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The map label for this gene is waaA [H]

Identifier: 150395649

GI number: 150395649

Start: 457632

End: 458951

Strand: Direct

Name: waaA [H]

Synonym: Smed_0424

Alternate gene names: 150395649

Gene position: 457632-458951 (Clockwise)

Preceding gene: 150395648

Following gene: 150395650

Centisome position: 12.1

GC content: 63.11

Gene sequence:

>1320_bases
ATGAGTAGCGTGCGTGCACGGATGGCACTTGCTGGGTACCGCTGGCTTGGAACTGCCATTTATCCCTTCTTCTGGTCCTA
TCTTGCTCTGCGCGCGGCAAAGGGCAAGGAGGACCCGGCACGCAGGCGGGAGCGCTATGGCTATCCGAGCGCGCCGCGTC
CGCAGGGTCCCCTCGTCTGGTTCCACGCCGCCAGCGTAGGAGAGACGAATGCGGTTACTCCGCTGATCAAGGAAATCCGT
CGTCGCGGCATAGCCGTGGTGCTGACCACCGGCACGACGACCTCGGCGCGGGTCGCGGCAGAGCGATTGGGCTCGGCCGT
CGTGCATCAATACGTACCGCTCGATTTCAAGCCCGCGGTAAGCCGCTTCCTGGAATATTGGCAGCCGGACCTGGCGATCA
TCGCCGAATCGGAAATCTGGCCGATGACGATCATCGAACTCGGCAGGCGCCACATTCCGCAGGTACTGGTCAATGGCCGT
CTTTCGGATCGCACTTTCGCCCGCTGGCGGCGGCGCCCGTCCTTGGCTGATGCTCTCTTTGAAAACCTCGCGCTCGTCAT
CGCCCAGTCCGATGTGGATGCGGAGCGGTTTCGCACGCTCGGCGCGCTCCCGGTCACCGTTTCCGGCAATCTCAAGGTGG
ACAATGAGGCTCCGCCCCACGATCCCCGGGATCTGCGCGAATACCGGCAACAGATCGGCGCGCGAAAGACTTGGGCTGCA
ATCTCGACCTTCGAGGGAGAGGAGAATGCGGCCGGGACGGTCCATCAGGCGCTGAAGGAAAGAACCGGGCTGCTTACGAT
CATCGTCCCGCGCCACCCGGAGCGTTGCGATGCCATCGAGTCGGCCCTGGTTGCCAAGGGGATGAAGGTCGCGCGCCGCA
CGCGCGGCGATCCGGTCACGCCTGACATAGACATTCTCCTCGGCGATACGATCGGCGAGATGGGGCTCTTTCTCAGACTG
ACTGAGGTCGCTTTCGTCGGACGCTCGCTTTTCGCGGAAGGCGGGCAGAACCCGCTGGAACCGGCTATGCTCGGCTGCGC
CGTTCTTTCGGGCGGGAACGTTCAGAATTTCCGCGAGACCTACCAGATGCTCGCCAAGAACGGCAGTGCCAAGATCGTGC
GCGATACGGAGATGCTGGCCAAGGGGGTGAACTACCTGCTTGCCAACGACGACATGCGTCGTTCGATGATCGACGCCGGT
CTTGAAACCGTGCAACAGATGCGCGGCGCGCTCTCGGCGACGATGAAAGGGCTCGAGCCTTATATCAACCCGCTGGTAGT
GAAGGCGCGGCTGGAGCCGCGCCCAAAGCCCGAAGCCTGA

Upstream 100 bases:

>100_bases
AATGCGCATATGCGCTATTTCATCGTTCACATGCATCGGCTGCTCGATCCGGAAGTCAAATGATGCCGACGGCGCCGGAC
GCAGCTGAGGACGAGCGTTC

Downstream 100 bases:

>100_bases
TTTCCGGAAGGGACCATGCCCGTATTGAAGCCGATCGCCGGCATATTGTTCGACAAGGACGGTACCCTTCTCGATTATGT
GAAGAGCTGGGTCCCGGTGA

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSSVRARMALAGYRWLGTAIYPFFWSYLALRAAKGKEDPARRRERYGYPSAPRPQGPLVWFHAASVGETNAVTPLIKEIR
RRGIAVVLTTGTTTSARVAAERLGSAVVHQYVPLDFKPAVSRFLEYWQPDLAIIAESEIWPMTIIELGRRHIPQVLVNGR
LSDRTFARWRRRPSLADALFENLALVIAQSDVDAERFRTLGALPVTVSGNLKVDNEAPPHDPRDLREYRQQIGARKTWAA
ISTFEGEENAAGTVHQALKERTGLLTIIVPRHPERCDAIESALVAKGMKVARRTRGDPVTPDIDILLGDTIGEMGLFLRL
TEVAFVGRSLFAEGGQNPLEPAMLGCAVLSGGNVQNFRETYQMLAKNGSAKIVRDTEMLAKGVNYLLANDDMRRSMIDAG
LETVQQMRGALSATMKGLEPYINPLVVKARLEPRPKPEA

Sequences:

>Translated_439_residues
MSSVRARMALAGYRWLGTAIYPFFWSYLALRAAKGKEDPARRRERYGYPSAPRPQGPLVWFHAASVGETNAVTPLIKEIR
RRGIAVVLTTGTTTSARVAAERLGSAVVHQYVPLDFKPAVSRFLEYWQPDLAIIAESEIWPMTIIELGRRHIPQVLVNGR
LSDRTFARWRRRPSLADALFENLALVIAQSDVDAERFRTLGALPVTVSGNLKVDNEAPPHDPRDLREYRQQIGARKTWAA
ISTFEGEENAAGTVHQALKERTGLLTIIVPRHPERCDAIESALVAKGMKVARRTRGDPVTPDIDILLGDTIGEMGLFLRL
TEVAFVGRSLFAEGGQNPLEPAMLGCAVLSGGNVQNFRETYQMLAKNGSAKIVRDTEMLAKGVNYLLANDDMRRSMIDAG
LETVQQMRGALSATMKGLEPYINPLVVKARLEPRPKPEA
>Mature_438_residues
SSVRARMALAGYRWLGTAIYPFFWSYLALRAAKGKEDPARRRERYGYPSAPRPQGPLVWFHAASVGETNAVTPLIKEIRR
RGIAVVLTTGTTTSARVAAERLGSAVVHQYVPLDFKPAVSRFLEYWQPDLAIIAESEIWPMTIIELGRRHIPQVLVNGRL
SDRTFARWRRRPSLADALFENLALVIAQSDVDAERFRTLGALPVTVSGNLKVDNEAPPHDPRDLREYRQQIGARKTWAAI
STFEGEENAAGTVHQALKERTGLLTIIVPRHPERCDAIESALVAKGMKVARRTRGDPVTPDIDILLGDTIGEMGLFLRLT
EVAFVGRSLFAEGGQNPLEPAMLGCAVLSGGNVQNFRETYQMLAKNGSAKIVRDTEMLAKGVNYLLANDDMRRSMIDAGL
ETVQQMRGALSATMKGLEPYINPLVVKARLEPRPKPEA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=422, Percent_Identity=36.2559241706161, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48646; Mature: 48515

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVRARMALAGYRWLGTAIYPFFWSYLALRAAKGKEDPARRRERYGYPSAPRPQGPLVW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEE
FHAASVGETNAVTPLIKEIRRRGIAVVLTTGTTTSARVAAERLGSAVVHQYVPLDFKPAV
EEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
SRFLEYWQPDLAIIAESEIWPMTIIELGRRHIPQVLVNGRLSDRTFARWRRRPSLADALF
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHH
ENLALVIAQSDVDAERFRTLGALPVTVSGNLKVDNEAPPHDPRDLREYRQQIGARKTWAA
HHHEEEEECCCCCHHHHHHHCCCEEEEECCEEECCCCCCCCHHHHHHHHHHHCCCHHHHH
ISTFEGEENAAGTVHQALKERTGLLTIIVPRHPERCDAIESALVAKGMKVARRTRGDPVT
HHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDIDILLGDTIGEMGLFLRLTEVAFVGRSLFAEGGQNPLEPAMLGCAVLSGGNVQNFRET
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHH
YQMLAKNGSAKIVRDTEMLAKGVNYLLANDDMRRSMIDAGLETVQQMRGALSATMKGLEP
HHHHHCCCCCEEEEHHHHHHHCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YINPLVVKARLEPRPKPEA
HHCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
SSVRARMALAGYRWLGTAIYPFFWSYLALRAAKGKEDPARRRERYGYPSAPRPQGPLVW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEE
FHAASVGETNAVTPLIKEIRRRGIAVVLTTGTTTSARVAAERLGSAVVHQYVPLDFKPAV
EEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
SRFLEYWQPDLAIIAESEIWPMTIIELGRRHIPQVLVNGRLSDRTFARWRRRPSLADALF
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHH
ENLALVIAQSDVDAERFRTLGALPVTVSGNLKVDNEAPPHDPRDLREYRQQIGARKTWAA
HHHEEEEECCCCCHHHHHHHCCCEEEEECCEEECCCCCCCCHHHHHHHHHHHCCCHHHHH
ISTFEGEENAAGTVHQALKERTGLLTIIVPRHPERCDAIESALVAKGMKVARRTRGDPVT
HHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDIDILLGDTIGEMGLFLRLTEVAFVGRSLFAEGGQNPLEPAMLGCAVLSGGNVQNFRET
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHH
YQMLAKNGSAKIVRDTEMLAKGVNYLLANDDMRRSMIDAGLETVQQMRGALSATMKGLEP
HHHHHCCCCCEEEEHHHHHHHCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YINPLVVKARLEPRPKPEA
HHCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]