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The map label for this gene is cysG [H]

Identifier: 150396318

GI number: 150396318

Start: 1168901

End: 1170355

Strand: Direct

Name: cysG [H]

Synonym: Smed_1099

Alternate gene names: 150396318

Gene position: 1168901-1170355 (Clockwise)

Preceding gene: 150396315

Following gene: 150396319

Centisome position: 30.91

GC content: 65.7

Gene sequence:

>1455_bases
ATGCCGCAGAAACTATCCGTCTTTCCGGCCTTCTTTCGCGTCGCGCAGAAAAGCGTGGCGGTGTTCGGCAATGGGGACGA
GGCCTTCGCCAAGGTGCGGCTGCTGCTGAGTACCGAGGCGCGCATCGTCGCCTATGCGGATCAGCCCGAGGCGGCTTTCG
AAGCGTTCCTGAATGCCAACGGTATCGATACCATCCGCAAGCCTTTTGATGCTCGCCAGGTCGAGGGCGCCGTTCTCGTC
TTCGCGGCAACGGGCGATGCGGCTTTCGACAGGATCATCGTGGCCGCGGCGCGGGAACGGAAAATCCCCGCAAATGCCGT
CGACCAGCCGGACTACTGCGATTTTCTGACGCCCGCACTCGTCAACCGCGCGCCTGTGGCCGTTGCGATCGGCACGGAAG
GGGCGGGGCCGGTTCTGGCGCAGATGATTCGCGCGCAGATCGATCAGTTGCTCTCGCCGTCGCTGGGCATCGTTGCTTCA
TTGGCGGCAAGCTATCGCGACGCCGTCGATCGCCTCGTTCCGCGCGGTGTCGCACGCCGAGTCTTCTGGCGGCGCTTCTT
CTCCGGTCCGGTGGCCGATCACGTCGCACTTGGCGATCTTGCATCCGCACGCCGCGAGGCCTCGAAGCTTCTGGATGCGG
CCGGTGGCGTACGCGGTCATGTCTGGCTCGTCGGCGCCGGTCCCGGTGCGGAAGACCTACTGACGCTCAGAGCCCAGCGC
GTTATGATGGAAGCGGACGCGATCGTCTATGATGCGCTGGTGCCGCAGGCGATCGTCGACATGGGCCGCCGCGACGCCGA
GCGGCTGTCGGTCGGCAAGCGCAAGGGCTGCCACACCAAGTCGCAGGATGAGATCAATCATTTGCTGGTGAGGCTCGCCG
GCGAGGGCAAGCGTGTCGTTCGGCTGAAATCGGGGGATCCTCTCGTTTACGGTCGGGCCGGTGAGGAAATGGCGGCGATG
CGCGAGGCGGGCATCAGCTACGAGGTGGTGCCGGGGATCACGTCCGCCTTTGCTGCGGCCGCCGATTTCGAGTTGCCGCT
GACACTGCGCGGCGTGGCGTCGTCACTGGTTTTCACGACCGGGCACGACTTGACCGGCAGCGTCCTTCCCGATTGGGCGC
GGCTGGCGGTTTCCGGCGCGACGATCGCGGTCTATATGGGGCGCACTGTTGCGGCGTCCGTCGCTTCGCGACTCATGCAG
GCGGGCCTGCCGCAGGACACGACCGTCGCGGTCATCGAAAATGCGAGCCGCGCCGAGCGACGCCTACTTCACGGAACCTT
GCGTGATCTCCCTGACCTCGAGACCCGCACGGAACTGGACGGGCCGGTCATGGTGATCATCGGCGAAGCGGTCGCGGGCG
CGAATTTCGAACGGTCCGAGCCGCTGGCGGCGGCCCGGGGCAATGGCAGCGGTGCCGTCGAGGCTTCGGCGAAACGCGAA
ACGATTTGGAATTGA

Upstream 100 bases:

>100_bases
CAATACGTGGGCGGAAAAGCATTGAAATTTCTGTTCCTTCATTTGTTGCCGGCCATAGGGGATATTCGCCGCAGCCTGCA
AAAGGAGCCGACCCTTCGTT

Downstream 100 bases:

>100_bases
GGACGAGGCGATGGTGGAAAAGGTTCTGACGGCAAATCGTCTGGCGGATGGCATATCCGTCTGGCTTGACGCCTCCGGCA
ATTGGGTGGAGTCGCTTCAG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPQKLSVFPAFFRVAQKSVAVFGNGDEAFAKVRLLLSTEARIVAYADQPEAAFEAFLNANGIDTIRKPFDARQVEGAVLV
FAATGDAAFDRIIVAAARERKIPANAVDQPDYCDFLTPALVNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGIVAS
LAASYRDAVDRLVPRGVARRVFWRRFFSGPVADHVALGDLASARREASKLLDAAGGVRGHVWLVGAGPGAEDLLTLRAQR
VMMEADAIVYDALVPQAIVDMGRRDAERLSVGKRKGCHTKSQDEINHLLVRLAGEGKRVVRLKSGDPLVYGRAGEEMAAM
REAGISYEVVPGITSAFAAAADFELPLTLRGVASSLVFTTGHDLTGSVLPDWARLAVSGATIAVYMGRTVAASVASRLMQ
AGLPQDTTVAVIENASRAERRLLHGTLRDLPDLETRTELDGPVMVIIGEAVAGANFERSEPLAAARGNGSGAVEASAKRE
TIWN

Sequences:

>Translated_484_residues
MPQKLSVFPAFFRVAQKSVAVFGNGDEAFAKVRLLLSTEARIVAYADQPEAAFEAFLNANGIDTIRKPFDARQVEGAVLV
FAATGDAAFDRIIVAAARERKIPANAVDQPDYCDFLTPALVNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGIVAS
LAASYRDAVDRLVPRGVARRVFWRRFFSGPVADHVALGDLASARREASKLLDAAGGVRGHVWLVGAGPGAEDLLTLRAQR
VMMEADAIVYDALVPQAIVDMGRRDAERLSVGKRKGCHTKSQDEINHLLVRLAGEGKRVVRLKSGDPLVYGRAGEEMAAM
REAGISYEVVPGITSAFAAAADFELPLTLRGVASSLVFTTGHDLTGSVLPDWARLAVSGATIAVYMGRTVAASVASRLMQ
AGLPQDTTVAVIENASRAERRLLHGTLRDLPDLETRTELDGPVMVIIGEAVAGANFERSEPLAAARGNGSGAVEASAKRE
TIWN
>Mature_483_residues
PQKLSVFPAFFRVAQKSVAVFGNGDEAFAKVRLLLSTEARIVAYADQPEAAFEAFLNANGIDTIRKPFDARQVEGAVLVF
AATGDAAFDRIIVAAARERKIPANAVDQPDYCDFLTPALVNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGIVASL
AASYRDAVDRLVPRGVARRVFWRRFFSGPVADHVALGDLASARREASKLLDAAGGVRGHVWLVGAGPGAEDLLTLRAQRV
MMEADAIVYDALVPQAIVDMGRRDAERLSVGKRKGCHTKSQDEINHLLVRLAGEGKRVVRLKSGDPLVYGRAGEEMAAMR
EAGISYEVVPGITSAFAAAADFELPLTLRGVASSLVFTTGHDLTGSVLPDWARLAVSGATIAVYMGRTVAASVASRLMQA
GLPQDTTVAVIENASRAERRLLHGTLRDLPDLETRTELDGPVMVIIGEAVAGANFERSEPLAAARGNGSGAVEASAKRET
IWN

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=445, Percent_Identity=38.4269662921348, Blast_Score=299, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6322922, Length=257, Percent_Identity=29.5719844357977, Blast_Score=110, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 51425; Mature: 51294

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQKLSVFPAFFRVAQKSVAVFGNGDEAFAKVRLLLSTEARIVAYADQPEAAFEAFLNAN
CCCCCHHHHHHHHHHHHCEEEECCCHHHHHHHHHEEECCCEEEEEECCCHHHHHHHHCCC
GIDTIRKPFDARQVEGAVLVFAATGDAAFDRIIVAAARERKIPANAVDQPDYCDFLTPAL
CCHHHHCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
VNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGIVASLAASYRDAVDRLVPRGVARR
HCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VFWRRFFSGPVADHVALGDLASARREASKLLDAAGGVRGHVWLVGAGPGAEDLLTLRAQR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
VMMEADAIVYDALVPQAIVDMGRRDAERLSVGKRKGCHTKSQDEINHLLVRLAGEGKRVV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEE
RLKSGDPLVYGRAGEEMAAMREAGISYEVVPGITSAFAAAADFELPLTLRGVASSLVFTT
EEECCCCEEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCEEHHHHHHHHEEEC
GHDLTGSVLPDWARLAVSGATIAVYMGRTVAASVASRLMQAGLPQDTTVAVIENASRAER
CCCCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHH
RLLHGTLRDLPDLETRTELDGPVMVIIGEAVAGANFERSEPLAAARGNGSGAVEASAKRE
HHHHHHHHCCCCCCHHHCCCCCEEEEEECHHCCCCCCCCCCCEEECCCCCCCEECCCCCC
TIWN
CCCC
>Mature Secondary Structure 
PQKLSVFPAFFRVAQKSVAVFGNGDEAFAKVRLLLSTEARIVAYADQPEAAFEAFLNAN
CCCCHHHHHHHHHHHHCEEEECCCHHHHHHHHHEEECCCEEEEEECCCHHHHHHHHCCC
GIDTIRKPFDARQVEGAVLVFAATGDAAFDRIIVAAARERKIPANAVDQPDYCDFLTPAL
CCHHHHCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
VNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGIVASLAASYRDAVDRLVPRGVARR
HCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VFWRRFFSGPVADHVALGDLASARREASKLLDAAGGVRGHVWLVGAGPGAEDLLTLRAQR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
VMMEADAIVYDALVPQAIVDMGRRDAERLSVGKRKGCHTKSQDEINHLLVRLAGEGKRVV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEE
RLKSGDPLVYGRAGEEMAAMREAGISYEVVPGITSAFAAAADFELPLTLRGVASSLVFTT
EEECCCCEEECCCCHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCCEEHHHHHHHHEEEC
GHDLTGSVLPDWARLAVSGATIAVYMGRTVAASVASRLMQAGLPQDTTVAVIENASRAER
CCCCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHH
RLLHGTLRDLPDLETRTELDGPVMVIIGEAVAGANFERSEPLAAARGNGSGAVEASAKRE
HHHHHHHHCCCCCCHHHCCCCCEEEEEECHHCCCCCCCCCCCEEECCCCCCCEECCCCCC
TIWN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA