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The map label for this gene is pbpG [H]

Identifier: 150395430

GI number: 150395430

Start: 229663

End: 231639

Strand: Direct

Name: pbpG [H]

Synonym: Smed_0203

Alternate gene names: 150395430

Gene position: 229663-231639 (Clockwise)

Preceding gene: 150395428

Following gene: 150395431

Centisome position: 6.07

GC content: 64.04

Gene sequence:

>1977_bases
TTGCCAGCTGTTTTCCGTCCGCAATCGCGGCAAAGCGAAGCCCCTGCGCGCGATCGGAGTTCCACGCGAAAGCACCGGCG
CAGCAGGCGGTTGGTGCTGTTGACGAGGGCCGCCGCTGCGGGTGCTGCATGTCTTCTGCTTCTTGCGGCCGTATTTGCCT
GGGCTTTGCGCGACGTGCCGTGGGACGAGATCGCCGAGGGTTCGCTGAAGCCCGTCGTGGTGCTCGAGACGGCCGACGGC
AAGCCGCTGGTGAGCCAGGGGCCGTTTCAGGGCTCCTATGCCGAGCGAAAAGACTTTCCGCCCCATCTTGTCGACGCAGT
TCTCGCTAGGGAGGACCGGCGTTTTTACGAGCATTCCGGGATCGATTTGAGGGGCATCGCCCGAGCCATCTATAGGAATA
TCGGGGCCGGCGAAGTGGTGCAGGGCGGCAGCACGATCACGCAGCAATTGATCAAGATCCTTTATCTCGAGCGCGACCGC
ACCTGGAAACGAAAGATCCAGGAGGCGGTGATCGCCTTCTGGCTGGAAAAGAAGCTCGGCAAGGACGAGATCCTCACCCG
CTATCTGAACAACATCTATCTCGGAGCCGGCGCAACGGGCGTGCCTGCCGCTGCCCGCATCTATTTCGACAAGGACGTGC
AGGAACTCGACGTCGCCGAATCGGCGATGCTTGCGGCGATCATCCGCGCACCCTCGCAGCTGAATCCGCTCACCAATCCC
GAAGGCGCGCGCCAGCAGACGGAACTGGTCCTCGATGCCATGGTGAGGAATGACCTGCTCACCCCGGAACAGGCGAAGAG
CGCGCGCGCCGACTTCGGCAAGTTGCAGCCTTCCAGTCCGGCGGTCCGGTCCGGAAGCTGGTTCGCGGACTGGATCATCC
AGGATGCCCGCGAACTCGCCGGTCCCTATAGAGGCACGATCAAGATCAGGACGACGATGATACCGCGGCTGCAGGTGATC
GCCGAGAAGGTGGTTGCGGAAGCGCTCGGACGCGAAGGCGGCAAGGTGGGCGCGTCGCAGGCTGCCCTCGTGGCGATGAC
GCCGGAGGGCGCCGTGGTCGCCATGGTGGGCGGCCGCGACTATAGCAAGAGCACCTTCAACCGGGCGGTTTCCGCCATGC
GTCAGCCGGGCTCCACCTTCAAGCTGTTCGTCTATTATGCCGCCCTGAAGGCAGGCCTTACGCCTTATGATCCGGTCGAG
GATGCTCCCATCGATATCAACGGCTGGTCTCCGGAAAACTCCGGTGGCGGCTATAGAGGACTCGTCAGCATGGCGGAGGC
CTTTGCGCGTTCGCTCAATGCCGCGACCGTCGCCCTGGCGATGGAGGTCGGCATCGACAAGGTCGCCGCAGCGGCTCGAG
AGCTCGGCATCGACGCCAATCTTTCGGAGACCCCAAGCCTCGCGCTTGGCTCTTCGGAGGTGAACCTGCTCGATCTCACC
GGCGCCTATGCCTCGGTTCGGGCAGGCAAGGCTCCGATCGAGCCCTGGGGCATCGAATCCCTGCACGCTGAGGATCAGCC
GCAAGCCTTCCGGGTTGGACCGTCCACGCAAGCTTCAACGGATGTCAGTCAGTACAGCCCTGATCTGGTCGGAATGCTCA
GGCTCGTCGTCGAACGGGGCACGGGCCGCGAAGCCGACATCGCAGCGCCGGCGGCAGGCAAGACCGGAACCAGCCAGAAC
AACCGTGACGCCTGGTTCGTAGGCTTCACCGAACAATTGGTGGTCGGCGTCTGGGTGGGCAATGACGACGAAACTCCGAT
GAAGGACGTCACCGGCGGCAAACTGCCGGCGCGGATCTGGCGAAACTTCATGACCGCGGCGATGGCGGAAGGCGATCCGC
TGGAGCCGGGCGCCCAGGTGACGGGCCCCGCCCCCGCCTGCAACTTCCGCGCCTGCGCCAGGGCCTATCGTTCGTTCCGG
CCGGACGATTGCACCTTCCAGCCCTATAGCGGGCCGAGGAGGATTTGTGAGAGATAG

Upstream 100 bases:

>100_bases
TGCGCTTTTGAGGCTCGGTCGGGCCATTCGCGAGGAAGTACCTGCCGCCTCGCGCCGAGCGCTGCAGATATTTGGCGGGC
TCCGACGGAATAACACGAGG

Downstream 100 bases:

>100_bases
CGTTTGGGGCGGGGTCTGAAGTGTTGGAAGTGCAGGCCTTCGGCATGCCCCGGCTCGCCTGGTCTCGATTCTTCGAACCG
CCCGTTGCGATCGCATGCCG

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MPAVFRPQSRQSEAPARDRSSTRKHRRSRRLVLLTRAAAAGAACLLLLAAVFAWALRDVPWDEIAEGSLKPVVVLETADG
KPLVSQGPFQGSYAERKDFPPHLVDAVLAREDRRFYEHSGIDLRGIARAIYRNIGAGEVVQGGSTITQQLIKILYLERDR
TWKRKIQEAVIAFWLEKKLGKDEILTRYLNNIYLGAGATGVPAAARIYFDKDVQELDVAESAMLAAIIRAPSQLNPLTNP
EGARQQTELVLDAMVRNDLLTPEQAKSARADFGKLQPSSPAVRSGSWFADWIIQDARELAGPYRGTIKIRTTMIPRLQVI
AEKVVAEALGREGGKVGASQAALVAMTPEGAVVAMVGGRDYSKSTFNRAVSAMRQPGSTFKLFVYYAALKAGLTPYDPVE
DAPIDINGWSPENSGGGYRGLVSMAEAFARSLNAATVALAMEVGIDKVAAAARELGIDANLSETPSLALGSSEVNLLDLT
GAYASVRAGKAPIEPWGIESLHAEDQPQAFRVGPSTQASTDVSQYSPDLVGMLRLVVERGTGREADIAAPAAGKTGTSQN
NRDAWFVGFTEQLVVGVWVGNDDETPMKDVTGGKLPARIWRNFMTAAMAEGDPLEPGAQVTGPAPACNFRACARAYRSFR
PDDCTFQPYSGPRRICER

Sequences:

>Translated_658_residues
MPAVFRPQSRQSEAPARDRSSTRKHRRSRRLVLLTRAAAAGAACLLLLAAVFAWALRDVPWDEIAEGSLKPVVVLETADG
KPLVSQGPFQGSYAERKDFPPHLVDAVLAREDRRFYEHSGIDLRGIARAIYRNIGAGEVVQGGSTITQQLIKILYLERDR
TWKRKIQEAVIAFWLEKKLGKDEILTRYLNNIYLGAGATGVPAAARIYFDKDVQELDVAESAMLAAIIRAPSQLNPLTNP
EGARQQTELVLDAMVRNDLLTPEQAKSARADFGKLQPSSPAVRSGSWFADWIIQDARELAGPYRGTIKIRTTMIPRLQVI
AEKVVAEALGREGGKVGASQAALVAMTPEGAVVAMVGGRDYSKSTFNRAVSAMRQPGSTFKLFVYYAALKAGLTPYDPVE
DAPIDINGWSPENSGGGYRGLVSMAEAFARSLNAATVALAMEVGIDKVAAAARELGIDANLSETPSLALGSSEVNLLDLT
GAYASVRAGKAPIEPWGIESLHAEDQPQAFRVGPSTQASTDVSQYSPDLVGMLRLVVERGTGREADIAAPAAGKTGTSQN
NRDAWFVGFTEQLVVGVWVGNDDETPMKDVTGGKLPARIWRNFMTAAMAEGDPLEPGAQVTGPAPACNFRACARAYRSFR
PDDCTFQPYSGPRRICER
>Mature_657_residues
PAVFRPQSRQSEAPARDRSSTRKHRRSRRLVLLTRAAAAGAACLLLLAAVFAWALRDVPWDEIAEGSLKPVVVLETADGK
PLVSQGPFQGSYAERKDFPPHLVDAVLAREDRRFYEHSGIDLRGIARAIYRNIGAGEVVQGGSTITQQLIKILYLERDRT
WKRKIQEAVIAFWLEKKLGKDEILTRYLNNIYLGAGATGVPAAARIYFDKDVQELDVAESAMLAAIIRAPSQLNPLTNPE
GARQQTELVLDAMVRNDLLTPEQAKSARADFGKLQPSSPAVRSGSWFADWIIQDARELAGPYRGTIKIRTTMIPRLQVIA
EKVVAEALGREGGKVGASQAALVAMTPEGAVVAMVGGRDYSKSTFNRAVSAMRQPGSTFKLFVYYAALKAGLTPYDPVED
APIDINGWSPENSGGGYRGLVSMAEAFARSLNAATVALAMEVGIDKVAAAARELGIDANLSETPSLALGSSEVNLLDLTG
AYASVRAGKAPIEPWGIESLHAEDQPQAFRVGPSTQASTDVSQYSPDLVGMLRLVVERGTGREADIAAPAAGKTGTSQNN
RDAWFVGFTEQLVVGVWVGNDDETPMKDVTGGKLPARIWRNFMTAAMAEGDPLEPGAQVTGPAPACNFRACARAYRSFRP
DDCTFQPYSGPRRICER

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=531, Percent_Identity=33.3333333333333, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1788867, Length=563, Percent_Identity=31.2611012433393, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87082258, Length=293, Percent_Identity=34.4709897610922, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789601, Length=155, Percent_Identity=38.0645161290323, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 71156; Mature: 71024

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVFRPQSRQSEAPARDRSSTRKHRRSRRLVLLTRAAAAGAACLLLLAAVFAWALRDVP
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
WDEIAEGSLKPVVVLETADGKPLVSQGPFQGSYAERKDFPPHLVDAVLAREDRRFYEHSG
HHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
IDLRGIARAIYRNIGAGEVVQGGSTITQQLIKILYLERDRTWKRKIQEAVIAFWLEKKLG
CCHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
KDEILTRYLNNIYLGAGATGVPAAARIYFDKDVQELDVAESAMLAAIIRAPSQLNPLTNP
HHHHHHHHHHHEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHCCCCCCC
EGARQQTELVLDAMVRNDLLTPEQAKSARADFGKLQPSSPAVRSGSWFADWIIQDARELA
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GPYRGTIKIRTTMIPRLQVIAEKVVAEALGREGGKVGASQAALVAMTPEGAVVAMVGGRD
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCC
YSKSTFNRAVSAMRQPGSTFKLFVYYAALKAGLTPYDPVEDAPIDINGWSPENSGGGYRG
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCHHH
LVSMAEAFARSLNAATVALAMEVGIDKVAAAARELGIDANLSETPSLALGSSEVNLLDLT
HHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEC
GAYASVRAGKAPIEPWGIESLHAEDQPQAFRVGPSTQASTDVSQYSPDLVGMLRLVVERG
CCHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCHHHHHHHHHHHHCC
TGREADIAAPAAGKTGTSQNNRDAWFVGFTEQLVVGVWVGNDDETPMKDVTGGKLPARIW
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCHHHHH
RNFMTAAMAEGDPLEPGAQVTGPAPACNFRACARAYRSFRPDDCTFQPYSGPRRICER
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
PAVFRPQSRQSEAPARDRSSTRKHRRSRRLVLLTRAAAAGAACLLLLAAVFAWALRDVP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
WDEIAEGSLKPVVVLETADGKPLVSQGPFQGSYAERKDFPPHLVDAVLAREDRRFYEHSG
HHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
IDLRGIARAIYRNIGAGEVVQGGSTITQQLIKILYLERDRTWKRKIQEAVIAFWLEKKLG
CCHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
KDEILTRYLNNIYLGAGATGVPAAARIYFDKDVQELDVAESAMLAAIIRAPSQLNPLTNP
HHHHHHHHHHHEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCHHCCCCCCC
EGARQQTELVLDAMVRNDLLTPEQAKSARADFGKLQPSSPAVRSGSWFADWIIQDARELA
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GPYRGTIKIRTTMIPRLQVIAEKVVAEALGREGGKVGASQAALVAMTPEGAVVAMVGGRD
CCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCC
YSKSTFNRAVSAMRQPGSTFKLFVYYAALKAGLTPYDPVEDAPIDINGWSPENSGGGYRG
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCHHH
LVSMAEAFARSLNAATVALAMEVGIDKVAAAARELGIDANLSETPSLALGSSEVNLLDLT
HHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEC
GAYASVRAGKAPIEPWGIESLHAEDQPQAFRVGPSTQASTDVSQYSPDLVGMLRLVVERG
CCHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCHHHHHHHHHHHHCC
TGREADIAAPAAGKTGTSQNNRDAWFVGFTEQLVVGVWVGNDDETPMKDVTGGKLPARIW
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCHHHHH
RNFMTAAMAEGDPLEPGAQVTGPAPACNFRACARAYRSFRPDDCTFQPYSGPRRICER
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]