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The map label for this gene is gloA [H]

Identifier: 150396663

GI number: 150396663

Start: 1529526

End: 1529966

Strand: Direct

Name: gloA [H]

Synonym: Smed_1450

Alternate gene names: 150396663

Gene position: 1529526-1529966 (Clockwise)

Preceding gene: 150396659

Following gene: 150396664

Centisome position: 40.44

GC content: 59.41

Gene sequence:

>441_bases
ATGCGGTATCTGCATACGATGGTTCGCGTCAACGACCTCGACAAGGCCCTTCATTTCTATTGCAAGCTTTTCGGGCTCCA
GGAGATTCGCCGATACGAGAACGAGAAAGGCCGCTTCACGCTCGTTTTCCTCGCCGCTCCCGGCGATATCGATCGCGCCA
AAAGCGAAGCCGCGCCATGCCTGGAACTCACCTATAACTGGGACCCGGAGGAGTATACCGGCGGGCGGAATTTCGGCCAC
CTCGCCTATGAGGTCGACGATATCTATGCGTTCTGCAAGCACCTGATGGATGGTGGCGTTACGATCAACCGTCCGCCTCG
CGACGGCCACATGGCTTTCGTGCGGTCGCCCGACGGCATCTCCATCGAGATCCTGCAGAAAGGCGAGCGCCTTCCCGCCC
AGGAACCCTGGGCCTCCATGGCCAACACAGGCGAATGGTAA

Upstream 100 bases:

>100_bases
GACGTATTTTGGTCTGAGACGTTTATAGGGACGCAGCCTTGCGTCTGGCTTGCCGCCGTTTCACTATACGCCATCTTTCA
CCAAACAGGGACATTCTCCA

Downstream 100 bases:

>100_bases
GTACGGGCGCGTGGTAAGCCTCGCGCAATTGCCCCCGGTTACACCTTAGACGCCGCCGGTCATGCTGAGCGGCGCGGGCG
GCGCCGGTGCAAAAGGATCG

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MRYLHTMVRVNDLDKALHFYCKLFGLQEIRRYENEKGRFTLVFLAAPGDIDRAKSEAAPCLELTYNWDPEEYTGGRNFGH
LAYEVDDIYAFCKHLMDGGVTINRPPRDGHMAFVRSPDGISIEILQKGERLPAQEPWASMANTGEW

Sequences:

>Translated_146_residues
MRYLHTMVRVNDLDKALHFYCKLFGLQEIRRYENEKGRFTLVFLAAPGDIDRAKSEAAPCLELTYNWDPEEYTGGRNFGH
LAYEVDDIYAFCKHLMDGGVTINRPPRDGHMAFVRSPDGISIEILQKGERLPAQEPWASMANTGEW
>Mature_146_residues
MRYLHTMVRVNDLDKALHFYCKLFGLQEIRRYENEKGRFTLVFLAAPGDIDRAKSEAAPCLELTYNWDPEEYTGGRNFGH
LAYEVDDIYAFCKHLMDGGVTINRPPRDGHMAFVRSPDGISIEILQKGERLPAQEPWASMANTGEW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=37.0629370629371, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=34.8484848484849, Blast_Score=89, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=31.6901408450704, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=34.0277777777778, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16784; Mature: 16784

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLHTMVRVNDLDKALHFYCKLFGLQEIRRYENEKGRFTLVFLAAPGDIDRAKSEAAPC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHCCCCE
LELTYNWDPEEYTGGRNFGHLAYEVDDIYAFCKHLMDGGVTINRPPRDGHMAFVRSPDGI
EEEEECCCCHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCC
SIEILQKGERLPAQEPWASMANTGEW
EEEEEECCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MRYLHTMVRVNDLDKALHFYCKLFGLQEIRRYENEKGRFTLVFLAAPGDIDRAKSEAAPC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHCCCCE
LELTYNWDPEEYTGGRNFGHLAYEVDDIYAFCKHLMDGGVTINRPPRDGHMAFVRSPDGI
EEEEECCCCHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCC
SIEILQKGERLPAQEPWASMANTGEW
EEEEEECCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]