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The map label for this gene is thrS [H]

Identifier: 150396275

GI number: 150396275

Start: 1121556

End: 1123538

Strand: Direct

Name: thrS [H]

Synonym: Smed_1054

Alternate gene names: 150396275

Gene position: 1121556-1123538 (Clockwise)

Preceding gene: 150396274

Following gene: 150396277

Centisome position: 29.66

GC content: 61.27

Gene sequence:

>1983_bases
ATGTCGCATTCCGTTTCCCTTACATTCCCCGATGGCTCCGTGCGAGAATTCGCCCCCGGCACGACCGGCCGCGATGTTGC
GGAATCGATCTCGAAGTCGCTCGCCAAAAAGTCCGTCGCGATCGCCATCGACGGCGAGCTGCGTGACCTCTCGGATCCCG
TGACGGAAGGCAGGATTGAGATCGTGACGCGCGAGGACAAGCGCGCGCTAGAGCTCATCCGCCACGACGCGGCCCATGTC
ATGGCGGAAGCCGTCCAGGAGTTGTGGCCGGGAACGCAGGTGACCATTGGCCCGGTCATCGACAATGGTTTCTATTACGA
CTTCGCAAAGAACGAGCCCTTCACGCCCGACGACCTGCCGGTCATCGAGAAGAGGATGAGGGAGATCATCGCGCGCAACA
AGCCTTTCACCAAGGAAGTCTGGTCGCGCGACAAGGCCAAGGAGGTCTTTGCCGTCAAGGGCGAGAGTTACAAGGTTGAA
CTCGTCGACGCTATCCCCGAAGGCCAGGACCTGAAGATTTATTACCAGGGCGACTGGTTCGATCTCTGCCGGGGGCCACA
TATGGCTTCGACGGGCCAGATCGGCACCGCCTTCAAATTGATGAAGGTCGCCGGCGCCTACTGGCGTGGCGACAGCAACA
ATCCGATGCTGACGCGCATCTACGGCACCGCCTGGCACACGCAGGAGGAGCTCGATCAGTATCTGCACGTGCTGGCCGAG
GCCGAAAAGCGCGACCATCGCCGGCTCGGCCGCGAGATGGACCTGTTCCATTTCCAGGAGGAAGGACCGGGCGTGGTCTT
CTGGCACGGAAAGGGCTGGCGCATCTTCCAGAGCCTCGTCGCCTATATGCGCCGCCGCCTCGAAGGCGATTATCAGGAGG
TCAACGCTCCGCAGGTGCTCGACAAGTCTCTCTGGGAGACCTCCGGCCACTGGGGATGGTATCGTGACAACATGTTCAAG
GTGACTGTCGCCGGGGACGATACGGATGACGATCGCGTCTTCGCGCTGAAGCCGATGAATTGCCCCGGGCACATCCAGAT
CTTCAAGCATGGCTTGAAGTCTTACCGGGAACTACCTGTAAGGCTGGCAGAATTCGGTGCCGTCCATCGCTACGAGCCAT
CAGGTGCGCTGCACGGCCTGATGCGCGTGCGCGGCTTCACCCAGGACGACGCGCACATCTTCTGCACCGATGAGCAGATG
GCGGCCGAATGCCTGAAGATCAACGATCTTATCCTCTCCGTCTATGAGGACTTCGGCTTCAAGGAGATCGTCGTGAAGCT
GTCGACGCGGCCGGAAAAGCGTGTGGGCTCCGATGAACTGTGGGACCGCGCCGAGGCGGTGATGACGGAGGTCTTGAAGA
CGATCGAGGCGCAGTCCGAGGGCCGCATCAAGACCGGCATCCTGCCGGGCGAGGGTGCGTTCTACGGTCCGAAATTCGAA
TATACGCTGAAGGACGCAATCGGCCGCGAATGGCAGTGCGGAACGACGCAGGTCGATTTCAATCTGCCGGAGCGCTTCGG
CGCCTTCTACATCGACAGCGAATCCGAGAAGCGTCAGCCGGTCATGATCCATCGCGCCATTTGCGGGTCGATGGAGCGTT
TCCTCGGCATCCTGCTCGAGAATTTTGCGGGCCATATGCCGCTGTGGATCTCGCCTCTGCAGGTGGTGGTCGCAACGATC
ACCTCGGAAGCGGATGATTATGGCCGCGAAGTGGCCGAACGCTTGCGGGACGCCGGCCTGACGGTCGAGACCGATTTCCG
AAACGAGAAGATCAACTACAAGGTCCGCGAACACTCGGTAACAAAGGTTCCGGTGATCGTCGTCTGCGGCAAGCGGGAAG
CAGAGGAACGTTCCGTCAACATCCGTCGCCTGGGCTCCCAGGCGCAGACGGCTATGTCGCTCGACGAGGCAGTTGCTTCG
CTCTCCGCCGAAGCCACGGCGCCGGACCTCAAGCGCAAGGCGGAGCGGACCGCCCGCGCCTGA

Upstream 100 bases:

>100_bases
CAACGTGAAAAACCTTTATCAGGCCCGCGTCAATTCACCGGCCAGCATCCGGCAAACCATACCACCCGCATTCGTTGGCG
GGACTGGATAGGAGATCATG

Downstream 100 bases:

>100_bases
CGCGCTTGCCTCTACGCCTGCCAGTCCATTCTCCCGCGTGCGGAGAGAAGGGCCTGGCAGGCGTTCACTCGATCGCCTTC
TCAGGCAATGGCTGCATCAG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MSHSVSLTFPDGSVREFAPGTTGRDVAESISKSLAKKSVAIAIDGELRDLSDPVTEGRIEIVTREDKRALELIRHDAAHV
MAEAVQELWPGTQVTIGPVIDNGFYYDFAKNEPFTPDDLPVIEKRMREIIARNKPFTKEVWSRDKAKEVFAVKGESYKVE
LVDAIPEGQDLKIYYQGDWFDLCRGPHMASTGQIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWHTQEELDQYLHVLAE
AEKRDHRRLGREMDLFHFQEEGPGVVFWHGKGWRIFQSLVAYMRRRLEGDYQEVNAPQVLDKSLWETSGHWGWYRDNMFK
VTVAGDDTDDDRVFALKPMNCPGHIQIFKHGLKSYRELPVRLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHIFCTDEQM
AAECLKINDLILSVYEDFGFKEIVVKLSTRPEKRVGSDELWDRAEAVMTEVLKTIEAQSEGRIKTGILPGEGAFYGPKFE
YTLKDAIGREWQCGTTQVDFNLPERFGAFYIDSESEKRQPVMIHRAICGSMERFLGILLENFAGHMPLWISPLQVVVATI
TSEADDYGREVAERLRDAGLTVETDFRNEKINYKVREHSVTKVPVIVVCGKREAEERSVNIRRLGSQAQTAMSLDEAVAS
LSAEATAPDLKRKAERTARA

Sequences:

>Translated_660_residues
MSHSVSLTFPDGSVREFAPGTTGRDVAESISKSLAKKSVAIAIDGELRDLSDPVTEGRIEIVTREDKRALELIRHDAAHV
MAEAVQELWPGTQVTIGPVIDNGFYYDFAKNEPFTPDDLPVIEKRMREIIARNKPFTKEVWSRDKAKEVFAVKGESYKVE
LVDAIPEGQDLKIYYQGDWFDLCRGPHMASTGQIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWHTQEELDQYLHVLAE
AEKRDHRRLGREMDLFHFQEEGPGVVFWHGKGWRIFQSLVAYMRRRLEGDYQEVNAPQVLDKSLWETSGHWGWYRDNMFK
VTVAGDDTDDDRVFALKPMNCPGHIQIFKHGLKSYRELPVRLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHIFCTDEQM
AAECLKINDLILSVYEDFGFKEIVVKLSTRPEKRVGSDELWDRAEAVMTEVLKTIEAQSEGRIKTGILPGEGAFYGPKFE
YTLKDAIGREWQCGTTQVDFNLPERFGAFYIDSESEKRQPVMIHRAICGSMERFLGILLENFAGHMPLWISPLQVVVATI
TSEADDYGREVAERLRDAGLTVETDFRNEKINYKVREHSVTKVPVIVVCGKREAEERSVNIRRLGSQAQTAMSLDEAVAS
LSAEATAPDLKRKAERTARA
>Mature_659_residues
SHSVSLTFPDGSVREFAPGTTGRDVAESISKSLAKKSVAIAIDGELRDLSDPVTEGRIEIVTREDKRALELIRHDAAHVM
AEAVQELWPGTQVTIGPVIDNGFYYDFAKNEPFTPDDLPVIEKRMREIIARNKPFTKEVWSRDKAKEVFAVKGESYKVEL
VDAIPEGQDLKIYYQGDWFDLCRGPHMASTGQIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWHTQEELDQYLHVLAEA
EKRDHRRLGREMDLFHFQEEGPGVVFWHGKGWRIFQSLVAYMRRRLEGDYQEVNAPQVLDKSLWETSGHWGWYRDNMFKV
TVAGDDTDDDRVFALKPMNCPGHIQIFKHGLKSYRELPVRLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHIFCTDEQMA
AECLKINDLILSVYEDFGFKEIVVKLSTRPEKRVGSDELWDRAEAVMTEVLKTIEAQSEGRIKTGILPGEGAFYGPKFEY
TLKDAIGREWQCGTTQVDFNLPERFGAFYIDSESEKRQPVMIHRAICGSMERFLGILLENFAGHMPLWISPLQVVVATIT
SEADDYGREVAERLRDAGLTVETDFRNEKINYKVREHSVTKVPVIVVCGKREAEERSVNIRRLGSQAQTAMSLDEAVASL
SAEATAPDLKRKAERTARA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=646, Percent_Identity=37.3065015479876, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI38570111, Length=626, Percent_Identity=37.0607028753994, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI20070344, Length=655, Percent_Identity=37.7099236641221, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI1788013, Length=652, Percent_Identity=49.2331288343558, Blast_Score=671, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=646, Percent_Identity=38.0804953560372, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984184, Length=646, Percent_Identity=38.0804953560372, Blast_Score=456, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322112, Length=665, Percent_Identity=40.1503759398496, Blast_Score=453, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322654, Length=402, Percent_Identity=34.8258706467662, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24583839, Length=643, Percent_Identity=38.2581648522551, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24583842, Length=643, Percent_Identity=38.2581648522551, Blast_Score=441, Evalue=1e-123,
Organism=Drosophila melanogaster, GI28574141, Length=643, Percent_Identity=38.2581648522551, Blast_Score=441, Evalue=1e-123,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 74867; Mature: 74736

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSVSLTFPDGSVREFAPGTTGRDVAESISKSLAKKSVAIAIDGELRDLSDPVTEGRIE
CCCEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCCCCEEE
IVTREDKRALELIRHDAAHVMAEAVQELWPGTQVTIGPVIDNGFYYDFAKNEPFTPDDLP
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCCCCH
VIEKRMREIIARNKPFTKEVWSRDKAKEVFAVKGESYKVELVDAIPEGQDLKIYYQGDWF
HHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCEEEEECCCHH
DLCRGPHMASTGQIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWHTQEELDQYLHVLAE
HHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
AEKRDHRRLGREMDLFHFQEEGPGVVFWHGKGWRIFQSLVAYMRRRLEGDYQEVNAPQVL
HHHHHHHHHCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHH
DKSLWETSGHWGWYRDNMFKVTVAGDDTDDDRVFALKPMNCPGHIQIFKHGLKSYRELPV
HHHHHHCCCCCCEEECCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
RLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHIFCTDEQMAAECLKINDLILSVYEDFGF
HHHHHCCCEEECCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCH
KEIVVKLSTRPEKRVGSDELWDRAEAVMTEVLKTIEAQSEGRIKTGILPGEGAFYGPKFE
HHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCEE
YTLKDAIGREWQCGTTQVDFNLPERFGAFYIDSESEKRQPVMIHRAICGSMERFLGILLE
EEHHHHCCCCCCCCCEEEECCCHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NFAGHMPLWISPLQVVVATITSEADDYGREVAERLRDAGLTVETDFRNEKINYKVREHSV
HHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCC
TKVPVIVVCGKREAEERSVNIRRLGSQAQTAMSLDEAVASLSAEATAPDLKRKAERTARA
CCCEEEEEECCCCCHHHCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SHSVSLTFPDGSVREFAPGTTGRDVAESISKSLAKKSVAIAIDGELRDLSDPVTEGRIE
CCEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCCCCEEE
IVTREDKRALELIRHDAAHVMAEAVQELWPGTQVTIGPVIDNGFYYDFAKNEPFTPDDLP
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCCCCH
VIEKRMREIIARNKPFTKEVWSRDKAKEVFAVKGESYKVELVDAIPEGQDLKIYYQGDWF
HHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCEEEEECCCHH
DLCRGPHMASTGQIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWHTQEELDQYLHVLAE
HHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
AEKRDHRRLGREMDLFHFQEEGPGVVFWHGKGWRIFQSLVAYMRRRLEGDYQEVNAPQVL
HHHHHHHHHCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHH
DKSLWETSGHWGWYRDNMFKVTVAGDDTDDDRVFALKPMNCPGHIQIFKHGLKSYRELPV
HHHHHHCCCCCCEEECCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
RLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHIFCTDEQMAAECLKINDLILSVYEDFGF
HHHHHCCCEEECCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCH
KEIVVKLSTRPEKRVGSDELWDRAEAVMTEVLKTIEAQSEGRIKTGILPGEGAFYGPKFE
HHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCEE
YTLKDAIGREWQCGTTQVDFNLPERFGAFYIDSESEKRQPVMIHRAICGSMERFLGILLE
EEHHHHCCCCCCCCCEEEECCCHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NFAGHMPLWISPLQVVVATITSEADDYGREVAERLRDAGLTVETDFRNEKINYKVREHSV
HHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCC
TKVPVIVVCGKREAEERSVNIRRLGSQAQTAMSLDEAVASLSAEATAPDLKRKAERTARA
CCCEEEEEECCCCCHHHCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]