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The map label for this gene is pyrC [H]

Identifier: 150395334

GI number: 150395334

Start: 117052

End: 118095

Strand: Direct

Name: pyrC [H]

Synonym: Smed_0106

Alternate gene names: 150395334

Gene position: 117052-118095 (Clockwise)

Preceding gene: 150395333

Following gene: 150395335

Centisome position: 3.1

GC content: 62.07

Gene sequence:

>1044_bases
ATGCAACACCTCGTCATCCGCCGCCCGGACGATTGGCACCTTCACCTGCGCGACGGCGGCATGCTGCGCGGTGTCATCGC
CGATACGAGCCGCCATTTCGCGCGCGCCATAATCATGCCCAATCTGGTGCCACCGGTGGTCACCTCCGCCGACGCGGCCA
CCTATCGCGAGCGTATCCTTGCCGCCATTCCCGCAGGGCACCGGTTCGAGCCCCTGATGACGCTTTATCTCACCGAGGGC
ACCGATCCCGATGATGTGGAAGAGGGCTTCAGGAGCGGCCTCGTCAAGGCCGTCAAGCTCTATCCGGCGGGCGCCACCAC
CAACTCGTCGAGCGGCGTGCGCGATATCGACAAGGCGATGCCAGTGCTCGAGCGCATGGCCGAGGTCGGCGTGCCGCTCT
GCGTCCATGGTGAGGTGACGACGGCGGACGTAGACATTTTCGACCGCGAAGCTGTTTTCATCGAAACCGTTCTCGACCCG
CTCCGCCGGCGATTGCCCGATCTCAGGATCACCATGGAGCATGTGACGACGAAGGACGGCGTCGACTACATCCGGGAACA
TGCGGTCAATCTTGCCGGGTCGATCACCACTCATCACCTGATCATCAATCGCAACGCCATCCTCGTCGGCGGTATCAAGC
CGCATTACTACTGCCTGCCGGTGGCAAAACGCGAGATGCACCGGTTGGCGCTGCGCGAAGCGGCGATCTCTGGCGACGTA
CGCTTTTTCCTGGGAACGGACTCGGCTCCGCATGTCGATCCGCTCAAGGAATGCGCCTGCGGTTGTGCCGGGATCTACAC
ATCCATCAATTCATTGAGCTGCCTTGCCCATGTCTTCGAGGAGGAGGGCGCGCTCGACCGGCTGGAAGCCTTCACCTCGC
TGAACGGTCCCGCCTGGTACGGATTGCCGCCAAACGAAGAGACGATCACGCTACGCAAACAGGAAGAGCCGGTCGGCTAT
CCCGCCAAAATCGAGACTGAAGCCGGTCCGGTGACGGTTTTCGACCCGATGTTCCCGCTCTACTGGACGGTGATCGAAGC
CTAA

Upstream 100 bases:

>100_bases
GAGGCTCGGTTTCACCACATGAGTTGCCGCCCATCGAGCGCACCGGTACATCCTGACACCGGCCGCGCGGAACGGGCGCG
AGCATCGGGAGAATGCTGAT

Downstream 100 bases:

>100_bases
GGGTTTGTGTTGTCGCGTGACTCTCCCGAAAAGCGGTTGGCATATTTCGGGGTCACGCCAGGAAGGAGAATTCATGTTTT
CGAACGCGTTCACGGAGAAG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MQHLVIRRPDDWHLHLRDGGMLRGVIADTSRHFARAIIMPNLVPPVVTSADAATYRERILAAIPAGHRFEPLMTLYLTEG
TDPDDVEEGFRSGLVKAVKLYPAGATTNSSSGVRDIDKAMPVLERMAEVGVPLCVHGEVTTADVDIFDREAVFIETVLDP
LRRRLPDLRITMEHVTTKDGVDYIREHAVNLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREMHRLALREAAISGDV
RFFLGTDSAPHVDPLKECACGCAGIYTSINSLSCLAHVFEEEGALDRLEAFTSLNGPAWYGLPPNEETITLRKQEEPVGY
PAKIETEAGPVTVFDPMFPLYWTVIEA

Sequences:

>Translated_347_residues
MQHLVIRRPDDWHLHLRDGGMLRGVIADTSRHFARAIIMPNLVPPVVTSADAATYRERILAAIPAGHRFEPLMTLYLTEG
TDPDDVEEGFRSGLVKAVKLYPAGATTNSSSGVRDIDKAMPVLERMAEVGVPLCVHGEVTTADVDIFDREAVFIETVLDP
LRRRLPDLRITMEHVTTKDGVDYIREHAVNLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREMHRLALREAAISGDV
RFFLGTDSAPHVDPLKECACGCAGIYTSINSLSCLAHVFEEEGALDRLEAFTSLNGPAWYGLPPNEETITLRKQEEPVGY
PAKIETEAGPVTVFDPMFPLYWTVIEA
>Mature_347_residues
MQHLVIRRPDDWHLHLRDGGMLRGVIADTSRHFARAIIMPNLVPPVVTSADAATYRERILAAIPAGHRFEPLMTLYLTEG
TDPDDVEEGFRSGLVKAVKLYPAGATTNSSSGVRDIDKAMPVLERMAEVGVPLCVHGEVTTADVDIFDREAVFIETVLDP
LRRRLPDLRITMEHVTTKDGVDYIREHAVNLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREMHRLALREAAISGDV
RFFLGTDSAPHVDPLKECACGCAGIYTSINSLSCLAHVFEEEGALDRLEAFTSLNGPAWYGLPPNEETITLRKQEEPVGY
PAKIETEAGPVTVFDPMFPLYWTVIEA

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=318, Percent_Identity=58.4905660377358, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323452, Length=328, Percent_Identity=31.7073170731707, Blast_Score=152, Evalue=7e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 38366; Mature: 38366

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLVIRRPDDWHLHLRDGGMLRGVIADTSRHFARAIIMPNLVPPVVTSADAATYRERIL
CCCEEEECCCCCEEEEECCCEEEEHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHH
AAIPAGHRFEPLMTLYLTEGTDPDDVEEGFRSGLVKAVKLYPAGATTNSSSGVRDIDKAM
HHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHH
PVLERMAEVGVPLCVHGEVTTADVDIFDREAVFIETVLDPLRRRLPDLRITMEHVTTKDG
HHHHHHHHCCCCEEECCCEEEEECEEECCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCCH
VDYIREHAVNLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREMHRLALREAAISGDV
HHHHHHHHHHHHCCCEEEEEEEECCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHCCCE
RFFLGTDSAPHVDPLKECACGCAGIYTSINSLSCLAHVFEEEGALDRLEAFTSLNGPAWY
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEE
GLPPNEETITLRKQEEPVGYPAKIETEAGPVTVFDPMFPLYWTVIEA
CCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCHHHHHEEECC
>Mature Secondary Structure
MQHLVIRRPDDWHLHLRDGGMLRGVIADTSRHFARAIIMPNLVPPVVTSADAATYRERIL
CCCEEEECCCCCEEEEECCCEEEEHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHH
AAIPAGHRFEPLMTLYLTEGTDPDDVEEGFRSGLVKAVKLYPAGATTNSSSGVRDIDKAM
HHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHH
PVLERMAEVGVPLCVHGEVTTADVDIFDREAVFIETVLDPLRRRLPDLRITMEHVTTKDG
HHHHHHHHCCCCEEECCCEEEEECEEECCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCCH
VDYIREHAVNLAGSITTHHLIINRNAILVGGIKPHYYCLPVAKREMHRLALREAAISGDV
HHHHHHHHHHHHCCCEEEEEEEECCEEEECCCCCCEEEECHHHHHHHHHHHHHHHHCCCE
RFFLGTDSAPHVDPLKECACGCAGIYTSINSLSCLAHVFEEEGALDRLEAFTSLNGPAWY
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEE
GLPPNEETITLRKQEEPVGYPAKIETEAGPVTVFDPMFPLYWTVIEA
CCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]