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The map label for this gene is tig [H]

Identifier: 150396400

GI number: 150396400

Start: 1259019

End: 1260494

Strand: Direct

Name: tig [H]

Synonym: Smed_1181

Alternate gene names: 150396400

Gene position: 1259019-1260494 (Clockwise)

Preceding gene: 150396396

Following gene: 150396404

Centisome position: 33.29

GC content: 59.62

Gene sequence:

>1476_bases
ATGCAGGTTATCGAAACGCTCGCTCAAGGGCTGAAGCGCGAACTCAAGGTCGTCATTCCGGCCGACGAAATGGAAGCTCG
GATGAACGAGCGTCTCGTCGAGGTGAAGGATCGTGTTCGCATCAATGGCTTCCGCCCCGGCAAGGTGCCGGTTGCGCATC
TGAAGAAGGTTTACGGCAAGTCGATCATGGCCGATCTCGTCAACGAGATCGTTCGTGAAAAGCCGACGGAAATCCTCACC
AGCCGCGGCGAGAAGTCTGCGACCCAGCCGGAAATCGGGATGACCGAGGACGAGGCGGAAGCTGACAAGATCCTGAAGGC
CGAAGCGGATTTCGAATTCACCGTCGCCTACGAAATCATTCCGCCGATCGAACTCAAGGACGCGAGCGGCATCAAGGTCA
CTCGCGAAGTCGTTGATGTCGGCGAAGACGAAGTCAACGAGCAGATCGAGCGCATCGCTGAAAGCGCCCGTACTTATGAA
TCGAAGAAGGGCAAGGCCGCCAATGGTGACCGCGTCACGATCGACTACCTGGGCAAGGTCGACGGCGAAGCCTTTGACGG
CGGCAAGGACGAGGATGCGGAACTCGTGCTCGGCTCCAACCGCTTCATCCCCGGCTTCGAAGAGCAGCTCGTTGGCGCGA
AGGCCGGCGATGAAAAGACCATCACGGTCACGTTCCCGGCCGACTACCCGGCAGCCAATCTCGCCGGCAAGGAAGCAACC
TTCGACGTCACGGTGAAGGACGTCGCTGCCGCGGCTCCGATCGAGATCAATGATGAGCTGGCAACGAAGCTCGGCCTCGA
ATCCGTCGACAAGCTGAAGGAAATCGTTCGCGGCCAGATCGAAAGCCAGTTCGGCTCGATAACCCGCCAGAAGGTCAAGC
GCCAGCTTCTAGACCAGCTCGACGAACTCTACCAGTTCGATACGCCCGAGCGCCTCGTCGACGCCGAATTCGAGAACATC
TGGCGCCAGATCAACACCGATCTGCAGCAGGCCGGCAAAACCTTTGCCGATGAAGACACGACGGAAGAGGAAGCCCGCGC
CGAATACCGCAAGCTTGCCCAGCGTCGCGTCCGCCTTGGACTGGTTCTCTCCGAAATAGGCGAGAAGGCCGGTGTCCAGG
TCAGCGATGACGAGATGCAGAATTCACTGTTCCAGCAGCTGCGTCAGTTCCCCGGCCAGGAAAAGGAAATCATCGAATAT
TTCCGCAATACGCCCGGCGCTGCCGCCTCTCTGCGCGCACCGCTCTTCGAGGAGAAGGTCGTCGACCATCTGCTCACCGA
GGTCTCGGTGACCGACAAGAAGGTGTCCAAGGAAGAGCTGACCGCGGAAGACGACGCCGACGAAAAACCGGCCAAGAAGA
CGGCTTCCAAGAAGAAGGCAGCTGCCAAGGCCGATGCCGCCGAGGGCGAAGAAGCCGCAGCGCCGAAAAGGAAGGCTCCG
GCCAAGAAAAAGGCTTCGGACGAGAGCGCCGAGTAA

Upstream 100 bases:

>100_bases
CGGAATTGCGTAAAGCCACTCCCGGCAAATGGATCGGCTCACGTGCTCGCCTAGCCTTATCAAGGGAAGAGCACCGTCAA
CGTGAAGTGAAGGTTTGAAC

Downstream 100 bases:

>100_bases
ATCGAGTGTCTTGCAATGAATTGAAAAAGCCGCCCTCCGGGCGGCTTTTTCTATGCGCTGATGCCGGGCGCCAACGCTGA
CGTTCGCCCAACCCTGACGT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MQVIETLAQGLKRELKVVIPADEMEARMNERLVEVKDRVRINGFRPGKVPVAHLKKVYGKSIMADLVNEIVREKPTEILT
SRGEKSATQPEIGMTEDEAEADKILKAEADFEFTVAYEIIPPIELKDASGIKVTREVVDVGEDEVNEQIERIAESARTYE
SKKGKAANGDRVTIDYLGKVDGEAFDGGKDEDAELVLGSNRFIPGFEEQLVGAKAGDEKTITVTFPADYPAANLAGKEAT
FDVTVKDVAAAAPIEINDELATKLGLESVDKLKEIVRGQIESQFGSITRQKVKRQLLDQLDELYQFDTPERLVDAEFENI
WRQINTDLQQAGKTFADEDTTEEEARAEYRKLAQRRVRLGLVLSEIGEKAGVQVSDDEMQNSLFQQLRQFPGQEKEIIEY
FRNTPGAAASLRAPLFEEKVVDHLLTEVSVTDKKVSKEELTAEDDADEKPAKKTASKKKAAAKADAAEGEEAAAPKRKAP
AKKKASDESAE

Sequences:

>Translated_491_residues
MQVIETLAQGLKRELKVVIPADEMEARMNERLVEVKDRVRINGFRPGKVPVAHLKKVYGKSIMADLVNEIVREKPTEILT
SRGEKSATQPEIGMTEDEAEADKILKAEADFEFTVAYEIIPPIELKDASGIKVTREVVDVGEDEVNEQIERIAESARTYE
SKKGKAANGDRVTIDYLGKVDGEAFDGGKDEDAELVLGSNRFIPGFEEQLVGAKAGDEKTITVTFPADYPAANLAGKEAT
FDVTVKDVAAAAPIEINDELATKLGLESVDKLKEIVRGQIESQFGSITRQKVKRQLLDQLDELYQFDTPERLVDAEFENI
WRQINTDLQQAGKTFADEDTTEEEARAEYRKLAQRRVRLGLVLSEIGEKAGVQVSDDEMQNSLFQQLRQFPGQEKEIIEY
FRNTPGAAASLRAPLFEEKVVDHLLTEVSVTDKKVSKEELTAEDDADEKPAKKTASKKKAAAKADAAEGEEAAAPKRKAP
AKKKASDESAE
>Mature_491_residues
MQVIETLAQGLKRELKVVIPADEMEARMNERLVEVKDRVRINGFRPGKVPVAHLKKVYGKSIMADLVNEIVREKPTEILT
SRGEKSATQPEIGMTEDEAEADKILKAEADFEFTVAYEIIPPIELKDASGIKVTREVVDVGEDEVNEQIERIAESARTYE
SKKGKAANGDRVTIDYLGKVDGEAFDGGKDEDAELVLGSNRFIPGFEEQLVGAKAGDEKTITVTFPADYPAANLAGKEAT
FDVTVKDVAAAAPIEINDELATKLGLESVDKLKEIVRGQIESQFGSITRQKVKRQLLDQLDELYQFDTPERLVDAEFENI
WRQINTDLQQAGKTFADEDTTEEEARAEYRKLAQRRVRLGLVLSEIGEKAGVQVSDDEMQNSLFQQLRQFPGQEKEIIEY
FRNTPGAAASLRAPLFEEKVVDHLLTEVSVTDKKVSKEELTAEDDADEKPAKKTASKKKAAAKADAAEGEEAAAPKRKAP
AKKKASDESAE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=443, Percent_Identity=32.7313769751693, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 54341; Mature: 54341

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVIETLAQGLKRELKVVIPADEMEARMNERLVEVKDRVRINGFRPGKVPVAHLKKVYGK
CHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHH
SIMADLVNEIVREKPTEILTSRGEKSATQPEIGMTEDEAEADKILKAEADFEFTVAYEII
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEC
PPIELKDASGIKVTREVVDVGEDEVNEQIERIAESARTYESKKGKAANGDRVTIDYLGKV
CCCEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
DGEAFDGGKDEDAELVLGSNRFIPGFEEQLVGAKAGDEKTITVTFPADYPAANLAGKEAT
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCE
FDVTVKDVAAAAPIEINDELATKLGLESVDKLKEIVRGQIESQFGSITRQKVKRQLLDQL
EEEEHHHHHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELYQFDTPERLVDAEFENIWRQINTDLQQAGKTFADEDTTEEEARAEYRKLAQRRVRLG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLSEIGEKAGVQVSDDEMQNSLFQQLRQFPGQEKEIIEYFRNTPGAAASLRAPLFEEKV
HHHHHHHHHHCCEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHCCHHHHHH
VDHLLTEVSVTDKKVSKEELTAEDDADEKPAKKTASKKKAAAKADAAEGEEAAAPKRKAP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCC
AKKKASDESAE
CHHCCCCCCCC
>Mature Secondary Structure
MQVIETLAQGLKRELKVVIPADEMEARMNERLVEVKDRVRINGFRPGKVPVAHLKKVYGK
CHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHH
SIMADLVNEIVREKPTEILTSRGEKSATQPEIGMTEDEAEADKILKAEADFEFTVAYEII
HHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEC
PPIELKDASGIKVTREVVDVGEDEVNEQIERIAESARTYESKKGKAANGDRVTIDYLGKV
CCCEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
DGEAFDGGKDEDAELVLGSNRFIPGFEEQLVGAKAGDEKTITVTFPADYPAANLAGKEAT
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCE
FDVTVKDVAAAAPIEINDELATKLGLESVDKLKEIVRGQIESQFGSITRQKVKRQLLDQL
EEEEHHHHHHCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELYQFDTPERLVDAEFENIWRQINTDLQQAGKTFADEDTTEEEARAEYRKLAQRRVRLG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLSEIGEKAGVQVSDDEMQNSLFQQLRQFPGQEKEIIEYFRNTPGAAASLRAPLFEEKV
HHHHHHHHHHCCEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHCCHHHHHH
VDHLLTEVSVTDKKVSKEELTAEDDADEKPAKKTASKKKAAAKADAAEGEEAAAPKRKAP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCC
AKKKASDESAE
CHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]