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The map label for this gene is aes [H]

Identifier: 150395412

GI number: 150395412

Start: 204218

End: 205198

Strand: Direct

Name: aes [H]

Synonym: Smed_0185

Alternate gene names: 150395412

Gene position: 204218-205198 (Clockwise)

Preceding gene: 150395411

Following gene: 150395413

Centisome position: 5.4

GC content: 68.5

Gene sequence:

>981_bases
ATGAGCGCGGCGCCGGACCTGTCGCGGCTGTCGCCGGAAATGGCGGCGCTGCTCCTGCGCGTGGCTGCCGAAACGGGGCC
GCAGGTCGATCCCACGACCTTGCCGCCGGCCGAGGGCCGCTCGCTTTCGGAAGAGGGCAACCGGCGGTGGAACGTCGGGC
TGCCGGAAATGGCGGCGGTCGGAGAGGCTTTTGTCGAGGCGGACGCGGCTATCGGCTCGGCGCGCCTGCGCCTCAAGATA
CTGGTGCCGCCGGGCGCCGGCTCCGGTGCGGTGGTCTTTGTGCATGGCGGCGGCTTTGCCTTCTGCAGCCCGGAATCGCA
TGAGCGCTGTGCGCGCGTTCTCGCGCTGGAATGCGGCCTGCCGGTGCTCCTGCCGGATTATCGCCTGGCGCCGGAAAATC
CCTATCCCGCCGGCCTGATGGATGTTGTCGCGACGATCCGCAACGCATTTGCAGTCTCGGCGCCCTTCGGCGTGGCGGCG
GGCCCGCTACTCCTTTCCGGCGATTCGGCGGGCGCCAATCTTGCACTCGCCGCACTGTTGCACGAAGCGGCGGCGGGGCG
CCCGCGAATGGCTGGTGCACTCCTTTTCTACGGAACCTATGCCGGCGATTTCCGCACGGAGTCCTATCGAAATTTCGAGA
ATGGGCCGGGTTTGACGACGGCGAAGATGCAGCGCTACTGGCGCTGGTATGTCGGCGACCGCGACGTTTCGGCTGATCCG
CTCGCATGTCCGCTTCTCGCCTCCGATGAGGCCTTGGCGGCTCTGCCGCCGCTTCATCTGATGGCGGCGGGCGTCGACCC
GCTGCTGTCGGACAGCATCGCGCTGCACCAACGCCTGAAGCAACTCGGGCGGGGCGAGGCGCTGATGGTGGTGCCCGGCG
TCACGCACGGCTTCTTGCAGAACACCCTCGATCTCGCCGCCGCGCGGGAGGCGCTTGCGAAGGCAGGTGCCGAAGCCCGG
CGAATGGCAGGCGCGAACTGA

Upstream 100 bases:

>100_bases
CGATCGAAAGCGGCGATGTCGCGAAGGCCGGCGCAGCCCTGCGCCATCACATCGCGACGATCGTTGATCTGGGCCCCGAC
GTCTTCCCGGATGCGACCCA

Downstream 100 bases:

>100_bases
AGCGATTTTCAAAGGAGGAGACAATGAAAATCTGGAAAATAGGCACCGCTCTCGCGGCATCGCTTCTGGCAAGCACCGCA
TCGGCGGAAACCGTTCGTTT

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSAAPDLSRLSPEMAALLLRVAAETGPQVDPTTLPPAEGRSLSEEGNRRWNVGLPEMAAVGEAFVEADAAIGSARLRLKI
LVPPGAGSGAVVFVHGGGFAFCSPESHERCARVLALECGLPVLLPDYRLAPENPYPAGLMDVVATIRNAFAVSAPFGVAA
GPLLLSGDSAGANLALAALLHEAAAGRPRMAGALLFYGTYAGDFRTESYRNFENGPGLTTAKMQRYWRWYVGDRDVSADP
LACPLLASDEALAALPPLHLMAAGVDPLLSDSIALHQRLKQLGRGEALMVVPGVTHGFLQNTLDLAAAREALAKAGAEAR
RMAGAN

Sequences:

>Translated_326_residues
MSAAPDLSRLSPEMAALLLRVAAETGPQVDPTTLPPAEGRSLSEEGNRRWNVGLPEMAAVGEAFVEADAAIGSARLRLKI
LVPPGAGSGAVVFVHGGGFAFCSPESHERCARVLALECGLPVLLPDYRLAPENPYPAGLMDVVATIRNAFAVSAPFGVAA
GPLLLSGDSAGANLALAALLHEAAAGRPRMAGALLFYGTYAGDFRTESYRNFENGPGLTTAKMQRYWRWYVGDRDVSADP
LACPLLASDEALAALPPLHLMAAGVDPLLSDSIALHQRLKQLGRGEALMVVPGVTHGFLQNTLDLAAAREALAKAGAEAR
RMAGAN
>Mature_325_residues
SAAPDLSRLSPEMAALLLRVAAETGPQVDPTTLPPAEGRSLSEEGNRRWNVGLPEMAAVGEAFVEADAAIGSARLRLKIL
VPPGAGSGAVVFVHGGGFAFCSPESHERCARVLALECGLPVLLPDYRLAPENPYPAGLMDVVATIRNAFAVSAPFGVAAG
PLLLSGDSAGANLALAALLHEAAAGRPRMAGALLFYGTYAGDFRTESYRNFENGPGLTTAKMQRYWRWYVGDRDVSADPL
ACPLLASDEALAALPPLHLMAAGVDPLLSDSIALHQRLKQLGRGEALMVVPGVTHGFLQNTLDLAAAREALAKAGAEARR
MAGAN

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=318, Percent_Identity=29.2452830188679, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71996133, Length=154, Percent_Identity=35.7142857142857, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=174, Percent_Identity=31.6091954022989, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI72001146, Length=133, Percent_Identity=34.5864661654135, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17567059, Length=292, Percent_Identity=23.6301369863014, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34009; Mature: 33878

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAPDLSRLSPEMAALLLRVAAETGPQVDPTTLPPAEGRSLSEEGNRRWNVGLPEMAAV
CCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCEECCCCCHHHHH
GEAFVEADAAIGSARLRLKILVPPGAGSGAVVFVHGGGFAFCSPESHERCARVLALECGL
HHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHCCC
PVLLPDYRLAPENPYPAGLMDVVATIRNAFAVSAPFGVAAGPLLLSGDSAGANLALAALL
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCHHHHHHHH
HEAAAGRPRMAGALLFYGTYAGDFRTESYRNFENGPGLTTAKMQRYWRWYVGDRDVSADP
HHHHCCCCCHHCEEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
LACPLLASDEALAALPPLHLMAAGVDPLLSDSIALHQRLKQLGRGEALMVVPGVTHGFLQ
CCCCEECCCCHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
NTLDLAAAREALAKAGAEARRMAGAN
HHHHHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SAAPDLSRLSPEMAALLLRVAAETGPQVDPTTLPPAEGRSLSEEGNRRWNVGLPEMAAV
CCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCEECCCCCHHHHH
GEAFVEADAAIGSARLRLKILVPPGAGSGAVVFVHGGGFAFCSPESHERCARVLALECGL
HHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHCCC
PVLLPDYRLAPENPYPAGLMDVVATIRNAFAVSAPFGVAAGPLLLSGDSAGANLALAALL
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCHHHHHHHH
HEAAAGRPRMAGALLFYGTYAGDFRTESYRNFENGPGLTTAKMQRYWRWYVGDRDVSADP
HHHHCCCCCHHCEEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
LACPLLASDEALAALPPLHLMAAGVDPLLSDSIALHQRLKQLGRGEALMVVPGVTHGFLQ
CCCCEECCCCHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
NTLDLAAAREALAKAGAEARRMAGAN
HHHHHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA