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The map label for this gene is mlc [H]

Identifier: 150396345

GI number: 150396345

Start: 1194060

End: 1195175

Strand: Direct

Name: mlc [H]

Synonym: Smed_1126

Alternate gene names: 150396345

Gene position: 1194060-1195175 (Clockwise)

Preceding gene: 150396343

Following gene: 150396346

Centisome position: 31.57

GC content: 63.35

Gene sequence:

>1116_bases
GTGGGTCTGCATCACGGCCAGGTTCTGCGCATTCTGCGCGACGAGGGCCCCAACTCGAGAGCTATCCTCGCGCGCCGGAG
CAAATTGTCCGCGACGACTCTGACTCATGTCACGGCGCAGCTTCTCGCGGACGGTTCTGTTGTCGAGGAGGAACCTGGGG
CTAGTGGTGGGCTCGGCCGACCCGCCTTGGCGATCCGACTCTTGCCCGATGCTCATCATGTCGCCGGCGTGCATATCGGC
GCCGGCCTCGTGCAGTTAACGGTCACAAATCTCCTGGGCTCGCCAAAGGCCGCAGCTTCATTCGAGTACGGCCTTCCGAA
CACATCGCCAGAAGCCTTGACCACCCGGATCGCCGACGAAATTGAAAGGCTGAAGGCCGTCAGCGGCGTCGAAGATCTGC
TCGGCGTCGGAGTGGGCGTGCCGGGGCCGGTCGATGCCGGCCAACGATCCATCCTGGCGTCGATCAATACCGGTTGGCGC
AATCTGCCGCTGGCCGGCATGCTGGAACGGCGAACGGGCCTTTCCGTGGTGCTTGAGCATAACGTAAGTGCAATGGCAGT
TGCCGAAGCGCGCTTTGGGCTCGGGCGCAATGTTGCGGCGGTGCTGTTCGTCTATCTGCGCTCTGGTCTCGGGGCAGGGC
TGGTCGTCGATGGAATGCCGTTCCGACCAGGCGGACATGGTGCGGTCGAACTCGGTCACATCCAGATATCGCAAGACGGG
CCGCTGTGCAGTTGCGGCAACCATGGCTGCCTTGAGAGCTATGTGAACGAGCGCGTTCTGATGCGCTCTCTTGGGTCGGA
GGGGTCCGCACCGGCCGATCTGCTGATGCAGTTGGAACGCACGGCATCCTGGAAACCGACACTGGCGCGCCTGACTGACT
CACTCGCCATCGCGATCAGTCTCCTGACGCCCGATCTGATCGTCTTCGGCGGCCATCTCGGCGACGCGCCGGATAGCCTT
TTCTCGCACCTGCGGGAACACCTGCCGCCACGCGTCATGCCGCACATGCGCGAGATACTGCGCCTTGAGCGGACCAGCTT
TGGGTCGCAGTCGGGCCCGATCGGCGGTGCTGCGGTCGCCTTGGATCACTTTTTCTATTCAGGAGCCCGCCTTTGA

Upstream 100 bases:

>100_bases
GGAGCCGACGCATGAACCCTCTGCGATCGCAAATTGACATCTTCCGATTTATTTTCTAAAGTTTAGAAAATAAATATGAG
ACGAAGGGGAACGACTACAG

Downstream 100 bases:

>100_bases
GTACCCTGACGACAAAAGACGAGGATATCGCTGCGAAGCGGCTTTCATCGGCAAACCTGGCTCCCATTGCCTTGAACTTG
ATCGCTTTCACGTTGGCCAT

Product: ROK family protein

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MGLHHGQVLRILRDEGPNSRAILARRSKLSATTLTHVTAQLLADGSVVEEEPGASGGLGRPALAIRLLPDAHHVAGVHIG
AGLVQLTVTNLLGSPKAAASFEYGLPNTSPEALTTRIADEIERLKAVSGVEDLLGVGVGVPGPVDAGQRSILASINTGWR
NLPLAGMLERRTGLSVVLEHNVSAMAVAEARFGLGRNVAAVLFVYLRSGLGAGLVVDGMPFRPGGHGAVELGHIQISQDG
PLCSCGNHGCLESYVNERVLMRSLGSEGSAPADLLMQLERTASWKPTLARLTDSLAIAISLLTPDLIVFGGHLGDAPDSL
FSHLREHLPPRVMPHMREILRLERTSFGSQSGPIGGAAVALDHFFYSGARL

Sequences:

>Translated_371_residues
MGLHHGQVLRILRDEGPNSRAILARRSKLSATTLTHVTAQLLADGSVVEEEPGASGGLGRPALAIRLLPDAHHVAGVHIG
AGLVQLTVTNLLGSPKAAASFEYGLPNTSPEALTTRIADEIERLKAVSGVEDLLGVGVGVPGPVDAGQRSILASINTGWR
NLPLAGMLERRTGLSVVLEHNVSAMAVAEARFGLGRNVAAVLFVYLRSGLGAGLVVDGMPFRPGGHGAVELGHIQISQDG
PLCSCGNHGCLESYVNERVLMRSLGSEGSAPADLLMQLERTASWKPTLARLTDSLAIAISLLTPDLIVFGGHLGDAPDSL
FSHLREHLPPRVMPHMREILRLERTSFGSQSGPIGGAAVALDHFFYSGARL
>Mature_370_residues
GLHHGQVLRILRDEGPNSRAILARRSKLSATTLTHVTAQLLADGSVVEEEPGASGGLGRPALAIRLLPDAHHVAGVHIGA
GLVQLTVTNLLGSPKAAASFEYGLPNTSPEALTTRIADEIERLKAVSGVEDLLGVGVGVPGPVDAGQRSILASINTGWRN
LPLAGMLERRTGLSVVLEHNVSAMAVAEARFGLGRNVAAVLFVYLRSGLGAGLVVDGMPFRPGGHGAVELGHIQISQDGP
LCSCGNHGCLESYVNERVLMRSLGSEGSAPADLLMQLERTASWKPTLARLTDSLAIAISLLTPDLIVFGGHLGDAPDSLF
SHLREHLPPRVMPHMREILRLERTSFGSQSGPIGGAAVALDHFFYSGARL

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI1787878, Length=387, Percent_Identity=28.6821705426357, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786891, Length=380, Percent_Identity=28.9473684210526, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787363, Length=244, Percent_Identity=26.6393442622951, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790522, Length=244, Percent_Identity=28.2786885245902, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082127, Length=312, Percent_Identity=21.1538461538462, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081733, Length=206, Percent_Identity=26.2135922330097, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 38964; Mature: 38833

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLHHGQVLRILRDEGPNSRAILARRSKLSATTLTHVTAQLLADGSVVEEEPGASGGLGR
CCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PALAIRLLPDAHHVAGVHIGAGLVQLTVTNLLGSPKAAASFEYGLPNTSPEALTTRIADE
CEEEEEECCCCHHHHEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHH
IERLKAVSGVEDLLGVGVGVPGPVDAGQRSILASINTGWRNLPLAGMLERRTGLSVVLEH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECCCCCCCCCHHHHHHHCCCCEEEEEC
NVSAMAVAEARFGLGRNVAAVLFVYLRSGLGAGLVVDGMPFRPGGHGAVELGHIQISQDG
CCCCEEHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEEECCCC
PLCSCGNHGCLESYVNERVLMRSLGSEGSAPADLLMQLERTASWKPTLARLTDSLAIAIS
CEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LLTPDLIVFGGHLGDAPDSLFSHLREHLPPRVMPHMREILRLERTSFGSQSGPIGGAAVA
HHCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LDHFFYSGARL
HHHHHHCCCCC
>Mature Secondary Structure 
GLHHGQVLRILRDEGPNSRAILARRSKLSATTLTHVTAQLLADGSVVEEEPGASGGLGR
CCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PALAIRLLPDAHHVAGVHIGAGLVQLTVTNLLGSPKAAASFEYGLPNTSPEALTTRIADE
CEEEEEECCCCHHHHEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHH
IERLKAVSGVEDLLGVGVGVPGPVDAGQRSILASINTGWRNLPLAGMLERRTGLSVVLEH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEECCCCCCCCCHHHHHHHCCCCEEEEEC
NVSAMAVAEARFGLGRNVAAVLFVYLRSGLGAGLVVDGMPFRPGGHGAVELGHIQISQDG
CCCCEEHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEEECCCC
PLCSCGNHGCLESYVNERVLMRSLGSEGSAPADLLMQLERTASWKPTLARLTDSLAIAIS
CEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LLTPDLIVFGGHLGDAPDSLFSHLREHLPPRVMPHMREILRLERTSFGSQSGPIGGAAVA
HHCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LDHFFYSGARL
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]