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The map label for this gene is rpoA [H]

Identifier: 150396231

GI number: 150396231

Start: 1081123

End: 1082133

Strand: Direct

Name: rpoA [H]

Synonym: Smed_1010

Alternate gene names: 150396231

Gene position: 1081123-1082133 (Clockwise)

Preceding gene: 150396230

Following gene: 150396232

Centisome position: 28.59

GC content: 57.86

Gene sequence:

>1011_bases
ATGATCCAGAAAAATTGGCAGGAATTGATCAAGCCGAACAAGGTGGAGTTCGCCTCCTCCGGCCGCACCAAGGCAACGTT
GGTAGCGGAACCGCTTGAGCGGGGCTTTGGTCTGACGCTCGGCAACGCGCTTCGTCGCGTGCTTTTGTCGTCGCTGCGCG
GTGCCGCTGTAACCGCGGTTCAGATCGACGGCGTATTGCATGAGTTCTCTTCTATCCCGGGCGTCCGGGAAGACGTGACC
GACATCGTGCTCAACATCAAGGAAATCGCCATCAAGATGGATGGCGACGACGCGAAGCGCATGGTCGTGCGCAAGCAGGG
CCCTGGCGTTGTTACCGCCGGTGACATTCAGACGGTTGGCGATATCGAGATCCTCAACCCGAACCACGTGATCTGCACGC
TCGACGAGGGCGCGGAAATCCGCATGGAGTTCACCGTCAATAACGGCAAGGGCTATGTACCGGCTGACCGTAACCGCTCG
GAAGACGCGCCGATCGGGCTCATTCCGGTGGATAGCCTGTACTCTCCGGTCAAGAAGGTCTCCTACAAGGTGGAAAACAC
CCGTGAAGGCCAGGTTCTCGACTATGACAAGCTGACGATGTCCATCGAGACCGATGGCTCCGTCACGGGCGAAGATGCGA
TCGCGTTCGCGGCCCGCATCCTTCAGGACCAGCTGTCGGTCTTCGTTAACTTCGACGAGCCGCAGAAGGAAACCGAGGAA
GAGGCGGTCACCGAACTCGCCTTCAATCCGGCGCTTCTCAAGAAGGTCGACGAACTGGAGCTTTCCGTCCGCTCGGCCAA
CTGCCTGAAGAACGACAACATCGTCTATATCGGCGACCTCATTCAGAAGACCGAAGCAGAAATGCTCCGCACGCCGAATT
TTGGTCGCAAGTCGCTCAACGAGATCAAGGAAGTTCTCGCTTCCATGGGCCTGCATCTCGGCATGGAAGTGCCGTCCTGG
CCGCCGGAGAACATCGAAGATCTCGCCAAGCGATACGAAGACCAATATTAA

Upstream 100 bases:

>100_bases
TCTGTATTCAACATTCCGGTGAGGACGTCGCGATGTCCTCCCGGTCTTCGGGGCTCGGTTGTCACGATTGGATGGTGGCA
ACGAACGGAAGGCAGAAAAC

Downstream 100 bases:

>100_bases
CCGTTTTAGACTGCAGGCGGATGCCTGCAAGTAAAGGAGAATAGCCATGCGCCACGGTAAAGCCGGCCGCAAGCTGAACA
GAACCGCCAGCCACCGCAAG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MIQKNWQELIKPNKVEFASSGRTKATLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDVT
DIVLNIKEIAIKMDGDDAKRMVVRKQGPGVVTAGDIQTVGDIEILNPNHVICTLDEGAEIRMEFTVNNGKGYVPADRNRS
EDAPIGLIPVDSLYSPVKKVSYKVENTREGQVLDYDKLTMSIETDGSVTGEDAIAFAARILQDQLSVFVNFDEPQKETEE
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPSW
PPENIEDLAKRYEDQY

Sequences:

>Translated_336_residues
MIQKNWQELIKPNKVEFASSGRTKATLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDVT
DIVLNIKEIAIKMDGDDAKRMVVRKQGPGVVTAGDIQTVGDIEILNPNHVICTLDEGAEIRMEFTVNNGKGYVPADRNRS
EDAPIGLIPVDSLYSPVKKVSYKVENTREGQVLDYDKLTMSIETDGSVTGEDAIAFAARILQDQLSVFVNFDEPQKETEE
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPSW
PPENIEDLAKRYEDQY
>Mature_336_residues
MIQKNWQELIKPNKVEFASSGRTKATLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDVT
DIVLNIKEIAIKMDGDDAKRMVVRKQGPGVVTAGDIQTVGDIEILNPNHVICTLDEGAEIRMEFTVNNGKGYVPADRNRS
EDAPIGLIPVDSLYSPVKKVSYKVENTREGQVLDYDKLTMSIETDGSVTGEDAIAFAARILQDQLSVFVNFDEPQKETEE
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPSW
PPENIEDLAKRYEDQY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=331, Percent_Identity=51.3595166163142, Blast_Score=323, Evalue=9e-90,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 37172; Mature: 37172

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKNWQELIKPNKVEFASSGRTKATLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAV
CCCCCHHHHCCCCCEEECCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
QIDGVLHEFSSIPGVREDVTDIVLNIKEIAIKMDGDDAKRMVVRKQGPGVVTAGDIQTVG
EHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCHHHHEEEEECCCCEEEECCCCCCC
DIEILNPNHVICTLDEGAEIRMEFTVNNGKGYVPADRNRSEDAPIGLIPVDSLYSPVKKV
CEEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
SYKVENTREGQVLDYDKLTMSIETDGSVTGEDAIAFAARILQDQLSVFVNFDEPQKETEE
HEEECCCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHH
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLN
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHH
EIKEVLASMGLHLGMEVPSWPPENIEDLAKRYEDQY
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIQKNWQELIKPNKVEFASSGRTKATLVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAV
CCCCCHHHHCCCCCEEECCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
QIDGVLHEFSSIPGVREDVTDIVLNIKEIAIKMDGDDAKRMVVRKQGPGVVTAGDIQTVG
EHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCHHHHEEEEECCCCEEEECCCCCCC
DIEILNPNHVICTLDEGAEIRMEFTVNNGKGYVPADRNRSEDAPIGLIPVDSLYSPVKKV
CEEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
SYKVENTREGQVLDYDKLTMSIETDGSVTGEDAIAFAARILQDQLSVFVNFDEPQKETEE
HEEECCCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHH
EAVTELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLN
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHH
EIKEVLASMGLHLGMEVPSWPPENIEDLAKRYEDQY
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]