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The map label for this gene is yciL [C]

Identifier: 150395641

GI number: 150395641

Start: 448238

End: 449968

Strand: Direct

Name: yciL [C]

Synonym: Smed_0416

Alternate gene names: 150395641

Gene position: 448238-449968 (Clockwise)

Preceding gene: 150395639

Following gene: 150395642

Centisome position: 11.85

GC content: 67.36

Gene sequence:

>1731_bases
ATGACATCCAAAGACAAGCCCACGAGGCCCGGCGGCAAGGCCAAGGGTCGCGACACGAAACCTCATTCCGGCGGCGAGAA
GGCTGCAGTGCGGGCAGCCGGTAGCGCGCCGGCCGCGTCGGCTGCGGGTGCGGAACAGCCACAGCGCATCTCGAAGATAC
TCTCGCGCGCAGGCGTTGCATCGCGCCGCGACGTCGAGCGGATGATCATGGAAGGGCGCGTCAGTCTCAATGGCCTCGTG
CTCGACACACCGGTGGTCAACGCGACGCTCGCCGACAGGATCGAAGTGGACGGCCATCCGATCCGTGGCATCGAACGGAC
GCGGCTTTGGCTCTATCACAAGCCGGGCGGGCTCGTGACGACCAATGCCGATCCGGAAGGCCGGCCGACGGTCTTCGAAA
ACCTGCCCGAGGATCTGCCGCGCGTTCTGTCGATCGGCCGGCTGGACATCAACACCGAAGGCCTGCTGCTCCTGACGAAT
GACGGCGGGCTTGCCCGCGTGCTTGAGCTGCCGTCGACAGGCTGGCTGCGCCGCTACCGCGTGCGCGCACACGGGGAAAT
CGATCAGGCGGCGCTCGACCGCCTGAAAGAGGGTATCGCGGTCGAAGGTGTCCTTTACGGGGCGATCGAGGCTACTCTGG
ATCGGGTACAGGGCTCGAACGTCTGGATCACTATGGGCCTGCGCGAAGGCAAGAATCGTGAGATCAAGAACGTCCTTGGC
GCACTTGGCCTCGACGTAAACCGACTGATCCGCATCTCCTATGGTCCGTTCCAGCTCGGCGAGTTGCCGATTGGTCAGGT
TCAGGAGATTCGCGGCCGAACGCTGCGCGAACAGCTGGGCCCCCGGCTGATCGCCGACGCCAAAGCGAATTTCGACGCGC
CGATCTATAACGACCAGTCGACTGCTGCCGAGCCTGAAGCAGAGCCTGTCGCCGATCACGGCAAGGCGGAATGGGGCAGC
AAGCGCGAGAAGGCCGGAGACAAGCGCGAGCGTGCTCTTGCCCGCCTCGACACCCGCCGCGACGATGGCCGACCGAACGA
CCGAGGCCGCAGTCGTGGAAAGGCGGAGGAACGTCCTGCTCGCCCGCCTCTGAGACGCAACCGCTCTTCCAACGTCTGGA
TGGCGCCGGGTGCGCGTCCGACGGTCGAGAAAAAGCCGAAGGCTGCGGAAGACGAGCTTTCTCCGAAATCCACGCGGCGC
GCGCCGGCGGACGTTAAACGCCGCGACCGTGCTAAGGACCCCGCCACGGCGAAGGCAGCCGGCTTTGATGAGGAGCGTAA
GGGCAAGGCGGGTAAATCCGCCGGCCGCAAGGACGCCGCCGGTGGCTTCGAAAGGCGTCGCCCGCTCGAAGGCGCAGAGC
GGAAATCACGCGACCATGGCGATCGTCCGCCGCGCCGTCCGGAGGGGGAGCGGGCTGCACGCGACCCGGCCGATGACCGC
GCGTCTTCCGGCGGCCGCAAGGAGACCGCTCGGCCACCGCGCGGCGACGGCAAGAAGCGCTTTGCCGACGACCGGCCACG
GGCGAACGCTGGCGGCAAGCCGGCGGCGGCAAACCCCTCCGCCAATCGCTCGTCCGAATCGCGCGCCGGAAAACCCGCAG
GCACCAAACCCTCCGGCGGCAGGCGGCCCGGTGGAGGCAAGCCCGGTAATCGCCCGGGTGGCGGCAAACCCGGTAATCGC
CCTGGTGGCGGCAAATCCTCGGGCAAGGGACCGCAGGGCAGGGGGAAGTAG

Upstream 100 bases:

>100_bases
CTGCATGAAGGGCGCCGTATCCGTCATCTTCGTTCGACAATTTCCTCTTAACCCACGGCCTACGACCTGATAGGACAGCC
GCAAAAGGCAGGCAACACAA

Downstream 100 bases:

>100_bases
CCGGCCGTGCGCATCGTCGGGGGAGAATTCCGCGGCCGCACGCTGGCCACACCGAAGTCGAACGACATCCGCCCGACCAC
GGACCGGACGCGCGAGAGCC

Product: pseudouridine synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MTSKDKPTRPGGKAKGRDTKPHSGGEKAAVRAAGSAPAASAAGAEQPQRISKILSRAGVASRRDVERMIMEGRVSLNGLV
LDTPVVNATLADRIEVDGHPIRGIERTRLWLYHKPGGLVTTNADPEGRPTVFENLPEDLPRVLSIGRLDINTEGLLLLTN
DGGLARVLELPSTGWLRRYRVRAHGEIDQAALDRLKEGIAVEGVLYGAIEATLDRVQGSNVWITMGLREGKNREIKNVLG
ALGLDVNRLIRISYGPFQLGELPIGQVQEIRGRTLREQLGPRLIADAKANFDAPIYNDQSTAAEPEAEPVADHGKAEWGS
KREKAGDKRERALARLDTRRDDGRPNDRGRSRGKAEERPARPPLRRNRSSNVWMAPGARPTVEKKPKAAEDELSPKSTRR
APADVKRRDRAKDPATAKAAGFDEERKGKAGKSAGRKDAAGGFERRRPLEGAERKSRDHGDRPPRRPEGERAARDPADDR
ASSGGRKETARPPRGDGKKRFADDRPRANAGGKPAAANPSANRSSESRAGKPAGTKPSGGRRPGGGKPGNRPGGGKPGNR
PGGGKSSGKGPQGRGK

Sequences:

>Translated_576_residues
MTSKDKPTRPGGKAKGRDTKPHSGGEKAAVRAAGSAPAASAAGAEQPQRISKILSRAGVASRRDVERMIMEGRVSLNGLV
LDTPVVNATLADRIEVDGHPIRGIERTRLWLYHKPGGLVTTNADPEGRPTVFENLPEDLPRVLSIGRLDINTEGLLLLTN
DGGLARVLELPSTGWLRRYRVRAHGEIDQAALDRLKEGIAVEGVLYGAIEATLDRVQGSNVWITMGLREGKNREIKNVLG
ALGLDVNRLIRISYGPFQLGELPIGQVQEIRGRTLREQLGPRLIADAKANFDAPIYNDQSTAAEPEAEPVADHGKAEWGS
KREKAGDKRERALARLDTRRDDGRPNDRGRSRGKAEERPARPPLRRNRSSNVWMAPGARPTVEKKPKAAEDELSPKSTRR
APADVKRRDRAKDPATAKAAGFDEERKGKAGKSAGRKDAAGGFERRRPLEGAERKSRDHGDRPPRRPEGERAARDPADDR
ASSGGRKETARPPRGDGKKRFADDRPRANAGGKPAAANPSANRSSESRAGKPAGTKPSGGRRPGGGKPGNRPGGGKPGNR
PGGGKSSGKGPQGRGK
>Mature_575_residues
TSKDKPTRPGGKAKGRDTKPHSGGEKAAVRAAGSAPAASAAGAEQPQRISKILSRAGVASRRDVERMIMEGRVSLNGLVL
DTPVVNATLADRIEVDGHPIRGIERTRLWLYHKPGGLVTTNADPEGRPTVFENLPEDLPRVLSIGRLDINTEGLLLLTND
GGLARVLELPSTGWLRRYRVRAHGEIDQAALDRLKEGIAVEGVLYGAIEATLDRVQGSNVWITMGLREGKNREIKNVLGA
LGLDVNRLIRISYGPFQLGELPIGQVQEIRGRTLREQLGPRLIADAKANFDAPIYNDQSTAAEPEAEPVADHGKAEWGSK
REKAGDKRERALARLDTRRDDGRPNDRGRSRGKAEERPARPPLRRNRSSNVWMAPGARPTVEKKPKAAEDELSPKSTRRA
PADVKRRDRAKDPATAKAAGFDEERKGKAGKSAGRKDAAGGFERRRPLEGAERKSRDHGDRPPRRPEGERAARDPADDRA
SSGGRKETARPPRGDGKKRFADDRPRANAGGKPAAANPSANRSSESRAGKPAGTKPSGGRRPGGGKPGNRPGGGKPGNRP
GGGKSSGKGPQGRGK

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=225, Percent_Identity=40.8888888888889, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=29.608938547486, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790453, Length=226, Percent_Identity=27.4336283185841, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 61879; Mature: 61748

Theoretical pI: Translated: 11.31; Mature: 11.31

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKDKPTRPGGKAKGRDTKPHSGGEKAAVRAAGSAPAASAAGAEQPQRISKILSRAGVA
CCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
SRRDVERMIMEGRVSLNGLVLDTPVVNATLADRIEVDGHPIRGIERTRLWLYHKPGGLVT
HHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHEECCCCCCCCCCEEEEEEEECCCCEEE
TNADPEGRPTVFENLPEDLPRVLSIGRLDINTEGLLLLTNDGGLARVLELPSTGWLRRYR
CCCCCCCCCHHHHHCHHHHHHHHHCCCEEECCCCEEEEECCCCEEEEEECCCCCHHHHHH
VRAHGEIDQAALDRLKEGIAVEGVLYGAIEATLDRVQGSNVWITMGLREGKNREIKNVLG
HHHCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
ALGLDVNRLIRISYGPFQLGELPIGQVQEIRGRTLREQLGPRLIADAKANFDAPIYNDQS
HHCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCC
TAAEPEAEPVADHGKAEWGSKREKAGDKRERALARLDTRRDDGRPNDRGRSRGKAEERPA
CCCCCCCCCCHHCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHCCC
RPPLRRNRSSNVWMAPGARPTVEKKPKAAEDELSPKSTRRAPADVKRRDRAKDPATAKAA
CCCHHCCCCCCEEECCCCCCCCCCCCCCCHHCCCCHHHCCCCHHHHHHHCCCCCCCHHHC
GFDEERKGKAGKSAGRKDAAGGFERRRPLEGAERKSRDHGDRPPRRPEGERAARDPADDR
CCCHHHCCCCCCCCCCCHHCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCCHHH
ASSGGRKETARPPRGDGKKRFADDRPRANAGGKPAAANPSANRSSESRAGKPAGTKPSGG
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
RRPGGGKPGNRPGGGKPGNRPGGGKSSGKGPQGRGK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSKDKPTRPGGKAKGRDTKPHSGGEKAAVRAAGSAPAASAAGAEQPQRISKILSRAGVA
CCCCCCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
SRRDVERMIMEGRVSLNGLVLDTPVVNATLADRIEVDGHPIRGIERTRLWLYHKPGGLVT
HHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHEECCCCCCCCCCEEEEEEEECCCCEEE
TNADPEGRPTVFENLPEDLPRVLSIGRLDINTEGLLLLTNDGGLARVLELPSTGWLRRYR
CCCCCCCCCHHHHHCHHHHHHHHHCCCEEECCCCEEEEECCCCEEEEEECCCCCHHHHHH
VRAHGEIDQAALDRLKEGIAVEGVLYGAIEATLDRVQGSNVWITMGLREGKNREIKNVLG
HHHCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
ALGLDVNRLIRISYGPFQLGELPIGQVQEIRGRTLREQLGPRLIADAKANFDAPIYNDQS
HHCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCC
TAAEPEAEPVADHGKAEWGSKREKAGDKRERALARLDTRRDDGRPNDRGRSRGKAEERPA
CCCCCCCCCCHHCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHCCC
RPPLRRNRSSNVWMAPGARPTVEKKPKAAEDELSPKSTRRAPADVKRRDRAKDPATAKAA
CCCHHCCCCCCEEECCCCCCCCCCCCCCCHHCCCCHHHCCCCHHHHHHHCCCCCCCHHHC
GFDEERKGKAGKSAGRKDAAGGFERRRPLEGAERKSRDHGDRPPRRPEGERAARDPADDR
CCCHHHCCCCCCCCCCCHHCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCCHHH
ASSGGRKETARPPRGDGKKRFADDRPRANAGGKPAAANPSANRSSESRAGKPAGTKPSGG
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
RRPGGGKPGNRPGGGKPGNRPGGGKSSGKGPQGRGK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]