Click here to switch to the map view.

The map label for this gene is lexA [H]

Identifier: 150396569

GI number: 150396569

Start: 1435710

End: 1436426

Strand: Direct

Name: lexA [H]

Synonym: Smed_1353

Alternate gene names: 150396569

Gene position: 1435710-1436426 (Clockwise)

Preceding gene: 150396568

Following gene: 150396571

Centisome position: 37.96

GC content: 59.14

Gene sequence:

>717_bases
ATGCTGACCCGCAAGCAACAGGAATTGCTTTTGTTCATTCACGAACGAATGAAGGAGTCCGGGGTGCCTCCTTCCTTCGA
CGAGATGAAGGATGCCCTGGATCTTGCTTCAAAATCCGGGATCCATCGTTTGATCACCGCTTTGGAGGAGCGCGGCTTCA
TTCGCCGGCTACCGAATCGCGCCAGGGCTCTTGAGGTTATCAAGCTGCCGGAGGCCTATGCCGGCAACCCGCAAGTCCGG
CGCGGTTTTTCGCCAAGCGTCATCGAGGGCAGTCTCGGAAAACCAGCCGCACCGGCTCCAGCCCCGAAACCGGCGCCACC
GGCCGAGAACGCATCGGTTGTCGTTCCGGTCATGGGCCGCATTGCCGCGGGCGTGCCCATTTCGGCGATCCAGAACAACA
TGCATGATATTTCGGTGCCGATCGAAATGATCGGCAGTGGCGAACATTACGCGCTGGAAATCAAAGGCGACTCGATGATC
GAAGCCGGTATCCTCGACGGCGATACTGTGATCATTCGCAACGCGAGCACCGCCAGTATCGGAGATATCGTCGTCGCCCT
GATCGACGACGAGGAAGCCACCCTGAAGAGGTTCCGCCGCAAGGGCGCCTCTATCGCGCTCGAGGCCGCCAATCCTGCCT
ATGAAACCCGCATTTTCGGTCCGGACCGGGTCAAGATCCAGGGTAAGCTTGTTGGCCTCATCCGGCGTTACCACTAG

Upstream 100 bases:

>100_bases
ACAGCAGGCAAAATTCACGGAATCTTAGGCACTTGCGGAACACATATGGAACAGATAGGCTCTGTTCTTGATTTGTTTCG
CGATTCTGGAGTGACGCGCC

Downstream 100 bases:

>100_bases
GACCGTTCAAGGTATTCACAATAAAAAGGTTCGCATAGCGAATGAACCCTCTTGCGGATTCCGATCAATCATTCTAAATC
CGCGCCACGATTTGAAAAGA

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNRARALEVIKLPEAYAGNPQVR
RGFSPSVIEGSLGKPAAPAPAPKPAPPAENASVVVPVMGRIAAGVPISAIQNNMHDISVPIEMIGSGEHYALEIKGDSMI
EAGILDGDTVIIRNASTASIGDIVVALIDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH

Sequences:

>Translated_238_residues
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNRARALEVIKLPEAYAGNPQVR
RGFSPSVIEGSLGKPAAPAPAPKPAPPAENASVVVPVMGRIAAGVPISAIQNNMHDISVPIEMIGSGEHYALEIKGDSMI
EAGILDGDTVIIRNASTASIGDIVVALIDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH
>Mature_238_residues
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNRARALEVIKLPEAYAGNPQVR
RGFSPSVIEGSLGKPAAPAPAPKPAPPAENASVVVPVMGRIAAGVPISAIQNNMHDISVPIEMIGSGEHYALEIKGDSMI
EAGILDGDTVIIRNASTASIGDIVVALIDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=236, Percent_Identity=25, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 25781; Mature: 25781

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCH
ARALEVIKLPEAYAGNPQVRRGFSPSVIEGSLGKPAAPAPAPKPAPPAENASVVVPVMGR
HHHEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHH
IAAGVPISAIQNNMHDISVPIEMIGSGEHYALEIKGDSMIEAGILDGDTVIIRNASTASI
HHCCCCHHHHHCCCCEEECCCEEECCCCEEEEEECCCCEEEEEECCCCEEEEECCCCCCC
GDIVVALIDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH
CEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCEEEECCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MLTRKQQELLLFIHERMKESGVPPSFDEMKDALDLASKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCH
ARALEVIKLPEAYAGNPQVRRGFSPSVIEGSLGKPAAPAPAPKPAPPAENASVVVPVMGR
HHHEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHH
IAAGVPISAIQNNMHDISVPIEMIGSGEHYALEIKGDSMIEAGILDGDTVIIRNASTASI
HHCCCCHHHHHCCCCEEECCCEEECCCCEEEEEECCCCEEEEEECCCCEEEEECCCCCCC
GDIVVALIDDEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKIQGKLVGLIRRYH
CEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCEEEECCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]