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The map label for this gene is glyS [H]

Identifier: 150395691

GI number: 150395691

Start: 503291

End: 505456

Strand: Direct

Name: glyS [H]

Synonym: Smed_0466

Alternate gene names: 150395691

Gene position: 503291-505456 (Clockwise)

Preceding gene: 150395690

Following gene: 150395692

Centisome position: 13.31

GC content: 62.51

Gene sequence:

>2166_bases
ATGCCCGATCTTCTCATTGAACTGCGCTCCGAAGAAATCCCCGCCCGCATGCAGCGCAAAGCGGCCGGAGATTTGAAGAA
GCTCGTGACCGACGCTCTGGTCGAGGCGGGACTGACCTACGAAGGCGCGCGGGAATACTGGACGCCGCGGCGGCTGACGC
TCGACATTCGCGGCCTGAATGCGCGCTCCGCCGATATGCGCGAGGAGCGCAAGGGGCCGCGCATCGATGCGAACGATAAG
GCGATCGAAGGCTTTCTGCGCGCGGCAGGACTCTCGTCCATCAGTGAAGCTCATGTCCATAATGATCCGAAGAAGGGCGA
CTTCTATGTCGCGCACATCGTCAGGCGCGGCCGGGAGGCGGAAGAGATCGTCGCCGACGTGATGCCGGCGATCATTCGCG
ACTTTCCCTGGCCGAAATCCATGCGCTCGGGTGCCGCTTCGGCGATACCGGGCAGCCTTCGCTGGGTCCGTCCGCTGCAG
TCGATCGTCTGCACCTTCGGCGCGGAACATGACGAGACGAAGGTGATCCCCTTCGAGGTCGACGGCATCGTCGCGTCCAA
CATCACCTACGGCCATCGCTTCCACGCACCGGAGGCGATCGCCGTCCGCCGCTTCGCCGATTACGCCGAGAAGCTCGGAA
AGGCGAAGGTGGTGATCGACGCGGAGCGCCGCAAGGAAATCATCTCCGCCGACGCCCACAACATCGCTTTCGCCAATGGC
CTTGAACTCGTCGAGGATGAGGGCCTTTTGGAAGAGGTCGCCGGACTGGTCGAATGGCCGAAGGTGCTGATGGGCAGTTT
CGAGGAAAGCTATCTCGCAATTCCGTCGGAAATCATCCGGCTGACGATCAAGACCAACCAGAAATGCTTCGTTACGCGCA
AGCCCGGCGCAGAAACGCTGTCCAACAGGTTCATCCTCGTTTCGAATATCGAGGCGAAGGATGGCGGCAAGGAAATCGTC
CATGGCAACGGAAAGGTCGTACGGGCGCGGCTCTCCGACGCAGCCCACTTCTGGAAGCGCGACCAGGGCGATTTGCCGGA
TCTCGAAACGCTGGCCGCATCTGCCGGGAAGTTCGGGCTCGATCTGAAAAAACCTCTCGACCAGCGGATGGCCAAGCTGG
ACGCCTTGAACGTGACCTTTCATGCCAAGCTCGGCACGCAAGGCGAGCGCGTGGCCCGTATCCGTTCGCTCGCTGCAGCG
CTCGCCAAGATCACCGGTGCCGACCCGGCATCCGTCGCGCGCGCGGCCGTGCTCGCCAAGGCTGACCTGCGCACCGAGGC
CGTCGGCGAGTTTCCGGAACTGCAGGGCATCATGGGCCATAAATATGCAGTATTGCAGGGTGAAAGCGCGGCCGTCGCCA
ATGCGATCGAAGACCACTACAAGCCTCAGGGCCCGTCGGACCACGTGCCGACCGACCCCGTCTCGGTTTCGGTCGCACTT
GCCGACAAGCTCGATACCCTCGTCGGTTTCTGGGCGATCGACGAAAGGCCGACCGGTTCGAAGGACCCCTATGCATTGCG
TCGGGCGGCCCTTGGCGTGATCCGCCTCATTCTCGAAGGAAGGGCTCGGCTGCCGCTCCTGCCCTGTTTCGACGTCGCTC
TGGCCTCACTCAAACTGCAGCGACCGGCCATGGCGGCGGATGTTTCCGGTGACCTGCTGGCTTTCTTCCATGATCGCCTC
AAGGTTTATCTCCGCGATCTTGGCGCTCGGCACGACCTGATCGACGCGGTTCTTGCGCCGGATGCGGACGACCTGTTGAT
GATTGCTCGGCGCGTTGAGGCGCTGACGGCCTTCATCACGGCCGAGGAGGGCAGAAACCTTCTGGCCGGCACGAAGCGCG
CGACGCAAATTCTCGCTGCCGAAGAGAAGAAGGGCACGGAAGTCGCAGCCTCGGTTGACGAAGAATTGTTCCGGCTCGAT
GCCGAGCGGTTGCTGTATGCCTCTATCAAGGTCGCGTCGGACGGTGCGCGCGCGGCGATCGCCAAGGAAGATTTCCGCTC
GGCGATGGAGGCTCTCTCGAAGTTGCGCGAGCCGGTCGACGCCTTTTTCAACGATGTGCTCGTCAATGACGAGGACAAGG
CGATCCGTGCAAACCGCCTCGCACTTCTGGGGCTCATCCGCTCGGCGACCGGGGAGGTCGCGGATTTCTCGAAGATCTCC
GGGTAA

Upstream 100 bases:

>100_bases
ACATGCCAAAAACAATGGCTTAAAGCGCAGCGCGTGATTTCGCTGGAACGCGACTCGCTTCAAGCTCCCGCGACACCCTT
CCGGAACAGAACAAAGTCTC

Downstream 100 bases:

>100_bases
CGCCGGAGTGGCTCTCGCATCGCCGAGCTTGTCGCGTTACCGGCTGCCGGTTATGACTCGGCGATGATCACGAGGATCCT
CGTCAGTGCCTGCCTTATGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MPDLLIELRSEEIPARMQRKAAGDLKKLVTDALVEAGLTYEGAREYWTPRRLTLDIRGLNARSADMREERKGPRIDANDK
AIEGFLRAAGLSSISEAHVHNDPKKGDFYVAHIVRRGREAEEIVADVMPAIIRDFPWPKSMRSGAASAIPGSLRWVRPLQ
SIVCTFGAEHDETKVIPFEVDGIVASNITYGHRFHAPEAIAVRRFADYAEKLGKAKVVIDAERRKEIISADAHNIAFANG
LELVEDEGLLEEVAGLVEWPKVLMGSFEESYLAIPSEIIRLTIKTNQKCFVTRKPGAETLSNRFILVSNIEAKDGGKEIV
HGNGKVVRARLSDAAHFWKRDQGDLPDLETLAASAGKFGLDLKKPLDQRMAKLDALNVTFHAKLGTQGERVARIRSLAAA
LAKITGADPASVARAAVLAKADLRTEAVGEFPELQGIMGHKYAVLQGESAAVANAIEDHYKPQGPSDHVPTDPVSVSVAL
ADKLDTLVGFWAIDERPTGSKDPYALRRAALGVIRLILEGRARLPLLPCFDVALASLKLQRPAMAADVSGDLLAFFHDRL
KVYLRDLGARHDLIDAVLAPDADDLLMIARRVEALTAFITAEEGRNLLAGTKRATQILAAEEKKGTEVAASVDEELFRLD
AERLLYASIKVASDGARAAIAKEDFRSAMEALSKLREPVDAFFNDVLVNDEDKAIRANRLALLGLIRSATGEVADFSKIS
G

Sequences:

>Translated_721_residues
MPDLLIELRSEEIPARMQRKAAGDLKKLVTDALVEAGLTYEGAREYWTPRRLTLDIRGLNARSADMREERKGPRIDANDK
AIEGFLRAAGLSSISEAHVHNDPKKGDFYVAHIVRRGREAEEIVADVMPAIIRDFPWPKSMRSGAASAIPGSLRWVRPLQ
SIVCTFGAEHDETKVIPFEVDGIVASNITYGHRFHAPEAIAVRRFADYAEKLGKAKVVIDAERRKEIISADAHNIAFANG
LELVEDEGLLEEVAGLVEWPKVLMGSFEESYLAIPSEIIRLTIKTNQKCFVTRKPGAETLSNRFILVSNIEAKDGGKEIV
HGNGKVVRARLSDAAHFWKRDQGDLPDLETLAASAGKFGLDLKKPLDQRMAKLDALNVTFHAKLGTQGERVARIRSLAAA
LAKITGADPASVARAAVLAKADLRTEAVGEFPELQGIMGHKYAVLQGESAAVANAIEDHYKPQGPSDHVPTDPVSVSVAL
ADKLDTLVGFWAIDERPTGSKDPYALRRAALGVIRLILEGRARLPLLPCFDVALASLKLQRPAMAADVSGDLLAFFHDRL
KVYLRDLGARHDLIDAVLAPDADDLLMIARRVEALTAFITAEEGRNLLAGTKRATQILAAEEKKGTEVAASVDEELFRLD
AERLLYASIKVASDGARAAIAKEDFRSAMEALSKLREPVDAFFNDVLVNDEDKAIRANRLALLGLIRSATGEVADFSKIS
G
>Mature_720_residues
PDLLIELRSEEIPARMQRKAAGDLKKLVTDALVEAGLTYEGAREYWTPRRLTLDIRGLNARSADMREERKGPRIDANDKA
IEGFLRAAGLSSISEAHVHNDPKKGDFYVAHIVRRGREAEEIVADVMPAIIRDFPWPKSMRSGAASAIPGSLRWVRPLQS
IVCTFGAEHDETKVIPFEVDGIVASNITYGHRFHAPEAIAVRRFADYAEKLGKAKVVIDAERRKEIISADAHNIAFANGL
ELVEDEGLLEEVAGLVEWPKVLMGSFEESYLAIPSEIIRLTIKTNQKCFVTRKPGAETLSNRFILVSNIEAKDGGKEIVH
GNGKVVRARLSDAAHFWKRDQGDLPDLETLAASAGKFGLDLKKPLDQRMAKLDALNVTFHAKLGTQGERVARIRSLAAAL
AKITGADPASVARAAVLAKADLRTEAVGEFPELQGIMGHKYAVLQGESAAVANAIEDHYKPQGPSDHVPTDPVSVSVALA
DKLDTLVGFWAIDERPTGSKDPYALRRAALGVIRLILEGRARLPLLPCFDVALASLKLQRPAMAADVSGDLLAFFHDRLK
VYLRDLGARHDLIDAVLAPDADDLLMIARRVEALTAFITAEEGRNLLAGTKRATQILAAEEKKGTEVAASVDEELFRLDA
ERLLYASIKVASDGARAAIAKEDFRSAMEALSKLREPVDAFFNDVLVNDEDKAIRANRLALLGLIRSATGEVADFSKISG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=725, Percent_Identity=35.3103448275862, Blast_Score=350, Evalue=1e-97,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 78697; Mature: 78566

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLIELRSEEIPARMQRKAAGDLKKLVTDALVEAGLTYEGAREYWTPRRLTLDIRGLN
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEECCC
ARSADMREERKGPRIDANDKAIEGFLRAAGLSSISEAHVHNDPKKGDFYVAHIVRRGREA
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHCCCHH
EEIVADVMPAIIRDFPWPKSMRSGAASAIPGSLRWVRPLQSIVCTFGAEHDETKVIPFEV
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
DGIVASNITYGHRFHAPEAIAVRRFADYAEKLGKAKVVIDAERRKEIISADAHNIAFANG
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCEEECCC
LELVEDEGLLEEVAGLVEWPKVLMGSFEESYLAIPSEIIRLTIKTNQKCFVTRKPGAETL
CHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHEEEEEECCCEEEEEECCCHHHH
SNRFILVSNIEAKDGGKEIVHGNGKVVRARLSDAAHFWKRDQGDLPDLETLAASAGKFGL
CCCEEEEEECCCCCCCCEEEECCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC
DLKKPLDQRMAKLDALNVTFHAKLGTQGERVARIRSLAAALAKITGADPASVARAAVLAK
CHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ADLRTEAVGEFPELQGIMGHKYAVLQGESAAVANAIEDHYKPQGPSDHVPTDPVSVSVAL
HHHHHHHHCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEH
ADKLDTLVGFWAIDERPTGSKDPYALRRAALGVIRLILEGRARLPLLPCFDVALASLKLQ
HHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
RPAMAADVSGDLLAFFHDRLKVYLRDLGARHDLIDAVLAPDADDLLMIARRVEALTAFIT
CCCHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AEEGRNLLAGTKRATQILAAEEKKGTEVAASVDEELFRLDAERLLYASIKVASDGARAAI
HHCCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHH
AKEDFRSAMEALSKLREPVDAFFNDVLVNDEDKAIRANRLALLGLIRSATGEVADFSKIS
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCC
G
C
>Mature Secondary Structure 
PDLLIELRSEEIPARMQRKAAGDLKKLVTDALVEAGLTYEGAREYWTPRRLTLDIRGLN
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEECCC
ARSADMREERKGPRIDANDKAIEGFLRAAGLSSISEAHVHNDPKKGDFYVAHIVRRGREA
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHCCCHH
EEIVADVMPAIIRDFPWPKSMRSGAASAIPGSLRWVRPLQSIVCTFGAEHDETKVIPFEV
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
DGIVASNITYGHRFHAPEAIAVRRFADYAEKLGKAKVVIDAERRKEIISADAHNIAFANG
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCEEECCC
LELVEDEGLLEEVAGLVEWPKVLMGSFEESYLAIPSEIIRLTIKTNQKCFVTRKPGAETL
CHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHEEEEEECCCEEEEEECCCHHHH
SNRFILVSNIEAKDGGKEIVHGNGKVVRARLSDAAHFWKRDQGDLPDLETLAASAGKFGL
CCCEEEEEECCCCCCCCEEEECCCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCC
DLKKPLDQRMAKLDALNVTFHAKLGTQGERVARIRSLAAALAKITGADPASVARAAVLAK
CHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ADLRTEAVGEFPELQGIMGHKYAVLQGESAAVANAIEDHYKPQGPSDHVPTDPVSVSVAL
HHHHHHHHCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEH
ADKLDTLVGFWAIDERPTGSKDPYALRRAALGVIRLILEGRARLPLLPCFDVALASLKLQ
HHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
RPAMAADVSGDLLAFFHDRLKVYLRDLGARHDLIDAVLAPDADDLLMIARRVEALTAFIT
CCCHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AEEGRNLLAGTKRATQILAAEEKKGTEVAASVDEELFRLDAERLLYASIKVASDGARAAI
HHCCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHH
AKEDFRSAMEALSKLREPVDAFFNDVLVNDEDKAIRANRLALLGLIRSATGEVADFSKIS
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]