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The map label for this gene is purC [H]

Identifier: 150396710

GI number: 150396710

Start: 1581234

End: 1581998

Strand: Direct

Name: purC [H]

Synonym: Smed_1498

Alternate gene names: 150396710

Gene position: 1581234-1581998 (Clockwise)

Preceding gene: 150396708

Following gene: 150396711

Centisome position: 41.81

GC content: 58.17

Gene sequence:

>765_bases
ATGAATCGTCGCCGCCGTATCTACGAGGGCAAGGCCAAGATCCTTTACGAGGGCCCGGAACCGGGTACGCTGATCCAGTT
TTTCAAGGACGACGCCACCGCCTTCAACAAGAAGAAACATGACGTCATCGACGGCAAGGGCGTGCTCAACAACCGCATTT
CCGAGTACATTTTCACCCATCTGAACAAGATCGGCATTCCGACGCATTTCATCCGCCGCCTCAACATGCGTGAGCAGTTG
ATCAAGGAAGTGGAGATCATCCCGCTCGAGATCGTCGTGCGCAATGTTGCTGCCGGCTCTCTCTCCAAGCGCCTTGGAAT
CGAGGAAGGCACAGTGCTGCCGCGCTCGATCATCGAGTTCTATTACAAGGCCGACGCGCTTGACGATCCGATGGTCTCCG
AAGAGCACATCACAGCTTTCGGCTGGGCGAGCCCGCAGGAACTCGACGACATCATGGCGCTCGCCATCCGCATCAACGAT
TTCCTTTCCGGCCTCTTCCTCGGCGTCGGCATCCAGCTCGTCGATTTCAAGATCGAATGCGGCCGCCTCTACGAGGGCGA
CATGATGCGCATCATCCTTGCCGACGAGATTTCCCCCGACAGCTGCCGTCTCTGGGACATCGAGACGAAAGAAAAGATGG
ACAAGGACCGCTTCCGCCGCGATATGGGCGGTCTCGTCGAAGCCTATCAGGAAGTGGCCCGCCGCCTCGGCATCATCAAC
GAAAACGAGCCGCCGCGCGGTTCGGGTCCGGTGCTGGTCAAGTAA

Upstream 100 bases:

>100_bases
AGACAGGCCGATTGTTAATTGTCTTCTTTTCGGATATGGACCCGCTGAGAAAATTCCCAATGCGCCTCCCAAGAGCGCCA
ACAACCACAGAGACAAATCC

Downstream 100 bases:

>100_bases
TCTCGAGGGTAAGTCAAAGTGATCAAGGCACGCGTAACCGTGACGCTTAAGAACGGTGTTCTCGACCCGCAGGGCAAGGC
GATCGAAGGCGCGCTCGGCG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNRRRRIYEGKAKILYEGPEPGTLIQFFKDDATAFNKKKHDVIDGKGVLNNRISEYIFTHLNKIGIPTHFIRRLNMREQL
IKEVEIIPLEIVVRNVAAGSLSKRLGIEEGTVLPRSIIEFYYKADALDDPMVSEEHITAFGWASPQELDDIMALAIRIND
FLSGLFLGVGIQLVDFKIECGRLYEGDMMRIILADEISPDSCRLWDIETKEKMDKDRFRRDMGGLVEAYQEVARRLGIIN
ENEPPRGSGPVLVK

Sequences:

>Translated_254_residues
MNRRRRIYEGKAKILYEGPEPGTLIQFFKDDATAFNKKKHDVIDGKGVLNNRISEYIFTHLNKIGIPTHFIRRLNMREQL
IKEVEIIPLEIVVRNVAAGSLSKRLGIEEGTVLPRSIIEFYYKADALDDPMVSEEHITAFGWASPQELDDIMALAIRIND
FLSGLFLGVGIQLVDFKIECGRLYEGDMMRIILADEISPDSCRLWDIETKEKMDKDRFRRDMGGLVEAYQEVARRLGIIN
ENEPPRGSGPVLVK
>Mature_254_residues
MNRRRRIYEGKAKILYEGPEPGTLIQFFKDDATAFNKKKHDVIDGKGVLNNRISEYIFTHLNKIGIPTHFIRRLNMREQL
IKEVEIIPLEIVVRNVAAGSLSKRLGIEEGTVLPRSIIEFYYKADALDDPMVSEEHITAFGWASPQELDDIMALAIRIND
FLSGLFLGVGIQLVDFKIECGRLYEGDMMRIILADEISPDSCRLWDIETKEKMDKDRFRRDMGGLVEAYQEVARRLGIIN
ENEPPRGSGPVLVK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=228, Percent_Identity=35.5263157894737, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI5453539, Length=228, Percent_Identity=35.5263157894737, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI119220559, Length=235, Percent_Identity=34.0425531914894, Blast_Score=101, Evalue=6e-22,
Organism=Escherichia coli, GI1788820, Length=238, Percent_Identity=46.6386554621849, Blast_Score=217, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17531275, Length=231, Percent_Identity=31.1688311688312, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI18860083, Length=237, Percent_Identity=30.8016877637131, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583917, Length=250, Percent_Identity=24, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 29052; Mature: 29052

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS01057 SAICAR_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRRRIYEGKAKILYEGPEPGTLIQFFKDDATAFNKKKHDVIDGKGVLNNRISEYIFTH
CCCCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LNKIGIPTHFIRRLNMREQLIKEVEIIPLEIVVRNVAAGSLSKRLGIEEGTVLPRSIIEF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
YYKADALDDPMVSEEHITAFGWASPQELDDIMALAIRINDFLSGLFLGVGIQLVDFKIEC
HHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEC
GRLYEGDMMRIILADEISPDSCRLWDIETKEKMDKDRFRRDMGGLVEAYQEVARRLGIIN
CCEECCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENEPPRGSGPVLVK
CCCCCCCCCCEEEC
>Mature Secondary Structure
MNRRRRIYEGKAKILYEGPEPGTLIQFFKDDATAFNKKKHDVIDGKGVLNNRISEYIFTH
CCCCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LNKIGIPTHFIRRLNMREQLIKEVEIIPLEIVVRNVAAGSLSKRLGIEEGTVLPRSIIEF
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
YYKADALDDPMVSEEHITAFGWASPQELDDIMALAIRINDFLSGLFLGVGIQLVDFKIEC
HHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEC
GRLYEGDMMRIILADEISPDSCRLWDIETKEKMDKDRFRRDMGGLVEAYQEVARRLGIIN
CCEECCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENEPPRGSGPVLVK
CCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA