIDPAVFEKVLEEDL
QSGEKDSHAMHFVTWRRRTAPSQETGA
>Mature_186_residues
TETERNPAILPKIVIVVAVALNGVIGREGDLPWRLPTDLKRFKAQTIGKPVIMGRKTWAAIGRPLPGRPNIVVTRNRAFE
APGAEVAPSLEAALAVARERAAAIGADEICVIGGGEIYRQSIGMADVLHVTEVQAEVDGDTRFPEIDPAVFEKVLEEDLQ
SGEKDSHAMHFVTWRRRTAPSQETGA

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=146, Percent_Identity=36.3013698630137, Blast_Score=80, Evalue=8e-16,
Organism=Homo sapiens, GI28827793, Length=146, Percent_Identity=36.3013698630137, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1786233, Length=165, Percent_Identity=41.8181818181818, Blast_Score=128, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17505899, Length=184, Percent_Identity=30.9782608695652, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324810, Length=167, Percent_Identity=31.7365269461078, Blast_Score=68, Evalue=7e-13,
Organism=Drosophila melanogaster, GI116008026, Length=171, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24647458, Length=171, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 20281; Mature: 20150

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETERNPAILPKIVIVVAVALNGVIGREGDLPWRLPTDLKRFKAQTIGKPVIMGRKTWA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHH
AIGRPLPGRPNIVVTRNRAFEAPGAEVAPSLEAALAVARERAAAIGADEICVIGGGEIYR
HHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHH
QSIGMADVLHVTEVQAEVDGDTRFPEIDPAVFEKVLEEDLQSGEKDSHAMHFVTWRRRTA
HHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHEEEHHCCC
PSQETGA
CCCCCCC
>Mature Secondary Structure 
TETERNPAILPKIVIVVAVALNGVIGREGDLPWRLPTDLKRFKAQTIGKPVIMGRKTWA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHH
AIGRPLPGRPNIVVTRNRAFEAPGAEVAPSLEAALAVARERAAAIGADEICVIGGGEIYR
HHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHH
QSIGMADVLHVTEVQAEVDGDTRFPEIDPAVFEKVLEEDLQSGEKDSHAMHFVTWRRRTA
HHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHEEEHHCCC
PSQETGA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]