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The map label for this gene is tsf [H]

Identifier: 150396351

GI number: 150396351

Start: 1200665

End: 1201588

Strand: Direct

Name: tsf [H]

Synonym: Smed_1132

Alternate gene names: 150396351

Gene position: 1200665-1201588 (Clockwise)

Preceding gene: 150396350

Following gene: 150396352

Centisome position: 31.75

GC content: 61.9

Gene sequence:

>924_bases
ATGACTGTTACCGCCGCAATGGTGAAGGAGCTGCGCGAAAAGACCGGCGCAGGCATGATGGACTGCAAGAAGGCGCTTGC
CGAAACCAATGGCGACATGGAAGCCGCCATCGACTGGCTGCGCGCCAAGGGCATCGCCAAGGCTGACAAGAAGTCCGGCC
GCACTGCCGCAGAAGGCCTCATCGGCATTGCCAGCGCAGGCGCCAAGGCCGTCGTCGTCGAAATCAACTCCGAAACCGAC
TTCGTTGCCCGCAACGATGCCTTCCAGGAGCTCGTCCGCGGCGTCGCCAATGTTGCGCTCGGTACGGACGGCAGCGTCGC
AGCGGTCTCAAAGGCGACCTATCCGGCCACCGGCAAGTCCGTCGAAGATACGATCAAGGATGCTATCGCCACCATCGGCG
AGAACATGACGCTGCGCCGCTCGGCAATGCTCGAGGTCGAGGACGGCGTCGTCGCGACCTATGTGCACAATGCCGCAGGC
GAGGGTATCGGCAAGCTCGGCGTGCTCGTTGCGTTGAAGTCCTCCGGCGACAAGGAAGCCCTGAACGCGATCGGCCGCCA
GGTTGCAATGCACGTGGCCGCGACCAACCCGCTCGCCGTCCGTTCGAGCGAAATCGACCCTGCGGTCGCCGAACGCGAGC
GCAACGTGTTCATCGAGCAGTCGCGCGCTTCCGGCAAGCCGGACAATATCATCGAGAAGATGGTCGATGGCCGCATGCGC
AAGTTCTTCGAGGAAGTTGCCCTTCTCTCGCAGGCTTTCGTCATGAATCCTGATCAGACGGTGGAAGCGGCGATCAAGGA
AGCGGAAAAATCCGTTGGTGCGCCCATCGAAGTCGCTGGCATTGCCCGTTTGCTGCTCGGCGAAGGTGTCGAAAAGGAAG
AAAGCGATTTCGCGGCTGAAGTGGCAGCTGCCGCGAAGGGTTGA

Upstream 100 bases:

>100_bases
GCTTCATAACGATGTCACACATAAGGGCCATTTCCTGCCAAACCTTCTGGCACTGCGCCAATCCCGGTCGGCGCGCCAGC
AACAGATAAAGAGGCACAAG

Downstream 100 bases:

>100_bases
TTTCTTCACATCGAACAGGAAAACGTGGGGGCATCGCGTGACAACGCGGTGCCCTTCGTGTATCCGGCTCCAGTCGATGC
GTAGGACCCGCCGGAGCGGT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTVTAAMVKELREKTGAGMMDCKKALAETNGDMEAAIDWLRAKGIAKADKKSGRTAAEGLIGIASAGAKAVVVEINSETD
FVARNDAFQELVRGVANVALGTDGSVAAVSKATYPATGKSVEDTIKDAIATIGENMTLRRSAMLEVEDGVVATYVHNAAG
EGIGKLGVLVALKSSGDKEALNAIGRQVAMHVAATNPLAVRSSEIDPAVAERERNVFIEQSRASGKPDNIIEKMVDGRMR
KFFEEVALLSQAFVMNPDQTVEAAIKEAEKSVGAPIEVAGIARLLLGEGVEKEESDFAAEVAAAAKG

Sequences:

>Translated_307_residues
MTVTAAMVKELREKTGAGMMDCKKALAETNGDMEAAIDWLRAKGIAKADKKSGRTAAEGLIGIASAGAKAVVVEINSETD
FVARNDAFQELVRGVANVALGTDGSVAAVSKATYPATGKSVEDTIKDAIATIGENMTLRRSAMLEVEDGVVATYVHNAAG
EGIGKLGVLVALKSSGDKEALNAIGRQVAMHVAATNPLAVRSSEIDPAVAERERNVFIEQSRASGKPDNIIEKMVDGRMR
KFFEEVALLSQAFVMNPDQTVEAAIKEAEKSVGAPIEVAGIARLLLGEGVEKEESDFAAEVAAAAKG
>Mature_306_residues
TVTAAMVKELREKTGAGMMDCKKALAETNGDMEAAIDWLRAKGIAKADKKSGRTAAEGLIGIASAGAKAVVVEINSETDF
VARNDAFQELVRGVANVALGTDGSVAAVSKATYPATGKSVEDTIKDAIATIGENMTLRRSAMLEVEDGVVATYVHNAAGE
GIGKLGVLVALKSSGDKEALNAIGRQVAMHVAATNPLAVRSSEIDPAVAERERNVFIEQSRASGKPDNIIEKMVDGRMRK
FFEEVALLSQAFVMNPDQTVEAAIKEAEKSVGAPIEVAGIARLLLGEGVEKEESDFAAEVAAAAKG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=231, Percent_Identity=37.6623376623377, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI291084500, Length=251, Percent_Identity=34.2629482071713, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI291084502, Length=100, Percent_Identity=47, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI291084498, Length=100, Percent_Identity=47, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1786366, Length=304, Percent_Identity=43.421052631579, Blast_Score=215, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17561440, Length=277, Percent_Identity=29.2418772563177, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921466, Length=307, Percent_Identity=29.6416938110749, Blast_Score=104, Evalue=6e-23,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32060; Mature: 31929

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTAAMVKELREKTGAGMMDCKKALAETNGDMEAAIDWLRAKGIAKADKKSGRTAAEGL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHH
IGIASAGAKAVVVEINSETDFVARNDAFQELVRGVANVALGTDGSVAAVSKATYPATGKS
HHHCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHCCCCCCEEEHCCCCCCCCCCH
VEDTIKDAIATIGENMTLRRSAMLEVEDGVVATYVHNAAGEGIGKLGVLVALKSSGDKEA
HHHHHHHHHHHHCCCCEEHHHHHEEECCCEEEEHHHHHHCCCHHHHEEEEEECCCCCHHH
LNAIGRQVAMHVAATNPLAVRSSEIDPAVAERERNVFIEQSRASGKPDNIIEKMVDGRMR
HHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHH
KFFEEVALLSQAFVMNPDQTVEAAIKEAEKSVGAPIEVAGIARLLLGEGVEKEESDFAAE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
VAAAAKG
HHHHCCC
>Mature Secondary Structure 
TVTAAMVKELREKTGAGMMDCKKALAETNGDMEAAIDWLRAKGIAKADKKSGRTAAEGL
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHH
IGIASAGAKAVVVEINSETDFVARNDAFQELVRGVANVALGTDGSVAAVSKATYPATGKS
HHHCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHCCCCCCEEEHCCCCCCCCCCH
VEDTIKDAIATIGENMTLRRSAMLEVEDGVVATYVHNAAGEGIGKLGVLVALKSSGDKEA
HHHHHHHHHHHHCCCCEEHHHHHEEECCCEEEEHHHHHHCCCHHHHEEEEEECCCCCHHH
LNAIGRQVAMHVAATNPLAVRSSEIDPAVAERERNVFIEQSRASGKPDNIIEKMVDGRMR
HHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHHHH
KFFEEVALLSQAFVMNPDQTVEAAIKEAEKSVGAPIEVAGIARLLLGEGVEKEESDFAAE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
VAAAAKG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]