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The map label for this gene is tpiA [H]

Identifier: 150396288

GI number: 150396288

Start: 1135120

End: 1135890

Strand: Direct

Name: tpiA [H]

Synonym: Smed_1067

Alternate gene names: 150396288

Gene position: 1135120-1135890 (Clockwise)

Preceding gene: 150396285

Following gene: 150396289

Centisome position: 30.01

GC content: 63.68

Gene sequence:

>771_bases
ATGACGCCCGATATCCGCCCGCTCGTCGCCGGCAATTGGAAGATGAATGGAACGCGTGCGTCCCTGGACCAGATCAAGGC
AATCGCCGAGGGCGTGAAAGGCGGTCTTTCCGCGAGGGTGGACGCCCTTATCTGCCCGCCGGCAACCTTGCTCTACGTCG
CGACGGCGCTTTGCGACGACAGCCCGTTGTTGATCGGCGCGCAGGACTGCCATCAGAAACCGTCGGGAGCCCATACCGGC
GAAGTCTCTGCCGAAATGATCGCGGATTGCTTCGGGACCCACGTGATCGTCGGCCATTCAGAGCGGCGCAGCGATCATGG
GGAGGGAGACGCCCTGGTTTGCGCCAAGACCGAAGCCGCGCACGGCGCCGGTCTGGTCGCCATCGTATGCATCGGCGAGA
CGGAAGGGGAGCGCAAGGAGGGCCGGACGCTCGATATTCTGAAGCGCCAGCTCGCCGGAAGCCTCCCGGATCAGGCGACG
GCCGAAAACACGGTAATCGCATATGAGCCCGTCTGGGCGATCGGCACCGGTCTCACGCCCACTGCATCCGACGTCGAGGA
GGCCCATGCCTTCATGCGCCGCGAACTCGTGGCGCGCTTCGGCGCGGAAGGCAGCAAGATGCGGATTCTCTATGGAGGGT
CCGTCAAACCCTCGAATGCCAGGGAACTCATGGGGGTCACCAATGTCGACGGCGCCCTGATCGGCGGCGCGAGCTTGAAA
GCGGATGATTTCCTCGCCATCTACAGGGCCTATGAAGAATTGACCGCCTGA

Upstream 100 bases:

>100_bases
GCCGCCGAGGCCGGAATAATCCGATCAGGTGCTTTTTTTTACGCGAGCGATTTGCTACGCCGCTTGCCAAGACGAAGTGA
CCTGATCTGGAGGAGTTCTC

Downstream 100 bases:

>100_bases
TTGCCGCGGGACTTGGAATAGCTGGCGGCTTGGTATAAAGAGGCGCCAAATTTGACGCGGCTCCACGATGAGGCCGGCGT
CGTTCGATTCGTATGCCCCG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 1; Triose-phosphate isomerase 1 [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTPDIRPLVAGNWKMNGTRASLDQIKAIAEGVKGGLSARVDALICPPATLLYVATALCDDSPLLIGAQDCHQKPSGAHTG
EVSAEMIADCFGTHVIVGHSERRSDHGEGDALVCAKTEAAHGAGLVAIVCIGETEGERKEGRTLDILKRQLAGSLPDQAT
AENTVIAYEPVWAIGTGLTPTASDVEEAHAFMRRELVARFGAEGSKMRILYGGSVKPSNARELMGVTNVDGALIGGASLK
ADDFLAIYRAYEELTA

Sequences:

>Translated_256_residues
MTPDIRPLVAGNWKMNGTRASLDQIKAIAEGVKGGLSARVDALICPPATLLYVATALCDDSPLLIGAQDCHQKPSGAHTG
EVSAEMIADCFGTHVIVGHSERRSDHGEGDALVCAKTEAAHGAGLVAIVCIGETEGERKEGRTLDILKRQLAGSLPDQAT
AENTVIAYEPVWAIGTGLTPTASDVEEAHAFMRRELVARFGAEGSKMRILYGGSVKPSNARELMGVTNVDGALIGGASLK
ADDFLAIYRAYEELTA
>Mature_255_residues
TPDIRPLVAGNWKMNGTRASLDQIKAIAEGVKGGLSARVDALICPPATLLYVATALCDDSPLLIGAQDCHQKPSGAHTGE
VSAEMIADCFGTHVIVGHSERRSDHGEGDALVCAKTEAAHGAGLVAIVCIGETEGERKEGRTLDILKRQLAGSLPDQATA
ENTVIAYEPVWAIGTGLTPTASDVEEAHAFMRRELVARFGAEGSKMRILYGGSVKPSNARELMGVTNVDGALIGGASLKA
DDFLAIYRAYEELTA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=38.7351778656126, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=38.7351778656126, Blast_Score=159, Evalue=2e-39,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=41.3654618473896, Blast_Score=184, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17536593, Length=244, Percent_Identity=44.2622950819672, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320255, Length=235, Percent_Identity=45.531914893617, Blast_Score=196, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28572008, Length=251, Percent_Identity=43.8247011952191, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI28572006, Length=251, Percent_Identity=43.8247011952191, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI28572004, Length=251, Percent_Identity=43.8247011952191, Blast_Score=174, Evalue=4e-44,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26883; Mature: 26752

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDIRPLVAGNWKMNGTRASLDQIKAIAEGVKGGLSARVDALICPPATLLYVATALCDD
CCCCCCEEEECCEEECCCHHHHHHHHHHHHHHCCCCHHCEEEEECCCHHHHHHHHHHCCC
SPLLIGAQDCHQKPSGAHTGEVSAEMIADCFGTHVIVGHSERRSDHGEGDALVCAKTEAA
CCEEEECHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHCCCCCCCCEEEEEECCCC
HGAGLVAIVCIGETEGERKEGRTLDILKRQLAGSLPDQATAENTVIAYEPVWAIGTGLTP
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCHHEECCCCCC
TASDVEEAHAFMRRELVARFGAEGSKMRILYGGSVKPSNARELMGVTNVDGALIGGASLK
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCEEECCCCCC
ADDFLAIYRAYEELTA
CHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPDIRPLVAGNWKMNGTRASLDQIKAIAEGVKGGLSARVDALICPPATLLYVATALCDD
CCCCCEEEECCEEECCCHHHHHHHHHHHHHHCCCCHHCEEEEECCCHHHHHHHHHHCCC
SPLLIGAQDCHQKPSGAHTGEVSAEMIADCFGTHVIVGHSERRSDHGEGDALVCAKTEAA
CCEEEECHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHCCCCCCCCEEEEEECCCC
HGAGLVAIVCIGETEGERKEGRTLDILKRQLAGSLPDQATAENTVIAYEPVWAIGTGLTP
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCHHEECCCCCC
TASDVEEAHAFMRRELVARFGAEGSKMRILYGGSVKPSNARELMGVTNVDGALIGGASLK
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCEEECCCCCC
ADDFLAIYRAYEELTA
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]