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The map label for this gene is ytxM [H]

Identifier: 150396331

GI number: 150396331

Start: 1182501

End: 1183286

Strand: Direct

Name: ytxM [H]

Synonym: Smed_1112

Alternate gene names: 150396331

Gene position: 1182501-1183286 (Clockwise)

Preceding gene: 150396328

Following gene: 150396332

Centisome position: 31.27

GC content: 62.47

Gene sequence:

>786_bases
ATGGACTTGAATCCTCCGCTATTTTCCCGCTTCGTGCATGACGGACTGGAAATCGCCTATTTCGACGAGGGCGACCCGTC
GGGCGATCCGATTCTGCTCATTCACGGCTTTGCTTCGAGCGCCAATGTGAACTGGGTATTTCCGGGCTGGCTCAAGACGC
TTGGGGACGCCGGATACCGGGTCATCGCGCTGGACAATCGCGGACACGGGCAAAGCAGCAAGCCGCATGACCCCTCGCTC
TATCACCCGCATCAGATGGCCGGGGATGCGGCAGCGCTTCTGGTGCATCTCGGGATCGGCGAAGCGCATGTCATGGGATA
TTCGATGGGCGCGCGGATCTCTGCCTTCCTGGCCCTGCAACACCCGGCCAGGGTTCGTTCGCTGGTGTTCGGTGGTCTCG
GAATAGGCATGATCACCGGCGTGGGAGAGTGGGATCCAATTGCCGACGCGCTGCTTGCACCTTCGTTGGAGGATGTGACG
CACGAGCGCGGCCGGACGTTTCGCGCCTTTGCAGACCAGACGAAAAGCGATCGTCAGGCGCTCGCCGCCTGCATATCGAC
GTCGCGTGATCTGCTTAGCGCCGAAGAGGTCGGCCGCATAGACGTGCCGGTGCTGATCGGCGTCGGGACAAAGGACGACA
TCGCCGGATCGGCCCAGGAGCTTGCCGCGCTGATGCAGCATGCCCTGGCACTCGACATTCCCGGGCGCGATCACATGCTT
GCGGTGGGTGACCGCGTGTTCAAGAAGGCCGTGCTGGAATTCCTTGCCGAGGTCGGGCGCGCCTAA

Upstream 100 bases:

>100_bases
ATGTGTAGCAGCAACATGCTGCCCCCTGTGGTTTTTGGGTTTTCTGCAGTTGCCTCAGCAACTAACCTCCTTGCCAATGA
ACCAATACCAGGAAGACGAG

Downstream 100 bases:

>100_bases
ATATCGATAATTTCAGGACGCAATTTACCCGACTTGGATCAACTCCGAGCCACTACCGGGAGGTGAAATCGGGTCAGCCC
ATTTATGTCGGCCCGGAATT

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MDLNPPLFSRFVHDGLEIAYFDEGDPSGDPILLIHGFASSANVNWVFPGWLKTLGDAGYRVIALDNRGHGQSSKPHDPSL
YHPHQMAGDAAALLVHLGIGEAHVMGYSMGARISAFLALQHPARVRSLVFGGLGIGMITGVGEWDPIADALLAPSLEDVT
HERGRTFRAFADQTKSDRQALAACISTSRDLLSAEEVGRIDVPVLIGVGTKDDIAGSAQELAALMQHALALDIPGRDHML
AVGDRVFKKAVLEFLAEVGRA

Sequences:

>Translated_261_residues
MDLNPPLFSRFVHDGLEIAYFDEGDPSGDPILLIHGFASSANVNWVFPGWLKTLGDAGYRVIALDNRGHGQSSKPHDPSL
YHPHQMAGDAAALLVHLGIGEAHVMGYSMGARISAFLALQHPARVRSLVFGGLGIGMITGVGEWDPIADALLAPSLEDVT
HERGRTFRAFADQTKSDRQALAACISTSRDLLSAEEVGRIDVPVLIGVGTKDDIAGSAQELAALMQHALALDIPGRDHML
AVGDRVFKKAVLEFLAEVGRA
>Mature_261_residues
MDLNPPLFSRFVHDGLEIAYFDEGDPSGDPILLIHGFASSANVNWVFPGWLKTLGDAGYRVIALDNRGHGQSSKPHDPSL
YHPHQMAGDAAALLVHLGIGEAHVMGYSMGARISAFLALQHPARVRSLVFGGLGIGMITGVGEWDPIADALLAPSLEDVT
HERGRTFRAFADQTKSDRQALAACISTSRDLLSAEEVGRIDVPVLIGVGTKDDIAGSAQELAALMQHALALDIPGRDHML
AVGDRVFKKAVLEFLAEVGRA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 27888; Mature: 27888

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNPPLFSRFVHDGLEIAYFDEGDPSGDPILLIHGFASSANVNWVFPGWLKTLGDAGYR
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEECHHHHHHCCCCEE
VIALDNRGHGQSSKPHDPSLYHPHQMAGDAAALLVHLGIGEAHVMGYSMGARISAFLALQ
EEEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCHHHEECCCCHHHHHHHHHC
HPARVRSLVFGGLGIGMITGVGEWDPIADALLAPSLEDVTHERGRTFRAFADQTKSDRQA
CHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHHCCCEEHHHHHCCHHHHHH
LAACISTSRDLLSAEEVGRIDVPVLIGVGTKDDIAGSAQELAALMQHALALDIPGRDHML
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHEECCCCCCCEE
AVGDRVFKKAVLEFLAEVGRA
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLNPPLFSRFVHDGLEIAYFDEGDPSGDPILLIHGFASSANVNWVFPGWLKTLGDAGYR
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEECHHHHHHCCCCEE
VIALDNRGHGQSSKPHDPSLYHPHQMAGDAAALLVHLGIGEAHVMGYSMGARISAFLALQ
EEEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCCHHHEECCCCHHHHHHHHHC
HPARVRSLVFGGLGIGMITGVGEWDPIADALLAPSLEDVTHERGRTFRAFADQTKSDRQA
CHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHHCCCEEHHHHHCCHHHHHH
LAACISTSRDLLSAEEVGRIDVPVLIGVGTKDDIAGSAQELAALMQHALALDIPGRDHML
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHEECCCCCCCEE
AVGDRVFKKAVLEFLAEVGRA
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8566759; 9387221; 9384377 [H]