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The map label for this gene is pgtE [H]

Identifier: 150396848

GI number: 150396848

Start: 1737035

End: 1738156

Strand: Direct

Name: pgtE [H]

Synonym: Smed_1643

Alternate gene names: 150396848

Gene position: 1737035-1738156 (Clockwise)

Preceding gene: 150396839

Following gene: 150396870

Centisome position: 45.93

GC content: 57.49

Gene sequence:

>1122_bases
ATGGTCTGCGAATACTTCCATTCCCTAAAGGCGTTGAAGCTGCCAGCCTCGCAACGGCAGGACGACACCGGTCCCTCAAC
ACCAGCGGCTTGCCAAGTGGGCAAGTCTGACACGAAGGGGATCCGAGGCGAAGAAGCTCAGGCTCAAGACGGAGGCGCTG
TACGAAAGACGCGGTCAGCTATGAAGCGCGTCTCCATCAGATCCGTAGCGATCTCCTGTTTTCTGTACGGTGCCCCATCA
TTCGCCGCCGCAGACAACGCTCTGTTTTCCTCCGACGATGGCAACGTCGTCGTTTTCGGCGATATCGGCCTCGCCAACAT
AAAGGCGCAGGAATTTTTCTATGTCGGCGACCACAAGATCAGCCAGTTGAACTGGGAGAGTAAAGGAGTCACTCTTTTCA
CGGTTGGCGTCGACGGGCAGATCGACAACGACTGGAGCTTGAAGGGCAGCGTCAAAGTCGGCACCGGCGGCAATGGTCAC
CTGGTTGACTACGACTGGACGATCATCGAGCGCGAAGACTGGAGCGACCGCTCAATCCACCCGCTTACCGAACTCGATCA
CTATGTCGCCGCAGCGATCGAGTTGGACCGGATCATCTACGGCAACGACACCAGCAGTATCGCGGTCGGCGCTGGTATGC
GCTATACCGACGTCAAATGGACCGCCTATGGCGGGTCAGGCATCTCTACGAAGAAAACGTTCCGCGATAAACGTTGGGAA
TTGCTAGACTGGGAAAGAGGCGCAAGCTACCGGCAAAAAATTCCGGTGGGCTTCCTCAGCCTGAGCGGCGAACACGTCCT
CGGCGATCTTACCATCAGCGGCGGCCTTCAGACCGGTCTGAGCTTCGGCATCAAGAGCATCGACGACCATTGGCTGCGCA
ACCTGCGTTTTTCGGATGACATGTCTCCGGCACCGACAATCGGCGCCAATGTCGCCGTCAGCTATGCGGTGACGCCCGGT
GCTTCGCTTTATCTGTCCGGTTCGTTCGAGCGGGTGTTTCACAGCCGCGGGGACAAGGAACGTCACAACTTCGCGACAGG
CGAAATTGAATTCCGCAAGGATTATGCCGGGGCCACGTTCGAGGCAATGTCAGTCTCCTTCGGGCTAAAGGGCACGTTTT
GA

Upstream 100 bases:

>100_bases
GCACGCGCGGCGCAGCTATCAATTATCCATTGGCAGGCCTAATGGCCGCGCTCTCCACTGTCTCCGCGAATCGATCACTC
TCCTAGCAAAGGACAACGAA

Downstream 100 bases:

>100_bases
CGTGCCCGCGTTGCCGCTATCTTTATCAGAAGACCATCGCCGCGCTCTTGCCAGTCTGACAATCCCGGACACAACAGCTC
TGGAAAATACGGTCGGGCCG

Product: outer membrane protease

Products: Hydrolyzed protein [C]

Alternate protein names: E protein [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MVCEYFHSLKALKLPASQRQDDTGPSTPAACQVGKSDTKGIRGEEAQAQDGGAVRKTRSAMKRVSIRSVAISCFLYGAPS
FAAADNALFSSDDGNVVVFGDIGLANIKAQEFFYVGDHKISQLNWESKGVTLFTVGVDGQIDNDWSLKGSVKVGTGGNGH
LVDYDWTIIEREDWSDRSIHPLTELDHYVAAAIELDRIIYGNDTSSIAVGAGMRYTDVKWTAYGGSGISTKKTFRDKRWE
LLDWERGASYRQKIPVGFLSLSGEHVLGDLTISGGLQTGLSFGIKSIDDHWLRNLRFSDDMSPAPTIGANVAVSYAVTPG
ASLYLSGSFERVFHSRGDKERHNFATGEIEFRKDYAGATFEAMSVSFGLKGTF

Sequences:

>Translated_373_residues
MVCEYFHSLKALKLPASQRQDDTGPSTPAACQVGKSDTKGIRGEEAQAQDGGAVRKTRSAMKRVSIRSVAISCFLYGAPS
FAAADNALFSSDDGNVVVFGDIGLANIKAQEFFYVGDHKISQLNWESKGVTLFTVGVDGQIDNDWSLKGSVKVGTGGNGH
LVDYDWTIIEREDWSDRSIHPLTELDHYVAAAIELDRIIYGNDTSSIAVGAGMRYTDVKWTAYGGSGISTKKTFRDKRWE
LLDWERGASYRQKIPVGFLSLSGEHVLGDLTISGGLQTGLSFGIKSIDDHWLRNLRFSDDMSPAPTIGANVAVSYAVTPG
ASLYLSGSFERVFHSRGDKERHNFATGEIEFRKDYAGATFEAMSVSFGLKGTF
>Mature_373_residues
MVCEYFHSLKALKLPASQRQDDTGPSTPAACQVGKSDTKGIRGEEAQAQDGGAVRKTRSAMKRVSIRSVAISCFLYGAPS
FAAADNALFSSDDGNVVVFGDIGLANIKAQEFFYVGDHKISQLNWESKGVTLFTVGVDGQIDNDWSLKGSVKVGTGGNGH
LVDYDWTIIEREDWSDRSIHPLTELDHYVAAAIELDRIIYGNDTSSIAVGAGMRYTDVKWTAYGGSGISTKKTFRDKRWE
LLDWERGASYRQKIPVGFLSLSGEHVLGDLTISGGLQTGLSFGIKSIDDHWLRNLRFSDDMSPAPTIGANVAVSYAVTPG
ASLYLSGSFERVFHSRGDKERHNFATGEIEFRKDYAGATFEAMSVSFGLKGTF

Specific function: Protease that can cleave T7 RNA polymerase. Specific for paired basic residues [H]

COG id: COG4571

COG function: function code M; Outer membrane protease

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A26 family [H]

Homologues:

Organism=Escherichia coli, GI1786777, Length=310, Percent_Identity=24.8387096774194, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020080
- InterPro:   IPR000036
- InterPro:   IPR020079 [H]

Pfam domain/function: PF01278 Omptin [H]

EC number: 3.4.21.87 [C]

Molecular weight: Translated: 40647; Mature: 40647

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCEYFHSLKALKLPASQRQDDTGPSTPAACQVGKSDTKGIRGEEAQAQDGGAVRKTRSA
CCCHHHHHHHEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
MKRVSIRSVAISCFLYGAPSFAAADNALFSSDDGNVVVFGDIGLANIKAQEFFYVGDHKI
HHHHHHHEEEEEEEEECCCCHHCCCCCEEECCCCCEEEEECCCCEEEEEEEEEEECCCEE
SQLNWESKGVTLFTVGVDGQIDNDWSLKGSVKVGTGGNGHLVDYDWTIIEREDWSDRSIH
EEECCCCCCEEEEEEECCCEECCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCCCCCCC
PLTELDHYVAAAIELDRIIYGNDTSSIAVGAGMRYTDVKWTAYGGSGISTKKTFRDKRWE
CHHHHHHHHHHHEEEEEEEECCCCCCEEEECCCEEEEEEEEEECCCCCCCHHHHHCCCCC
LLDWERGASYRQKIPVGFLSLSGEHVLGDLTISGGLQTGLSFGIKSIDDHWLRNLRFSDD
EEECCCCCCHHHCCCEEEEEECCCEEEEEEEECCCHHHHHHHCCCHHCHHHHHCCCCCCC
MSPAPTIGANVAVSYAVTPGASLYLSGSFERVFHSRGDKERHNFATGEIEFRKDYAGATF
CCCCCCCCCCEEEEEEECCCCEEEECCCHHHHHHCCCCCHHHCCCCCEEEEEECCCCCEE
EAMSVSFGLKGTF
EEEEEEECCCCCC
>Mature Secondary Structure
MVCEYFHSLKALKLPASQRQDDTGPSTPAACQVGKSDTKGIRGEEAQAQDGGAVRKTRSA
CCCHHHHHHHEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
MKRVSIRSVAISCFLYGAPSFAAADNALFSSDDGNVVVFGDIGLANIKAQEFFYVGDHKI
HHHHHHHEEEEEEEEECCCCHHCCCCCEEECCCCCEEEEECCCCEEEEEEEEEEECCCEE
SQLNWESKGVTLFTVGVDGQIDNDWSLKGSVKVGTGGNGHLVDYDWTIIEREDWSDRSIH
EEECCCCCCEEEEEEECCCEECCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCCCCCCC
PLTELDHYVAAAIELDRIIYGNDTSSIAVGAGMRYTDVKWTAYGGSGISTKKTFRDKRWE
CHHHHHHHHHHHEEEEEEEECCCCCCEEEECCCEEEEEEEEEECCCCCCCHHHHHCCCCC
LLDWERGASYRQKIPVGFLSLSGEHVLGDLTISGGLQTGLSFGIKSIDDHWLRNLRFSDD
EEECCCCCCHHHCCCEEEEEECCCEEEEEEEECCCHHHHHHHCCCHHCHHHHHCCCCCCC
MSPAPTIGANVAVSYAVTPGASLYLSGSFERVFHSRGDKERHNFATGEIEFRKDYAGATF
CCCCCCCCCCEEEEEEECCCCEEEECCCHHHHHHCCCCCHHHCCCCCEEEEEECCCCCEE
EAMSVSFGLKGTF
EEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: EDTA; Tosyl-L-Lys chloromethyl ketone [C]

Metal ions: NA

Kcat value (1/min): 40 [C]

Specific activity: 58.7

Km value (mM): 0.0036 {plasminogen}} 0.000058 {plasminogen}} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Arginine; Benzamidine; Co2+; Cu2+; Diisopropyl fluorophosphate; Fe2+; NaCl; Tosyl -L-phenyl alaninechloromethyl ketone; Zn2+ [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3023201; 10869088; 11677609; 2651422 [H]