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The map label for this gene is gph [H]

Identifier: 150396783

GI number: 150396783

Start: 1671545

End: 1672228

Strand: Direct

Name: gph [H]

Synonym: Smed_1574

Alternate gene names: 150396783

Gene position: 1671545-1672228 (Clockwise)

Preceding gene: 150396777

Following gene: 150396785

Centisome position: 44.2

GC content: 62.57

Gene sequence:

>684_bases
GTGTCCTTGCCCCTAGTCGTCTTCGATCTCGACGGTACGCTCGTCGACACAGCGCCCGACCTCGTCGCCAGCTTGAACCA
TGCCGTGACGCAGGTTGGCCTCGAGCCAGTGACCTACGCGGATCTCACGCATCTGGTCGGCCACGGCGCACGGGCGATGA
TCGAAAGGACTTTCGCACTCCGCGGCAGGACGATCGCCGAAGACGAACTGACCCGGCAGATGAACGTGTTCGTCGAGTTC
TATCATGGATCGATGCCGGGCGAATCCCTGCCCTACCCCGGTCTCGTCGAGGCCCTCGACCGCCTGCAGGATGCGGGCAT
GAAACTTGCCGTCTGCACCAACAAGCCGGAAATGCTGGCAAAACGCCTTCTGGAGGGGCTTGGACTCCTCGACCGCTTTG
CGGCGATATCCGGTGGCGATACCTTCTCCGTTCGCAAACCGCATGCCGACCACCTGTTGTCGACGGTCGCGAATGCCCGT
GGCCTGCCCGAGCGGGCGGTGATGGTGGGCGACAGCCTCAACGACATCCTGGTCGCCCGAAATGCCGCAGTTCCTTCGAT
CGTCGTGCCGTTCGGCTATTCCGACGTGCCGATCGAAAGCCTCCTTCCCGACCGCATCATCGGTCATTTCGACGAACTGA
CGCCGGATCTGGTCGAGGCAGTGCTCTCGCGCAAGCCGAAATGA

Upstream 100 bases:

>100_bases
TGCATTGGATTCCGGCAGCCCTACGACCTAGGTCGCCATTGCATTCCGCGCCCGGTTCGCCTACCCGAACGGAGATGTTT
TTCGCAGAATGAGGTTGCCG

Downstream 100 bases:

>100_bases
TACGTGCCCGCAGTTTAGAAGGGCCCGCTTTCCTCACCCTTCCGCGTGCCGCGCCTTGCGCGTCGCCTCGAAGGCGCGGT
TCACGTACATGTCGCGCTCG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSLPLVVFDLDGTLVDTAPDLVASLNHAVTQVGLEPVTYADLTHLVGHGARAMIERTFALRGRTIAEDELTRQMNVFVEF
YHGSMPGESLPYPGLVEALDRLQDAGMKLAVCTNKPEMLAKRLLEGLGLLDRFAAISGGDTFSVRKPHADHLLSTVANAR
GLPERAVMVGDSLNDILVARNAAVPSIVVPFGYSDVPIESLLPDRIIGHFDELTPDLVEAVLSRKPK

Sequences:

>Translated_227_residues
MSLPLVVFDLDGTLVDTAPDLVASLNHAVTQVGLEPVTYADLTHLVGHGARAMIERTFALRGRTIAEDELTRQMNVFVEF
YHGSMPGESLPYPGLVEALDRLQDAGMKLAVCTNKPEMLAKRLLEGLGLLDRFAAISGGDTFSVRKPHADHLLSTVANAR
GLPERAVMVGDSLNDILVARNAAVPSIVVPFGYSDVPIESLLPDRIIGHFDELTPDLVEAVLSRKPK
>Mature_226_residues
SLPLVVFDLDGTLVDTAPDLVASLNHAVTQVGLEPVTYADLTHLVGHGARAMIERTFALRGRTIAEDELTRQMNVFVEFY
HGSMPGESLPYPGLVEALDRLQDAGMKLAVCTNKPEMLAKRLLEGLGLLDRFAAISGGDTFSVRKPHADHLLSTVANARG
LPERAVMVGDSLNDILVARNAAVPSIVVPFGYSDVPIESLLPDRIIGHFDELTPDLVEAVLSRKPK

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=231, Percent_Identity=33.7662337662338, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24545; Mature: 24413

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPLVVFDLDGTLVDTAPDLVASLNHAVTQVGLEPVTYADLTHLVGHGARAMIERTFAL
CCCCEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHH
RGRTIAEDELTRQMNVFVEFYHGSMPGESLPYPGLVEALDRLQDAGMKLAVCTNKPEMLA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHH
KRLLEGLGLLDRFAAISGGDTFSVRKPHADHLLSTVANARGLPERAVMVGDSLNDILVAR
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHEEECCCHHHHHHCC
NAAVPSIVVPFGYSDVPIESLLPDRIIGHFDELTPDLVEAVLSRKPK
CCCCCEEEEECCCCCCCHHHHCHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SLPLVVFDLDGTLVDTAPDLVASLNHAVTQVGLEPVTYADLTHLVGHGARAMIERTFAL
CCCEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHH
RGRTIAEDELTRQMNVFVEFYHGSMPGESLPYPGLVEALDRLQDAGMKLAVCTNKPEMLA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHH
KRLLEGLGLLDRFAAISGGDTFSVRKPHADHLLSTVANARGLPERAVMVGDSLNDILVAR
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCHHHEEECCCHHHHHHCC
NAAVPSIVVPFGYSDVPIESLLPDRIIGHFDELTPDLVEAVLSRKPK
CCCCCEEEEECCCCCCCHHHHCHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]