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The map label for this gene is lysN [H]

Identifier: 150397157

GI number: 150397157

Start: 2006980

End: 2008206

Strand: Reverse

Name: lysN [H]

Synonym: Smed_1955

Alternate gene names: 150397157

Gene position: 2008206-2006980 (Counterclockwise)

Preceding gene: 150397159

Following gene: 150397154

Centisome position: 53.1

GC content: 62.75

Gene sequence:

>1227_bases
ATGCTGAACTGGGAAAGCATTTTTGCCACGCGCTCGAACCGGATGAGAGCCTCCGAAATCCGTGAGTTGCTGAAGCTGCT
GGATCGTCCCGAAATCATCTCGTTTGCCGGCGGTATTCCCGACCCGGAGCTTTTTCCGAACGATGCGTTCAAGGAAGCGT
ATGCCGAAATCTTCGGTGGCCCGACCGTCGGCGCAGCACTGCAATATTCGGTAAGCGAGGGCTATCGCCCGCTTCGCGAG
TGGCTCGCTGGTCAGATGGCGGCAATCGGCATTCCGGCGAGCGTCGACAATATCTTCATCACCTCCGGGTCGCAGCAGGG
GCTCGATTATCTCGGCAAACTGTTTCTGTCGCCGAAAGATACGGCGCTGGTGACCTGGCCGACCTATCTCGGTGCACTGC
AGGCCTTCAACGCCTACGAGCCGACCTACGATCAGCTGAACCCCGCCGGAAATCGCACGCCGGGGGCCTATGCCCAGGCG
GCCGAGGAAGGCGGTGGGCGCGTCAAGTTCGCCTATCTCTCGGCCGATTTCGCCAACCCGACCGGCGAGACGGTGGATCG
CGCCGGTCGCGAGCGTGTGCTCGCGCTTGCCGAGGAGCTCGACATCGCCGTCATCGAGGATGCGGCCTACCAGTCGCTGC
GCTATGACGGCGAGGCGGTTCCGCCGATCCTGGCGTTGGAGATCGCCCGCAAGGGCGACATAAACAACACCCGCACAATC
TATTGCGGCAGCTTCTCGAAGACGCTGGCGCCGGGCCTTCGCGTCGGCTGGATCTGTGCCGCCGAGCCGGTGATCCGCAA
GCTGGTGCTGATGAAGCAGGCCGCGGACCTTCACTCTTCGACGATCAATCAGATGGCGATCGCGACGGTCGCCGAGCGCG
GCTTCGATGAGCAGGTGAAGAAAATTCACGGGGCCTACAGGCAGCGCCGCAACGCCATGCTCTTGGCGCTCGAGAGATAC
ATGCCTGCTGGCGTCACCTGGACGAAGCCCGAAGGCGGCATGTTCATATGGGTAACCTTGCCAAAGGGCACCGATGGCGC
GGAGCTGCTCGCCAAATCGATCGAGACAGCCAAGGTGGCCTTTGTACCAGGCCGCGCCTTCTTCGCTGACGGCTCAGGTG
AAAACACGCTGCGGCTCAGCTTCTCCTGCGCCAACGGCAGGATGATCGACGAGGGCATCCGCCGACTCGGCGACCTCGTC
CGCGGCGAGGTTGCACAGGCGGCGTAA

Upstream 100 bases:

>100_bases
ACGGGTCAACCGCCGAATGAGAGCTGCCAGATAGGTCAGGAATATTGACATAAATCCAGCGCTGTGGTCCTCTTCCAAGA
ATGCAATCGGGAGGACCTCC

Downstream 100 bases:

>100_bases
GCAAGCTCATGAGGGGCGGCCGCGAATTCAGAGGTGCTGTCACTCACACATGCGGTCCGAGAAGCGAGAGGTCGGCTTTG
CCGAGCCGGTCCTTTGCGGT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLNWESIFATRSNRMRASEIRELLKLLDRPEIISFAGGIPDPELFPNDAFKEAYAEIFGGPTVGAALQYSVSEGYRPLRE
WLAGQMAAIGIPASVDNIFITSGSQQGLDYLGKLFLSPKDTALVTWPTYLGALQAFNAYEPTYDQLNPAGNRTPGAYAQA
AEEGGGRVKFAYLSADFANPTGETVDRAGRERVLALAEELDIAVIEDAAYQSLRYDGEAVPPILALEIARKGDINNTRTI
YCGSFSKTLAPGLRVGWICAAEPVIRKLVLMKQAADLHSSTINQMAIATVAERGFDEQVKKIHGAYRQRRNAMLLALERY
MPAGVTWTKPEGGMFIWVTLPKGTDGAELLAKSIETAKVAFVPGRAFFADGSGENTLRLSFSCANGRMIDEGIRRLGDLV
RGEVAQAA

Sequences:

>Translated_408_residues
MLNWESIFATRSNRMRASEIRELLKLLDRPEIISFAGGIPDPELFPNDAFKEAYAEIFGGPTVGAALQYSVSEGYRPLRE
WLAGQMAAIGIPASVDNIFITSGSQQGLDYLGKLFLSPKDTALVTWPTYLGALQAFNAYEPTYDQLNPAGNRTPGAYAQA
AEEGGGRVKFAYLSADFANPTGETVDRAGRERVLALAEELDIAVIEDAAYQSLRYDGEAVPPILALEIARKGDINNTRTI
YCGSFSKTLAPGLRVGWICAAEPVIRKLVLMKQAADLHSSTINQMAIATVAERGFDEQVKKIHGAYRQRRNAMLLALERY
MPAGVTWTKPEGGMFIWVTLPKGTDGAELLAKSIETAKVAFVPGRAFFADGSGENTLRLSFSCANGRMIDEGIRRLGDLV
RGEVAQAA
>Mature_408_residues
MLNWESIFATRSNRMRASEIRELLKLLDRPEIISFAGGIPDPELFPNDAFKEAYAEIFGGPTVGAALQYSVSEGYRPLRE
WLAGQMAAIGIPASVDNIFITSGSQQGLDYLGKLFLSPKDTALVTWPTYLGALQAFNAYEPTYDQLNPAGNRTPGAYAQA
AEEGGGRVKFAYLSADFANPTGETVDRAGRERVLALAEELDIAVIEDAAYQSLRYDGEAVPPILALEIARKGDINNTRTI
YCGSFSKTLAPGLRVGWICAAEPVIRKLVLMKQAADLHSSTINQMAIATVAERGFDEQVKKIHGAYRQRRNAMLLALERY
MPAGVTWTKPEGGMFIWVTLPKGTDGAELLAKSIETAKVAFVPGRAFFADGSGENTLRLSFSCANGRMIDEGIRRLGDLV
RGEVAQAA

Specific function: Catalyzes the transfer of an amino group between 2- oxoadipate (2-OA) and glutamate (Glu) to yield alpha-aminodipate (AAA). It can also transaminate glutamate, leucine, and aromatic amino acids. It also conbtributes in the biosynthesis of other amino acid

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7705897, Length=434, Percent_Identity=28.5714285714286, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI33469970, Length=434, Percent_Identity=28.5714285714286, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI95147551, Length=348, Percent_Identity=25, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI169881279, Length=348, Percent_Identity=25, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1787710, Length=385, Percent_Identity=27.2727272727273, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790797, Length=397, Percent_Identity=27.7078085642317, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1788722, Length=381, Percent_Identity=26.246719160105, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994949, Length=366, Percent_Identity=26.775956284153, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321000, Length=250, Percent_Identity=28.4, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321236, Length=492, Percent_Identity=21.5447154471545, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321929, Length=472, Percent_Identity=21.6101694915254, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356535, Length=421, Percent_Identity=25.1781472684086, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.39 [H]

Molecular weight: Translated: 44383; Mature: 44383

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNWESIFATRSNRMRASEIRELLKLLDRPEIISFAGGIPDPELFPNDAFKEAYAEIFGG
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCHHHHHHHHHHHCC
PTVGAALQYSVSEGYRPLREWLAGQMAAIGIPASVDNIFITSGSQQGLDYLGKLFLSPKD
CCCCHHEEEHHHHCCHHHHHHHCCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCC
TALVTWPTYLGALQAFNAYEPTYDQLNPAGNRTPGAYAQAAEEGGGRVKFAYLSADFANP
CEEEEHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCC
TGETVDRAGRERVLALAEELDIAVIEDAAYQSLRYDGEAVPPILALEIARKGDINNTRTI
CCHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHCCCCCCCCHHHEEEEHHCCCCCCCCEE
YCGSFSKTLAPGLRVGWICAAEPVIRKLVLMKQAADLHSSTINQMAIATVAERGFDEQVK
EECCCHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KIHGAYRQRRNAMLLALERYMPAGVTWTKPEGGMFIWVTLPKGTDGAELLAKSIETAKVA
HHHHHHHHHCCHHEEEHHHHCCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHEEEE
FVPGRAFFADGSGENTLRLSFSCANGRMIDEGIRRLGDLVRGEVAQAA
EECCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNWESIFATRSNRMRASEIRELLKLLDRPEIISFAGGIPDPELFPNDAFKEAYAEIFGG
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCHHHHHHHHHHHCC
PTVGAALQYSVSEGYRPLREWLAGQMAAIGIPASVDNIFITSGSQQGLDYLGKLFLSPKD
CCCCHHEEEHHHHCCHHHHHHHCCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCC
TALVTWPTYLGALQAFNAYEPTYDQLNPAGNRTPGAYAQAAEEGGGRVKFAYLSADFANP
CEEEEHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCC
TGETVDRAGRERVLALAEELDIAVIEDAAYQSLRYDGEAVPPILALEIARKGDINNTRTI
CCHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHCCCCCCCCHHHEEEEHHCCCCCCCCEE
YCGSFSKTLAPGLRVGWICAAEPVIRKLVLMKQAADLHSSTINQMAIATVAERGFDEQVK
EECCCHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KIHGAYRQRRNAMLLALERYMPAGVTWTKPEGGMFIWVTLPKGTDGAELLAKSIETAKVA
HHHHHHHHHCCHHEEEHHHHCCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHEEEE
FVPGRAFFADGSGENTLRLSFSCANGRMIDEGIRRLGDLVRGEVAQAA
EECCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA