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The map label for this gene is argS [H]

Identifier: 150396377

GI number: 150396377

Start: 1232913

End: 1234670

Strand: Direct

Name: argS [H]

Synonym: Smed_1158

Alternate gene names: 150396377

Gene position: 1232913-1234670 (Clockwise)

Preceding gene: 150396376

Following gene: 150396378

Centisome position: 32.6

GC content: 58.59

Gene sequence:

>1758_bases
ATGAACCTTTTCACCGACTTCGAAGCGAGAATTAATAGAATTCTGGAATCGATTGATGTTATTCGTGAAAAGCGATCCGA
GTTGGACTTTCGGCGCATCAATGTGGAGCCGCCGCGCGATGCGAGTCACGGTGATGTGGCGACCAATGCGGCAATGGTGC
TTGCCAAGCCGCTCGGCATGAACCCACGCGCGCTGGCGGACCTTATTGGCGACAAGCTGGCACAGGATCCGGAAGTCGCC
GAGGTTTCCGTTGCCGGTCCCGGGTTCATCAATGTCCGCCTGTCGGTTTCCTATTGGCAGAAGCTTCTGGCGGTCATAAC
GCGCGCCGGCGTCGATTACGGTCGCAGCGCATTAGGCGCCGGCCGCAAGATCAACGTGGAATATGTCTCGGCCAATCCGA
CCGGCCCTATGCATGTCGGCCACTGCCGCGGTGCGGTCGTCGGAGACGCGCTTGCCAATCTTTTGGCCTTTTCGGGTTTC
GACGTAACCAAGGAATACTACATCAACGATGCGGGTTCGCAGATCGAAGTGCTCGCCCGGTCGGCATTCCTGCGTTATCG
TCAGGCGCTTGGCGAGGACATAGGCGAGATTCCGGCGGGCCTCTACCCCGGCGACTATCTCGTACCGGCGGGAGAGGCGC
TCGCGGACGAGTACGGCACGAGCCTCAGGATCATGCCGGAGGACAAATGGATGCCGCTCGTCAAGGAGCGCGTCATCGAC
GCAATGATGGCGATGATCCGGGAGGATCTTGCGGCACTCAATGTCAATCACGACGTGTTCTTTTCCGAGCGGGCCTTGCA
CGACAACGGTGCAGCGCGAATCCGCACGGCGATCAACGATCTGACTTTTAAGGGGCATGTCTACAAAGGCATGCTGCCGC
CGCCGAAAGGCCAGTTGCCGGAAGATTGGGAAGACAGGGAGCAGACGCTCTTCCGCTCGACCGAGGTCGGTGACGACATC
GATCGCCCGCTTATCAAATCCGACGGCAGCTATACCTACTTCGCAGCCGACGTCGCCTACTTCAAGGACAAGTTCGACCG
CGGTTTCGACGAAATGATCTACGTGCTCGGCGCCGACCACGGCGGCTATGTAAAGCGGCTGGAGGCGCTTGCGCGTGCGA
TCTCTGGCGGATCGGCAAAGTTGACCGTGCTGCTGTGCCAGCTCGTGAAGCTCTACCGCAATGGCGAGCCGGTGAAGATG
TCCAAGCGCTCGGGCGATTTCGTGACGCTGCGCGAAGTCGTGGACGAAGTCGGGCGCGATCCCGTCCGGTTCATGATGCT
GTACCGCAAGAGCTCCGAGCCTCTCGATTTCGACTTTGCCAAGGTGACGGAACAGTCGAAGGACAACCCTGTCTTTTACG
TGCAATACGCCCATGCGCGCTGCCGTTCCGTTTTCCGCCAGGCTGCTGAAGCATTTCCTGACCTGGATCTGTCGTCCATC
GATCTCGCCGGCGCGGCGGGCGCTATTGCCGATCCCACGGAAATGCAGCTCGTCGCCAAGCTCGCAGAATATCCCCGTGT
AGTGGAGGCCGCAGCATTCTCGCATGAGCCGCACCGAATCGCTTTTTACCTGTATGATCTTGCGGCGGTCTTCCATGGCC
ACTGGAACAAAGGTAAGGAAAACCCGGCATTACGTTTTGTTAACGATAAGAATAGAGAATTAAGCATTGCCAGACTCGGG
CTGGTGCATGCTGTCGCCTCGGTATTGAAGTCGGGCCTGTCGATTACAGGGACTTCGGCACCGGATGAGATGCGGTAA

Upstream 100 bases:

>100_bases
AGTGACGCCCCCGCGTGGAGGCCGCGGGTTTTCGATTGACCCGGCCTCCTTTTGAATTTGCGCCATTCTCCAGTGTGGCT
GGAAAAAGCGGACGGATTAA

Downstream 100 bases:

>100_bases
GTAGTGTCACCAATTCCCCACAATGCACTGGCAATTCAACGCAGGCAGTTGTGAGTGGAGAGTATGGCAGACAAACAATT
CGCACGAAGCGGACCGGCGG

Product: arginyl-tRNA synthetase

Products: NA

Alternate protein names: Arginine--tRNA ligase; ArgRS [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MNLFTDFEARINRILESIDVIREKRSELDFRRINVEPPRDASHGDVATNAAMVLAKPLGMNPRALADLIGDKLAQDPEVA
EVSVAGPGFINVRLSVSYWQKLLAVITRAGVDYGRSALGAGRKINVEYVSANPTGPMHVGHCRGAVVGDALANLLAFSGF
DVTKEYYINDAGSQIEVLARSAFLRYRQALGEDIGEIPAGLYPGDYLVPAGEALADEYGTSLRIMPEDKWMPLVKERVID
AMMAMIREDLAALNVNHDVFFSERALHDNGAARIRTAINDLTFKGHVYKGMLPPPKGQLPEDWEDREQTLFRSTEVGDDI
DRPLIKSDGSYTYFAADVAYFKDKFDRGFDEMIYVLGADHGGYVKRLEALARAISGGSAKLTVLLCQLVKLYRNGEPVKM
SKRSGDFVTLREVVDEVGRDPVRFMMLYRKSSEPLDFDFAKVTEQSKDNPVFYVQYAHARCRSVFRQAAEAFPDLDLSSI
DLAGAAGAIADPTEMQLVAKLAEYPRVVEAAAFSHEPHRIAFYLYDLAAVFHGHWNKGKENPALRFVNDKNRELSIARLG
LVHAVASVLKSGLSITGTSAPDEMR

Sequences:

>Translated_585_residues
MNLFTDFEARINRILESIDVIREKRSELDFRRINVEPPRDASHGDVATNAAMVLAKPLGMNPRALADLIGDKLAQDPEVA
EVSVAGPGFINVRLSVSYWQKLLAVITRAGVDYGRSALGAGRKINVEYVSANPTGPMHVGHCRGAVVGDALANLLAFSGF
DVTKEYYINDAGSQIEVLARSAFLRYRQALGEDIGEIPAGLYPGDYLVPAGEALADEYGTSLRIMPEDKWMPLVKERVID
AMMAMIREDLAALNVNHDVFFSERALHDNGAARIRTAINDLTFKGHVYKGMLPPPKGQLPEDWEDREQTLFRSTEVGDDI
DRPLIKSDGSYTYFAADVAYFKDKFDRGFDEMIYVLGADHGGYVKRLEALARAISGGSAKLTVLLCQLVKLYRNGEPVKM
SKRSGDFVTLREVVDEVGRDPVRFMMLYRKSSEPLDFDFAKVTEQSKDNPVFYVQYAHARCRSVFRQAAEAFPDLDLSSI
DLAGAAGAIADPTEMQLVAKLAEYPRVVEAAAFSHEPHRIAFYLYDLAAVFHGHWNKGKENPALRFVNDKNRELSIARLG
LVHAVASVLKSGLSITGTSAPDEMR
>Mature_585_residues
MNLFTDFEARINRILESIDVIREKRSELDFRRINVEPPRDASHGDVATNAAMVLAKPLGMNPRALADLIGDKLAQDPEVA
EVSVAGPGFINVRLSVSYWQKLLAVITRAGVDYGRSALGAGRKINVEYVSANPTGPMHVGHCRGAVVGDALANLLAFSGF
DVTKEYYINDAGSQIEVLARSAFLRYRQALGEDIGEIPAGLYPGDYLVPAGEALADEYGTSLRIMPEDKWMPLVKERVID
AMMAMIREDLAALNVNHDVFFSERALHDNGAARIRTAINDLTFKGHVYKGMLPPPKGQLPEDWEDREQTLFRSTEVGDDI
DRPLIKSDGSYTYFAADVAYFKDKFDRGFDEMIYVLGADHGGYVKRLEALARAISGGSAKLTVLLCQLVKLYRNGEPVKM
SKRSGDFVTLREVVDEVGRDPVRFMMLYRKSSEPLDFDFAKVTEQSKDNPVFYVQYAHARCRSVFRQAAEAFPDLDLSSI
DLAGAAGAIADPTEMQLVAKLAEYPRVVEAAAFSHEPHRIAFYLYDLAAVFHGHWNKGKENPALRFVNDKNRELSIARLG
LVHAVASVLKSGLSITGTSAPDEMR

Specific function: Unknown

COG id: COG0018

COG function: function code J; Arginyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15149476, Length=149, Percent_Identity=34.2281879194631, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1788184, Length=590, Percent_Identity=26.271186440678, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71985061, Length=610, Percent_Identity=24.4262295081967, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71985068, Length=560, Percent_Identity=25, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320548, Length=566, Percent_Identity=24.2049469964664, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859963, Length=587, Percent_Identity=23.5093696763203, Blast_Score=96, Evalue=8e-20,

Paralogues:

None

Copy number: 789 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR001278
- InterPro:   IPR015945
- InterPro:   IPR005148
- InterPro:   IPR008909
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF03485 Arg_tRNA_synt_N; PF05746 DALR_1; PF00750 tRNA-synt_1d [H]

EC number: =6.1.1.19 [H]

Molecular weight: Translated: 64744; Mature: 64744

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFTDFEARINRILESIDVIREKRSELDFRRINVEPPRDASHGDVATNAAMVLAKPLGM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHEEECCCCC
NPRALADLIGDKLAQDPEVAEVSVAGPGFINVRLSVSYWQKLLAVITRAGVDYGRSALGA
CHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHHCHHHCHHHHCC
GRKINVEYVSANPTGPMHVGHCRGAVVGDALANLLAFSGFDVTKEYYINDAGSQIEVLAR
CCEEEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCHHHEECCCCCHHHHHHH
SAFLRYRQALGEDIGEIPAGLYPGDYLVPAGEALADEYGTSLRIMPEDKWMPLVKERVID
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHH
AMMAMIREDLAALNVNHDVFFSERALHDNGAARIRTAINDLTFKGHVYKGMLPPPKGQLP
HHHHHHHHHHHHCCCCCCEEECCHHHCCCCHHHHHHHHHHCEECCCEEECCCCCCCCCCC
EDWEDREQTLFRSTEVGDDIDRPLIKSDGSYTYFAADVAYFKDKFDRGFDEMIYVLGADH
CCHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEEHHHHHHHHHHCCCHHHEEEEEECCC
GGYVKRLEALARAISGGSAKLTVLLCQLVKLYRNGEPVKMSKRSGDFVTLREVVDEVGRD
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEHHHHHHHHCCC
PVRFMMLYRKSSEPLDFDFAKVTEQSKDNPVFYVQYAHARCRSVFRQAAEAFPDLDLSSI
HHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCC
DLAGAAGAIADPTEMQLVAKLAEYPRVVEAAAFSHEPHRIAFYLYDLAAVFHGHWNKGKE
CCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCC
NPALRFVNDKNRELSIARLGLVHAVASVLKSGLSITGTSAPDEMR
CCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
>Mature Secondary Structure
MNLFTDFEARINRILESIDVIREKRSELDFRRINVEPPRDASHGDVATNAAMVLAKPLGM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHEEECCCCC
NPRALADLIGDKLAQDPEVAEVSVAGPGFINVRLSVSYWQKLLAVITRAGVDYGRSALGA
CHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHHCHHHCHHHHCC
GRKINVEYVSANPTGPMHVGHCRGAVVGDALANLLAFSGFDVTKEYYINDAGSQIEVLAR
CCEEEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCHHHEECCCCCHHHHHHH
SAFLRYRQALGEDIGEIPAGLYPGDYLVPAGEALADEYGTSLRIMPEDKWMPLVKERVID
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHH
AMMAMIREDLAALNVNHDVFFSERALHDNGAARIRTAINDLTFKGHVYKGMLPPPKGQLP
HHHHHHHHHHHHCCCCCCEEECCHHHCCCCHHHHHHHHHHCEECCCEEECCCCCCCCCCC
EDWEDREQTLFRSTEVGDDIDRPLIKSDGSYTYFAADVAYFKDKFDRGFDEMIYVLGADH
CCHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEEEHHHHHHHHHHCCCHHHEEEEEECCC
GGYVKRLEALARAISGGSAKLTVLLCQLVKLYRNGEPVKMSKRSGDFVTLREVVDEVGRD
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEHHHHHHHHCCC
PVRFMMLYRKSSEPLDFDFAKVTEQSKDNPVFYVQYAHARCRSVFRQAAEAFPDLDLSSI
HHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCC
DLAGAAGAIADPTEMQLVAKLAEYPRVVEAAAFSHEPHRIAFYLYDLAAVFHGHWNKGKE
CCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCCC
NPALRFVNDKNRELSIARLGLVHAVASVLKSGLSITGTSAPDEMR
CCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]